Skip to main content Skip to main navigation menu Skip to site footer
Responsive image
Issue: Vol. 690 No. 1: 26 Feb. 2025
Type: Article
Published: 2025-02-26
DOI: 10.11646/phytotaxa.690.1.6
Page range: 77-88
Abstract views: 103
PDF downloaded: 3

Inosperma pakistanicum sp. nov. (Inocybaceae, Agaricales) from Swat, Pakistan

Department of Botany, Division of Science & Technology, University of Education, Lahore, 54500, Pakistan
Department of Botany, Division of Science & Technology, University of Education, Lahore, 54500, Pakistan
Hazara University, Mansehra, Khyber Pakhtunkhwa, 21120, Pakistan
Basidiomycota Agaricales new species systematics Inocybaceae ectomycorrhizae phylogenetic analyses taxonomy Fungi

Abstract

Inosperma pakistanicum is described as a new species from the Swat District, Khyber Pakhtunkhwa, Pakistan. Morphological data and molecular phylogenetic analyses based on nrITS and nLSU sequences supported the fact that the species is a unique new species. Inosperma pakistanicum is characterized by campanulate to subumbonate and orange to bright brown pileus, subventricose and vivid purple to vivid reddish orange lamellae, abruptly bulbous and white to dark reddish brown stipe, broadly ellipsoid to elongate, smooth basidiospores (on average 9.79 × 6.60 μm), clavate basidia, narrowly utriform, few with median constriction cheilocystidia, oblong pileocystidia and absence of pleurocystidia and caulocystidia. The new species’ description, illustrations, phylogenetic analysis results, and comparison with closely related taxa are provided. A key to the Pakistan Inosperma species is also provided.

References

  1. Aïgnon, H.L., Fan, Y.G., De Kesel, A., Bahram, M., Ryberg, M. & Yorou, N.S. (2023) A new species of Inosperma, and first record of I. afromelliolens (Inocybaceae, Fungi) from West Africa. Plos One 18 (10): e0290894. https://doi.org/10.1371/journal.pone.0290894
  2. Aïgnon, H.L., Jabeen, S., Naseer, A., Yorou, N.S. & Ryberg, M. (2021) Three new species of Inosperma (Agaricales, Inocybaceae) from Tropical Africa. MycoKeys 77: 97–116. https://doi.org/10.3897/mycokeys.77.60084
  3. Bandini, D., Oertel, B. & Eberhardt, U. (2021) Noch mehr risspilze (2): Dreizehn neue arten der familie Inocybaceae. Mycologia Bavarica 21: 27–98.
  4. Bresadola, G. (1892) Fungi Tridentini novi, vel nondum delineati, descripti, et iconibus illustrati: I.-[II.] (Vol. 2). Monauni.
  5. Cervini, M., Carbone, M. & Bizio, E. (2021) Inosperma vinaceum, una nuova specie distinta da I. rhodiolum e I. adaequatum. Rivista di Micologia 63 (3): 215–241.
  6. Cullington, P. (2015) A key to reddening species of Inocybe in Britain. Field Mycology 16 (3): 77–92. https://doi.org/10.1016/j.fldmyc.2015.06.004
  7. Deng, L.S., Kang, R., Zeng, N.K., Yu, W.J., Chang, Z., Xu, F., Deng, W.Q., Qi, L.L., Zhou, Y.L. & Fan, Y.G. (2021) Two new Inosperma (Inocybaceae) species with unexpected muscarine contents from tropical China. MycoKeys 85: 87–108. https://doi.org/10.1080/10.3897/mycokeys.85.71957
  8. Deng, L.S., Yu, W.J., Zeng, N.K., Zhang, Y.Z., Wu, X.P., Li, H.J., Xu, F. & Fan, Y.G. (2022) A new muscarine-containing Inosperma (Inocybaceae, Agaricales) species discovered from one poisoning incident occurring in tropical China. Frontiers in Microbiology 13: p.923435. https://doi.org/10.3389/fmicb.2022.923435
  9. Esteve-Raventós, F., Larsson, E., Pancorbo, F., Bizio, E., Altés, A., Turégano, Y., Moreno, G. & Olariaga, I. (2024) A Taxonomic and Phylogenetic Contribution on Inosperma Section Inosperma (Agaricales, Inocybaceae) in Europe: Calamistratum and Geraniodorum Groups. Journal of Fungi 10 (6): 374. https://doi.org/10.3390/jof10060374
  10. Gardes, M. & Bruns, T.D. (1993) ITS primers with enhanced specificity for basidiomycetes application to the identification of mycorrhizae rusts. Molecular Ecology 2: 113–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
  11. Hall, T.A. (1999) Bioedit. A user-friendly biological sequence alignment editor and analyses program for windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.
  12. Hoang, D.T., Chernomor, O., Von Haeseler, A., Minh, B.Q. & Vinh, L.S. (2018) UFBoot2: improving the ultrafast bootstrap approximation. Molecular Biology and Evolution 35 (2): 518–522. https://doi.org/10.1093/molbev/msx281
  13. Horak, E., Matheny, P.B., Desjardin, D.E. & Soytong, K. (2015) The genus Inocybe (Inocybaceae, Agaricales, Basidiomycota) in Thailand and Malaysia. Phytotaxa 230 (3): 201–238. https://doi.org/10.11646/phytotaxa.230.3.1
  14. Hu, Y., Karunarathna, S.C., Li, H., Galappaththi, M.C., Zhao, C.L., Kakumyan, P. & Mortimer, P.E. (2022) The impact of drying temperature on basidiospore size. Diversity 14 (4): 239. https://doi.org/10.3390/d14040239
  15. Kaygusuz, O. (2022) New locality record of Inosperma bongardii with notes on its taxonomy, molecular phylogeny and ecology. International Conferences on Science and Technology Natural Sciences and Technology, Budva, Montenegro.
  16. Kirk, P.M., Cannon, P.F., Minter, D.W. & Stalpers, J.A. (2008) Dictionary of the Fungi. 10th ed. Wallingford, UK: CAB International.
  17. Kropp, B.R., Matheny, P.B. & Hutchison, L.J. (2013) Inocybe section Rimosae in Utah: phylogenetic affinities and new species Mycologia 105: 728–747. https://doi.org/10.3852/12-185
  18. Larsson, E., Ryberg, M., Moreau, P.A., Mathiesen, A.D. & Jacobsson, S. (2009) Taxonomy and evolutionary relationships within species of section Rimosae (Inocybe) based on ITS, LSU and mtSSU sequence data. Persoonia 23: 86–98. https://doi.org/10.3767/003158509X475913
  19. Latha, K.P.D. & Manimohan, P. (2016) Five new species of Inocybe (Agaricales) from tropical India. Mycologia 108: 110–122. https://doi.org/10.3852/14-358
  20. Li, S.N., Xu, F., Long, P., Liu, F., Zhang, P., Fan, Y.G. & Chen, Z.H. (2022) Five new species of Inosperma from China: Morphological characteristics, phylogenetic analyses, and toxin detection. Frontiers in Microbiology 13: 1021583. https://doi.org/10.3389/fmicb.2022.1021583
  21. Matheny, P.B., Henkel, T.W., Sene, O., Korotkin, H.B, Dentinger, B.T.M. & Aime, M.C. (2017) New species of Auritella (Inocybaceae) from Cameroon, with a worldwide key to the known species. IMA Fungus 8: 287–298. https://doi.org/10.5598/imafungus.2017.08.02.06
  22. Matheny, P.B. & Kudzma, L.V. (2019) New species of Inocybe (Inocybaceae) from eastern North America 1. Journal of the Torrey Botanical Society 146 (3): 213–235. https://doi.org/10.3159/TORREY-D-18-00060.1
  23. Matheny, P.B. (2005) Improving phylogenetic inference of mushrooms with RPB1 and RPB2 nucleotide sequences (Inocybe; Agaricales). Molecular Phylogenetic Evolution 35: 1–20. https://doi.org/10.1016/j. ympev.2004.11.014
  24. Matheny, P.B., Aime, M.C., Bougher, N.L., Buyck, B., Desjardin, D.E., Horak, E. & Hibbett, D.S. (2009) Out of the Palaeotropics? Historical biogeography and diversification of the cosmopolitan ectomycorrhizal mushroom family Inocybaceae. Journal of Biogeography 36 (4): 577–592. https://doi.org/10.1111/j.1365-2699.2008.02055.x
  25. Matheny, P.B., Hobbs, A.M. & Fernando, E.R. (2019) Genera of Inocybaceae: New skin for the old ceremony. Mycologia 112: 1–38. https://doi.org/10.1080/00275514.2019.1668906
  26. Muñoz, J.A. (2022) Inosperma adaequatum (Britzelm.) Matheny & Esteve-Rav. [online]. Micológica-Barakaldo, Fungal Data Sheets, no. 649 (Updated on May 25, 2022). Available from: https://micologica-barakaldo.org/Inosperma-adaequatum/ (accessed 16 October 2024)
  27. Munsell (1975) Munsell Soil Color Charts. Baltimore, MD.
  28. Naseer, A., Khalid, A.N. & Smith, M.E. (2017) Inocybe shawarensis sp. nov. in the Inosperma clade from Pakistan. Mycotaxon 132 (4): 909–918. https://doi.org/10.5248/132.909
  29. O’Reilly, P. (2016) Fascinated by Fungi. 2nd ed. Coch-y-bonddu Books.
  30. Parnmen, S., Nooron, N., Leudang, S., Sikaphan, S., Polputpisatkul, D., Pringsulaka, O., Binchai, S. & Rangsiruji, A. (2021) Foodborne illness caused by muscarine-containing mushrooms and identification of mushroom remnants using phylogenetics and LC-MS/MS. Food Control 128: 108182. https://doi.org/10.1016/j.foodcont.2021.108182
  31. Pradeep, C.K., Vrinda, K.B., Varghese, S.P., Korotkin, H.B. & Matheny, P.B. (2016) New and noteworthy species of Inocybe (Agaricales) from tropical India. Mycological Progress 15: 24. https://doi.org/10.1007/s11557-016-1174-z.
  32. Rambaut, A. (2012) FigTree v1.4.2. Available from: http://tree.bio.ed.ac.uk/software/figtree (accessed 25 February 2025)
  33. Rathnayaka, A.R., Tennakoon, D.S., Jones, G.E., Wanasinghe, D.N., Bhat, D.J., Priyashantha, A.H., Stephenson, S.L., Tibpromma, S. & Karunarathna, S.C. (2024) Significance of precise documentation of hosts and geospatial data of fungal collections, with an emphasis on plant-associated fungi. New Zealand Journal of Botany. https://doi.org/10.1080/0028825X.2024.2381734
  34. Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematics Biology 61: 539–542. https://doi.org/10.1093/sysbio/sys029
  35. Ryberg, M. & Matheny, P.B. (2012) Asynchronous origins of ectomycorrhizal clades of Agaricales. Proceedings of the Royal Society B: Biological Sciences 279 (1735): 2003–2011. https://doi.org/10.1098/rspb.2011.2428
  36. Ryberg, M., Nilsson, R.H., Kristiansson, E., To pel, M., Jacobsson, S. & Larsson, E. (2008) Mining metadata from unidentified ITS sequences in GenBank: A case study in Inocybe (Basidiomycota). BMC Evolutionary Biology 8: 50. https://doi.org/10.1186/1471-2148-8-50
  37. Schoch, C.L., Robbertse, B., Robert, V., Vu, D., Cardinali, G., Irinyi, L., Meyer, W., Nilsson, R.H., Hughes, K., Miller, A.N. & Kirk, P.M. (2014) Finding needles in haystacks: linking scientific names, reference specimens and molecular data for Fungi. Database 2014: p.bau061.
  38. Sultana, K., Rauf, C.A., Riaz, A., Naz, F., Irshad, G. & Haque, M.I. (2011) Checklist of Agarics of Kaghan valley-1. Pakistan Journal of Botany 43 (3): 1777–1787.
  39. Trifinopoulos, J., Nguyen, L.T., von Haeseler, A. & Minh, B.Q. (2016) W-IQ-TREE: a fast online phylogenetic tool for maximum likelihood analyses. Nucleic Acids Research 44 (W1): W232–W235. https://doi.org/10.1093/nar/gkw256
  40. Vellinga, E.C. (2001) Leucoagaricus. Flora Agaricina Neerlandica 5: 85–108.
  41. Werle, E., Schneider, C., Renner, M., Völker, M. & Fiehn, W. (1994) Convenient single-step, one tube purification of PCR products for direct sequencing. Nucleic Acids Research 22: 4354–4355. https://doi.org/10.1093/nar/22.20.435
  42. White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelf, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR protocols: A guide to methods applications. Academic Press, New York, pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
  43. Wurzbacher, C., Larsson, E., Bengtsson, Palme, J., Van den Wyngaert, S., Svantesson, S., Kristiansson, E. & Nilsson, R.H. (2019) Introducing ribosomal tandem repeat barcoding for fungi. Molecular Ecology Resources 19 (1): 118–127. https://doi.org/10.1111/1755-0998.12944

How to Cite

Sana, Kiran, M. & Ali, W. (2025) Inosperma pakistanicum sp. nov. (Inocybaceae, Agaricales) from Swat, Pakistan. Phytotaxa 690 (1): 77–88. https://doi.org/10.11646/phytotaxa.690.1.6