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Type: Article
Published: 2025-02-20
Page range: 27-39
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Molecular phylogeny and morphology reveal a new species of Exidia (Auriculariaceae, Auriculariales) from Yunnan Province, China

Department of Edible Fungi, Institute of Biology, Gansu Academy of Sciences, Lanzhou 730000, P.R. China. College of Horticulture, Gansu Agricultural University, Lanzhou 730000, P.R. China.
College of Forestry, Southwest Forestry University, Kunming 650224, P.R. China
Yunnan Wumeng Mountains National Nature Reserve, Zhaotong 657000, P.R. China
Yunnan Key Laboratory of Gastrodia and Fungal Symbiotic Biology, Zhaotong University, Zhaotong 657000, P.R. China
College of Forestry, Southwest Forestry University, Kunming 650224, P.R. China
Department of Edible Fungi, Institute of Biology, Gansu Academy of Sciences, Lanzhou 730000, P.R. China. College of Horticulture, Gansu Agricultural University, Lanzhou 730000, P.R. China.
College of Forestry, Southwest Forestry University, Kunming 650224, P.R. China. Yunnan Key Laboratory of Gastrodia and Fungal Symbiotic Biology, Zhaotong University, Zhaotong 657000, P.R. China.
Biodiversity corticioid fungi molecular systematic taxonomy Fungi

Abstract

In this study, Exidia punctata is described and illustrated as a novel species based on morphological characteristics and phylogenetic analyses of internal transcribed spacer (ITS) regions and the large subunit (nLSU) of nuclear ribosomal RNA genes. The species is characterized by smooth, membranaceous, cushion-shaped basidiomata with white to cream hymenial surface, and narrowly cylindrical to allantoid basidiospores (13–15.5 × 5–6.5 µm). The phylogenetic tree inferred from ITS+nLSU sequences revealed that the new species was nested into the genus Exidia within the family Auriculariaceae, in which it grouped with the clade comprising E. glandulosa, E. pithya, E. reflexa and E. subglandulosa.

References

  1. Alvarenga, R.L.M. & Gibertoni, T.B. (2021) Crystallodon Alvarenga gen. nov., a new genus of the Auriculariales from the Neotropics. Cryptogamie Mycologie 42: 17–24. https://doi.org/10.5252/cryptogamie-mycologie2021v42a2
  2. Alvarenga, R.L.M., Spirin, V., Malysheva, V., Gibertoni, T.B. & Larsson, K.H. (2019) Two new genera and six other novelties in Heterochaete sensu lato (Auriculariales, Basidiomycota). Botany 97: 439–451. http://doi.org/10.1139/cjb-2019-0046
  3. Bian, L.S., An, Q., Wang, X.H., Chen, L. & Han, M.L. (2023) Diversity of wood-inhabiting macrofungi in the Taihang Mountains, northern China. Mycosystema 42 (11): 2188–2202. http://doi.org/10.13346/j.mycosystema.230088
  4. Deng, Y.L., Chen, M., Liu, L.F., Zhao, R., Li, Q., Miao, J.Y., Zhao, C.L. & Zhou, H.M. (2024) Molecular phylogeny and morphology reveal a new Eichleriella species (Auriculariales, Basidiomycota) from China. Phytotaxa 675 (3): 247–260. https://doi.org/10.11646/phytotaxa.675.3.4
  5. Dong, J.H., Li, Q., Yuan, Q., Luo, Y.X., Zhang, X.C., Dai, Y.F., Zhou, Q., Liu, X.F., Deng, Y.L., Zhou, H.M., Zhao, C.L. & Muhammad, A. (2024a) Species diversity, taxonomy, molecular systematics and divergence time of wood-inhabiting fungi in Yunnan-Guizhou Plateau, Asia. Mycosphere 15 (1): 1110–1293. https://doi.org/10.5943/mycosphere/15/1/10
  6. Dong, J.H., Zhu, Y.G., Qian, C.B. & Zhao, C.L. (2024b) Taxonomy and phylogeny of Auriculariales (Agaricomycetes, Basidiomycota) with descriptions of four new species from southwestern China. MycoKeys 108: 115–146. https://doi.org/10.3897/mycokeys.108.128659
  7. Felsenstein, J. (1985) Confidence intervals on phylogenetics: an approach using bootstrap. Evolution 39: 783–791. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x
  8. Fries, E.M. (1822) Systema Mycologicum, vol. 2 (1). Lundae, Ex Officina Berlingiana, pp. 1–274.
  9. Guan, Q.X., Liu, C.M., Zhao, T.J. & Zhao, C.L. (2020) Heteroradulum yunnanensis sp. nov. (Auriculariales, Basidiomycota) evidenced by morphological characters and phylogenetic analyses in China. Phytotaxa 437: 51–59. https://doi.org/10.11646/phytotaxa.437.2.1
  10. He, M.Q., Cao, B., Liu, F., Boekhout, T., Denchev, T.T., Power, N., Denchev, C.M., Kemler, M., Gorjón, S.P., Begerow, D., Valenzuela, R., Davoodian, N., Niskanen, T., Vizzini, A., Redhead, S.A., RamírezCruz, V., Papp, V., Dudka, V.A., Dutta, A.K., García-Sandoval, R., Liu, X.Z., Kijpornyongpan, T., Savchenko, A., Tedersoo, L., Theelen, B., Trierveiler-Pereira, L., Wu, F., Zamora, J.C., Zeng, X.Y., Zhou, L.W., Liu, S.L., Ghobad-Nejhad, M., Giachini, A.J., Li, G.J., Kakishima, M., Olariaga, I., Haelewaters, D., Sulistyo, B., Sugiyama, J., Svantesson, S., Yurkov, A., Alvarado, P., Antonín, V., da Silva, A.F., Druzhinina, I., Gibertoni, T.B., Guzmán, D.L., Justo, A., Karunarathna, S.C., Galappaththi, M.C.A., Toome-Heller, M., Hosoya, T., Liimatainen, K., Márquez, R., Mešić, A., Moncalvo, J.M., Nagy, L.C., Varga, T., Orihara, T., Raymundo, T., Salcedo, I., Silva-Filho, A.G.S., Tkalčec, Z., Wartchow, F., Zhao, C.L., Bau, T., Cabarroi-Hernández, M., Cortés, P.A., Decock, C., De Lange, R., Weiss, M., Menolli, N., Nilsson, R.H., Fan, Y.G., Verbeken, A., Gaforov, Y., Meiras-Ottoni, A., Mendes-Alvarenga, R.L., Zeng, N.K., Wu, Q., Hyde, K.D., Kirk, P.M. & Zhao, R.L. (2024) Phylogenomics, divergence times and notes of orders in Basidiomycota. Fungal Diversity 126 (3): 1–280. https://doi.org/10.1007/s13225-024-00535-W
  11. Jiang, Y.H., Wang, K., Huang, J.M., Bian, L.S. & Yuan, Y. (2024) Diversity and funga of macrofungi in Xiaolongmen National Forest Park, Beijing, North China. Mycosystema 43 (3): 230–296. https://doi.org/10.13346/j.mycosystema.230296
  12. Katoh, K., Rozewicki, J. & Yamada, K.D. (2019) MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics 20: 1160–1166. https://doi.org/10.1093/bib/bbx108
  13. Larsson, A. (2014) AliView: a fast and lightweight alignment viewer and editor for large data sets. Bioinformatics 30: 3276–3278. https://doi.org/10.1093/bioinformatics/btu531
  14. Li, Y., Nie, T., Nakasone, K.K., Li, H.J. & He, S.H. (2023) Taxonomy and phylogeny of corticioid fungi in Auriculariaceae (Auriculariales, Basidiomycota): a new genus, five new species and four new combinations. Journal of Fungi 9 (3): 318. https://doi.org/10.3390/jof9030318
  15. Liu, S.L., Shen, Z.Q., Li, Q.Z, Liu, X.Y. & Zhou, L.W (2022) Alloexidiopsis gen. nov., a revision of generic delimitation in Auriculariales (Basidiomycota). Frontiers in Microbiology 13: 894641. https://doi.org/10.3389/fmicb.2022.894641
  16. Liu, X.F., Shen, S. & Zhao, C.L. (2019) Morphological and molecular identification of a new species of Eichleriella (Auriculariales, Basidiomycota) in China. Phytotaxa 404: 245–254. http://doi.org/10.11646/phytotaxa.404.6.3
  17. Malysheva, V. (2012) A revision of the genus Exidia (Auriculariales, Basidiomycota) in Russia. Mikol Fitopatog 46: 365–376.
  18. Malysheva, V. & Spiri, V. (2017) Taxonomy and phylogeny of the Auriculariales (Agaricomycetes, Basidiomycota) with stereoid basidiomata. Fungal Biology 121: 689–715. https://doi.org/10.1016/j.funbio.2017.05.001
  19. Miettinen, O., Spirin, V. & Niemelä, T. (2012) Notes on the genus Aporpium (Auriculariales, Basidiomycota), with a new species from temperate Europe. Annales Botanici Fennici 49: 359–368. https://doi.org/10.5735/085.049.0607
  20. Miller, M.A., Pfeiffer, W. & Schwartz, T. (2012) The CIPRES science gateway. In: Proceedings of the 1st Conference of the Extreme Science and Engineering Discovery Environment: Bridging from the Extreme to the Campus and Beyond. Chicago, IL, USA, 39 pp. https://doi.org/10.1145/2335755.2335836
  21. Nylander, J.A.A. (2004) MrModeltest v2. Program distributed by the author. Evolutionary Biology Centre, Uppsala University. Ampignons de l’Équateur (Pugillus IV). Bulletin de íHerbier Boissier 3: 53–74.
  22. Petersen, J.H. (1996) Farvekort. The Danish Mycological Society’s Colour-Chart. Foreningen til Svampekundskabens Fremme: Greve, Germany, 6 pp.
  23. Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Hӧhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) Mrbayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61: 539–542. https://doi.org/10.1093/sysbio/sys029
  24. Sotome, K., Maekawa, N., Nakagiri, A., Lee, S.S. & Hattori, T. (2014) Taxonomic study of Asian species of poroid Auriculariales. Mycological Progress 13 (4): 987–997. https://doi. org/10.1007/s11557-014-0984-0
  25. Spirin, V., Malysheva, V. & Larsson, K.H. (2018) On some forgotten of Exidia and Myxarium (Auriculariales, Basidiomycota). Nordic Journal of Botany 36: e01601. https://doi. org/10.1111/njb.01601
  26. Sun, B.Y., Wu, Y.D. & Yuan, Y. (2023) Species diversity and floral characteristics of wood-inhabiting macrofungi growing on Quercus mongolica in Northeast China. Mycosystema 42 (1): 278–289. https://doi.org/10.13346/j.mycosystema.220258
  27. Swofford, D.L. (2002) PAUP*: Phylogenetic analysis using parsimony (*and Other Methods), Version 4.0b10. Sinauer Associates: Sunderland, MA, USA.
  28. Tohtirjap, A., Hou, S.X., Rivoire, B., Gates, G., Wu, F. & Dai, Y.C. (2023) Two new species of Exidia sensu lato (Auriculariales, Basidiomycota) based on morphology and DNA sequences. Frontiers in microbiology 13: 1080290. https://doi.org/10.3389/fmicb.2022.1080290
  29. Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238–4246. http://doi.org/10.1128/jb.172.8.4238-4246.1990
  30. Wang, S.R. & Thorn, R.G. (2021) Exidia qinghaiensis, a new species from China. Mycoscience 62: 212–216. http://doi.org/10.47371/mycosci.2021.03.002
  31. Weiß, M. & Oberwinkler, F. (2001) Phylogenetic relationships in Auriculariales and related groups-hypotheses derived from nuclear ribosomal DNA sequences. Mycological Research 105 (4): 403–415. https://doi.org/10.1017/S095375620100363X
  32. White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR protocols: A guide to methods and applications. Academic Press, San Diego, pp. 315–322.
  33. Wu, F., Yuan, Y., Malysheva, V.F., Du, P. & Dai, Y.C. (2014) Species clarification of the most important and cultivated Auricularia mushroom “Heimuer”: evidence from morphological and molecular data. Phytotaxa 186: 241–253. https://doi.org/10.11646/phytotaxa.186.5.1
  34. Wu, F., Yuan, Y., He, S.H., Bandara, A.R., Hyde, K.D., Malysheva, V.F. & Dai, Y.C. (2015) Global diversity and taxonomy of the Auricularia auricula-judae complex (Auriculariales, Basidiomycota). Mycological Progress 14: 95. https://doi.org/10.1007/s11557-015-1113-4
  35. Wu, F., Zhao, Q., Yang, Z.L., Ye, S.Y., Rivoire, B. & Dai, Y.C. (2020) Exidia yadongensis, a new edible species from East Asia. Mycosystema 39: 1203–1214. https://doi.org/10.13346/j.mycosystema.200205
  36. Ye, S.Y., Zhang, Y.B., Wu, F. & Liu, H.X. (2020) Multi-locus phylogeny reveals two new species of Exidia (Auriculariales, Basidiomycota) from China. Mycological Progress 19: 859–868. https://doi.org/10.1007/s11557-020-01601-8
  37. Yuan, Y., Bian, L.S., Wu, Y.D., Chen, J.J., Wu, F., Liu, H.G., Zeng, G.Y. & Dai, Y.C. (2023) Species diversity of pathogenic wood-rotting fungi (Agaricomycetes, Basidiomycota) in China. Mycology 14: 204–226. https://doi.org/10.1080/21501203.2023.2238779
  38. Yuan, H.S., Lu, X. & Decock, C. (2018) Molecular and morphological evidence reveal a new genus and species in Auriculariales from tropical China. MycoKeys 35: 27–39. https://doi.org/10.3897/mycokeys.35.25271
  39. Zhao, C.L., Qu, M.H., Huang, R.X. & Karunarathna, S.C. (2023) Multi-gene phylogeny and taxonomy of the wood-rotting fungal genus Phlebia sensu lato (Polyporales, Basidiomycota). Journal of Fungi 9: 320. https://doi.org/10.3390/jof9030320
  40. Zhao, C.L. & Wu, Z.Q. (2017) Ceriporiopsis kunmingensis sp. nov. (Polyporales, Basidiomycota) evidenced by morphological characters and phylogenetic analysis. Mycological Progress 16: 93–100. https://doi.org/10.1007/s11557-016-1259-8
  41. Zhu, A.H., Zhang, Q.Y., Luo, K.Y., He, S.H. & Ma, H.X. (2024) Diversity of wood-inhabiting macrofungi in tropical areas of Yunnan Province, China. Mycosystema 43 (3): 230265.

How to Cite

Jiang, Y., Wang, L., Zhou, C., Shen, K., Zhang, S., Yang, A. & Zhao, C. (2025) Molecular phylogeny and morphology reveal a new species of Exidia (Auriculariaceae, Auriculariales) from Yunnan Province, China. Phytotaxa 689 (1): 27–39. https://doi.org/10.11646/phytotaxa.689.1.3