Skip to main content Skip to main navigation menu Skip to site footer
Type: Article
Published: 2025-10-21
Page range: 24-40
Abstract views: 268
PDF downloaded: 10

Distoseptispora pulchra sp. nov. and D. liupanshuiensis from freshwater habitats in Yunnan Province, China

College of Agriculture and Biological Science, Dali University, Dali 671003, Yunnan, P.R. China
College of Agriculture and Biological Science, Dali University, Dali 671003, Yunnan, P.R. China
College of Agriculture and Biological Science, Dali University, Dali 671003, Yunnan, P.R. China
College of Agriculture and Biological Science, Dali University, Dali 671003, Yunnan, P.R. China; Co-Innovation Center for Cangshan Mountain and Erhai Lake Integrated Protection and Green Development of Yunnan Province, Dali University, Dali 671003, Yunnan, P.R. China
College of Agriculture and Biological Science, Dali University, Dali 671003, Yunnan, P.R. China
1 new species Distoseptisporaceae morphology phylogeny taxonomy Fungi

Abstract

During an investigation into the species diversity of lignicolous freshwater fungi in Yunnan Province, China, four strains of Distoseptispora were collected. Based on morphological characteristics and phylogenetic analysis of combined ITS, LSU, tef1-α, and rpb2 sequence data, Distoseptispora pulchra sp. nov., and one known species, D. liupanshuiensis, were identified. Distoseptispora pulchra is characterized by macronematous, mononematous, unbranched, cylindrical, septate, pale olive-brown conidiophores with monoblastic, terminal, determinate, cylindrical, pale brown to olive-brown, conidiogenous cells that produce and acrogenous, solitary, obclavate, olivaceous to pale brown, distoseptate conidia. In addition, D. liupanshuiensis is reported here for the first time from a freshwater habitat. Detailed descriptions, phylogenetic analysis results, and illustrations of both species are provided, along with a morphological comparison with related taxa.

References

  1. Afshari, N., Gomes de Farias, A.R., Bhunjun, C.S., Phukhamsakda, C., Hyde, K.D. & Lumyong, S. (2023) Distoseptispora dipterocarpi sp. nov. (Distoseptisporaceae), a lignicolous fungus on decaying wood of Dipterocarpus in Thailand. Journal of Fungal Biology 13 (1): 68–78. https://doi.org/10.5943/cream/13/1/5
  2. Ahmadpour, A., Ghosta, Y., Heidarian, Z., Alavi, Z. & Alavi, F. (2023) Morphological and molecular characterization of Distoseptispora bambusae from Iran. Mycologia Iranica 10 (2): 1–10. https://doi.org/10.1080/00275514.2025.2450754
  3. Bao, D.F., Luo, Z.L., Liu, J.K., Bhat, D.J., Sarunya, N., Li, W.L., Su, H.Y. & Hyde, K.D. (2018) Lignicolous freshwater fungi in China III: New species and record of Kirschsteiniothelia from northwestern Yunnan Province. Mycosphere 9: 755–768. https://doi.org/10.5943/mycosphere/9/4/4
  4. Barros, J. & Seena, S. (2022) Fungi in freshwaters: prioritising aquatic hyphomycetes in conservation goals. Water 14 (4): e605. https://doi.org/10.3390/w14040605
  5. Cai, L., Jeewon, R. & Hyde, K.D. (2006) Phylogenetic investigations of Sordariaceae based on multiple gene sequences and morphology. Mycological Research 110 (2): 137–150. https://doi.org/10.1016/j.mycres.2005.09.014
  6. Capella-Gutiérrez, S., Silla-Martínez, J.M. & Gabaldón, T. (2009) trimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses. Bioinformatics 25 (15): 1972–1973. https://doi.org/10.1093/bioinformatics/btp348
  7. Crous, P.W., Wingffeld, M.J., Lombard, L., Roets, F., Swart, W.J., Alvarado, P., Carnegie, A.J., Moreno, G., Luangsaard, J., Thangavel, R., Alexandrova, A.V., Baseia, I.G., Bellanger, J.M., Bessette, A.J., Bessette, A.R., Peña-Lastra, S.D.L., García, D., Gené, J., Pham, T.H.G., Heykoop, M., Malysheva, E., Malysheva, V., Martín, M.P., Morozova, O.V., Noisripoom, W., Overton, B.E., Rea, A.E., Sewall, B.J., Smith, M.E., Smyth, C.W., Tasanathai, K., Visagie, C.M., Adamčík, S., Alves, A., Andrade, J.P., Aninat, M.J., Araújo, R.V.B., Bordallo, J.J., Bouffeur, T., Baroncelli, R., Barreto, R.W., Bolin, J., Cabero, J., Caboň, M., Cafà, G., Caffot, M.L.H., Cai, L., Carlavilla, J.R., Chávez, R., Castro, R.R.L.D., Delgat, L., Deschuyteneer, D., Dios, M.M., Domínguez, LS., Evans, H.C., Eyssartier, G., Ferreira, B.W., Figueiredo, C.N., Liu, F., Fournier, J., Galli-Terasawa, L.V., Gil-Durán, C., Glienke, C., Gonçalves, M.F.M., Gryta, H., Guarro, J., Himaman, W., Hywel-Jones, N., Iturrieta-González, I., Ivanushkina, N.E., Jargeat, P., Khalid, A.N., Khan, J., Kiran, M., Kiss, L., Kochkina, J.A., Kolařík, M., Kubátová, A., Lodge, D.J., Loizides, M., Luque, D., Manjón, J.L., Marbach, P.A.S., Massola, N.S., Mata, M., Miller, A.N., Mongkolsamrit, S., Moreau, P.A., Morte, A., Mujic, A., Navarro-Ródenas, A., Németh, M.Z., Nóbrega, T.F., Nováková, A., Olariaga, I., Ozerskaya, S.M., Palma, M.A., Petters-Vandresen, D.A.L., Piontelli, E., Popov, E.S., Rodríguez, A., Requejo, Ó., Rodrigues, A.C.M., Rong, I.H., Roux, J., Seifert, K.A., Silva, B.D.B., Sklenář, F., Smith, J.A., Sousa, J.O., Souza, H.G., Souza, J.T.D., Švec, K., Tanchaud, P., Tanney, J.B., Terasawa, F., Thanakitpipattana, D., Torres-Garcia, D., Vaca, I., Vagheff, N., Iperen, A.L.V., Vasilenko, O.V., Verkbeen, A., Yilmaz, N., Zamora, J.C., Zapata, M., Jurjević, Ž. & Groenewald, J.Z. (2019) Fungal Planet description sheets: 951–1041. Persoonia 43 (1): 223–425. https://doi.org/10.3767/persoonia.2019.43.06
  8. Chethana, K.W.T., Manawasinghe, I.S., Hurdeal, V.G., Bhunjun, C.S., Appadoo, M.A. & Gentekaki, E. (2021) What are fungal species and how to delineate them? Fungal Diversity 109: 1–25. https://doi.org/10.1007/s13225-021-00483-9
  9. Calabon, M.S., Hyde, K.D., Jones, E.B.G., Bao, D.F., Bhunjun, C.S., Phukhamsakda, C., Shen, H.W., Gentekaki, E., Al, Sharie, A.H., Barros, J., Chandrasiri, K.S.U., Hu, D.M., Hurdeal, V.G., Rossi, W., Valle, L.G., Zhang, H., Figueroa, M., Raja, H.A., Seena, S., Song, H.Y., Dong, W., El-Elimat, T., Leonardi, M., Li, Y., Li, Y.J., Luo, Z.L., Ritter, C.D. & Strongman, D.B. (2023) Freshwater fungal biology. Mycosphere 14: 195–413. https://doi.org/10.5943/mycosphere/14/1/4
  10. Chen, X.M., Tang, X., Tang, X., Ma, J., Liu, N.G., Saowaluck, T., Tibpromma, S., Karunarathna, S.C., Xiao, Y.P. & Lu, Y.Z. (2024) Identification of two new species and a new host record of Distoseptispora (Distoseptisporaceae, Distoseptisporales, Sordariomycetes) from terrestrial and freshwater habitats in Southern China. MycoKeys 102: 83–105. https://doi.org/10.3897/mycokeys.102.115452
  11. Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: More models, new heuristics and parallel computing. Nature Methods 9 (8): 772. https://doi.org/10.1038/nmeth.2109
  12. Duarte, S., Fernandes, I., Nogueira, M.J., Cássio, F. & Pascoal, C. (2013) Temperature alters interspecific relationrelationships among aquatic fungi. Fungal Ecology 6 (3): 187–191. https://doi.org/10.1016/j.funeco.2013.02.001
  13. Dong, W., Wang, B., Hyde, K.D., McKenzie, E.H., Raja, H.A., Tanaka, K., Abdel-Wahab, M.A., Abdel-Aziz, F.A., Doilom, M., Phookamsak, R., Hongsanan, S., Wanasinghe, D.N., Yu, X.D., Wang, G.N., Yang, H., Yang, J., Thambugala, K.M., Tian, Q., Luo, Z.L., Yang, J.B., Miller, A.N., Fournier, J., Boonmee, S., Hu, D.M., Nalumpang, S. & Zhang, H. (2020) Freshwater Dothideomycetes. Fungal Diversity 105: 319–575. https://doi.org/10.1007/s13225-020-00463-5
  14. Dissanayake, L.S., Samarakoon, M.C., Maharachchikumbura, S.S.N., Hyde, K.D., Tang, X., Li, Q.R., Mortimer, P.E., Faraj, T., Xu, J.C., Kang, J.C. & Wanasinghe, D.N. (2024) Exploring the taxonomy and phylogeny of Sordariomycetes taxa emphasizing Xylariomycetidae in Southwestern China. Mycosphere 15 (1): 1675–1793. https://doi.org/10.5943/mycosphere/15/1/15
  15. Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.
  16. Hyde, K.D., Fryar, S., Tian, Q., Bahkali, A.H. & Xu, J.C. (2016a) Lignicolous freshwater fungi along a northsouth latitudinal gradient in the Asian/Australian region; can we predict the impact of global warming on biodiversity and function? Fungal Ecology 19: 190–200. https://doi.org/10.1016/j.funeco.2015.07.002
  17. Hyde, K.D., Hongsanan, S., Jeewon, R., Bhat, D.J., McKenzie, E.H.C., Jones, E.B.G., Phookamsak, R., Ariyawansa, H.A., Boonmee, S., Zhao, Q., Abdel-Aziz, F.A., Abdel-Wahab, M.A., Banmai, S., Chomnunti, P., Cui, B.K., Daranagama, D.A., Das, K., Dayarathne, M.C., Silva, N.I.D., Goes-Neto, A.A., Huang, S.K., Jayasiri, S.C., Jayawardena, R.S., Konta, S., Lee, H.B., Li, W.J., Lin, C.G., Liu, J.K., Lu, Y.Z., Luo, Z.L., Manawasinghe, I.S., Manimohan, P., Mapook, A., Niskanen, T., Norphan-phoun, C., Papizadeh, M., Perera, R.H., Phukhamsakda, C., Richter, C., Santiago, A.L.C.M.D.A., Drechsler-Santos, E.R., Senanayake, I.C., Tanaka, K., Tennakoon, T.M.D.S., Thambugala, K.M., Tian, Q., Tibpromma, S., Thongbai, B., Vizzini, A., Wanasinghe, D.N., Wijayawardene, N.N., Wu, H.X., Yang, J., Zeng, X.Y., Zhang, H., Zhang, J.F., Bulgakov, T.S., Camporesi, E., Bahkali, A.H., Amoozegar, M.A., Araujo-Neta, L.S., Ammirati, J.F., Baghela, A., Bhatt, R.P., Bojantchev, D., Buyck, B., Silva, G.A.D., Lima, C.L.F.D., Oliveira, R.J.V.D., Souza, C.A.F.D., Dai, Y.C., Dima, B., Duong, T.T., Er-cole, E., Mafalda-Freire, F., Ghosh, A., Hashimoto, A., Kamolhan, S., Kang, J.C., Karunarathna, S.C., Kirk, P.M., Kytovuori, I., Lantieri, A., Liimatainen, K., Liu, Z.Y., Liu, X.Y., Lucking, R., Medardi, G., Mortimer, P.E., Nguyen, T.T.T., Promputtha, I., Raj, K.N.A., Reck, M.A., Lumyong, S., Shahza-deh-Fazeli, S.A., Stadler, M., Soudi, M.R., Su, H.Y., Takahashi, T., Tangthirasunun, N., Uniyal, P., Wang, Y., Wen, T.C., Xu, J.C., Zhang, Z.K., Zhao, Y.C., Zhou, J.L. & Zhu, L. (2016b) Fungal diversity notes 367–490: taxonomic and phylogenetic contributions to fungal taxa. Fungal Diversity 80: 1–270. https://doi.org/10.1007/s13225-016-0373-x
  18. Hyde, K.D., Tennakoon, D.S., Jeewon, R., Bhat, D.J., Maharachchikumbura, S.S.N., Rossi, W., Leonardi, M., Lee, H.B., Mun, H.Y., Houbraken, J., Nguyen, T.T.T., Jeon, S.J., Frisvad, J.C., Wanasinghe, D.N., Lücking, R., Aptroot, A., Cáceres, M.E.S., Karunarathna, S.C., Hongsanan, S., Phookamsak, R., Silva, N.I., Thambugala, K.M., Jayawardena, R.S., Senanayake, I.C., Boonmee, S., Chen, J., Luo, Z.L., Phukhamsakda, C., Pereira, O.L., Abreu, V.P., Rosado, A.W.C., Bart, B., Randrianjohany, E., Hofstetter, V., Gibertoni, T.B., Soares, A.W.S., PlautzJr, H.L., Sotão, H.M.P., Xavier, W.K.S., Bezerra, J.D.P., Oliveira, T.G.L., Souza-Motta, C.M., Magalhães, O.M.C., Bundhun, D., Harishchandra, D., Manawasinghe, I.S., Dong, W., Zhang, S.N., Bao, D.F., Samarakoon, M.C., Pem, D., Karunarathna, A., Lin, C.G., Yang, J., Perera, R.H., Kumar, V., Huang, S.K., Dayarathne, M.C., Ekanayaka, A.H., Jayasiri, S.C., Xiao, Y.P., Konta, S., Niskanen, T., Liimatainen, K., Dai, Y.C., Ji, X.H., Tian, X.M., Mešić, A., Singh, S.K., Phutthacharoen, K., Cai, L., Sorvongxay, T., Thiyagaraja, V., Norphanphoun, C., Chaiwan, N., Lu, Y.Z., Jiang, H.B., Zhang, J.F., Abeywickrama, P.D., Aluthmuhandiram, J.V.S., Brahmanage, R.S., Zeng, M., Chethana, T., Wei, D.P., Réblová, M., Fournier, J., Nekvindová, J., Barbosa, R.N., Santos, J.E.F., Oliveira, N.T., Li, G.J., Ertz, D., Shang, Q.J., Phillips, A.J.L., Kuo, C.H., Camporesi, E., Bulgakov, T.S., Lumyong, S., Jones, E.B.G., Chomnunti, P., Gentekaki, E., Bungartz, F., Zeng, X.Y., Fryar, S., Tkalčec, Z., Liang, J.M., Li, G.S., Wen, T.C., Singh, P.N., Gafforov, Y., Promputtha, I., Yasanthika, E., Goonasekara, I.D., Zhao, R.L., Zhao, Q., Kirk, P.M., Liu, J.K., Yan, J.Y., Mortimer, P.E. & Xu, J.C. (2019) Fungal diversity notes 1036–1150: Taxonomic and phylogenetic contributions on genera and species of fungal taxa. Fungal Diversity 96: 1–242. https://doi.org/10.1007/s13225-019-00429-2
  19. Hyde, K.D., Bao, D.F., Hongsanan, S., Chethana, K.W.T., Yang, J. & Suwannarach, N. (2021) Evolution of freshwater Diaporthomycetidae (Sordariomycetes) provides evidence for five new orders and six new families. Fungal Diversity 107: 71–105. https://doi.org/10.1007/s13225-021-00469-7
  20. Hyde, K.D., Norphanphoun, C., Ma, J., Yang, H.D., Zhang, J.Y., Du, T.Y., Gao, Y, Gomes de Farias, A.R., Gui, H., He, S.C., He, Y.K., Li, C.J.Y., Liu, X.F., Lu, L., Su, H.L., Tang, X., Tian, X.G., Wang, S.Y., Wei, D.P., Xu, R.F., Xu, R.J., Yang, Q., Yang, Y.Y., Zhang, F., Zhang, Q., Bahkali, A.H., Boonmee, S., Chethana, K.W.T., Jayawardena, R.S., Lu, Y.Z., Karunarathna, S.C., Tibpromma, S. & Wang, Y. & Zhao, Q. (2023) Mycosphere notes 387–412–novel species of fungal taxa from around the world. Mycosphere 14 (1): 663–744. https://doi.org/10.5943/mycosphere/14/1/8
  21. Hu, Y.F., Liu, J.W., Luo, X.X., Xu, Z.H., Xia, J.W., Zhang, X.G., Castañeda-Ruíz, R.F. & Ma, J. (2023) Multilocus phylogenetic analyses reveal eight novel species of Distoseptispora from southern China. Microbiology Spectrum 11 (6): e02468–23. https://doi.org/10.1128/spectrum.02468-23
  22. Koske, R.E. & Duncan, I.W. (1974) Temperature effects on growth, sporulation, and germination of some “aquatic” hyphomycetes. Canadian Journal of Botany 52: 1387–1391. https://doi.org/10.1139/b74-180
  23. Kuraku, S., Zmasek, C.M., Nishimura, O. & Katoh, K. (2013) ALeaves facilitates on-demand exploration of metazoan gene family trees on MAFFT sequence alignment server with enhanced interactivity. Nucleic Acids Research 41: W22–W28. https://doi.org/10.1093/nar/gkt389
  24. Katoh, K., Rozewicki, J. & Yamada, K.D. (2019) MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Brief Bioinfor 20 (4): 1160–1166. https://doi.org/10.1093/bib/bbx108
  25. Konta, S., Tibpromma, S., Karunarathna, S.C., Samarakoon, M.C., Steven, L.S., Mapook, A., Boonmee, S., Senwanna, C., Balasuriya, A. & Eungwanichayapant, P.D. & Hyde, K.D. (2023) Morphology and multigene phylogeny reveal ten novel taxa in Ascomycota from terrestrial palm substrates (Arecaceae) in Thailand. Mycosphere 14 (1): 107–152. https://doi.org/10.5943/mycosphere/14/1/2
  26. Karimi, O., Thilini, C.K.W., Farias, A.R.G., Asghari, f., Kaewchai, S., Hyde, K.D. & Li, Q.R. (2024) Morphology and multigenephylogeny reveal three new species of Distoseptispora (Distoseptisporales, Distoseptisporaceae) on palms (Arecaceae) from peatswamp areas in southern Thailand. MycoKeys 102: 55–81. https://doi.org/10.3897/mycokeys.102.112815
  27. Liu, Y.J., Whelen, S. & Hall, B.D. (1999) Phylogenetic relationships among ascomycetes: evidence from an RNA polymerase II subunit. Molecular Biology and Evolution 16 (12): 1799–1808. https://doi.org/10.1093/oxfordjournals.molbev.a026092
  28. Liu, J.K., Phookamsak, R., Doilom, M., Wikee, S., Li, Y.M., Ariyawansha, H., Boonmee, S., Chomnunti, P., Dai, D.Q., Bhat, J.D., Romero, A.I., Zhang, W.Y., Monkai, J., Jones, E.B.G., Chukeatirote, E., KoKo, T.W., Zhao, Y.C., Wang, Y. & Hyed, K.D. (2012) Towards a natural classification of Botryosphaeriales. Fungal Diversity 57: 149–210. https://doi.org/10.1007/s13225-012-0207-4
  29. Larsson, A. (2014) AliView: a fast and lightweight alignment viewer and editor for large datasets. Bioinformatics 30 (22): 3276–3278. https://doi.org/10.1093/bioinformatics/btu531
  30. Luo, Z.L., Hyde, K.D., Liu, J.K., Bhat, D.J., Bao, D.F., Li, W.L., Su, X.J., Yang, X.Y. & Su, H.Y. (2018) Lignicolous freshwater fungi from China II: novel Distoseptispora (Distoseptisporaceae) species from northwestern Yunnan Province and a suggested unified method for studying lignicolous freshwater fungi. Mycosphere 9 (3): 444–461. https://doi.org/10.5943/mycosphere/9/3/2
  31. Luo, Z.L., Hyde, K.D., Liu, J.K., Maharachchikumbura, S.S.N., Jeewon, R., Bao, D.F., Bhat, D.J., Lin, C.G., Li, W.L., Yang, J., Liu, N.G., Lu, Y.Z., Jayawardena, R.S., Li, J.F. & Su, H.Y. (2019) Freshwater Sordariomycetes. Fungal Diversity 99: 451–660. https://doi.org/10.1007/s13225-019-00438-1
  32. Liu, J.W., Hu, Y.F., Luo, X.X., Xu, Z.H., Castañeda-Ruíz, R.F., Xia, J.W., Zhang, X.G., Zhang, L.H., Cui, R.Q. & Ma, J. (2023) Morphological and phylogenetic analyses reveal three new species of Distoseptispora (Distoseptisporaceae, Distoseptisporales) from Yunnan, China. Journal of Fungi 9 (4): e470. https://doi.org/10.3390/jof9040470
  33. Liao, M.G., Luo, X.X., Hu, Y.F., Castañeda-Ruíz, R.F., Xu, Z.H. & Ma, J. (2025) Morphological and phylogenetic analyses reveal four novel species of Distoseptispora (Distoseptisporaceae, Distoseptisporales) from southern China. MycoKeys 113: 31–55. https://doi.org/10.3897/mycokeys.113.137082
  34. McKenzie, E.H.C. (1995) Dematiaceous hyphomycetes on Pandanaceae 5.Sporidesmium sensu lato. Mycotaxon 56: 9–29.
  35. Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: 2010 gateway computing environments workshop (GCE). pp. 1–8. https://doi.org/10.1109/GCE.2010.5676129
  36. Monkai, J., Boonmee, S., Ren, G.C., Wei, D.P., Phookamsak, R. & Mortimer, P.E. (2020) Distoseptispora hydei sp. nov. (Distoseptisporaceae), a novel lignicolous fungus on decaying bamboo in Thailand. Phytotaxa 459 (2): 93–107. https://doi.org/10.11646/phytotaxa.459.2.1
  37. Ma, J., Zhang, J.Y., Xiao, X.J., Xiao, Y.P., Tang, X., Boonmee, S., Kang, J.C. & Lu, Y.Z. (2022) Multigene phylogenetic analyses revealed five new species and two new records of Distoseptisporales from China. Journal of Fungi 8 (11): e1202. https://doi.org/10.3390/jof8111202
  38. Nylander, J. (2004) MrModeltest v2. Program distributed by the author: Evolutionary Biology Centre. Uppsala University, Sweden. [http://www.ebc.uu.se/systzoo/staff/nylander.html]
  39. Rambaut, A. (2014) FigTree 1.4.4. Available from: http://tree.bio.ed.ac.uk/software/figtree (accessed 9 November 2024)
  40. Phookamsak, R., Hyde, K.D., Jeewon, R., Bhat, D.J., Jones, E.B.G., Maharachchikumbura, S.S.N., Raspé, O., Karunarathna, S.C., Wanasinghe, D.N., Hongsanan, S., Doilom, M., Tennakoon, D.S., Machado, A.R., Firmino, A.L., Ghosh, A., Karunarathna, A., Mešić, A., Dutta, A.K., Thongbai, B., Devadatha, B., Norphanphoun, C., Senwanna, C., Wei, D.P., Pem, D., Ackah, F.K., Wang, G.N., Jiang, H.B., Madrid, H., Lee, H.B., Goonasekara, I.D., Manawasinghe, I.S., Kušan, I., Cano, J., Gené, J., Li, J.F., Das, K., Acharya, K., Raj, K.N.A., Latha, K.P.D., Chethana, K.W.T., He, M.Q., Dueñas, M., Jadan, M., Martín, M.P., Samarakoon, M.C., Dayarathne, M.C., Raza, M., Park, M.S., Telleria, M.T., Chaiwan, N., Matočec, N., Silva, N.I., Pereira, O.L., Singh, P.N., Manimohan, P., Uniya, P., Shang, Q.J., Bhatt, R.P., Perera, R.H., Alvarenga, R.L.M., Nogal-Prata, S., Singh, S.K., Vadthanarat, S., Oh, S.Y., Huang, S.K., Rana, S., Konta, S., Paloi, S., Jayasiri, S.C., Jeon, S.J., Mehmood, T., Gibertoni, T.B., Nguyen, T.T.T., Singh, U., Thiyagaraja, V., Sarma, V.V., Dong, W., Yu, X.D., Lu, Y.Z., Lim, Y.W., Chen, Y., Tkalčec, Z., Zhang, Z.F., Luo, Z.L., Daranagama, D.A., Thambugala, K.M., Tibpromma, S. & Camporesi, E., Bulgakov, T.S., Dissanayake, A.J., Senanayake, I.C., Dai, D.Q., Tang, L.Z., Khan, S., Zhang, H., Promputtha, I., Cai, L., Chomnunti, P., Zhao, R.L., Lumyong, S., Boonmee, S., Wen, T.C., Mortimer, P.E. & Xu, J.C. (2019) Fungal diversity notes 929–1035: Taxonomic and phylogenetic contributions on genera and species of fungi. Fungal Diversity 95: 1–273. https://doi.org/10.1007/s13225-019-00421-w
  41. Phukhamsakda, C., McKenzie, E.H.C., Phillips, A.J.L., Jones, E.B.G., Bhat, D.J., Marc, S., Bhunjun, C.S., Wanasinghe, D.N., Thongbai, B., Camporesi, E., Ertz, D., Jayawardena, R.S., Perera, R.H., Ekanayake, A.H., Tibpromma, S., Doilom, M., Xu, J.C. & Hyde, K.D. (2020) Microfungi associated with Clematis (Ranunculaceae) with an integrated approach to delimiting species boundaries. Fungal Diversity 102 (1): 1–203. https://doi.org/10.1007/s13225-020-00448-4
  42. Phukhamsakda, C., Nilsson, R.H., Bhunjun, C.S., de Farias, A.R., Sun, Y.R., Wijesinghe, S.N., Raza, M., Bao, D.F., Lu, L., Tibpromma, S., Dong, W., Tennakoon, D.S., Tian, X.G., Xiong, Y.R., Karunarathna, S.C., Cai, L., Luo, Z., Wang, Y., Manawasinghe, I.S., Camporesi, E., Kirk, P.M., Promputtha, I., Kuo, C.H., Su, H.Y., Doilom, M., Li, Y., Fu, Y.P. & Hyde, K.D. (2022) The numbers of fungi: contributions from traditional taxonomic studies and challenges of metabarcoding. Fungal Diversity 114: 327–386. https://doi.org/10.1007/s13225-022-00502-3
  43. Qian, L.S., Chen, J.H., Deng, T. & Sun, H. (2020) Plant diversity in Yunnan: Current status and future directions. Plant Diversity 42 (4): 281–291. https://doi.org/10.1016/j.pld.2020.07.006
  44. Rannala, B. & Yang, Z. (1996) Probability distribution of molecular evolutionary trees: a new method of phylogenetic inference. Journal Molecular Evolution 43: 304–311. https://doi.org/10.1007/BF02338839
  45. Ronquist, F., Teslenko, M., Van, Der, Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L. & Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Systems Biology 61 (3): 539–542. https://doi.org/10.1093/sysbio/sys029
  46. Rathnayaka, A.R., Tennakoon, D.S., Jones, G.E., Wanasinghe, D.N., Bhat, D.J., Priyashantha, A.H., Stephenson, S.L., Tibpromma, S. & Karunarathna, S.C. (2025) Significance of precise documentation of hosts and geospatial data of fungal collections, with an emphasis on plant-associated fungi. New Zealand Journal of Botany 63 (2–3): 462–489. https://doi.org/10.1080/0028825X.2024.2381734
  47. Shoemaker, R.A. & White, G.P. (1985) Lasiosphaeria caesariata with Sporidesmium hormiscioides and L. triseptata with S. adscendens. Sydowia 38: 278–283. https://doi.org/10.1093/bioinformatics/btl446
  48. Shenoy, B.D., Jeewon, R., Wu, W.P.P., Bhat, D.J. & Hyde, K.D. (2006) Ribosomal and RPB2 DNA sequence analyses suggest that Sporidesmium and morphologically similar genera are polyphyletic. Mycological Research 110 (8): 916–928. https://doi.org/10.1016/j.mycres.2006.06.004
  49. Stamatakis, A. (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30: 1312–1313. https://doi.org/10.1093/bioinformatics/btu033
  50. Su, H.Y., Hyde, K.D., Maharachchikumbura, S.S.N., Ariyawansa, H.A., Luo, Z.L., Promputtha, I., Tian, Q., Lin, C., Shang, Q. & Zhao, Y. (2016) The families Distoseptisporaceae fam. nov., Kirschsteiniotheliaceae, Sporormiaceae and Torulaceae, with new species from freshwater in Yunnan Province, China. Fungal Diversity 80 (1): 375–409. https://doi.org/10.1007/s13225-016-0362-0
  51. Senanayake, I.C., Rathnayaka, A.R., Marasinghe, D.S., Calabon, M.S., Gentekaki, E., Lee, H.B., Hurdeal, V.G., Pem, D., Dissanayake, L.S. & Wijesinghe, S.N., Bundhun, D., Nguyen, T.T., Goonasekara, I.D., Abeywickrama, P.D., Bhunjun, C.S., Jayawardena, R.S., Wanasinghe, D.N., Jeewon, R., Bhat, D.J. & Xiang, M.M. (2020) Morphological approaches in studying fungi: collection, examination, isolation, sporulation and preservation. Mycosphere 11: 2678–2754. https://doi.org/10.5943/mycosphere/11/1/20
  52. Sun, Y.R., Goonasekara, I.D., Thambugala, K.M., Jayawardena, R.S., Wang, Y. & Hyde, K.D. (2020) Distoseptispora bambusae sp. nov. (Distoseptisporaceae) on bamboo from China and Thailand. Biodiversity Data Journal 8: e53678. https://doi.org/10.3897/BDJ.8.e53678
  53. Shenoy, H.W., Bao, D.F., Hyde, K.D., Su, H.Y., Bhat, D.J. & Luo, Z.L. (2021) Two novel species and two new records of Distoseptispora from freshwater habitats in China and Thailan. MycoKeys 84: 79–101. https://doi.org/10.3897/mycokeys.84.71905
  54. Shen, H.W., Bao, D.F., Bhat, D.J., Su, H.Y. & Luo, Z.L. (2022) Lignicolous freshwater fungi in Yunnan Province, China: an overview. Mycology 13: 119–132. https://doi.org/100.1080/21501203.2022.2058638
  55. Shen, H.W., Bao, D.F., Saranyaphat, B., Su, X.J., Tian, X.G., Hyde, K.D. & Luo, Z.L. (2023) Lignicolous freshwater fungi from plateau lakes in China (I): morphological and phylogenetic analyses reveal eight species of Lentitheciaceae, including new genus, new species and new records. Journal of Fungi 9 (10): e962. https://doi.org/10.3390/jof9100962
  56. Shen, H.W., Bao, D.F., Boonmee, S., Lu, Y.Z., Su, X.J., Li, Y.X. & Luo, Z.L. (2024) Diversity of Distoseptispora (Distoseptisporaceae) taxa on submerged decaying wood from the Red River in Yunnan, China. MycoKeys 102: 1–28. https://doi.org/10.3897/mycokeys.102.116096
  57. Tibpromma, S., Hyde, K.D., McKenzie, E.H.C., Bhat, D.J., Phillips, A.J.L., Wanasinghe, D.N., Sama-rakoon, M.C., Jayawardena, R.S., Dissanayake, A.J., Tennakoon, D.S., Doilom, M., Phookam-sak, R., Tang, A.M.C., Xu, J., Mortimer, P.E., Promputtha, I., Maharachchikumbura, S.S.N., Khan, S. & Karunarathna, S.C. (2018) Fungal diversity notes 840–928: Micro-fungi associated with Pandanaceae. Fungal Diversity 93: 1–160. https://doi.org/10.1007/s13225-018-0408-6
  58. Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
  59. Vaidya, G., Lohman, D.J. & Meier, R. (2011) SequenceMatrix: Concatenation software for the fast assembly of multi‐gene datasets with character set and codon information. Cladistics 27 (2): 171–180. https://doi.org/10.1111/j.1096-0031.2010.00329.x
  60. White, T.J., Bruns, T., Lee, S. & Taylor, J.W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols: A Guide to Methods and Applications 18: 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
  61. Wang, G.N., Yu, X.D., Dong, W., Bhat, D.J., Boonmee, S., Zhang, D. & Zhang, H. (2019) Freshwater hyphomycetes in Eurotiomycetes: a new species of Minimelanolocus and a new collection of Thysanorea papuana (Herpotrichiellaceae). Mycological Progress 18: 511–522. https://doi.org/10.1007/s11557-019-01473-7
  62. Wang, L.S., Zong, S.K. & Zhu, M.L. (2022) Chemical and bioactive diversity of secondary metabolites from freshwater fungi. Chinese Journal of Biochemistry and Molecular Biology 39 (3): 385–399.
  63. [https://link.cnki.net/doi/10.13865/j.cnki.cjbmb.2022.05.1179]
  64. Wang, F., Wang, K., Cai, L., Zhao, M.J., Paul M Kirk, Fan, G.M., Sun, Q.L., Li, B., Wang, S., Yu, Z.F., Han, D., Ma, J.C., Wu, L.H. & Yao, Y.J. (2022) Fungal names: a comprehensive nomenclatural repository and knowledge base for fungal taxonomy. Nucleic Acids Research 51 (D1): D708–D716. https://doi.org/10.1093/nar/gkac926
  65. Yang, J., Maharachchikumbura, S.S.N., Liu, J.K., Hyde, K.D., Jones, E.B.G., Al-Sadi, A.M. & Liu, Z.Y. (2018) Pseudostanjehughesia aquitropica gen. et sp. nov. and Sporidesmium sensu lato species from freshwater habitats. Mycological Progress 17 (5): 591–616. https://doi.org/10.1007/s11557-017-1339-4
  66. Yang, J., Liu, L.L., Jones, E.B., Li, W.L., Hyde, K.D. & Liu, Z.Y. (2021) Morphological variety in Distoseptispora and introduction of six novel species. Journal of Fungi 7 (11): е945. https://doi.org/10.3390/jof7110945
  67. Yu, X.D., Zhang, S.N., Liang, X.D., Zhu, J.T., Hyde, K.D. & Liu, J.K. (2024) Bambusicolous Fungi from Southwestern China. Mycosphere 15 (1): 5038–5145. https://doi.org/10.5943/mycosphere/15/1/24
  68. Zare-Maivan, H. & Shearer, C.A. (1988) Extracellular enzyme production and cell wall degradation by freshwater lignicolous fungi. Mycologia 80 (3): 365–375. https://doi.org/10.1080/00275514.1988.12025551
  69. Zhou, S.Y., Zhou, X., Zhang, Z.F., Liang, J.M. & Cai, L. (2020) Attempt to isolate hitherto uncultured fungi. Mycosystema 39 (4): 766–776. https://doi.org/10.13346/j.mycosystema.190202
  70. Zhai, Z.J., Yan, J.Q., Li, W.W., Gao, Y., Hu, H.J., Zhou, J.P., Song, H.Y. & Hu, D.M. (2022) Three novel species of Distoseptispora (Distoseptisporaceae) isolated from bamboo in Jiangxi Province, China. MycoKeys 88: 35–54. https://doi.org/10.3897/mycokeys.88.79346
  71. Zhang, H., Zhu, R., Qing, Y., Yang, H., Li, C., Wang, G., Zhang, D. & Ning, P. (2022) Polyphasic identification of Distoseptispora with six new species from fresh water. Journal of Fungi 8 (10): e1063. https://doi.org/10.3390/jof8101063
  72. Zheng, J.X., Yang, J.R., Wan, J.F., Wang, Y., Hua, C.L., Yu, C.Y., Cai, W.J., Zhen, J.N. & Wu, F.Q. (2022) Second investigation of key protected wild plant resources in Yunnan Province. Forest Inventory and Plaing 47 (2): 70–76. https://doi.org/10.13428/j.cnki.fjlk.2023.01016
  73. Zhang, L., Shen, H.W., Bao, D.F., Li, L.L., Su, X.J. & Luo, Z.L. (2023) Wongia suae sp. nov. a lignicolous freshwater fungus from Yuanjiang (Red River) Basin, China. Phytotaxa 616 (3): 258–268. https://doi.org/10.11646/phytotaxa.616.3.5

How to Cite

Ma, Q., Wang, Y., Shen, H.-W., Luo, Z.-L. & Su, X.-J. (2025) Distoseptispora pulchra sp. nov. and D. liupanshuiensis from freshwater habitats in Yunnan Province, China. Phytotaxa 725 (1): 24–40. https://doi.org/10.11646/phytotaxa.725.1.3