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Type: Article
Published: 2025-03-06
Page range: 113-124
Abstract views: 115
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A novel species of Blackwellomyces in Guizhou, China

College of Resources and Environment, Zunyi Normal University, Zunyi, Guizhou 563000, China. Engineering Research Center of Southwest Bio-Pharmaceutical Resources, Ministry of Education, Guizhou University, Guiyang 550025, China.
Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand. Innovative Institute for Plant Health, College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, Guangdong, China.
College of Resources and Environment, Zunyi Normal University, Zunyi, Guizhou 563000, China
Guizhou Guiwang Biotechnology Co., Ltd, Daozhen 563599, China
Guizhou Guiwang Biotechnology Co., Ltd, Daozhen 563599, China
Sanjiang Agricultural and Rural Comprehensive Service Center, Daozhen 563500, China
Engineering Research Center of Southwest Bio-Pharmaceutical Resources, Ministry of Education, Guizhou University, Guiyang 550025, China. Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
Engineering Research Center of Southwest Bio-Pharmaceutical Resources, Ministry of Education, Guizhou University, Guiyang 550025, China
1 new species Cordycipitaceae morphology phylogenetic analyses Fungi

Abstract

A new species, Blackwellomyces larvatus, is described and illustrated from Guizhou, representing the first record of Blackwellomyces in this region. This species belongs to the family Cordycipitaceae and was discovered parasitizing a lepidopteran larva. It is characterized by orange stromata and the production of a light purple pigment that diffuses into the medium when cultured on potato dextrose agar. Phylogenetic analyses based on a multi-gene dataset indicate that B. larvatus is phylogenetically close to B. kaihuaensis but forms a distinct lineage, providing support for its recognition as a novel species.

References

  1. Bischoff, J.F., Chaverri, P. & White Jr, J.F. (2005) Clarification of the host substrate of Ascopolyporus and description of Ascopolyporus philodendrus sp. nov. Mycologia 97 (3): 710–717. https://doi.org/10.1080/15572536.2006.11832800
  2. Chaverri, P., Bischoff, J.F., Evans, H.C. & Hodge, K.T. (2005) Regiocrella, a new entomopathogenic genus with a pycnidial anamorph and its phylogenetic placement in the Clavicipitaceae. Mycologia 97 (6): 1225–1237. https://doi.org/10.1080/15572536.2006.11832732
  3. Chuang, W., Lin, Y., Shrestha, B., Luangsa-ard, J., Stadler, M., Tzean, S., Wu, S., Ko, C., Hsieh, S., Wu, M., Shen, T.L. & Ariyawansa, H.A. (2024) Phylogenetic diversity and morphological characterization of cordycipitaceous species in Taiwan. Studies in Mycology 109: 1–56. https://doi.org/10.3114/sim.2024.109.01
  4. Huang, S.K., Hyde, K.D., Maharachchikumbura, S.S.N, McKenzie, E.H.C. & Wen, T.C. (2021) Taxonomic studies of Coronophorales and Niessliaceae (Hypocreomycetidae). Mycosphere 12 (1): 875–992. https://doi.org/10.5943/mycosphere/12/1/9
  5. Hyde, K.D., Tennakoon, D.S., Jeewon, R., Bhat, D.J., Maharachchikumbura, S.S.N., Rossi, W., Leonardi, M., Lee, H.B., Mun, H.Y., Houbraken, J., Nguyen, T.T.T., Jeon, S.J., Frisvad, J.C., Wanasinghe, D.N., Lücking, R., Aptroot, A., Cáceres, M.E.S., Karunarathna, S.C., Hongsanan, S., Phookamsak, R., de Silva, N.I., Thambugala, K.M., Jayawardena, R.S., Senanayake, I.C., Boonmee, S., Chen, J., Luo, Z.-L., Phukhamsakda, C., Pereira, O.L., Abreu, V.P., Rosado, A.W.C., Bart, B., Randrianjohany, E., Hofstetter, V., Gibertoni, T.B., Soares, A.M.S., Plautz Jr., H.L., Sotão, H.M.P., Xavier, W.K.S., Bezerra, J.D.P., de Oliveira, T.G.L., de Souza-Motta, C.M., Magalhães, O.M.C., Bundhun, D., Harishchandra, D., Manawasinghe, I.S., Dong, W., Zhang, S.-N., Bao, D.-F., Samarakoon, M.C., Pem, D., Karunarathna, A., Lin, C.-G., Yang, J., Perera, R.H., Kumar, V., Huang, S.-K., Dayarathne, M.C., Ekanayaka, A.H., Jayasiri, S.C., Xiao, Y., Konta, S., Niskanen, T., Liimatainen, K., Dai, Y.-C., Ji, X.-H., Tian, X.-M., Mešić, A., Singh, S.K., Phutthacharoen, K., Cai, L., Sorvongxay, T., Thiyagaraja, V., Norphanphoun, C., Chaiwan, N., Lu, Y.-Z., Jiang, H.-B., Zhang, J.-F., Abeywickrama, P.D., Aluthmuhandiram, J.V.S., Brahmanage, R.S., Zeng, M., Chethana, T., Wei, D., Réblová, M., Fournier, J., Nekvindová, J., Barbosa, R.N., dos Santos, J.E.F., de Oliveira, N.T., Li, G.-J., Ertz, D., Shang, Q.-J., Phillips, A.J.L., Kuo, C.-H., Camporesi, E., Bulgakov, T.S., Lumyong, S., Jones, E.B.G., Chomnunti, P., Gentekaki, E., Bungartz, F., Zeng, X.-Y., Fryar, S., Tkalčec, Z., Liang, J., Li, G., Wen, T.-C., Singh, P.N., Gafforov, Y., Promputtha, I., Yasanthika, E., Goonasekara, I.D., Zhao, R.-L., Zhao, Q., Kirk, P.M., Liu, J.-K., Yan, J., Mortimer, P.E., Xu, J. & Doilom, M. (2019) Fungal diversity notes 1036–1150: taxonomic and phylogenetic contributions on genera and species of fungal taxa. Fungal Diversity 96: 1–242. https://doi.org/10.1007/s13225-019-00429-2
  6. Hyde, K.D., Noorabadi, M.T., Thiyagaraja, V., He, M.Q., Johnston, P.R., Wijesinghe, S.N., Armand, A., Biketova, A.Y., Chethana, K.W.T., Erdoğdu, M., Ge, Z.W., Groenewald, J.Z., Hongsanan, S., Kušan, I., Leontyev, D.V., Li, D.W., Lin, C.G., Liu, N.G., Maharachchikumbura, S.S.N., Matočec, N., May, T.W., McKenzie, E.H.C., Mešić, A., Perera, R.H., Phukhamsakda, C., Piątek, M., Samarakoon, M.C., Selcuk, F., Senanayake, I.C., Tanney, J.B., Tian, Q., Vizzini, A., Wanasinghe, D.N., Wannasawang, N., Wijayawardene, N.N., Zhao, R.L., Abdel-Wahab, M.A., Abdollahzadeh, J., Abeywickrama, P.D., Abhinav, Absalan, S., Acharya, K., Afshari, N., Afshan, N.S., Afzalinia, S., Ahmadpour, S.A., Akulov, O., Alizadeh, A., Alizadeh, M., Al-Sadi, A.M., Alves, A., Alves, V.C.S., Alves-Silva, G., Antonín, V., Aouali, S., Aptroot, A., Apurillo, C.C.S., Arias, R.M., Asgari, B., Asghari, R., Assis, D.M.A., Assyov, B., Atienza, V., Aumentado, H.D.R., Avasthi, S., Azevedo, E., Bakhshi, M., Bao, D.F., Baral, H.O., Barata, M., Barbosa, K.D., Barbosa, R.N., Barbosa, F.R., Baroncelli, R., Barreto, G.G., Baschien, C., Bennett, R.M., Bera, I., Bezerra, J.D.P., Bhunjun, C.S., Bianchinotti, M.V., Błaszkowski, J., Boekhout, T., Bonito, G.M., Boonmee, S., Boonyuen, N., Bortnikov, F.M., Bregant, C., Bundhun, D., Burgaud, G., Buyck, B., Caeiro, M.F., Cabarroi-Hernández, M., Cai, M.F., Cai, L., Calabon, M.S., Calaça, F.J.S., Callalli, M., Câmara, M.P.S., Cano-Lira, J., Cao, B., Carlavilla, J.R., Carvalho, A., Carvalho, T.G., Castañeda-Ruiz, R.F., Catania, M.D.V., Cazabonne, J., Cedeño-Sanchez, M., Chaiwan, N., Chakraborty, N., Cheewankoon, R., Chen, C., Chen, J., Chen, Q., Chen, Y.P., Chinaglia, S., Coelho-Nascimento, C.C., Coleine, C., Costa-Rezende, D.H., Cortés-Pérez, A., Crouch, J.A., Crous, P.W., Cruz, R.H.S.F., Czachura, P., Damm, U., Darmostuk, V., Daroodi, Z., Das, K., Das, K., Davoodian, N., Davydov, E.A., da Silva, G.A., da Silva, I.R., da Silva, R.M.F., da Silva Santos, A.C., Dai, D.Q., Dai, Y.C., de Groot, M.D., De Kesel, A., De Lange, R., de Medeiros, E.V., de Souza, C.F.A., de Souza, F.A., dela Cruz, T.E.E., Decock, C., Delgado, G., Denchev, C.M., Denchev, T.T., Deng, Y.L., Dentinger, B.T.M., Devadatha, B., Dianese, J.C., Dima, B., Doilom, M., Dissanayake, A.J., Dissanayake, D.M.L.S., Dissanayake, L.S., Diniz, A.G., Dolatabadi, S., Dong, J.H., Dong, W., Dong, Z.Y., Drechsler-Santos, E.R., Druzhinina, I.S., Du, T.Y., Dubey, M.K., Dutta, A.K., Elliott, T.F., Elshahed, M.S., Egidi, E., Eisvand, P., Fan, L., Fan, X., Fan, X.L., Fedosova, A.G., Ferro, L.O., Fiuza, P.O., Flakus, A., W. Fonseca, E.O., Fryar, S.C., Gabaldón, T., Gajanayake, A.J., Gannibal, P.B., Gao, F., García-Sánchez, D., García-Sandoval, R., Garrido-Benavent, I., Garzoli, L., Gasca-Pineda, J., Gautam, A.K., Gené, J., Ghobad-Nejhad, M., Ghosh, A., Giachini, A.J., Gibertoni, T.B., Gentekaki, E., Gmoshinskiy, V., Góes-Neto, A., Gomdola, D., Gorjón, S.P., Goto, B.T., Granados-Montero, M.M., Griffith, G.W., Groenewald, M., Grossart, H.-P., Gu, Z.R., Gueidan, C., Gunarathne, A., Gunaseelan, S., Guo, S.L., Gusmão, L.F.P., Gutierrez, A.C., Guzmán-Dávalos, L., Haelewaters, D., Haituk, H., Halling, R.E., He, S.C., Heredia, G., Hernández-Restrepo, M., Hosoya, T., Hoog, S.D., Horak, E., Hou, C.L., Houbraken, J., Htet, Z.H., Huang, S.K., Huang, W.J., Hurdeal, V.G., Hustad, V.P., Inácio, C.A., Janik, P., Jayalal, R.G.U., Jayasiri, S.C., Jayawardena, R.S., Jeewon, R., Jerônimo, G.H., Jin, J., Jones, E.B.G., Joshi, Y., Jurjević, Ž., Justo, A., Kakishima, M., Kaliyaperumal, M., Kang, G.P., Kang, J.C., Karimi, O., Karunarathna, S.C., Karpov, S.A., Kezo, K., Khalid, A.N., Khan, M.K., Khuna, S., Khyaju, S., Kirchmair, M., Klawonn, I., Kraisitudomsook, N., Kukwa, M., Kularathnage, N.D., Kumar, S., Lachance, M.A., Lado, C., Latha, K.P.D., Lee, H.B., Leonardi, M., Lestari, A.S., Li, C., Li, H., Li, J., Li, Q., Li, Y., Li, Y.C., Li, Y.X., Liao, C.F., Lima, J.L.R., Lima, J.M.S., Lima, N.B., Lin, L., Linaldeddu, B.T., Linn, M.M., Liu, F., Liu, J.K., Liu, J.W., Liu, S., Liu, S.L., Liu, X.F., Liu, X.Y., Longcore, J.E., Luangharn, T., Luangsa-ard, J.J., Lu, L., Lu, Y.Z., Lumbsch, H.T., Luo, L., Luo, M., Luo, Z.L., Ma, J., Madagammana, A.D., Madhushan, A., Madrid, H., Magurno, F., Magyar, D., Mahadevakumar, S., Malosso, E., Malysh, J.M., Mamarabadi, M., Manawasinghe, I.S., Manfrino, R.G., Manimohan, P., Mao, N., Mapook, A., Marchese, P., Marasinghe, D.S., Mardones, M., Marin-Felix, Y., Masigol, H., Mehrabi, M., Mehrabi-Koushki, M., Meiras-Ottoni, A. de, Melo, R.F.R., Mendes-Alvarenga, R.L., Mendieta, S., Meng, Q.F., Menkis, A., Menolli Jr, N., Mikšík, M., Miller, S.L., Moncada, B., Moncalvo, J.M., Monteiro, J.S., Monteiro, M., Mora-Montes, H.M., Moroz, E.L., Moura, J.C., Muhammad, U., Mukhopadhyay, S., Nagy, G.L., Najam ul Sehar, A., Najafiniya, M., Nanayakkara, C.M., Naseer, A., Nascimento, E.C.R., Nascimento, S.S., Neuhauser, S., Neves, M.A., Niazi, A.R., Nie, Y., Nilsson, R.H., Nogueira, P.T.S., Novozhilov, Y.K., Noordeloos, M., Norphanphoun, C., Nuñez, Otaño, N., O’Donnell, R.P., Oehl, F., Oliveira, J.A., Oliveira Junior, I., Oliveira, N.V.L., Oliveira, P.H.F., Orihara, T., Oset, M., Pang, K.L., Papp, V., Pathirana, L.S., Peintner, U., Pem, D., Pereira, O.L., Pérez-Moreno, J., Pérez-Ortega, S., Péter, G., Pires-Zottarelli, C.L.A., Phonemany, M., Phongeun, S., Pošta, A., Prazeres, J.F.S.A., Quan, Y., Quandt, C.A., Queiroz, M.B., Radek, R., Rahnama, K., Raj, K.N.A., Rajeshkumar, K.C., Rajwar, S., Ralaiveloarisoa, A.B., Rämä, T., Ramírez-Cruz, V., Rambold, G., Rathnayaka, A.R., Raza, M., Ren, G.C., Rinaldi, A.C., Rivas-Ferreiro, M., Robledo, G.L., Ronikier, A., Rossi, W., Rusevska, K., Ryberg, M., Safi, A., Salimi, F., Salvador-Montoya, C.A., Samant, B., Samaradiwakara, N.P., Sánchez-Castro, I., Sandoval-Denis, M., Santiago, A.L.C.M.A., Santos, A.C.D.S., Santos, L.A. dos, Sarma, V.V., Sarwar, S., Savchenko, A., Savchenko, K., Saxena, R.K., Schoutteten, N., Selbmann, L., Ševčíková, H., Sharma, A., Shen, H.W., Shen, Y.M., Shu, Y.X., Silva, H.F., Silva-Filho, A.G.S., Silva, V.S.H., Simmons, D.R., Singh, R., Sir, E.B., Sohrabi, M., Souza, F.A., Souza-Motta, C.M., Sri-indrasutdhi, V., Sruthi, O.P., Stadler, M., Stemler, J., Stephenson, S.L., Stoyneva-Gaertner, M.P., Strassert, J.F.H., Stryjak-Bogacka, M., Su, H., Sun, Y.R., Svantesson, S., Takamatsu, S., Tan, T.H., Tanaka, K., Tang, C., Tang, X., Taylor, J.E., Taylor, P.W.J., Tennakoon, D.S., Thakshila, S.A.D., Thambugala, K.M., Thamodini, G.K., Thilanga, D., Thines, M., Tiago, P.V., Tian, X.G., Tian, W.H., Tibpromma, S., Tkalčec, Z., Tokarev, Y.S., Tomšovský, M., Torruella, G., Tsurykau, A., Udayanga, D., Ulukapı, M., Untereiner, W.A., Uzunov, B.A., Vadthanarat, S., Valenzuela, R., Van den Wyngaert, S., Van Vooren, N., Velez, P., Verma, R.K., Vieira, L.C., Vieira, W.A.S., Vinzelj, J.M., Tang, A.M.C., Walker, A., Walker, A.K., Wang, Q.M., Wang, Y., Wang, X.Y., Wang, Z.Y., Wannathes, N., Wartchow, F., Weerakoon, G., Wei, D.P., Wei, X., White, J.F., Wijesundara, D.S.A., Wisitrassameewong, K., Worobiec, G., Wu, H.X., Wu, N., Xiong, Y.R., Xu, B., Xu, J.P., Xu, R., Xu, R.F., Xu, R.J., Yadav, S., Yakovchenko, L.S., Yang, H.D., Yang, X., Yang, Y.H., Yang, Y., Yang, Y.Y., Yoshioka, R., Youssef, N.H., Yu, F.M., Yu, Z.F., Yuan, L.L., Yuan, Q., Zabin, D.A., Zamora, J.C., Zapata, C.V., Zare, R., Zeng, M., Zeng, X.Y., Zhang, J.F., Zhang, J.Y., Zhang, S., Zhang, X.C., Zhao, C.L., Zhao, H., Zhao, Q., Zhao, H., Zhao, H.J., Zhou, H.M., Zhu, X.Y., Zmitrovich, I.V., Zucconi, L. & Zvyagina, E. (2024) The 2024 Outline of Fungi and fungus-like taxa. Mycosphere 15 (1): 5146–6239. https://doi.org/10.5943/mycosphere/15/1/25
  7. Hywel-Jones, N.L. (1994) Cordyceps khaoyaiensis and C. pseudomilitaris, two new pathogens of lepidopteran larvae from Thailand. Mycological Research 98: 939–942.
  8. Index Fungorum. (2025) Available from: https://www.indexfungorum.org/Names/Names.asp (accessed 5 March 2025)
  9. Jayasiri, S.C., Hyde, K.D., Ariyawansa, H.A., Bhat, J., Buyck, B., Cai, L., Dai, Y.-C., Abd-Elsalam, K.A., Ertz, D. & Hidayat, I. (2015) The Faces of Fungi database: fungal names linked with morphology, phylogeny and human impacts. Fungal Diversity 74: 3–18. https://doi.org/10.1007/s13225-015-0351-8
  10. Jayawardena, R.S., Bhunjun, C.S., Hyde, K.D., Gentekaki, E. & Itthayakorn, P. (2021) Colletotrichum: lifestyles, biology, morpho-species, species complexes and accepted species. Mycosphere 12 (1): 519–669. https://doi.org/10.5943/mycosphere/12/1/7
  11. Jeewon, R. & Hyde, K.D. (2016) Establishing species boundaries and new taxa among fungi: recommendations to resolve taxonomic ambiguities. Mycosphere 7 (11): 1669–1677. https://doi.org/10.5943/mycosphere/7/11/4
  12. Kepler, R., Sung, G.-H., Ban, S., Nakagiri, A., Chen, M.-J., Huang, B., Li, Z.Z. & Spatafora, J. (2012) New teleomorph combinations in the entomopathogenic genus Metacordyceps. Mycologia 104 (1): 182–197. https://doi.org/10.3852/11-070
  13. Kepler, R.M., Luangsa-ard, J.J., Hywel-Jones, N.L., Quandt, C.A., Sung, G.-H., Rehner, S.A., Aime, M.C., Henkel, T.W., Sanjuan, T., Zare, R., Chen, M.J., Li, Z.Z., Rossman, A.Y., Spatafora, J.W. & Shrestha, B. (2017) A phylogenetically-based nomenclature for Cordycipitaceae (Hypocreales). IMA Fungus 8: 335–353. https://doi.org/10.5598/imafungus.2017.08.02.08
  14. Li, Y., Zhao, X.-C., Wu, L.-X., Wang, Y., Xu, A. & Lin, W.-F. (2023) Blackwellomyces kaihuaensis and Metarhizium putuoense (Hypocreales), two new entomogenous fungi from subtropical forests in Zhejiang Province, Eastern China. Forests 14 (12): 2333. https://doi.org/10.3390/f14122333
  15. Liu, Y.J., Whelen, S. & Hall, B.D. (1999) Phylogenetic relationships among ascomycetes: evidence from an RNA polymerse II subunit. Molecular Biology and Evolution 16 (12): 1799–1808. https://doi.org/10.1093/oxfordjournals.molbev.a026092
  16. Luangsa-ard, J., Houbraken, J., van Doorn, T., Hong, S.-B., Borman, A.M., Hywel-Jones, N.L. & Samson, R.A. (2011) Purpureocillium, a new genus for the medically important Paecilomyces lilacinus. FEMS Microbiology Letters 321 (2): 141–149. https://doi.org/10.1111/j.1574-6968.2011.02322.x
  17. Ma, J., Wang, Z., Yang, Z., Sun, T., Yang, S. & Yu, H. (2024) Morphology, phylogeny, and mitogenomics reveal a new entomopathogenic fungus, Blackwellomyces changningensis (Hypocreales, Clavicipitaceae), from southwestern China. Mycologia 117 (1): 166–182. https://doi.org/10.1080/00275514.2024.2399996
  18. Matheny, P.B. (2005) Improving phylogenetic inference of mushrooms with RPB1 and RPB2 nucleotide sequences (Inocybe; Agaricales). Molecular Phylogenetics and Evolution 35 (1): 1–20. https://doi.org/10.1016/j.ympev.2004.11.014
  19. Mongkolsamrit, S., Noisripoom, W., Thanakitpipattana, D., Wutikhun, T., Spatafora, J.W. & Luangsa-ard, J. (2018) Disentangling cryptic species with isaria-like morphs in Cordycipitaceae. Mycologia 110 (1): 230–257. https://doi.org/10.1080/00275514.2018.1446651
  20. Mongkolsamrit, S., Noisripoom, W., Tasanathai, K., Khonsanit, A., Thanakitpipattana, D., Himaman, W., Kobmoo, N. & Luangsa-ard, J.J. (2020) Molecular phylogeny and morphology reveal cryptic species in Blackwellomyces and Cordyceps (Cordycipitaceae) from Thailand. Mycological Progress 19: 957–983. https://doi.org/10.1007/s11557-020-01615-2
  21. Nguyen, L.-T., Schmidt, H.A., Von Haeseler, A. & Minh, B.Q. (2015) IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular Biology and Evolution 32 (1): 268–274. https://doi.org/10.1093/molbev/msu300
  22. Nylander, J. (2004) MrModeltest 2.2. Program distributed by the author. Uppsala University, Uppsala: Department of Systematic Zoology.
  23. Phutthacharoen, K., Llanos-Lopez, N.A., Toshe, R., Noisripoom, W., Khonsanit, A., Luangsa-ard, J.J., Hyde, K.D., Ebada, S.S. & Stadler, M. (2024) Bioactive bioxanthracene and cyclodepsipeptides from the entomopathogenic fungus Blackwellomyces roseostromatus BCC56290. Antibiotics (Basel) 13 (7): 585. https://doi.org/10.3390/antibiotics13070585
  24. Rehner, S.A., Minnis, A.M., Sung, G.-H., Luangsa-ard, J.J., Devotto, L. & Humber, R.A. (2011) Phylogeny and systematics of the anamorphic, entomopathogenic genus Beauveria. Mycologia 103 (5): 1055–1073. https://doi.org/10.3852/10-302
  25. Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19 (12): 1572–1574. https://doi.org/10.1093/bioinformatics/btg180
  26. Sanjuan, T., Tabima, J., Restrepo, S., Læssøe, T., Spatafora, J.W. & Franco-Molano, A.E. (2014) Entomopathogens of Amazonian stick insects and locusts are members of the Beauveria species complex (Cordyceps sensu stricto). Mycologia 106 (2): 260–275. https://doi.org/10.3852/13-020
  27. Schoch, C., Seifert, K., Huhndorf, S., Robert, V., Spouge, J., Levesque, C. & Chen, W. (2012) The internal transcribed spacer as a universal DNA barcode marker for Fungi. Fungal Barcoding Consortium. Proceedings of the National Academy of Sciences of the United States of America 109: 6241–6246. https://doi.org/10.1073/pnas.1117018109
  28. Spatafora, J., Sung, G.H., Sung, J.M., Hywel-Jones, N. & White Jr, J. (2007) Phylogenetic evidence for an animal pathogen origin of ergot and the grass endophytes. Molecular Ecology 16 (8): 1701–1711. https://doi.org/10.1111/j.1365-294X.2007.03225.x
  29. Sung, G.-H. & Spatafora, J.W. (2004) Cordyceps cardinalis sp. nov., a new species of Cordyceps with an east Asian-eastern North American distribution. Mycologia 96 (3): 658–666. https://doi.org/10.1080/15572536.2005.11832962
  30. Sung, G.-H., Spatafora, J.W., Zare, R., Hodge, K.T. & Gams, W. (2001) A revision of Verticillium sect. Prostrata. II. Phylogenetic analyses of SSU and LSU nuclear rDNA sequences from anamorphs and teleomorphs of the Clavicipitaceae. Nova Hedwigia 72 (3–4): 311–328. https://doi.org/10.1127/nova.hedwigia/72/2001/311
  31. Sung, G.-H., Hywel-Jones, N.L., Sung, J.-M., Luangsa-ard, J.J., Shrestha, B. & Spatafora, J.W. (2007a) Phylogenetic classification of Cordyceps and the clavicipitaceous fungi. Studies in Mycology 57: 5–59. https://doi.org/10.3114/sim.2007.57.01
  32. Sung, G.-H., Sung, J.-M., Hywel-Jones, N.L. & Spatafora, J.W. (2007b) A multi-gene phylogeny of Clavicipitaceae (Ascomycota, Fungi): identification of localized incongruence using a combinational bootstrap approach. Molecular Phylogenetics and Evolution 44 (3): 1204–1223. https://doi.org/10.1016/j.ympev.2007.03.011
  33. Thanakitpipattana, D., Tasanathai, K., Mongkolsamrit, S., Khonsanit, A., Lamlertthon, S. & Luangsa-ard, J. (2020) Fungal pathogens occurring on Orthopterida in Thailand. Persoonia 44 (1): 140–160. https://doi.org/10.3767/persoonia.2020.44.06
  34. Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172 (8): 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
  35. Vu, D., Groenewald, M., De Vries, M., Gehrmann, T., Stielow, B., Eberhardt, U., Al-Hatmi, A., Groenewald, J.Z., Cardinali, G., Houbraken, J., Boekhout, T., Crous, P.W., Robert, V. & Verkley, G.J.M. (2019) Large-scale generation and analysis of filamentous fungal DNA barcodes boosts coverage for kingdom fungi and reveals thresholds for fungal species and higher taxon delimitation. Studies in Mycology 92: 135–154. https://doi.org/10.1016/j.simyco.2018.05.001
  36. Wang, Y.-B., Wang, Y., Fan, Q., Duan, D.-E., Zhang, G.-D., Dai, R.-Q., Dai, Y.-D., Zeng, W.-B., Chen, Z.-H., Li, D.-D., Tang, D.-X., Xu, Z.-H., Sun, T., Nguyen, T.-T., Tran, N.-L., Dao, V.-M., Zhang, C.-M., Huang, L.-D., Liu, Y.-J., Zhang, X.-M., Yang, D.-R., Sanjuan, T., Liu, X.-Z., Yang, Z.L. & Yu, H. (2020) Multigene phylogeny of the family Cordycipitaceae (Hypocreales): new taxa and the new systematic position of the Chinese cordycipitoid fungus Paecilomyces hepiali. Fungal Diversity 103: 1–46. https://doi.org/10.1007/s13225-020-00457-3
  37. Wei, D., Gentekaki, E., Wanasinghe, D., Tang, S. & Hyde, K. (2022) Diversity, molecular dating and ancestral characters state reconstruction of entomopathogenic fungi in Hypocreales. Mycoshpere 13 (2): 281–351. https://doi.org/10.5943/mycosphere/si/1f/8
  38. White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications 18: 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1

How to Cite

Huang, S., Hyde, K.D., Xiao, Z., Yang, Y., Zhang, H.-G., Gui, Q., Kang, J.-C. & Wen, T. (2025) A novel species of Blackwellomyces in Guizhou, China. Phytotaxa 691: 113–124. https://doi.org/10.11646/phytotaxa.691.2.1