Skip to main content Skip to main navigation menu Skip to site footer
Type: Article
Published: 2025-02-11
Page range: 254-264
Abstract views: 130
PDF downloaded: 8

Morphology and phylogeny reveal a novel species, Tolypocladium phycosomatis (Ophiocordycipitaceae, Hypocreales), from Yunnan, China

Yunnan Herbal Laboratory, College of Ecology and Environmental Sciences, Yunnan University, Kunming, Yunnan 650504, China. The International Joint Research Center for Sustainable Utilization of Cordyceps Bioresources in China and Southeast Asia, Yunnan University, Kunming 650091, China.
Yunnan Herbal Laboratory, College of Ecology and Environmental Sciences, Yunnan University, Kunming, Yunnan 650504, China. The International Joint Research Center for Sustainable Utilization of Cordyceps Bioresources in China and Southeast Asia, Yunnan University, Kunming 650091, China.
Yunnan Herbal Laboratory, College of Ecology and Environmental Sciences, Yunnan University, Kunming, Yunnan 650504, China. The International Joint Research Center for Sustainable Utilization of Cordyceps Bioresources in China and Southeast Asia, Yunnan University, Kunming 650091, China.
Yunnan Herbal Laboratory, College of Ecology and Environmental Sciences, Yunnan University, Kunming, Yunnan 650504, China. The International Joint Research Center for Sustainable Utilization of Cordyceps Bioresources in China and Southeast Asia, Yunnan University, Kunming 650091, China.
The International Joint Research Center for Sustainable Utilization of Cordyceps Bioresources in China and Southeast Asia, Yunnan University, Kunming 650091, China
Yunnan Jinping Fenshuiling National Nature Reserve, Honghe, Yunnan 661500, China
Yunnan Herbal Laboratory, College of Ecology and Environmental Sciences, Yunnan University, Kunming, Yunnan 650504, China. The International Joint Research Center for Sustainable Utilization of Cordyceps Bioresources in China and Southeast Asia, Yunnan University, Kunming 650091, China.
New taxa phylogenetic analysis morphology Tolypocladium taxonomy Fungi

Abstract

Tolypocladium is classified within family Ophiocordycipitaceae. We formally describe and illustrate a novel species, Tolypocladium phycosomatis using morphological features and phylogenetic analyses. This new species was discovered in Yunnan Province, China. Phylogenetic analysis based on ITS, nrSSU, nrLSU, tef-1α, rpb1, and rpb2 gene sequences revealed that this species clustered closely with T. ovalisporum, forming a sister relationship. A comprehensive phylogenetic tree of the genus Tolypocladium was reconstructed, providing a foundation for further phylogenetic studies of this genus and its related taxa. This discovery enhances our understanding of species diversity within the genus Tolypocladium and provides a robust taxonomic foundation for their rational development and sustainable utilization.

References

  1. Barron, G.L. (1981) Two new fungal parasites of bdelloid rotifers. Canadian Journal of Botany 59: 1449–1455. https://doi.org/10.1139/b81-198
  2. Barron, G.L. (1983) Structure and biology of a new Tolypocladium attacking bdelloid rotifers. Canadian Journal of Botany 61: 2566–2569. https://doi.org/10.1139/b83-281
  3. Bills, G.F., Polishook, J.D., Goetz, M.A., Sullivan, R.F. & White, J.F. (2002) Chaunopycnis pustulata sp. nov., a new clavicipitalean anamorph producing metabolites that modulate potassium ion channels. Mycological Progress 1: 3–17. https://doi.org/10.1007/s11557-006-0001-3
  4. Bischoff, J.F., Rehner, S.A. & Humber, R.A. (2006) Metarhizium frigidum sp. nov.: a cryptic species of M. anisopliae and a member of the M. flavoviride complex. Mycologia 98 (5): 737–745. https://doi.org/10.3852/mycologia.98.5.737
  5. Bissett, J. (1983) Notes on Tolypocladium and related genera. Canadian Journal of Botany 61: 1311–1329. https://doi.org/10.1139/b83-139
  6. Crous, P.W., Wingfeld, M.J., Burgess, T.I., Hardy, G.E.St.J., Barber, P.A., Alvarado, P., Barnes, C.W., Buchanan, P.K., Heykoop, M., Moreno, G., Thangavel, R., Van der Spuy S, Barili, A., Barrett, S., Cacciola, S.O., Cano-Lira, J.F., Crane, C., Decock, C., Gibertoni, T.B., Guarro, J., Guevara-Suarez, M., Hubka, V., Kolařík, M., Lira, C.R.S., Ordoñez, M.E., Padamsee, M., Ryvarden, L., Soares, A.M., Stchigel, A.M., Sutton, D.A., Vizzini, A., Weir, B.S., Acharya, K., Aloi, F., Baseia, I.G., Blanchette, R.A., Bordallo, J.J., Bratek, Z., Butler, T., Cano-Canals, J., Carlavilla, J.R., Chander, J., Cheewangkoon, R., Cruz, R.H.S.F., Da Silva, M., Dutta, A.K., Ercole, E., Escobio, V., Esteve-Raventós, F., Flores, J.A., Gené, J., Góis, J.S., Haines, L., Held, B.W., Horta Jung, M., Hosaka, K., Jung, T., Jurjević, Ž., Kautman, V., Kautmanova, I., Kiyashko, A.A., Kozanek, M., Kubátová, A., Lafourcade, M., La Spade, F., Latha, K.P.D., Madrid, H., Malysheva, E.F., Manimohan, P., Manjón, J.L., Martín, M.P., Mata, M., Merényi, Z., Morte, A., Nagy, I., Normand, A.C., Paloi, S., Pattison, N., Pawłowska, J., Pereira, O.L., Petterson, M.E., Picillo, B., Raj, K.N.A., Roberts, A., Rodríguez, A., Rodríguez-Campo, F.J., Romański, M., Ruszkiewicz-Michalska, M., Scanu, B., Schena, L., Semelbauer, M., Sharma, R., Shouche, Y.S., Silva, V., Staniaszek-Kik, M., Stielow, J.B., Tapia, C., Taylor, P.W.J., Toome-Heller, M., Vabeikhokhei, J.M.C., Van Diepeningen, A.D, Van Hoa, N., Van Tri, M., Wiederhold, N.P., Wrzosek, M., Zothanzama, J. & Groenewald, J.Z. (2017) Fungal Planet description sheets: 558–624. Persoonia 38: 240–384. https://doi.org/10.3767/003158517X698941
  7. Das, K., Ryu, J.J., Hong, S.M., Lim, S.K., Lee, S.Y. & Jung, H.Y. (2023) Molecular phylogeny and morphology of Tolypocladium globosum sp. nov. isolated from soil in Korea. Mycobiology 51 (2): 79–86. https://doi.org/10.1080/12298093.2023.2192614
  8. Dong, Q.Y., Wang, Y., Wang, Z.Q., Liu, Y.F. & Yu, H. (2022) Phylogeny and systematics of the genus Tolypocladium (Ophiocordycipitaceae, Hypocreales). Journal of Fungi 8 (11): 1158. https://doi.org/10.3390/jof8111158
  9. Dowsett, J.A., Reid, J. & Hopkin, A. (1982) On Cephalosporium Balanoides Drechsler. Mycologia 74 (4): 687–690. https://doi.org/10.1080/00275514.1982.12021571
  10. Gams, W. (1971) Tolypocladium, eine Hyphomycetengattung mit geschwollenen Phialiden. Persoonia 6: 185–191.
  11. Gams, W. (1980) Chaunopycnis alba, gen. et sp. nov. a soil fungus intermediate between moniliales and sphaeropsidales. Persoonia 11: 75–79.
  12. Gazis, R., Skaltsas, D. & Chaverri, P. (2014) Novel endophytic lineages of Tolypocladium provide new insights into the ecology and evolution of Cordyceps-like fungi. Mycologia 106: 1090–1105. https://doi.org/10.3852/13-346
  13. Hodge, K.T., Krasnof, S.B. & Humber, R.A. (1996) Tolypocladium infatum is the anamorph of Cordyceps subsessilis. Mycologia 88: 715– 719. https://doi.org/10.1080/00275514.1996.12026708
  14. Kalyaanamoorthy, S., Minh, B.Q., Wong, T.K.F., von Haeseler, A. & Jermiin, L.S. (2017) ModelFinder: fast model selection for accurate phylogenetic estimates. Nature Methods 14: 587–589. https://doi.org/10.1038/nmeth.4285
  15. Kobayasi, Y. (1978) Cordyceps species from Japan. Bulletin of the National Science Museum Series B: Botany 4 (2): 43–63.
  16. Kobayasi, Y. & Shimizu, D. (1963) Monographic Studies of Cordyceps 2. Group parasitic on Cicadidae. Bulletin of the National Science Museum 6 (3): 286–314.
  17. Li, C.L. (1986) Study of a new species of Tolypocudium. Journal of Nanjing University (Natural Sciences) 22: 401–407.
  18. Li, C.R., Hywel-Jones, N., Cao, Y.P., Nam, S. & Li, Z.Z. (2018) Tolypocladium dujiaolongae sp. nov. and its allies. Mycotaxon 133: 229–241. https://doi.org/10.5248/133.229
  19. Lin, Q.Y. (2008) Study on the identification and biology of Cordyceps guangdongensis. South China Botanical Garden. Chinese Academy of Sciences, 32 pp.
  20. Liu, Y.F. (2020) Studies on phylogeny and mitogenomes of the genus Tolypocladium. Yunnan University. https://doi.org/10.27456/d.cnki.gyndu.2020.002854
  21. Liu, Z.Y., Liang, Z.Q., Whalley, A.J.S., Yao, Y.J. & Liu, A.Y. (2001) Cordyceps brittlebankisoides, a new pathogen of grubs and its anamorph, Metarhizium anisopliae var. majus. Journal of Invertebrate Pathology 78: 178–182. https://doi.org/10.1006/jipa.2001.5039
  22. Mains, E. (1957) Species of Cordyceps parasitic on Elaphomyces. Bulletin of the Torrey Botanical Club 84: 243. https://doi.org/10.2307/2482671
  23. Matha, V., Jegorov, A., Weiser, J. & Pillai, J.S. (1992) The mosquitocidal activity of conidia of Tolypocladium tundrense and Tolypocladium terricola. Cytobios 69: 163–170.
  24. Quandt, C.A., Kepler, R.M., Gams, W., Araújo, J.P.M., Ban, S., Evans, H.C., Hughes, D., Humber, R., Hywel-Jones, N., Li, Z.Z., Luangsa-ard, J.J., Rehner, S.A., Sanjuan, T., Sato, H., Shrestha, B., Sung, G.H., Yao, Y.J., Zare, R. & Spatafora, J.W. (2014) Phylogenetic-based nomenclatural proposals for Ophiocordycipitaceae (Hypocreales) with new combinations in Tolypocladium. IMA Fungus 5: 121–134. https://doi.org/10.5598/imafungus.2014.05.01.12
  25. Rehner, S.A. & Samuels, G.J. (1994) Taxonomy and phylogeny of Gliocladium analysed from nuclear large subunit ribosomal DNA sequences. Mycological Research 98 (6): 625–634. https://doi.org/10.1016/S0953-7562(09)80409-7
  26. Samson, R.A. & Soares, G.G. (1984) Entomopathogenic species of the hyphomycete genus Tolypocladium. Journal of Invertebrate Pathology 43: 133–139. https://doi.org/10.1016/0022-2011(84)90130-7
  27. Sung, G.H., Hywel-Jones, N.L., Sung, J.M., Luangsa-ard, J.J., Shrestha, B. & Spatafora, J.W. (2007) Phylogenetic classification of Cordyceps and the clavicipitaceous fungi. Studies in Mycology 57: 5–59. https://doi.org/10.3114/sim.2007.57.01
  28. Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172 (8): 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
  29. Wang, Y., Liu, Y.F., Tang, D.X., Wang, Y.B., Adams, A.E.M. & Yu, H. (2022) Tolypocladium reniformisporum sp. nov. and Tolypocladium cylindrosporum (Ophiocordycipitaceae, Hypocreales) co-occurring on Ophiocordyceps sinensis. Mycological Progress 21: 199–214. https://doi.org/10.1007/s11557-021-01675-y
  30. Wang, Y.B., Wang, Y., Fan, Q., Duan, D.E., Zhang, G.D., Dai, R.Q., Dai, Y.D., Zeng, W.B., Chen, Z.H., Li, D.D., Tang, D.X., Xu, Z.H., Sun, T., Nguyen, T.T., Tran, N.L., Dao, V.M., Zhang, C.M., Huang, L.D., Liu, Y.J., Zhang, X.M., Yang, D.R., Sanjuan, T., Liu, X.Z., Yang, Z.L. & Yu, H. (2020) Multigene phylogeny of the family Cordycipitaceae (Hypocreales): new taxa and the new systematic position of the Chinese cordycipitoid fungus Paecilomyces hepiali. Fungal Diversity 103: 1–46. https://doi.org/10.1007/s13225-020-00457-3
  31. Wang, Y.B., Yu, H., Dai, Y.D., Wu, C.K. & Liang, Z.Q. (2015) Polycephalomyces agaricus, a new hyperparasite of Ophiocordyceps sp. infecting melolonthid larvae in southwestern China. Mycological Progress 14 (9). https://doi.org/10.1007/s11557-015-1090-7
  32. Weiser, J., Matha, V. & Jegorov, A. (1991) Tolypocladium terricola sp. n., a new mosquito-killing species of the genus Tolypocladium Gams (Hyphomycetes). Folia Parasit 38: 363–369.
  33. White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR protocols: a guide to methods and applications. Academic Press, San Diego, pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
  34. Yu, F.M., Thilini Chethana, K.W., Wei, D.P., Liu, J.W., Zhao, Q., Tang, S.M., Li, L. & Hyde, K.D. (2021) Comprehensive review of Tolypocladium and description of a novel lineage from Southwest China. Pathogens 10: 1389. https://doi.org/10.3390/pathogens10111389
  35. Zhang, D., Gao, F., Jakovlić, I., Zou, H., Zhang, J., Li, W.X. & Wang, G.T. (2020) PhyloSuite: an integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies. Molecular Ecology Resources 20 (1): 348–355. https://doi.org/10.1111/1755-0998.13096
  36. Zhang, X.Y. (2015) A study on biology of Tolypocladium ophioglossides and its relative species. Master Dissertation, Yunnan University, China.

How to Cite

Li, S.-Y., Lu, Y.-L., Zhao, J., Ma, J.-M., Yang, S.-D., Yu, Z.-Y. & Yu, H. (2025) Morphology and phylogeny reveal a novel species, Tolypocladium phycosomatis (Ophiocordycipitaceae, Hypocreales), from Yunnan, China. Phytotaxa 684 (2): 254–264. https://doi.org/10.11646/phytotaxa.684.2.8