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Issue: Vol. 477 No. 1: 22 December 2020
Type: Article
Published: 2020-12-22
DOI: 10.11646/phytotaxa.477.1.4
Page range: 60–70
Abstract views: 52
PDF downloaded: 1

The taxonomic revision of Pleione formosana (Arethuseae, Epidendroideae, Orchidaceae) based on molecular, morphological and cytological analyses

Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou 350002, China Fujian Ornamental Plant Germplasm Resources Innovation & Engineering Application Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou 350002, China Fujian Ornamental Plant Germplasm Resources Innovation & Engineering Application Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou 350002, China Fujian Ornamental Plant Germplasm Resources Innovation & Engineering Application Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou 350002, China Fujian Ornamental Plant Germplasm Resources Innovation & Engineering Application Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou 350002, China Fujian Ornamental Plant Germplasm Resources Innovation & Engineering Application Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou 350002, China Fujian Ornamental Plant Germplasm Resources Innovation & Engineering Application Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou 350002, China Fujian Ornamental Plant Germplasm Resources Innovation & Engineering Application Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou 350002, China Fujian Ornamental Plant Germplasm Resources Innovation & Engineering Application Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Monocots cpDNA network nrITS ornamental phylogenetic

Abstract

Due to the small degree of morphological and karyotype variations, the Pleione formosana, a well-known and valuable ornamental orchid, was treated as one member of the P. bulbocodioides complex including P. bulbocodioides, P. pleionoides and P. limprichtii, as well. The taxonomy of this complex remains problematic. P. hui was described in Anfu, Jiangxi, China, but was treated as synonym of P. formosana latter, making its taxonomic status remains controversy. In this study, we used molecular, morphological and cytological analyses to identify the relationship of P. formosana and other related species. Results base on phylogenetic analyses of chloroplast DNA and nuclear ribosomal Internal Transcribed Spacer sequence datasets indicated that Anfu samples were obviously separated from the P. formosana accessions. Network analysis showed three different groups: the P. formosana group, the P. bulbocodioides complex and the hybrid group. Morphological analysis also showed that Anfu population was significantly different from other P. formosana populations. Results of chromosome ploidy analyses showed that P. formosana was diploid and Anfu population was tetraploid, indicating that they are not the same species. In conclusion, we suggest to exclude P. formosana from P. bulbocodiodes complex, and restore the speceies rank of P. hui.

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