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Issue: Vol. 684 No. 1: 5 Feb. 2025
Type: Article
Published: 2025-02-05
DOI: 10.11646/phytotaxa.684.1.4
Page range: 78-92
Abstract views: 229
PDF downloaded: 22

Exobasidium setsutaiense sp. nov. (Exobasidiaceae) causing leaf blister on Rhododendron semibarbatum in Japan

Visiting researcher, Kanagawa Prefectural Museum of Natural History, 499 Iriuda, Odawara, Kanagawa 250-0013, Japan. Visiting researcher, Research and Education Center for Natural Sciences, Keio University, 4-1-1 Hiyoshi, Kohoku, Yokohama, Kanagawa Pref., 223-8521 Japan.
Kanagawa Prefectural Museum of Natural History, 499 Iriuda, Odawara, Kanagawa 250-0013, Japan
Department of Biology, Faculty of Economics, Keio University, 4-1-1 Hiyoshi, Kohoku, Yokohama, Kanagawa Pref., 223-8521 Japan
basidium basidiospore Exobasidium butleri Iwate Rhododendron arboreum Tochigi Basidiomycota Fungi

Abstract

Exobasidium setsutaiense sp. nov. is proposed to accommodate the causal agent of leaf blister on Rhododendron semibarbatum. After the examination of the herbarium materials (IUM) and newly collected fresh materials, the symptom on R. semibarbatum was clearly different from those on Rhododendron arboreum, the host of Exobasidium butleri. Morphology of basidiospores was slightly curved towards a hilum with 3–4 septa, whereas the number of septa in basidiospores of E. butleri was 0–1. The mode of germination of basidiospores was by a germ tube or by budding. The leaf blister was characterized by chlorotic leaf spot where the lesion was slightly thickened by the hyperplasia of spongy mesophyll cells. Molecular analyses of the concatenated ITS + LSU sequences supported the independence of E. setsutaiense.

References

  1. Begerow, D., Bauer, R. & Oberwinkler, F. (2002) The Exobasidiales: an evolutionary hypothesis. Mycological Progress 1: 187–199. https://doi.org/10.1007/s11557-006-0018-7
  2. Don, G. (1834) A general history of the dichlamydeous plants. Vol. 3. London, J.G. and F. Rivington, pp. 843. https://doi.org/10.5962/bhl.title.502
  3. Ezuka, A. (1990) Notes on some species of Exobasidium in Japan (I). Transactions of Mycological Society of Japan 31: 375–388. [In Japanese]
  4. Ezuka, A. (1991) Notes on some species of Exobasidium in Japan (III). Transactions of Mycological Society of Japan 32: 71–86. [In Japanese]
  5. Felsenstein, J. (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 6: 227–242. https://doi.org/10.2307/2408678
  6. Gardes, M. & Bruns, T.D. (1993) ITS primers with enhanced specificity for basidiomycetes—application to the identification of mycorrhizae and rusts. Molecular ecology 2 (2) 113–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
  7. Hennings, P. (1902) Fungi japonica IV. Botanische Jahrbücher fur Systematik, Pflanzengeschichte und Pflanzengeographie 31: 728–742.
  8. Hennings, P. (1903) Fungi japonica III. Botanische Jahrbücher fur Systematik, Pflanzengeschichte und Pflanzengeographie 32: 34–46.
  9. Ito, S. (1955) Exobasidium Butleri SYD., No.4 Auriculariales, Tremellales, Dacrymycetales, Aphyllophorales (Polyporales), Vol. II Basidiomycetes. In: Mycological Flora of Japan. Yokendo. Tokyo, pp. 55. [In Japanese]
  10. Ito, S. & Otani, Y. (1958) Two new species of Exobasidium. Transactions of Mycological Society of Japan 1: 3–4. [In Japanese]
  11. Izumitsu, K., Hatoh, K., Sumita, T., Kitade, Y., Morita, A., Gafur, A., Ohta, A., Kawai, M., Yamanaka, T., Neda, H., Ota, Y. & Tanaka, C. (2012) Rapid and simple preparation of mushroom DNA directly from colonies and fruiting bodies for PCR. Mycoscience 53: 396–401. https://doi.org/10.1007/S10267-012-0182-3
  12. Kirk, P.M., Cannon, P.F., David, J.C. & Stalpers, J.A. (Eds.) (2001) Dictionary of the Fungi. 9th Edition. CABI Publishing, Wallingford. pp. 193, 332–333.
  13. Kurashige, Y., Mine, M., Kobayashi, N., Handa, T., Takayanagi, K. & Yukawa, T. (1998) Investigation of Sectional Relationships in the Genus Rhododendron (Ericaceae) Based on matK Sequences. Journal of Japanese Botany 73: 143–154.
  14. Kurashige, Y., Etoh, J.-I., Handa, T., Takayanagi, K. & Yukawa, T. (2001) Sectional relationships in the genus Rhododendron (Ericaceae): evidence from matK and trnK intron sequences. Plant Systematics and Evolution 228: l–14. https://doi.org/10.1007/s006060170033
  15. Kurashige, Y. (2017) Genus Rhododendron, In: Ohashi, H., Kadota, Y., Murata, J. & Yonekura, K. (Eds.) Wildflowers of Japan, revised edition. Vol. 4 MALVACEAE~APOCYNACEAE. Heibonsya Limited Publishers, Tokyo, pp. 250. [ISBN978-4-582-53534-1]
  16. Linnaeus, C. (1753) Rhododendron L. In: Species Plantarum, Edition 1. pp. 392.
  17. Madeira, F., Pearce, M., Tivey, A.R.N., Basutkar, P., Lee, J., Edbali, O., Madhusoodanan, N., Kolesnikov, A. & Lopez, R. (2022) Search and sequence analysis tools services from EMBL-EBI in 2022. Nucleic Acids Research 50 (W1): W276–W279. https://doi.org/10.1093/nar/gkac240
  18. Maximowicz, C.J. (1871) Courtes diagnoses de quelques nouvelles plantes du Japon et de la Mandchourie. Septième decade. Bulletin de l'Académie impériale des sciences de St.-Pétersbourg ser. 3 v. 15: 229–230.
  19. Nagao, H., Ezuka, A., Ohkubo, H. & Kakishima, M. (2001) A new species of Exobasidium causing witches' broom on Rhododendron wadanum. Mycoscience 42: 549–554. https://doi.org/10.1007/BF02460953
  20. Nagao, H., Akimoto, M., Kishi, K., Ezuka, A. & Kakishima, M. (2003a) Exobasidium dubium and E. miyabei sp. nov. causing Exobasidium leaf blisters on Rhododendron spp. in Japan. Mycoscience 44: 1–9. https://doi.org/10.1007/S10267-002-0068-X
  21. Nagao, H., Ogawa, S., Sato, T. & Kakishima, M. (2003b) Exobasidium symploci-japonicae var. caprogenum var. nov. causing Exobasidium fruit deformation on Symplocos lucida in Japan. Mycoscience 44: 369–375. https://doi.org/10.1007/S10267-003-0126-Z
  22. Nagao, H. & Kasuya, T. (2023) The host-pathogen association of Exobasidium in Japan inferred from molecular phylogeny of ITS and large subunit rDNA sequences. The Hiyoshi review of natural science 70: 1–21
  23. Nannfeldt, J.A. (1981) Exobasidium a taxonomic reassessment applied to the European species. Symbolae Botanicae Upsalienses 23: 1–72.
  24. O’Donnell, K.L. (1993) Fusarium and its near relatives. In: Reynolds, D.R. & Taylor, J.W. (eds.) The fungal holomorph: mitotic, meiotic, and pleomorphic speciation in fungal systematics. CAB international, Wallingford, pp. 225–233.
  25. Orihara, T., Smith, M.E., Shimomura, N., Iwase, K. & Maekawa, N. (2012) Diversity and systematics of the sequestrate genus Octaviania in Japan: two new subgenera and eleven new species. Persoonia 28: 85–112. https://doi.org/10.3767/003158512X650121
  26. Raciborski, M. (1909) Mycotheca polonica. Kosmos 34: 1166–1172.
  27. Rayner, R.W. (1970) A Mycological colour chart. Commonwealth Mycological Institute, Kew, Surrey & British Mycological Society.
  28. Reaz, R., Bayzid, M.S. & Rahman, M.S. (2014) Accurate Phylogenetic Tree Reconstruction from Quartets: A Heuristic Approach. PLoS ONE 9 (8): e104008. https://doi.org/10.1371/journal.pone.0104008
  29. Sawada, K. (1950) Fungi from the North-Eastern Region of Honshu, Japan (2) Basidiomycetes. Tohoku Biological 1: 3–4.
  30. Schmidt, H.A. & von Haeseler, A. (2003) Maximum-Likelihood Analysis Using Tree-Puzzle. Current Protocols in Bioinformatics 1: 6.6.1–6.6.23. [John Wiley & Sons, Inc.] https://doi.org/10.1002/0471250953.bi0606s01
  31. Singh, S.C. & Nisha (1974) Exobasidium butleri in Nepal. Indian Phytopathology 27: 387–389.
  32. Smith, J.E. (1805) Rhododendron arboreum. In: Exotic Botany 1 (9). R. Taylor and Co. London. pp. 1–118.
  33. Stephen, F.A., Thomas, L.M., Alejandro, A.S., Jinghui, Z., Zheng, Z., Webb, M. & David, J.L. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research 25: 3389–3402. https://doi.org/10.1093/nar/25.17.3389
  34. Swofford, D.L. (1998) PAUP*4.0. Phylogenetic analysis using parsimony. Sinauer Associates, Sunderland, Massachusetts.
  35. Sydow, H., Sydow, P. & Butler, E.J. (1912) Fungi Indiae Orientalis IV. Annales Mycologici 10: 243–280.
  36. White, T.J., Bruns, T., Lee, S.B. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Gelfand, M., Sninsky, D. & White, T. (eds.) PCR protocols: a guide to methods and applications. Academic Press, San Diego. pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
  37. Woronin, M. (1867) Exobasidium Vaccinii. Berichte über die Verhandlungen der Naturforschenden Gesellschaft zu Freiburg i. Br.. Freiburg im Breisgau 4: 397–416.

How to Cite

Nagao, H., Orihara, T. & Kasuya, T. (2025) Exobasidium setsutaiense sp. nov. (Exobasidiaceae) causing leaf blister on Rhododendron semibarbatum in Japan. Phytotaxa 684 (1): 78–92. https://doi.org/10.11646/phytotaxa.684.1.4