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Issue: Vol. 722 No. 3: 15 Oct. 2025
Type: Article
Published: 2025-10-15
DOI: 10.11646/phytotaxa.722.3.1
Page range: 207-221
Abstract views: 235
PDF downloaded: 4

Cytospora guizhouensis sp. nov., an endophytic fungus from Rosa roxburghii in Guizhou, China

School of Food Engineering, Moutai Institute, Renhuai, Guizhou Province, 564502, P.R. China; Department of Plant Pathology, College of Agriculture, Guizhou University, Guiyang, Guizhou Province, 550025, P.R. China
Department of Plant Pathology, College of Agriculture, Guizhou University, Guiyang, Guizhou Province, 550025, P.R. China
1 new species Cytosporaceae morphology multigene phylogeny taxonomy Fungi

Abstract

Rosa roxburghii, an economically significant medicinal and edible plant endemic to Southwestern China, particularly in Guizhou Province. In a survey of microfungi associated with R. roxburghii in China, three endophytic taxa were isolated from R. roxburghii. Phylogenetic analyses of concatenated LSU, ITS, rpb2, act, tef1, and tub2 sequence, combined with morphological characteristics, supported their classification as a novel species, Cytospora guizhouensis sp. nov. This taxonomic clarification establishes critical baseline data for further exploration of this endophytic resource's biotechnological potential.

References

  1. Adams, G.C., Surve-Iyer, R.S. & Iezzoni, A.F. (2002) Ribosomal DNA sequence divergence and group I introns within the Leucostoma species L. cinctum, L. persoonii and L. parapersoonii sp. nov., ascomycetes that cause Cytospora canker of fruit trees. Mycologia 94 (6): 947–967. https://doi.org/10.1080/15572536.2003.11833153
  2. Adams, G.C., Wingfield, M.J., Common, R. & Roux, J. (2005) Phylogenetic relationships and morphology of Cytospora species and related teleomorphs (Ascomycota, Diaporthales, Valsaceae) from Eucalyptus. Studies in Mycology 52: 1–144.
  3. Capella-Gutierrez, S., Silla-Martinez, J.M. & Gabaldon, T. (2009) trimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses. Bioinformatics 25: 1972–1973. https://doi.org/10.1093/bioinformatics/btp348
  4. Carbone, I. & Kohn, L.M. (1999) A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91 (3): 553–556. https://doi.org/10.1080/00275514.1999.12061051
  5. Crous, P.W., Catcheside, D.E.A., Catcheside, P.S., Alfenas, A.C., Alfenas, R.F., Barreto, R.W., Lebel, T., Balashov, S., Broadbridge, J., Jurjević, Ž., De la Peña-Lastra, S., Hoffmann, R., Mateos, A., Riebesehl, J., Shivas, R.G., Soliz Santander, F.F., Tan, Y.P., Altés, A., Bandini, D., Carriconde, F., Cazabonne, J., Czachura, P., Gryta, H., Eyssartier, G., Larsson, E., Pereira, O.L., Rigueiro-Rodríguez, A., Wingfield, M.J., Ahmad, W., Bibi, S., Denman, S., Esteve-Raventós, F., Hussain, S., Illescas, T., Luangsa-Ard, J.J., Möller, L., Mombert, A., Noisripoom, W., Olariaga, I., Pancorbo, F., Paz, A., Piątek, M., Polman-Short, C., Suárez, E., Afshan, N.S., Ali, H., Arzanlou, M., Ayer, F., Barratt, J., Bellanger, J.M., Bidaud, A., Bishop-Hurley, S.L., Bohm, M., Bose, T., Campo, E., Chau, N.B., Çolak, Ö.F., Cordeiro, T.R.L., Cruz, M.O., Custódio, F.A., Couceiro, A., Darmostuk, V., Dearnaley, J.D.W., de Azevedo Santiago, A., de Freitas, L.W.S., Yáñez-Morales, M.J., Domnauer, C., Dentinger, B., Dhileepan, K., De Souza, J.T., Dovana, F., Eberhardt, U., Eisvand, P., Erhard, A., Fachada, V., García-Martín, A., Groenewald, M., Hammerbacher, A., Harms, K., Haroon, S., Haqnawaz, M., Henriques, S., Hernández, A.J., Jacobus, L.M., Jaen-Contreras, D., Jangsantear, P., Kaygusuz, O., Knoppersen, R., Kumar, T.K.A., Lynch, M.J., Mahiques, R., Maraia, G.L., Marbach, P.A.S., Mehrabi-Koushki, M., Miller, P.R., Mongkolsamrit, S., Moreau, P.A., Oberlies, N.H., Oliveira, J.A., Orlovich, D., Pérez-Méndez, A.S., Pinto, A., Raja, H.A., Ramírez, G.H., Raphael, B., Rodrigues, A., Rodrigues, H., Ramos, D.O., Safi, A., Sarwar, S., Saar, I., Sánchez, R.M., Santana, J.S., Scrace, J., Sales, L.S., Silva, L.N.P., Stryjak-Bogacka, M., Tacconi, A., Thanh, V.N., Thomas, A., Thuy, N.T., Toome, M., Valdez-Carrazco, J.M., van Vuuren, N.I., Vasey, J., Vauras, J., Vila-Viçosa, C., Villarreal, M., Visagie, C.M., Vizzini, A., Whiteside, E.J. & Groenewald, J.Z. (2025) Fungal Planet description sheets: 1781–1866. Persoonia 54: 327–587. https://doi.org/10.3114/persoonia.2025.54.10
  6. Ehrenberg, C.G. (1818) Sylvae Mycologicae Berolinenses. Formis Theophili Bruschcke, Berlin, Germany.
  7. Fan, X., Hyde, K.D., Liu, M., Liang, Y. & Tian, C. (2015) Cytospora species associated with walnut canker disease in China, with description of a new species C. gigalocus. Fungal Biology 119 (5): 310–319. https://doi.org/10.1016/j.funbio.2014.12.011
  8. Fan, X.L., Bezerra, J.D.P., Tian, C.M. & Crous, P.W. (2020) Cytospora (Diaporthales) in China. Persoonia 45 (1): 1–45. https://doi.org/10.3767/persoonia.2020.45.01
  9. Fan, L., Li, Y., Wang, X., Leng, F., Li, S., Zhu, N., Chen, K. & Wang, Y. (2023) Culturable endophytic fungi community structure isolated from Codonopsis pilosula roots and effect of season and geographic location on their structures. BMC Microbiology 23 (1): 132. https://doi.org/10.1186/s12866-023-02848-3
  10. Glass, N.L. & Donaldson, G.C. (1995) Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Applied and Environmental Microbiology 61 (4): 1323–1330. https://doi.org/10.1128/aem.61.4.1323-1330.1995
  11. Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98. https://doi.org/10.1021/bk-1999-0734.ch008
  12. Huson, D.H. & Bryant, D. (2006) Application of phylogenetic networks in evolutionary studies. Molecular Biology and Evolution 23: 254–267. https://doi.org/10.1093/molbev/msj030
  13. Jain, A., Sarsaiya, S., Gong, Q., Wu, Q. & Shi, J. (2024) Chemical diversity, traditional uses, and bioactivities of Rosa roxburghii Tratt: A comprehensive review. Pharmacology & Therapeutics 259: 108657. https://doi.org/10.1016/j.pharmthera.2024.108657
  14. Jia, A., Chen, B., Lu, H., Xing, Y., Li, B. & Fan, X. (2024) Multigene phylogeny and morphology reveal three new species of Cytospora isolated from diseased plant branches in Fengtai District, Beijing, China. MycoKeys 101: 163–189. https://doi.org/10.3897/mycokeys.101.116272
  15. Kalyaanamoorthy, S., Minh, B.Q., Wong, T.K., Von Haeseler, A. & Jermiin, L.S. (2017) ModelFinder: fast model selection for accurate phylogenetic estimates. Nature Methods 14 (6): 587–589. https://doi.org/10.1038/nmeth.4285
  16. Kianfé, B.Y., Tchamgoue, J., Narmani, A., Teponno, R.B., Njouonkou, A.L., Stadler, M. & Fogue Kouam, S. (2023) Bioactive secondary metabolites from fungi of the genus Cytospora Ehrenb.(Ascomycota). Molecules 28 (7): 3120. https://doi.org/10.3390/molecules28073120
  17. Lawrence, D.P., Holland, L.A., Nouri, M.T., Travadon, R., Abramians, A., Michailides, T.J. & Trouillas, F.P. (2018) Molecular phylogeny of Cytospora species associated with canker diseases of fruit and nut crops in California, with the descriptions of ten new species and one new combination. IMA Fungus 9: 333–369. https://doi.org/10.5598/imafungus.2018.09.02.07
  18. Larsson, A. (2014) AliView: a fast and lightweight alignment viewer and editor for large datasets. Bioinformatics 30: 3276–3278. https://doi.org/10.1093/bioinformatics/btu531
  19. Li, J.T., Li, J.R. & Jiang, N. (2024) Identification of Cytospora species isolated from branch canker diseases of woody plants in Tibet, China. Forests 15 (1): 121. https://doi.org/10.3390/f15010121
  20. Li, J., Luo, Y., Lu, M., Wu, X. & An, H. (2022) The pathogen of top rot disease in Rosa roxburghii and its effective control fungicides. Horticulturae 8 (11): 1036. https://doi.org/10.3390/horticulturae8111036
  21. Lin, L., Pan, M., Tian, C. & Fan, X. (2022) Fungal richness of Cytospora species associated with willow canker disease in China. Journal of Fungi 8 (4): 377. https://doi.org/10.3390/jof8040377
  22. Lin, L., Pan, M., Bezerra, J.D., Tian, C. & Fan, X. (2023) Re-evaluation of the fungal diversity and pathogenicity of Cytospora species from Populus in China. Plant Disease 107 (1): 83–96. https://doi.org/10.1094/PDIS-02-22-0260-RE
  23. Lin, L., Fan, X.L., Groenewald, J.Z., Jami, F., Wingfield, M.J., Voglmayr, H., Jaklitsch, W., Castlebury, L.A., Tian, C.M. & Crous, P.W. (2024) Cytospora: an important genus of canker pathogens. Studies in Mycology 109: 323–401. https://doi.org/10.3114/sim.2024.109.05
  24. Liu, Y.J., Whelen, S. & Hall, B.D. (1999) Phylogenetic relationships among ascomycetes: evidence from an RNA polymerase II subunit. Molecular Biology and Evolution 16: 1799–1808. https://doi.org/10.1093/oxfordjournals.molbev.a026092
  25. Liu, H.X., Tan, H.B., Chen, K., Zhao, L.Y., Chen, Y.C., Li, S.N., Li, H.H. & Zhang, W.M. (2019) Cytosporins A–D, novel benzophenone derivatives from the endophytic fungus Cytospora rhizophorae A761. Organic & Biomolecular Chemistry 17 (9): 2346–2350. https://doi.org/10.1039/C8OB03223H
  26. Ma, W., Shao, Z., Chen, Y., Li, S., Liu, H., Zhang, W. & Gao, X. (2024) Cytospotones AD, four new polyketones from the endophytic fungus Cytospora sp. A879. Fitoterapia 173: 105751. https://doi.org/10.1016/j.fitote.2023.105751
  27. Minh, B.Q., Nguyen, M.A.T. & von Haeseler, A. (2013) Ultrafast approximation for phylogenetic bootstrap. Molecular Biology and Evolution 30: 1188–1195. https://doi.org/10.1093/molbev/mst024
  28. Monkai, J., Tibpromma, S., Manowong, A., Mapook, A., Norphanphoun, C., Hyde, K.D. & Promputtha, I. (2021) Discovery of three novel Cytospora species in Thailand and their antagonistic potential. Diversity 13 (10): 488. https://doi.org/10.3390/d13100488
  29. Nguyen, L.T., Schmidt, H.A., von Haeseler, A. & Minh, B.Q. (2014) IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular Biology and Evolution 32: 268–274. https://doi.org/10.1093/molbev/msu300
  30. Norphanphoun, C., Raspé, O., Jeewon, R. & Hyde, K.D. (2018) Morphological and phylogenetic characterisation of novel Cytospora species associated with mangroves. MycoKeys 38: 93–120. https://doi.org/10.3897/mycokeys.38.28011
  31. Pan, M., Zhu, H., Bonthond, G., Tian, C. & Fan, X. (2020) High diversity of Cytospora associated with canker and dieback of Rosaceae in China, with 10 new species described. Frontiers in Plant Science 11: 690. https://doi.org/10.3389/fpls.2020.00690
  32. Pan, M., Zhu, H., Tian, C., Huang, M. & Fan, X. (2021) Assessment of Cytospora isolates from conifer cankers in China, with the descriptions of four new Cytospora species. Frontiers in Plant Science 12: 636460. https://doi.org/10.3389/fpls.2021.636460
  33. Quaedvlieg, W., Binder, M., Groenewald, J.Z., Summerell, B.A., Carnegie, A.J., Burgess, T.I. & Crous, P.W. (2014) Introducing the consolidated species concept to resolve species in the Teratosphaeriaceae. Persoonia 33 (1): 1–40. https://doi.org/10.3767/003158514X681981
  34. Razaghi, P., Raza, M., Han, S.L., Ma, Z.Y., Cai, L., Zhao, P., Chen, Q., Phurbu, D. & Liu, F. (2024) Sporocadaceae revisited. Studies in Mycology 109: 155–272. https://doi.org/10.3114/sim.2024.109.03
  35. Ronquist, F., Teslenko, M., Van Der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61 (3): 539–542. https://doi.org/10.1093/sysbio/sys029
  36. Shenoy, B.D., Jeewon, R. & Hyde, K.D. (2007) Impact of DNA sequence-data on the taxonomy of anamorphic fungi. Fungal Diversity 26: 1–54.
  37. Spielman, L.J. (1983) Taxonomy and biology of Valsa species on hardwoods in North America, with special reference to species on maples. [PhD dissertation]. Cornell University.
  38. Sun, Y.R., Hyde, K.D., Liu, N.G., Jayawardena, R.S., Wijayawardene, N.N., Ma, J., Zhang, Q., Al-Otibi, F. & Wang, Y. (2025) Micro-fungi in southern China and northern Thailand: emphasis on medicinal plants. Fungal Diversity 131: 99–299. https://doi.org/10.1007/s13225-024-00549-4
  39. Xu, L., Yang, H., Li, C., Liu, S., Zhao, H., Liao, X. & Zhao, L. (2023) Composition analysis of free and bound phenolics in chestnut rose (Rosa roxburghii Tratt.) fruit by UHPLC-IM-QTOF and UPLC-QQQ. LWT 185: 115125. https://doi.org/10.1016/j.lwt.2023.115125
  40. Vaidya, G., Lohman, D.J. & Meier, R. (2011) SequenceMatrix: concatenation software for the fast assembly of multi-gene datasets with character set and codon information. Cladistics 27: 171–180. https://doi.org/10.1111/j.1096-0031.2010.00329.x
  41. Vidal, A., Parada, R., Mendoza, L. & Cotoras, M. (2020) Endophytic fungi isolated from plants growing in central Andean Precordillera of Chile with antifungal activity against Botrytis cinerea. Journal of Fungi 6 (3): 149. https://doi.org/10.3390/jof6030149
  42. Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
  43. Wang, L., Feng, S., Luo, X. & Zhao, C. (2024) First Report of Rosa roxburghii Rot Disease Caused by Fusarium annulatum in China. Plant Disease 108 (10): 3187.
  44. Wu, N., Du, H.Z., Chethana, K.W.T., Khongphinitbunjong, K., Maharachchikumbura, S.S.N., Hyde, K.D. & Liu, J.K. (2024) Additions to Dictyosporiaceae: Neoxylochrysis typhicola comb. et gen. nov., two new species and four new host records from medicinal plants in Southwestern China. Journal of Fungi 10 (12): 872. https://doi.org/10.3390/jof10120872
  45. Wei, C., Deng, Q., Sun, M. & Xu, J. (2020) Cytospyrone and cytospomarin: Two new polyketides isolated from mangrove endophytic fungus, Cytospora sp. Molecules 25 (18): 4224. https://doi.org/10.3390/molecules25184224
  46. White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR protocols: A guide to the methods and applications. New York, NY, Academic Press, pp. 315–322.
  47. Wijayawardene, N.N., Hyde, K.D., Dai, D.Q., Sánchez-García, M., Goto, B.T., Saxena, R.K., Erdoğdu, M., Selçuk, F., Rajeshkumar, K.C., Aptroot, A., Błaszkowski, J., Boonyuen, N., da Silva, G.A., de Souza, F.A., Dong, W., Ertz, D., Haelewaters, D., Jones, E.B.G., Karunarathna, S.C., Kirk, P.M., Kukwa, M., Kumla, J., Leontyev, D.V., Lumbsch, H.T., Maharachchikumbura, S.S.N., Marguno, F., Martínez-Rodríguez, P., Mešić, A., Monteiro, J.S., Oehl, F., Pawłowska, J., Pem, D., Pfliegler, W.P., Phillips, A.J.L., Pošta, A., He, M.Q., Li, J.X., Raza, M., Sruthi, O.P., Suetrong, S., Suwannarach, N., Tedersoo, L., Thiyagaraja, V., Tibpromma, S., Tkalčec, Z. & Tokarev, Y.S., Wanasinghe, D.N., Wijesundara, D.S.A. & Wimalaseana, S.D.M.K., Madrid, H., Zhang, G.Q., Gao, Y., Sánchez-Castro, I., Tang, L.Z., Stadler, M., Yurkov, A. & Thines, M. (2022) Outline of Fungi and fungus-like taxa – 2021. Mycosphere 13 (1): 53–453. https://doi.org/10.5943/mycosphere/13/1/2
  48. Zhang, D., Gao, F., Jakovlić, I., Zou, H., Zhang, J., Li, W.X. & Wang, G.T. (2020) PhyloSuite: an integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies. Molecular Ecology Resources 20: 348–355. https://doi.org/10.1111/1755-0998.13096
  49. Zhang, H., Wei, T.P., Li, L.Z., Luo, M.Y., Jia, W.Y., Zeng, Y., Jiang, Y.L. & Tao, G.C. (2021) Multigene phylogeny, diversity and antimicrobial potential of endophytic Sordariomycetes from Rosa roxburghii. Frontiers in Microbiology 12: 755919. https://doi.org/10.3389/fmicb.2021.755919
  50. Zhang, H., Yang, M.F., Zhang, Q., Yan, B. & Jiang, Y.L. (2022) Screening for broad-spectrum antimicrobial endophytes from Rosa roxburghii and multi-omic analyses of biosynthetic capacity. Frontiers in Plant Science 13: 1060478. https://doi.org/10.3389/fpls.2022.1060478

How to Cite

Zhang, H. & Jiang, Y.-L. (2025) Cytospora guizhouensis sp. nov., an endophytic fungus from Rosa roxburghii in Guizhou, China. Phytotaxa 722 (3): 207–221. https://doi.org/10.11646/phytotaxa.722.3.1