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Issue: Vol. 684 No. 2: 11 Feb. 2025
Type: Article
Published: 2025-02-11
DOI: 10.11646/phytotaxa.684.2.5
Page range: 219-231
Abstract views: 132
PDF downloaded: 6

Two new species of Helvella (Helvellaceae, Pezizales) from Southwestern China

State Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China
State Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China. Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand. School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand.
State Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China
State Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China. Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand. School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand.
State Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China. Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand. School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand.
Management Bureau of Jinguangsi Provincial Nature Reserve, Dali 672600, Yunnan, China
State Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China
State Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China
Helvellaceae morphology multi-locus analysis new taxa phylogeny Fungi

Abstract

Species of Helvella are widely distributed in the northern temperate zone, and there are currently at least 96 phylogenetic species in China. During an investigation of discomycetous diversity in Yunnan, China, four specimens of Helvella were collected and identified as two new species, Helvella kunmingensis and H. yongpingensis, based on morphological and molecular evidences. They are similar in cupulate to discoid, greyish black apothecium, with pubescence on the receptacle surface and a developed stipe. The asci of H. kunmingensis is narrower than that of H. yongpingensis. Phylogenetic analyses of multi-locus (ITS, nrLSU, tef-1α, and hsp90) indicated that they were not related, and H. yongpingensis was closely related to H. xiaohuipan, while H. kunmingensis was sister to H. orentitomentosa. Two new species are described, illustrated, and compared with the sibling and morphologically similar species in this paper.

References

  1. Abbott, S.P. & Currah, R.S. (1997) The Helvellaceae: systematic revision and occurrence in northern and northwestern North America. Mycotaxon 62: 1–125.
  2. Bao, S.X., Li, Y., Zhou, D.Q., Thongklang, N., Yu, F.M. & Zhao, Q. (2023) Two new species of the fallax-pezizoides complex in Helvella (Helvellaceae, Pezizales) from Yunnan, China. Phytotaxa 626: 279–289. https://doi.org/10.11646/phytotaxa.626.4.5
  3. Capella-Gutiérrez, S., Silla-Martínez, J.M. & Gabaldón, T. (2009) trimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses. Bioinformatics 25: 1972–1973. https://doi.org/10.1093/bioinformatics/btp348
  4. Dissing, H. & Nannfeldt, J.A. (1966) Helvella cupuliformis sp. nov., H. villosa (Hedw. Ex O. Kuntze) comb. nov., H. macropus (Pers. Ex Fr.) Karst., and their allies. Svensk Botanisk Tidskrift 60: 325–337.
  5. Hansen, K. & Pfister, D.H. (2006) Systematics of the Pezizomycetes—the operculate discomycetes. Mycologia 98: 1029–1040. https://doi.org/10.1080/15572536.2006.11832631
  6. Hansen, K., Schumacher, T., Skrede, I., Huhtinen, S. & Wang, X.H. (2019) Pindara revisited–evolution and generic limits in Helvellaceae. Persoonia-Molecular Phylogeny and Evolution of Fungi 42: 186–204. https://doi.org/10.3767/persoonia.2019.42.07
  7. Index Fungorum (2023) Available from: http://www.indexfungorum.org/names/Names.asp (accessed 6 December 2023)
  8. Katoh, K., Rozewicki, J. & Yamada, K.D. (2019) MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics 20: 1160–1166. https://doi.org/10.1093/bib/bbx108
  9. Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular biology and evolution 30: 772–780. https://doi.org/10.1093/molbev/mst010
  10. Landeros, F., Ferrusca-Rico, F.M., Guzman-Davalos, L., Esquivel-Naranjo, E.U., Matias-Ferrer, N., Burrola-Aguilar, C., Artemisa Viurcos-Martinez, G. & Garibay-Orijel, R. (2021) Helvella jocatoi sp. nov. (Pezizales, Ascomycota), a new species from H. lacunosa complex with cultural importance in central Mexico Abies religiosa forests. Phytotaxa 498 (1): 1–11. https://doi.org/10.11646/phytotaxa.498.1.1
  11. Landeros, F., Iturriaga, T. & Guzmán-Dávalos, L. (2012) Type studies in Helvella (Pezizales) 1. Mycotaxon 119: 35–63. https://doi.org/10.5248/119.35
  12. Landeros, F., Iturriaga, T., Rodríguez, A., Vargas-Amado, G. & Guzmán-Dávalos, L. (2015) Advances in the phylogeny of Helvella (Fungi: Ascomycota), inferred from nuclear ribosomal LSU sequences and morphological data. Revista mexicana de biodiversidad 86: 856–871. https://doi.org/10.1016/j.rmb.2015.09.005
  13. Landvik, S., Kristiansen, R. & Schumacher, T. (1999) Pindara: a miniature Helvella. Mycologia 91: 278–285. https://doi.org/10.1080/00275514.1999.12061018
  14. Li, T.S., Zhou, D.Q., Zhao, Q. & Zhang, Y. (2023) A new addition to the Helvella macropus group (Helvellaceae) from southwestern China. Phytotaxa 613: 039–046. https://doi.org/10.11646/phytotaxa.613.1.3
  15. Linnaeus, C. (1753) Species Plantarum. Vol. 2. pp. 561–1200.
  16. Liu, B., Du, F. & Cao, J.Z. (1985) New species and new combinations of the genus Helvella. Acta Mycologica Sinica 4: 208–217.
  17. Lu, J.R., Yu, F.M., Lei, L., Zhang, Y. & Zhao, Q. (2023) A new species of Helvella (Helvellaceae, Pezizomycetes) within the H. macropus group from China. Phytotaxa 601: 212–222. https://doi.org/10.11646/phytotaxa.601.3.2
  18. Løken, S.B., Skrede, I. & Schumacher, T. (2020) The Helvella corium species aggregate in Nordic countries–phylogeny and species delimitation. Fungal systematics and evolution 5: 169–186. https://doi.org/10.3114/fuse.2020.05.11
  19. Mao, N., Xu, Y.Y., Zhang, Y.X., Huang, X.B., Hou, C.L. & Fan, L. (2024) Phylogeny and species diversity of the genus Helvella with emphasis on eighteen new species from China. Fungal Systematics and Evolution 12: 111–152. https://doi.org/10.3114/fuse.2023.12.08
  20. Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: 2010 gateway computing environments workshop (GCE). pp. 1–8. https://doi.org/10.1109/GCE.2010.5676129
  21. Nguyen, N.H., Landeros, F., Garibay-Orijel, R., Hansen, K. & Vellinga, E.C. (2013) The Helvella lacunosa species complex in western North America: cryptic species, misapplied names and parasites. Mycologia 105: 1275–1286. https://doi.org/10.3852/12-391
  22. Nylander, J.A., Ronquist, F., Huelsenbeck, J.P. & Nieves-Aldrey, J. (2004) Bayesian phylogenetic analysis of combined data. Systematic biology 53: 47–67. https://doi.org/10.1080/10635150490264699
  23. Rehner, S.A. & Buckley, E. (2005) A Beauveria phylogeny inferred from nuclear ITS and EF1-α sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 97: 84–98. https://doi.org/10.1080/15572536.2006.11832842
  24. Schoch, C.L., Seifert, K.A., Huhndorf, S., Robert, V., Spouge, J.L., Levesque, C.A., Chen, W., Fungal Barcoding Consortium, Fungal Barcoding Consortium Author List, Bolchacova, E., Voigt, K., Crous, P.W., Miller, A.N., Wingfield, M.J., Aime, M.C., An, K.-D., Bai, F.-Y., Barreto, R.W., Begerow, D., Bergeron, M.-J., Blackwell, M., Boekhout, T., Bogale, M., Boonyuen, N., Burgaz, A.R., Buyck, B., Cai, L., Cai, Q., Cardinali, G., Chaverri, P., Coppins, B.J., Crespo, A., Cubas, P., Cummings, C., Damm, U., de Beer, Z.W., de Hoog, G.S., Del-Prado, R., Dentinger, B., Diéguez-Uribeondo, J., Divakar, P.K., Douglas, B., Dueñas, M., Duong, T.A., Eberhardt, U., Edwards, J.E., Elshahed, M.S., Fliegerova, K., Furtado, M., García, M.A., Ge, Z.-W., Griffith, G.W., Griffiths, K., Groenewald, J.Z., Groenewald, M., Grube, M., Gryzenhout, M., Guo, L.-D., Hagen, F., Hambleton, S., Hamelin, R.C., Hansen, K., Harrold, P., Heller, G., Herrera, C., Hirayama, K., Hirooka, Y., Ho, H.-M., Hoffmann, K., Hofstetter, V., Högnabba, F., Hollingsworth, P.M., Hong, S.-B., Hosaka, K., Houbraken, J., Hughes, K., Huhtinen, S., Hyde, K.D., James, T., Johnson, E.M., Johnson, J.E., Johnston, P.R., Jones, E.B.G., Kelly, L.J., Kirk, P.M., Knapp, D.G., Kõljalg, U., Kovács, G.M., Kurtzman, C.P., Landvik, S., Leavitt, S.D., Liggenstoffer, A.S., Liimatainen, K., Lombard, L., Luangsa-ard, J.J., Lumbsch, H.T., Maganti, H., Maharachchikumbura, S.S.N., Martin, M.P., May, T.W., McTaggart, A.R., Methven, A.S., Meyer, W., Moncalvo, J.-M., Mongkolsamrit, S., Nagy, L.G., Nilsson, R.H., Niskanen, T., Nyilasi, I., Okada, G., Okane, I., Olariaga, I., Otte, J., Papp, T., Park, D., Petkovits, T., Pino-Bodas, R., Quaedvlieg, W., Raja, H.A., Redecker, D., Rintoul, T.L., Ruibal, C., Sarmiento-Ramírez, J.M., Schmitt, I., Schüßler, A., Shearer, C., Sotome, K., Stefani, F.O.P., Stenroos, S., Stielow, B., Stockinger, H., Suetrong, S., Suh, S.-O., Sung, G.-H., Suzuki, M., Tanaka, K., Tedersoo, L., Telleria, M.T., Tretter, E., Untereiner, W.A., Urbina, H., Vágvölgyi, C., Vialle, A., Vu, T.D., Walther, G., Wang, Q.-M., Wang, Y., Weir, B.S., Weiß, M., White, M.M., Xu, J., Yahr, R., Yang, Z.L., Yurkov, A., Zamora, J.-C., Zhang, N., Zhuang, W.-Y. & Schindel, D. (2012) Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proceedings of the national academy of Sciences 109: 6241–6246. https://doi.org/10.1073/pnas.1117018109
  25. Shameem, N., Kamili, A.N., Ahmad, M., Masoodi, F.A. & Parray, J.A. (2016) Antioxidant potential and DNA damage protection by the slate grey saddle mushroom, Helvella lacunosa (Ascomycetes), from Kashmir Himalaya (India). International Journal of Medicinal Mushrooms 18: 631–636. https://doi.org/10.1615/IntJMedMushrooms.v18.i7.80
  26. Skrede, I., Carlsen, T. & Schumacher, T. (2017) A synopsis of the saddle fungi (Helvella: Ascomycota) in Europe–species delimitation, taxonomy and typification. Persoonia-Molecular Phylogeny and Evolution of Fungi 39: 201–253. https://doi.org/10.3767/persoonia.2017.39.09
  27. Skrede, I., Løken, S.B., Mathiesen, C. & Schumacher, T. (2023) Additions to the knowledge of the genus Helvella in Europe. New records and de novo description of five species from the Nordic region. Fungal Systematics and Evolution 11: 71–84. https://doi.org/10.3114/fuse.2023.11.06
  28. Vaidya, G., Lohman, D.J. & Meier, R. (2011) SequenceMatrix: concatenation software for the fast assembly of multi-gene datasets with character set and codon information. Cladistics 27: 171–180. https://doi.org/10.1111/j.1096-0031.2010.00329.x
  29. Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
  30. Wang, M., Zhao, Y.C., Zhao, Q. & Zhou, D.Q. (2016) Helvella sublactea sp. nov. (Helvellaceae) from southwestern China. Phytotaxa 253: 131–138. https://doi.org/10.11646/phytotaxa.253.2.2
  31. Wang, X.C., Liu, T.Z., Chen, S.L., Li, Y. & Zhuang, W.Y. (2019) A four-locus phylogeny of rib-stiped cupulate species of Helvella (Helvellaceae, Pezizales) with discovery of three new species. MycoKeys 60: 45–67. https://doi.org/10.3897/mycokeys.60.38186
  32. Wang, X.C., Zhuang, W.Y. & Zhao, R.L. (2023) Species diversity of Helvella lacunosa clade (Pezizales, Ascomycota) in China and description of sixteen new species. Journal of Fungi 9: 697. https://doi.org/10.3390/jof9070697
  33. White, T.J., Bruns, T., Lee, S.J.W.T. & Taylor, J.W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications 18: 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
  34. Xu, R.J., Li, L. & Zhao, Q. (2022) Helvella cystidiata sp. nov. (Helvellaceae, Ascomycota) from Tibetan Plateau, China. Phytotaxa 560: 82–92. https://doi.org/10.11646/phytotaxa.560.1.6
  35. Yu, F.M., Lei, L., Luangharn, T., Zhao, Q. & Zhu, Y.A. (2023) Four new additions to Helvella (Helvellaceae, Pezizales) from Northern Thailand. Front in Microbiology 14: 1182025. https://doi.org/10.3389/fmicb.2023.1182025
  36. Zhao, Q., Sulayman, M., Zhu, X.T., Zhao, Y.C., Yang, Z.L. & Hyde, K.D. (2016b) Species clarification of the culinary Bachu mushroom in western China. Mycologia 108: 828–836. https://doi.org/10.3852/16-002
  37. Zhao, Q., Tolgor, B., Zhao, Y., Yang, Z.L. & Hyde, K.D. (2015) Species diversity within the Helvella Crispa group (Ascomycota: Helvellaceae) in China. Phytotaxa 239: 130–142. https://doi.org/10.11646/phytotaxa.239.2.2
  38. Zhao, Q., Zhang, X., Li, S., Chai, H., Bahkali, A.H. & Hyde, K.D. (2016a) New species and records of saddle fungi (Helvella, Helvellaceae) from Jiuzhaigou Natural Reserve, China. Mycoscience 57: 422–430. https://doi.org/10.1016/j.myc.2016.07.005

How to Cite

Li, T.-S., Yang, Y.-Y., Yu, F.-M., Li, C.-J.-Y., Xu, R.-J., Yang, H.-H., Zhou, D.-Q. & Zhao, Q. (2025) Two new species of Helvella (Helvellaceae, Pezizales) from Southwestern China. Phytotaxa 684 (2): 219–231. https://doi.org/10.11646/phytotaxa.684.2.5