Tylopilus hayatae Rodríguez-Ramírez & Luna-Vega, sp. nov., is an ectomycorrhizal fungus found in relict Mexican beech forest (Fagus grandifolia subsp. mexicana) in the mountains of Hidalgo State, Sierra Madre Oriental, in eastern Mexico. We propose this new species based on morphological and molecular data evidence. Macro- and microscopic descriptions including illustrations, photographs and scanning electron micrographs of the basidiospores are presented.