Revision of the Synechococcales (Cyanobacteria) through recognition of four families including Oculatellaceae fam. nov. and Trichocoleaceae fam. nov. and six new genera containing 14 species

TRUC MAI; JEFFREY R. JOHANSEN; NICOLE PIETRASIAK; MARKÉTA BOHUNICKÁ; MICHAEL P. MARTIN

doi:10.11646/phytotaxa.365.1.1

Issue: Vol. 365 No. 1: 16 August 2018

Type: Article

Published: 2018-08-16

DOI: 10.11646/phytotaxa.365.1.1

Page range: 1–59

Abstract views: 35

PDF downloaded: 9

**Revision of the Synechococcales (Cyanobacteria) through recognition of four families including Oculatellaceae fam. nov. and Trichocoleaceae fam. nov. and six new genera containing 14 species**

TRUC MAI⁺⁻
JEFFREY R. JOHANSEN⁺⁻
NICOLE PIETRASIAK⁺⁻
MARKÉTA BOHUNICKÁ⁺⁻
MICHAEL P. MARTIN⁺⁻

Department of Biology, John Carroll University, 1 John Carroll Blvd., University Heights, Ohio 44118, USA Department of Plant and Environmental Sciences, New Mexico State University, Skeen Hall Room N127, P.O Box 30003 MSC 3Q, Las Cruces, New Mexico 88003, USA

Department of Biology, John Carroll University, 1 John Carroll Blvd., University Heights, Ohio 44118, USA Department of Botany, Faculty of Science, University of South Bohemia, 31 Branišovská, 37005 České Budějovice, Czech Republic

Department of Plant and Environmental Sciences, New Mexico State University, Skeen Hall Room N127, P.O Box 30003 MSC 3Q, Las Cruces, New Mexico 88003, USA

Department of Biology, Faculty of Science, University of Hradec Králové, Rokitanského 62, 500 03 Hradec Králové, Czech Republic

Department of Biology, John Carroll University, 1 John Carroll Blvd., University Heights, Ohio 44118, USA

Algae Pegethrix Drouetiella Cartusia Tildeniella Komarkovaea Kaiparowitsia Timaviella Oculatellaceae Leptolyngbyaceae Prochlorotrichaceae Trichocoleaceae 16S rRNA phylogeny rpoC1 phylogeny 16S rRNA synapomorphy cyanobacteria taxonomy

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Abstract

A total of 48 strains of thin, filamentous cyanobacteria in Synechococcales were studied by sequencing 16S rRNA and rpoC1 sequence fragments. We also carefully characterized a subset of these by morphology. Phylogenetic analysis of the 16S rRNA gene data using Bayesian inference of a large Synechococcales alignment (345 OTU’s) was in agreement with the phylogeny based on the rpoC1 gene for 59 OTU’s. Both indicated that the large family-level grouping formerly classified as the Leptolyngbyaceae could be further divided into four family-level clades. Two of these family-level clades have been recognized previously as Leptolyngbyaceae and Prochlorotrichaceae. Oculatellaceae fam. nov. and Trichocoleaceae fam. nov. are proposed for the other two families. The Oculatellaceae was studied in greater detail, and six new genera containing 14 species were characterized and named. These new taxa are: Pegethrix botrychoides, P. olivacea, P. convoluta, P. indistincta, Drouetiella lurida, D. hepatica, D. fasciculata, Cartusia fontana, Tildeniella torsiva, T. nuda, Komarkovaea angustata, Kaiparowitsia implicata, Timaviella obliquedivisa, and T. radians.

References

Abed R.M.M., Garcia-Pichel, F. & Hernández-Mariné, M. (2002) Polyphasic characterisation of benthic, moderately halophilic, moderately thermophilic cyanobacteria with very thin trichomes and the proposal of Halomicronema excentricum gen.nov., sp. nov. Archives of Microbiology 177 (5): 361–370. https://doi.org/10.1007/s00203-001-0390-2Adeolu, M, & Gupta, R.S. (2013) Phylogenomics and molecular signatures for the order Neisseriales: proposal for division of the order Neisseriales into the emended family Neisseriaceae and Chromobacteriaceae fam. nov. Antonie van Leeuwenhoek 104: 1–24. https://doi.org/10.1007/s10482-013-9920-6Albertano, P. & Grilli-Caiola, M. (1988) Structural and ultrastructural characters of a red biodeteriorating Lyngbya sp. in culture. Archiv für Hydrobiologie, Supplement 80: 55–57.An, G.S. (1992) Saline algae at the West Sea coast in North Korea. Fragmenta Floristica et Geobotanica 37: 417–424.Anagnostidis, K. & Komárek, J. (1985) Modern approach to the classification system of cyanophytes. 3. Oscillatoriales. Archiv für Hydrobiologie, Supplement 80: 327–472.Anagnostidis, K. (2001) Nomenclatural changes in cyanoprokaryotic order Oscillatoriales. Preslia 73: 359–375.Andersen, R. A. (2013) Advances in algal cell biology. Phycologia 52: 383–384. https://doi.org/10.2216/13-BR4.1Baran, R., Ivanova, N.N., Jose, N., Garcia-Pichel, F., Kyrpides, N.C., Gugger, M. & Northen T.R. (2013) Functional genomics of novel secondary metabolites from diverse cyanobacteria using untargeted metabolomics. Marine Drugs 11 (10): 3617–3631. https://doi.org/10.3390/md11103617Berrendero-Gómez, E., Johansen, J.R., Kaštovský, J., Bohunická, M. & Čapková (2016) Macrochaete gen. nov. (Nostocales, Cyanobacteria), a taxon morphologically and molecularly distinct from Calothrix. Journal of Phycology 52: 638–655. https://doi.org/10.1111/jpy.12425Bevilacqua, P.C. & Blose, J.M. (2008) Structures, kinetics, thermodynamics and biological functions of RNA hairpins. Annual Review of Physical Chemistry 59: 79–103. https://doi.org/10.1146/annurev.physchem.59.032607.093743Bittencourt-Oliveira, M.C., Moura, A.N., Oliveira, M.C. & Massola Jr., N.S. (2009) Geitlerinema species (Oscillatoriales, Cyanobacteria) revealed by cellular morphology, ulstrastructure and DNA sequencing. Journal of Phycology 45: 716–725. https://doi.org/10.1111/j.1529-8817.2009.00682.xBohunická, M., Johansen, J.R. & Fucíková, K. (2011) Tapinothrix clintonii sp. nov. (Pseudanabaenaceae, Cyanobacteria), a new species at the nexus of five genera. Fottea 11: 127–140. https://doi.org/10.5507/fot.2011.013Bohunická, M., Pietrasiak, N., Johansen, J.R., Gómez, E.B., Hauer, T., Gaysina, L.A. & Lukešová, A. (2015) Rohotiella, gen. nov. (Nostocales, Cyanobacteria)-a tapering and branching cyanobacteria of the family Nostocaceae. Phytotaxa 197 (2): 84–103. https://doi.org/10.11646/phytotaxa.197.2.2Borge, O. (1923) Beiträge zur Algenflora von Schweden III. Arkiv för Botanik 18: 1–34.Boyer, S.L., Flechtner, V.R. & Johansen, J.R. (2001) Is the 16S-23S rRNA internal transcribed spacer region a good tool for use in molecular systematics and population genetics? A case study in cyanobacteria. Molecular Biology and Evolution 18: 1057–1069. https://doi.org/10.1093/oxfordjournals.molbev.a003877Brown, I.I., Bryant, Casamatta, D.A., Thomas-Keprta, K.L., Sarkisova, S.A., Shen, G., Graham, J.E., Boyd, E.S., Peters, J.W., Garrison, D.H. & McKay, D.S. (2010) Polyphasic characterization of a thermotolerant siderophillic filamentous cyanobacterium that produces intracellular iron deposits. Applied and Environmental Microbiology 76 (19): 6664–6672. https://doi.org/10.1128/AEM.00662-10Bruno, L., Bili, D., Bellezza, S. & Albertano, P. (2009) Cytomorphological and genetic characterization of troglobitic Leptolyngbya strains isolated from Roman hypogea. Applied and Environmental Microbiology 75: 608–617. https://doi.org/10.1128/AEM.01183-08Burke, D.J., Kretzer, A.M., Rygiewicz, P.T. & Topa, A.M. (2006) Soil bacterial diversity in a loblolly pine plantation: influence of ecotomycorrhizas and fertilization. FEMS Microbial Ecology 57: 409–419. https://doi.org/10.1111/j.1574-6941.2006.00125.xBurger-Wiersma, T., Stal, L.J. & Mur, L. (1989) Prochlorothrix hollandica gen. nov., sp. nov., a filamentous oxygenic photoautotroph procaryote containing chlorophylls a and b: assignment to Prochlorotrichaceae fam.nov. and order Prochlorales. Florenzano, Balloni and Materassi 1986, with Emendation of the Ordinal Description. International Journal of Systematic Bacteriology 39: 250–257. https://doi.org/10.1099/00207713-39-3-250Carmichael, W.W. (1986) Isolation, culture and toxicity testing of toxic freshwater cyanobacteria (blue-green algae). Fundamental Research in Homogeneous Catalysis 3: 1249–1262.Case, R.J., Boucher, Y., Dahllöt, I., Holmström, C., Doolittle, W.F. & Kjelleberg, S. (2007) Use of 16S rRNA and rpoB genes as molecular markers for microbial ecology studies. Applied and Environmental Microbiology 73: 278–288. https://doi.org/10.1128/AEM.01177-06Casamatta, D.A., Johansen J.R., Vis, M.L. & Broadwater S.T. (2005) Molecular and morphological characterization of ten polar and near-polar strains within the Oscillatoriales (Cyanobacteria). Journal of Phycology 41: 421–438. https://doi.org/10.1111/j.1529-8817.2005.04062.xCedegren, R., Gray, M.W., Abel, Y. & Sankoff, D. (1988) The evolutionary relationships among known life forms. Journal of Molecular Evolution 28: 98–112. https://doi.org/10.1007/BF02143501Coates, R.C., Podell, S., Korobeynikov, A., Lapidus, A., Pevzner, P., Sherman, D.H., Gerwick, L. & Gerwick, W.H. (2014) Characterization of cyanobacterial hydrocarbon composition and distribution of biosynthetic pathways. PLOS one. https://doi.org/10.1371/journal.pone.0085140Crouan, P.L. & Crouan, H.M. (1867) Contenant les descriptions de 360 espèces nouvelles de sporogames, de nombreuses observations et une synonymic des plantes cellulaires et vasculaires qui croissent spontanément dans ce département, accompagnées de trente-deux planches où est représentée l’organographie, faite sur l’état vif, des fruits et des tissus de 198 genres d’algues avecla plante grandeur naturelle ou réduite plus une planche supplémentaire où sont figures 24 champignons nouveaux. Florule du Finistère : 111, Plate 2:15.Crow, W.B. (1927) The generic characters of Arthrospira and Spirulina. Transactions of the American Microscopial Society 46: 139–148. https://doi.org/10.2307/3221656Dadheech, P.W., Mahmoud, H., Kotut, K. & Krienitz, L. (2012) Haloleptolyngbya alcalis gen.et sp. nov., a new filamentous cyanobacterium from the soda lake Nakuru, Kenya. Hydrobiologia 637: 269–283. https://doi.org/10.1007/s10750-012-1080-6Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest2: more models, new heuristics and parallel computing. Nature Methods 9: 772. https://doi.org/10.1038/nmeth.2109Drouet, F. (1942) Studies in Myxophyceae. I. Botanical Series of the Field Museum of Natural History 20: 136–137. https://doi.org/10.5962/bhl.title.2283Dvořák, P., Hindák, F., Hašler, Hindáková, A. & Poulíčková, A. (2014) Morphological and molecular studies of Neosynechococcus sphagnicola, gen. et sp. nov. (Cyanobacteria, Synechococcales). Phytotaxa 170 (1): 024–034. https://doi.org/10.11646/phytotaxa.170.1.3Dvořák, P., Jahodářová, E., Hašler, P., Gusev, E. & Pouličková, A. (2015) A new tropical cyanobacterium Pinocchia polymorpha gen. et sp. nov. derived from the genus Pseudanabaena. Fottea 15: 113–120. https://doi.org/10.5507/fot.2015.010Erwin, P.M. & Thacker, R.W. (2008) Cryptic diversity of the symbiotic cyanobacterium Synechococcus spongiarum among sponge host. Molecular Ecology 17: 2937–2947. https://doi.org/10.1111/j.1365-294X.2008.03808.xFergusson, K.M. & Saint, C.P. (2000) Molecular phylogeny of Anabaena circinalis and its identification in environmental samples by PCR. Applied and Environmental Microbiology 66: 4145–4148. https://doi.org/10.1128/AEM.66.9.4145-4148.2000Forti, A. (1907) Vol. V. Sylloge Myxophycearum. In: De Toni (Ed.) Sylloge algarum omnium hucusque cognitarum. Sumptibus auctoris, Patavii, pp. 1–761.Fučiková, K., Lewis, P.O & Lewis, L.A. (2014) Putting incertae sedis taxa in their place: a proposal for ten new and three new genera in Sphaeropleales (Chlorophyceae, Chlorophyta). Journal of Phycology 50: 14–25. https://doi.org/10.1111/jpy.12118Furtado, A.L.F.F., Calijuri, M.D.C., Lorenzi, A.S., Honda, R.Y., Genuário, D.B. & Fiore, M.F. (2009) Morphological and molecular characterization of cyanobacteria from a Brazilian facultative wastewater stabilization pond and evaluation of microcystin production. Hydrobiologia 627: 195–209. https://doi.org/10.1007/s10750-009-9728-6Gaget, V., Gribaldo, S. & Tandeau de Marsac, N. (2011) An rpoB signature sequence provides unique resolution for the molecular typing of cyanobacteria. International Journal of Systematic and Evolutionary Microbiology 61: 170–83. https://doi.org/10.1099/ijs.0.019018-0Gan F., Shen, G. & Bryant D.A. (2015) Occurrence of Far-Red light Photoacclimation (FARLiP) in diverse cyanobacteria. Life 5 (1): 4–24. https://doi.org/10.3390/life5010004Gardner, N.L. (1927) New Myxophyceae from Porto-Rico. Memoirs of the New York Botanical Garden 7: 1–144.Garcia-Pichel, F., López-Cortés & Nübel, U. (2001) Phylogenetic and morphological diversity of cyanobacteria in soil desert crusts from the Colorado Plateau. Applied and Environmental Microbiology 67: 1902–1910. https://doi.org/10.1128/AEM.67.4.1902-1910.2001Geitler, L. (1932) Cyanophycceae. In: Rabenhorst, L. (Ed.) Kryptogamen-Flora von Deutschland, Österreich und der Schweiz. Ed. 2. Vol. 14. Akademische Verlagsgesellschaft, Leipzig, pp. 673–1196.Genuário, D.B., Lorenzi, A.S., Agujaro, L.F., Isaac, R.D.L., Azevedo, M.T.D.P., Neto, R.C. & Fiore, M.F. (2016) Cyanobacterial community and microcystin production in a recreational reservoir with constant Microcystis blooms. Hydrobiologia 779: 105–125. https://doi.org/10.1007/s10750-016-2802-yGomont, M. (1890) Essai de classification des Nostocacées homocystées. Journal de Botanique 4: 349–357.Gomont, M. (1892) Monographie des Oscillariées (Nostocacées Homocystées). Deuxième partie.-Lyngbyées. Annales des Sciences Naturelles, Botanique, Série 7 (16): 91–264.Gomont, M. (1899) Sur quelques Oscillariées nouvelles. Bulletin de la Société Botanique de France 46: 25–41, 1 pl.Gupta, R.S., Naushad, S. & Baker, S. (2015) Phylogenomic analyses and molecular signatures for the class Halobacteria and its two major clades: a proposal for division of the class Halobateria into an emended order Halobacteriales and two new orders, Haloferacales ord. nov. and Natrialbales ord. nov., containing the novel families Haloferacaceae fam. nov. and Natrialbaceae fam. nov. Systematic and Evolutionary Microbiology 65: 1050–1059. https://doi.org/10.1099/ijs.0.070136-0Hansgirg, A. (1885) Ein Beitrag zur Kenntniss von der Verbreitung der Chromatophoren und Zellkernen bei den Schizophyceen (Phycochromaceen). Berichte der deutsche botanischen Gesellschaft 3: 14–22.Hansgirg, A. (1887) Physiologische und algologische Studien. Berichtigungen, Prague, 187 pp.Hansgirg, A. (1892a) Über neue Süßwasser–und Meeres-Algen und Bacterien, mit Bemerkungen zur Sytematik dieser Phycophyten und über den Einfluss des Kichtes auf die Ortsbewegungen des Bacillus Pfeifferi nob. Sitzungsberichte der Königlichen Böhmischen Gesellschaft der Wissenschaften. Mathematisch-Naturwissenschaftliche Classe: 1–34.Hansgirg, A. (1892b) Prodomus der Algenflora von Böhmen. 2. Arch Naturwiss Landesdurchforsch Böhmen 8: 268.Hoffmann, L. (2005) Nomenclature of Cyanophyta/Cyanobacteria: roundtable on the unification of the nomenclature under the Botanical and Bacteriological Codes. Algological Studies 117: 13–29. https://doi.org/10.1127/1864-1318/2005/0117-0013Hunt, D. E., Klepac-Ceraj, V., Acinas, S.G., Gautier, C., Bertilsson, S. & Polz. M.F. (2006) Evaluation of 23S rRNA PCR primers for use in phylogenetic studies of bacterial diversity. Applied and Environmental Microbiology 72: 2221–2225. https://doi.org/10.1128/AEM.72.3.2221-2225.2006Jahodářová, E., Dvořák, P., Hašler, P. & Poulíčková, A. (2017) Revealing hidden diversity among tropical cyanobacteria: the new genus Onodrimia (Synechococcales, Cyanobacteria) described using the polyphasic approach. Phytotaxa 329 (1): 28–40. https://doi.org/10.11646/phytotaxa.326.1.2Jahodářová, E., Dvořák, P., Hašler, P., Holušová, K. & Poulíčková, A. (2017) Elainella gen. nov.: a new tropical cyanobacterium characterized using a complex genomic approach. European Journal of Phycology, pp. 1–13. https://doi.org/10.1080/09670262.2017.1362591Jao, C.C. (1948) The marine Myxophyceae in the vicinity of Friday Harbor, Washington. Botanical Bulletin of Academia Sinica 2: 161–177.Jancusova, M., Kovacik, L., Pereira, A.B., Dusinsky, R. & Wilmottee A. (2016) Polyphasic characterization of 10 selected ecological relevant filamentous cyanobacterial strains from the South Shetland Islands, Maritime Antartica. FEMS Microbiology Ecology 92 7. https://doi.org/10.1093/femsec/fiw100Johansen, J.R. & Casamatta, D.A. (2005) Recognizing cyanobacterial diversity through adoption of a new species paradigm. Algological Studies 117: 71–93. https://doi.org/10.1127/1864-1318/2005/0117-0071Johansen, J.R., Olsen, C.E., Lowe, R.L., Fučíková, K. & Casamatta, D.A. (2008) Leptolyngbya species from selected seep walls in the Great Smoky Mountains National Park. Algological Studies 126: 21–36. https://doi.org/10.1127/1864-1318/2008/0126-0021Johansen, J.R., Kovacik, L., Casamatta, D.A., Fučiková, K. & Kaštovský, J. (2011) Utility of 16S-23S ITS sequence and secondary structure for recognition of intrageneric and intergeneric limits within cyanobacterial taxa: Leptolyngbya corticola sp. nov. (Pseudanabanaceae, Cyanobacteria). Nova Hedwigia 92: 283–302. https://doi.org/10.1127/0029-5035/2011/0092-0283Johansen, J.R., Bohunická, M., Lukešová, A., Hrčková, K., Vaccarino, M.A. & Chesarino, N.M. (2014) Morphological and molecular characterization within 26 strains of the genus Cylindrospermum (Nostocaceae, Cyanobacteria), with descriptions of three new species. Journal of Phycology 50: 187–202. https://doi.org/10.1111/jpy.12150Kaštovský, J., Berrendero-Gomez, E., Hladil, J. & Johansen, J.R. (2014) Cyanocohniella calida gen. et sp. nov. (Cyanobacteria: Aphanizomenonaceae) a new cyanobacterium from the thermal springs from Karlovy Vary, Crezch Republic. Phytotaxa 181 (5): 279–292. https://doi.org/10.11646/phytotaxa.181.5.3Kisselev (1935) Über das Phytoplankton einiger Seen des Jakutzkschen Gebietes (Autonome Jakutzk Republik) in Explorations des lacs de I’URSS, VIII. Leningrad Institut Hydrologique, 1908 pp.Komárek, J. (1975) Geitleribactron eine neue, Chamaesiphon-ähnliche Blaualgengattung. Plant Systematics and Evolution 123: 263–281. https://doi.org/10.1007/BF00987060Komárek, J. & Lukavský, J. (1988) Arthronema, a new cyanophyte genus from Afro-Asian deserts. Archiv für Hydrobiologie, Supplement 50–53: 249–267.Komárek, J. & Kling, H. (1991) Variation in six planktonic cyanophyte genera in Lake Victoria (East Africa). Archiv für Hydrobiologie, Supplement 61: 21–45.Komárek, O. & Komárek, J. (2001) Contribution to the taxonomy and ecology of cryosestic algae in the summer season 1995–96 at King George Island S. Shetland Islands. Nova Hedwigia 123:121–140.Komárek, J. & Anagnostidis, K. (2005) Cyanoprokaryota II. In: Büdel, B., Krienitz, L., Gärtner, G. & Schagerl, M. (Eds.) Süsswasserflora von Mittleuropa 19/2. Elsevier/Spektrum, München, 759 pp.Komárek, J., Kaštovský, Ventura, S., Turicchia, S. & Šmarda, J. (2009) The cyanobacterial genus Phormidesmis. Algological Studies 129: 41–59. https://doi.org/10.1127/1864-1318/2009/0129-0041Komárek, J., Kaštovský, J., Mareš, J. & Johansen, J.R. (2014) Taxonomic classification of cyanoprokaryotes (cyanobacterial genera) 2014, using a polyphasic approach. Preslia 86: 295–335.Komárek, J. (2006) Cyanobacterial taxonomy: current problems and prospects for the integration of traditional and molecular approach. Algae 21: 349–375. https://doi.org/10.4490/ALGAE.2006.21.4.349Komárková-Legnerová, J. & Cronberg, G. (1992) New and recombined filamentous Cyanophytes from lakes in South Scania, Sweden. Archiv für Hydrobiologie, Supplement 67: 21–31.Krautová, M. (2008) Cyanobacteria of Wet Walls, Seep Walls and Hanging Gardens in Grand Staircase-Escalante National Monument, Utah (Master’s thesis). Retreived from John Carroll University, 166 pp.Kützing, T.F. (1843) Phycologia generalis order Anatomie, Phycologie und Systemkunde der Tange. Brockhaus, Leipzig, 458 pp.Larkin, M.A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan, P.A., McWilliam, H., Valentin, F., Wallace, I.M., Wilm, A., Lopez, R., Thompson, J.D., Gibson T.J. & Higgins, D.G. (2007) ClustalW and ClustalX version 2. Bioinformatics 23: 2947–2948. https://doi.org/10.1093/bioinformatics/btm404Lauterborn, H. (1915) Die sapropelische Lebewelt. Ein Beitrag zur biologie des Faulschlamms natürlicher Gewässer. Verhandlungen des Naturhistorischen-Medizinischen Vereins zu Heidelberg 13: 395–481.Lemmermann, E. (1898a) Der grosse Waterneverstorfer Binnensee. Eine biologische Studie. Forschungsberichte aus der Biologischen Station zu Plön 6: 166–205.Lemmermann, E. (1898b) Beitrage zur Kenntniss der Planktonalgen. II. Beschreibung neuer Formen. Botanisches Centralblatt 76: 150–156.Lemmermann, E. (1899) Plankton-algen. Ergebnisse einer Reise nach dem Pacific. Abhandlungen herausgegeben vom naturwissenschaftlichen Verein zu Bremen 16: 313–398.Lemmermann, E. (1904) Das Plankton schwedischer Gewässer. Arkiv för Botanik 2: 1–209.Lemmermann, E. (1910) Algen I (Schizophyceen, Flagellaten, Peridineen), Dritter Band. In: Kryptogamenflora der Mark Brandenburg und angrenzender Gebiete Herausgegeben von dem Botanishcen Verein der Provinz Brandenburg. Verlag von Gebrüder Borntraeger. Leipzig, pp. 497–712. https://doi.org/10.5962/bhl.title.4953Li Z. & Brand J. (2007) Leptolyngbya nodulosa sp. nov. (Oscillatoriaceae), a subtropical marine cyanobacterium that produces a unique multicellular structure. Phycologia 46 (4): 396–401. https://doi.org/10.2216/06-89.1Li, Y. & Chen, M. (2015) Novel chlorophylls and new directions in photosynthesis research. Functional Plant Biology 42: 493–501. https://doi.org/10.1071/FP14350Li, X. & Li, R. (2016) Limnolyngbya circumcreta gen & comb. nov. (Synechococcales, Cyanobacteria) with three geographical (provincial) genoptypes in China. Phycologia 55: 478–491. https://doi.org/10.2216/15-149.1Limanowska, H. (1912) Die Algenflora der Limmat vom Zürichsee bis unterhalb des Wasserwerkes. Archiv fur Hydrobiologie 7: 364.Ludwig, W. & Schleifer, K. (1994) Bacterial phylogeny based on 16S and 23S rRNA sequence analysis. FEMS Microbiological Reviews 15: 155–173. https://doi.org/10.1111/j.1574-6976.1994.tb00132.xMareš, J. (2017) Multilocus and SSU rRNA gene phylogenetic analyses of available cyanobacterial genomes, and their relation to the current taxonomic system. Hydrobiologia 811: 19–34. https://doi.org/10.1007/s10750-017-3373-2McNeill, J., Barrie, F.R., Buck, W.R., Demoulin, V., Greuter, W., Hawksworth, D.L., Heredeen, P.S., Knapp, S., Marhold, K., Prado, J., Prud’homme van Reine, W.F., Smith, G.F., Wiersema, J.H. & Turland, N.J. (2012) International Code of Nomenclature for algae, fungi, and plants (Melbourne Code). Regnum vegetabile 154. Koeltz Scientific Books, Kapellenbergst, 208 pp.Meffert, M.E. (1971) Cultivation and growth of two planktonic Oscillatoria species. Mitteilungen der Internationalen Vereinigung für Theoretische und Angewandte Limnologie 19: 189–205. https://doi.org/10.1080/05384680.1971.11903930Meffert, M.E. (1988) Limnothrix Meffert nov. gen. The unsheathed planktic cyanophycean filaments with polar and central gas vacuoles. Algological Studies/Archiv für Hydrobiologie, Supplement Volumes: 269–276.Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010, November) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. Proceedings of the Gateway Computing Environments Workshop (GCE), 2010: 1–8. https://doi.org/10.1109/GCE.2010.5676129Miscoe, L.H., Johansen, J.R., Kociolek, J.P., Lowe, R.L., Vaccarino, M.A., Pietrasiak, N. & Sherwood, A.R. (2016) Novel cyanobacteria from caves on Kauai, Hawaii. Bibliotheca Phycologica 120: 75–152.Mishler, B.D. & Theriot, E.C. (2000) The phylogenetic species concept (sensu mishler and theriot): monophyly, apomorphy, and phylogenetic species concepts. In: Wheeler, Q. & Meier, R. (Eds.) Species Concepts and Phylogenetic Theory: A Debate. Columbia University Press, New York, pp. 44–54.Moro, I., Rascio, N., Rocca, N.L, Sciuto, K., Albertano, P., Bruno, L. & Andreoli, C. (2010) Polyphasic characterization of a thermo-tolerant filamentous cyanobacterium isolated from the Euganean thermal muds (Padua, Italy). European Journal of Phycology 45: 143–154. https://doi.org/10.1080/09670260903564391Mühlsteinová, R., Johansen, J.R., Pietrasiak, N., Martin, M.P., Osorio-Santos, K. & Warren, S.D. (2014) Polyphasic characterization of Trichocoleus desertorum sp. nov. (Pseudanabanales, Cyanobacteria) from desert soils and phylogenetic placement of the genus Trichocoleus. Phytotaxa 163 (5): 241–261. https://doi.org/10.11646/phytotaxa.163.5.1Naushad, S., Adeolu, M., Goel, N., Khadka, B., Al-Dahwi, A. & Gupta, R.S. (2015) Phylogenomic and molecular demarcation of the core members of the polyphyletic Pasteurellaceae genera Actinobacillus, Haemophilus, and Pasteurella. International Journal of Genomics 2015: 15. https://doi.org/10.1155/2015/198560Nübel, U., Garcia-Pichel, F. & Muyzer, G. (1997) PCR primers to amplify 16S rRNA genes from cyanobacteria. Applied and Environmental Microbiology 63: 3327–3332.Nübel, U., Garcia-Pichel, F. & Muyzer, G. (2000) The halotolerance and phylogeny of cyanobacteria with tightly coiled trichomes (Spirulina Turpin) and the description of Halospirulina tapeticola gen. nov., sp. nov. International Journal of Stystematics and Evolutionary Microbiology 50: 1265–1277. https://doi.org/10.1099/00207713-50-3-1265Osorio-Santos, K., Pietrasiak, N., Bohunická, Miscoe, L.H., Kováčik L., Martin, M.P. & Johansen J.R. (2014) Seven new species of Oculatella (Pseudanabaenales, Cyanobacteria): taxonomically recognizing cryptic diversification. European Journal of Phycology 49: 450–470. https://doi.org/10.1080/09670262.2014.976843Paul, R., Jinkerson, R.E., Bss, K., Steel J., Mohr R., Hess, W.R., Chen, M. & Fromme, P. (2014) Draft genome sequence of the Filamentous cyanobacterium Leptolyngbya sp. strain Heron Island J, exhibiting chromatic acclimation. Genomic Announcements 2 (1): 1–2. https://doi.org/10.1128/genomeA.01166-13Pekerson III, R.B., Johansen, J.R, Kovácik, L., Brand, J., Kaštovský, J. & Casamatta, D.A. (2011) A unique Pseudanabaenalean (Cyanobacteria) genus Nodosilinea gen. nov. based on morphological and molecular data. Journal of Phycology 47: 1397–1412. https://doi.org/10.1111/j.1529-8817.2011.01077.xPietrasiak, N., Mühlsteinová, R., Siegesmund, M.A. & Johansen, J.R. (2014) Phylogenetic placement of Symplocastrum (Phormidiaceae, Cyanophyceae) with a new combination S. californicum and two new species: S. fletchnerae and S. torsivum. Phycologia 53: 529–541. https://doi.org/10.2216/14-029.1Playfair, G.I. (1915) Freshwater algae of the Lismore district: with an appendix on the algal fungi and schizomycetes. Proceedings of the Linnean Society of New South Wales 40: 310–362. https://doi.org/10.5962/bhl.part.18876Rabenhorst, L. (1865) Flora europaea algarum aquae dulcis et submarinae. Sectio II. Algas phycochromaceas complectens. Lipsiae: Apud Eduardum Kummerum, Leipzig, pp. 1–139.Řeháková, K., Johansen, J.R., Casamatta, D.A., Xuesong, L. & Vincent, J. (2007) Morphological and molecular characterization of selected desert soil cyanobacteria: three species new to science including Mojavia pulchra gen. et. sp. nov. Phycologia 46: 481–502. https://doi.org/10.2216/06-92.1Řeháková, K., Johansen, J.R., Bowen, M.B., Martin, M.P. & Sheil, C.A. (2014) Variation in secondary structure of the 16S rRNA molecule in cyanobacteria with implications for phylogenetic analysis. Fottea 14: 161–178. https://doi.org/10.5507/fot.2014.013Rijk, P.D., Peer, Y.V.D., Broeck, I.V.D. & Wachter, R.D. (1995) Evolution according to large ribosomal subunit RNA. Journal of Molecular Evolution 41: 366–375. https://doi.org/10.1007/BF01215184Rippka, R., Waterbury, J. & Cohen-Bazire, G. (1974) A cyanobacterium which lacks thylakoids. Archives for Microbiology 100: 419–436. https://doi.org/10.1007/BF00446333Rosselló-Mora, R. & Amann, R. (2001) The species concept for prokaryotes. FEMS Microbiology Reviews 25: 39–67. https://doi.org/10.1111/j.1574-6976.2001.tb00571.xSabbe, K., Hodgson, D.A., Verleyen, E., Taton, A., Wilmotte, A., Vanhoutte, K. & Vyverman, W. (2004) Salinity, depth and the structure and composition of microbial mats in continental Antartic lakes. Freshwater Biology 49: 296–319. https://doi.org/10.1111/j.1365-2427.2004.01186.xSangal, V., Goodfellow, M., Jones, A.L., Schwalbe, E.C., Blom, J., Hoskisson, P.A. & Sutcliffe, I.C. (2016) Next-generation systematics: An innovative approach to resolve the structure of complex prokaryotic taxa. Scientific Reports 6: 383–392. https://doi.org/10.1038/srep38392Sauvageau, C. (1892) Sur les algues d’eau douce recoltées en Algérie pendant le session de la Societé Botanique en 1892. Bulletin de la Société Botanique de France 39: CIV–CXXVIII. https://doi.org/10.1080/00378941.1892.10828721Sawana, A., Adeolu, M. & Gupta, R.S. (2014) Molecular signatures and phylogenomic analysis of the genus Burkholderia: proposal for division of this genus into the emended genus Burkholderia containing pathogenic organisms and a new genus Paraburkholderai gen. nov. harboring environmental species. Frontiers in Genetics 5: 429. https://doi.org/10.3389/fgene.2014.00429Sciuto, K., Rascio, N., Andreoli, C. & Moro, I. (2011) Characterization of ITD-01, a cyanobacterium isolated from the Ischia Thermal District (Napales, Italy). Fottea 11 (1): 31–39. https://doi.org/10.5507/fot.2011.005Sciuto, K. & Moro, I. (2016) Detection of the new cosmopolitan genus Thermoleptolyngbya (Cyanobacteria, Leptolyngbyaceae) using the 16S rRNA gene and 16S-23S ITS region. Molecular Phylogenetics and Evolution 105: 15–35 https://doi.org/10.1016/j.ympev.2016.08.010Sciuto, K., Moschin, E. & Moro, I. (2017) Cryptic cyanobacterial diversity in the Giant Cave (Trieste, Italy): the new genus Timaviella (Leptolyngbyaceae). Cryptogamie, Algologie 38: 285–323. https://doi.org/10.7872/crya/v38.iss4.2017.285Schwabe, G.H. (1944) Umraumfremde Quellen. Mitteilungen der deutschen Gesellschaft für Natur-und Völkerkunde Ostasiens Suppl. 21: 1–300.Hentschke, G.S., Johansen, J.R., Pietrasiak, N., Fiore, M.D.F., Rigonato, J., Sant’Anna, C.L. & Komárek J. (2016) Phylogenetic placement of Dapistostemon gen. nov. and Streptostemon, two tropical heterocytous genera (Cyanobacteria). Phytotaxa 245 (2): 129–143. https://doi.org/10.11646/phytotaxa.245.2.4Seo, P.S. & Yokota, A. (2003) The relationships of cyanobacteria inferred from 16S rrNA, gyrB, rpoC1 and rpoD1 gene sequences. Journal of General and Applied Microbiology 49: 191–203. https://doi.org/10.2323/jgam.49.191Shimura, Y., Hirose, Y., Misawa, N., Osana, Y., Katoh, H., Yamaguchi, H. & Kawachi, M. (2015) Comparison of the terrestrial cyanobacterium Leptolyngbya sp. NIES-2104 and the freshwater Leptolyngbya boryana PCC 6306 genomes. DNA Research 22 (6): 403–412. https://doi.org/10.1093/dnares/dsv022Skuja, H. (1939) Beitrag zur Algenflora Lettlands. II. Acta horti botanici Universitatis Latviensis 11–12: 41–51.Song, G., Jiang, Y. & Li, R. (2015) Scytolyngbya timoleontis gen et sp. nov. (Leptolyngbyaceae, Cyanobacteria): a novel false branching Cyanobacteria from China. Phytotaxa 224 (1): 72–84. https://doi.org/10.11646/phytotaxa.224.1.5Starmach, K. (1959 ‘1960’) Homeothrix janthina (Born. et Flah.) comb. nova mihi (= Amphithrix janthina Born. et Flah.) oraz sinice towarzyszce. Acta Hydrobiologica [Kraków] 1: 149–164.Shen, G., Gan, F. & Bryant, D.A. (2016) The siderophillic cyanobacterium Leptolyngbya sp. strain JSC-1 acclimates to iron starvation by expressing multiple isiA-family genes. Photosynthetic Research 128 (3): 325–340. https://doi.org/10.1007/s11120-016-0257-7Sherwood, A.R. & Presting, G.G. (2007) Universal primers amplify a 23S rDNA plastid marker in eukaryotic algae and cyanobacteria. Journal of Phycology 43: 605–608. https://doi.org/10.1111/j.1529-8817.2007.00341.xSherwood, A.R., Carlile, A.M., Vaccarino, M.A. & Johansen, J.R. (2015) Characterization of Hawaiian freshwater and terrestrial cyanobacterial reveals high diversity and numerous putative endemics. Phycological Research 63: 85–92. https://doi.org/10.1111/pre.12080Taton, A., Grubisic, S., Ertz, D., Hodson, D.A., Piccardi, R., Biondi, N., Tredici, M.R., Mainini, M., Daniele, L., Marinelli, F. & Wilmotte, A. (2006) Polyphasic study of Antartic cyanobacterial strains. Journal of Phycology 42: 1257–1270. https://doi.org/10.1111/j.1529-8817.2006.00278.xTaton, A., Wilmotte, A., Smarda, J., Elster, J. & Komárek, J. (2011) Plectolyngbya hodgsonii: a novel filamentous cyanobacterium from Antarctic lakes. Polar Biology 34: 181–191. https://doi.org/10.1007/s00300-010-0868-yThomas, J.C. & Gonzalves, E.A. (1965) Thermal algae of Western India. I. Algae of hot springs at Akloli and Ganeshpuri. Hydrobiologia 25: 334. https://doi.org/10.1007/BF00142253Tomitani, A., Knoll, A.H., Cavanaugh, C.M. & Ohno, T. (2006) The evolutionary diversification of cyanobacteria: Molecular-phylogenetic and paleontological perspectives. Proceedings of the National Academy of Sciences of the United States of America 103: 5442–5447. https://doi.org/10.1073/pnas.0600999103Turicchia, S., Ventura, S., Komárková, J. & Komárek, J. (2009) Taxonomic evaluation of cyanobacterial microflora from alkaline marshes of northern Belize. 2. Diversity of oscillatorialean genera. Nova Hedwigia 89: 165–200. https://doi.org/10.1127/0029-5035/2009/0089-0165Umezaki, I. (1962) Yonedaella nom. nov. Taxon 11: 204.Umezaki, I. & Watanabe, M. (1994) Enumeration of the Cyanophyta (blue-green algae) of Japan. 1. Chroococcales and Oscillatoriales. Japanese Journal of Phycology 42: 175–219.Urbach, E., Robertson, D.L. & Chisholm S.W. (1992) Multiple evolutionary origins of prochlorophytes within the cyanobacterial radiation. Nature 355: 267–270. https://doi.org/10.1038/355267a0Vaz, M.G.M.V, Genuáro, D.B., Andreote, A.P.D., Malone, C.F.S., Sant’Anna C.L., Barbiero, L. & Fiore, M.F. (2015) Pantanalinema gen. nov. and Alkalinema gen. nov.: novel pseudanabaenacean genera (Cyanobacteria) isolated from saline-alkaline lakes. International Journal of Systematics and Evolutionary Microbiology 65: 298–308. https://doi.org/10.1099/ijs.0.070110-0West, G.S. (1907) Report of the freshwater algae incl. phytoplankton of the Third Tanganyika Expedition. Botanical Journal of the Linnean Society 38: 81–194. https://doi.org/10.1111/j.1095-8339.1907.tb00848.xWilmotte, A., Auwera, G.V.D. & Wachter, R.D. (1993) Structure of the 16S rRNA of the thermophilic cyanobacterium Chlorogloeopsis HTF (‘Mastigocladus laminosus hTF’) strain PCC7518, and phylogenetic analysis. Federation of European Biochemical Societies 317: 96–100. <a href="https://doi.org/10.1016/0014-5793(93)81499-P">https://doi.org/10.1016/0014-5793(93)81499-P</a>Wilmotte, A. (2004) Molecular evolution and taxonomy of the cyanobacteria. In: Bryant, D.A. (Ed.) Advances in Photosynthesis and Respiration I. Springer, Berlin, pp. 1–25. https://doi.org/10.1007/0-306-48205-3_1Wilson, K.M., Schembri, M.A., Baker, P.D. & Saint, C.P. (2000) Molecular characterization of the toxic cyanobacterium Cylindrospermopsis raciborskii and design of a species-specific PCR. Applied and Environmental Microbiology 66: 332–338. https://doi.org/10.1128/AEM.66.1.332-338.2000Woronichin, N.N. (1930) Algen des Polar-und des Nord-Urals. Travaux de la Société de Naturalistes de Leningrad 60: 1–77.Yarza, P., Yilmaz, P., Pruesse, E., Glöckner, Ludwig, W., Schleifer, K.H., Witman, W.B., Euzéby, J., Amann, R. & Rosselló-Móra. (2014) Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences. Nature Reviews Microbiology 12: 635–645. https://doi.org/10.1038/nrmicro3330Zammit, G., Billi, D. & Albertano, P. (2012) The subaerophytic cyanobacterium Oculatella subterranea (Oscillatoriales, Cyanophyceae) gen. et sp. nov.: a cytomorphological and molecular description. European Journal of Phycology 47: 341–354. https://doi.org/10.1080/09670262.2012.717106Zuker, M. (2003) Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Research 31: 3406–3415. https://doi.org/10.1093/nar/gkg595Zopf, W.F. (1882) Zur Morphologie der Spaltpflanzen (Spaltpilze und Spaltalgen). Verlag von Veit & Comp, Leipzig, pp. 1–74.