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Issue: Vol. 665 No. 3: 24 Sept. 2024
Type: Article
Published: 2024-09-24
DOI: 10.11646/phytotaxa.665.3.6
Page range: 243-252
Abstract views: 168
PDF downloaded: 11

A new endophytic species, Diaporthe litseae, from medicinal plants in Guizhou Province, China

Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand. School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand. Department of Plant Pathology, College of Agriculture, Guizhou University, Guiyang 550025, China.
School of Food and Pharmaceutical Engineering, Guizhou Institute of Technology, Guiyang 550025, Guizhou, China
Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 22452, 11495 Riyadh, Saudi Arabia
Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand. School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand. Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 22452, 11495 Riyadh, Saudi Arabia.
Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand; School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand
Diaporthaceae endophyte Litsea phylogeny taxonomy Fungi

Abstract

During our study on microfungi associated with medicinal plants in China, a new endophytic taxon, Diaporthe litseae was isolated from healthy leaves of Litsea kobuskiana collected in Guizhou Province, China. The new species is characterized by its coelomycetous asexual morph, which has dark brown to black, globose conidiomata, cylindrical, aseptate conidiophores, phialidic conidiogenous cells, and fusiform, hyaline, aseptate, guttulate conidia. The phylogenetic analyses of combined ITS, tef1-α, β-tubulin, cal and his3 sequence data indicate D. litseae is phylogenetically distinct from other species. A detailed description and illustration for D. litseae are provided herein.

References

  1. Abeywickrama, P.D., Camporesi, E., Jayawardena, R.S., Hyde, K.D., Yan, J.Y., Zhang, W. & Li, X.H. (2022) Novel and surprising host associations of Diaporthe (Diaporthaceae, Diaporthales) species from Italy. Chiang Mai Journal of Science 49: 223–247. https://doi.org/10.12982/CMJS.2022.028
  2. Al-Ani, L.K.T. (2019) The importance of endophytic fungi from the medicinal plant: diversity, natural bioactive compounds, and control of plant pathogens. In: Egamberdieva, D. & Tiezzi, A. (Eds.) Medically Important Plant Biomes: Source of Secondary Metabolites. Springer Singapore, Singapore, pp. 189–238. https://doi.org/10.1007/978-981-13-9566-6_9
  3. Capella-Gutierrez, S., Silla-Martinez, J.M. & Gabaldon, T. (2009) trimAl: A tool for automated alignment trimming in large-scale phylogenetic analyses. Bioinformatics 25: 1972–1973. https://doi.org/10.1093/bioinformatics/btp348
  4. Chaisiri, C.C., Liu, X.Y., Lin, Y. & Luo, C.X. (2022) Diaporthe citri: A fungal pathogen causing melanose disease. Plants 11: 1600. https://doi.org/10.3390/plants11121600
  5. Chen, Y.C., Wang, Y.D., Han, X.J., Si, L.L., Wu, Q.K. & Lin, L.Y. (2013) Biology and chemistry of Litsea cubeba, a promising industrial tree in China. Journal of Essential Oil Research 25: 103–111. https://doi.org/10.1080/10412905.2012.751559
  6. Chethana, K.W.T., Manawasinghe, I.S., Hurdeal, V.G., Bhunjun, C.S., Appadoo, M.A., Gentekaki, E., Raspé, O., Promputtha, I. & Hyde, K.D. (2021) What are fungal species and how to delineate them? Fungal Diversity 109: 1–25. https://doi.org/10.1007/s13225-021-00483-9
  7. Dissanayake, A.J., Chen, Y.Y. & Liu, J.K. (2020) Unravelling Diaporthe species associated with woody hosts from karst formations (Guizhou) in China. Journal of Fungi 6: 251. https://doi.org/10.3390/jof6040251
  8. Dissanayake, A.J., Phillips, A.J.L., Hyde, K.D., Yan, J.Y. & Li, X.H. (2017) The current status of species in Diaporthe. Mycosphere 8: 1106–1156. https://doi.org/10.5943/mycosphere/8/5/5
  9. Dissanayake, A.J., Zhu, J.T., Chen, Y.Y., Maharachchikumbura, S.S.N., Hyde, K.D. & Liu, J.K. (2024) A re-evaluation of Diaporthe: refining the boundaries of species and species complexes. Fungal Diversity 126: 1–125. https://doi.org/10.1007/s13225-024-00538-7
  10. Dong, Z.Y., Manawasinghe, I.S., Huang, Y.H., Shu, Y.X., Phillips, A.J.L., Dissanayake, A.J., Hyde, K.D., Xiang, M.M. & Luo, M. (2021) Endophytic Diaporthe associated with Citrus grandis cv. Tomentosa in China. Frontiers in Microbiology 11: 609387. https://doi.org/10.3389/fmicb.2020.609387
  11. Gao, Y., Liu, F., Duan, W., Crous, P.W. & Cai, L. (2017) Diaporthe is paraphyletic. IMA Fungus 8: 153–187. https://doi.org/10.1007/BF03449470
  12. Gomes, R.R., Glienke, C., Videira, S.I.R., Lombard, L., Groenewald, J.Z. & Crous, P.W. (2013) Diaporthe: a genus of endophytic, saprobic and plant pathogenic fungi. Persoonia 31: 1–41. https://doi.org/10.3767/003158513X666844
  13. Hongsanan, S., Norphanphoun, C., Senanayake, I.C., Jayawardena, R.S., Manawasinghe, I.S., Abeywickrama, P.D., Khuna, S., Suwannarach, N., Senwanna, C., Monkai, J., Hyde, K.D., Gentekaki, E. & Bhunjun, C.S. (2023) Annotated notes on Diaporthe species. Mycosphere 14: 918–1189. https://doi.org/10.5943/mycosphere/14/1/12
  14. Huang, F., Hou, X., Dewdney, M.M., Fu, Y.S., Chen, G.Q., Hyde, K.D. & Li, H.Y. (2013) Diaporthe species occurring on citrus in China. Fungal Diversity 61: 237–250. https://doi.org/10.1007/s13225-013-0245-6
  15. Huang, F., Udayanga, D., Wang, X.H., Hou, X., Mei, X.F., Fu, Y.S., Hyde, K.D. & Li, H.Y. (2015) Endophytic Diaporthe associated with citrus: A phylogenetic reassessment with seven new species from China. Fungal Biology 119: 331–347. https://doi.org/10.1016/j.funbio.2015.02.006
  16. Huang, S.T., Xia, J.W., Zhang, X.G. & Sun, W.X. (2021a) Morphological and phylogenetic analyses reveal three new species of Diaporthe from Yunnan, China. MycoKeys 78: 49–77. https://doi.org/10.3897/mycokeys.78.60878
  17. Huang, X.S., Zhou, D.X., Liang, Y., Liu, X.B., Cao, F., Qin, Y.Y., Mo, T.X., Xu, Z.L., Li, J. & Yang, R.Y. (2021b) Cytochalasins from endophytic Diaporthe sp. GDG-118. Natural Product Research 35: 3396–3403. https://doi.org/10.1080/14786419.2019.1700504
  18. Hyde, K.D., Norphanphoun, C., Abreu, V.P., Bazzicalupo, A., Thilini Chethana, K.W., Clericuzio, M., Dayarathne, M.C., Dissanayake, A.J., Ekanayaka, A.H., He, M.Q., Hongsanan, S., Huang, S.K., Jayasiri, S.C., Jayawardena, R.S., Karunarathna, A., Konta, S., Kušan, I., Lee, H., Li, J.F., Lin, C.G., Liu, N.G., Lu, Y.Z., Luo, Z.L., Manawasinghe, I.S., Mapook, A., Perera, R.H., Phookamsak, R., Phukhamsakda, C., Siedlecki, I., Soares, A.M., Tennakoon, D.S., Tian, Q., Tibpromma, S., Wanasinghe, D.N., Xiao, Y.P., Yang, J., Zeng, X.Y., Abdel-Aziz, F.A., Li, W.J., Senanayake, I.C., Shang, Q.J., Daranagama, D.A., de Silva, N.I., Thambugala, K.M., Abdel-Wahab, M.A., Bahkali, A.H., Berbee, M.L., Boonmee, S., Bhat, D.J., Bulgakov, T.S., Buyck, B., Camporesi, E., Castañeda-Ruiz, R.F., Chomnunti, P., Doilom, M., Dovana, F., Gibertoni, T.B., Jadan, M., Jeewon, R., Jones, E.B.G., Kang, J.C., Karunarathna, S.C., Lim, Y.W., Liu, J.K., Liu, Z.Y., Plautz, H.L., Lumyong, S., Maharachchikumbura, S.S.N., Matočec, N., McKenzie, E.H.C., Mešić, A., Miller, D., Pawłowska, J., Pereira, O.L., Promputtha, I., Romero, A.I., Ryvarden, L., Su, H.Y., Suetrong, S., Tkalčec, Z., Vizzini, A., Wen, T.C., Wisitrassameewong, K., Wrzosek, M., Xu, J.C., Zhao, Q., Zhao, R.L. & Mortimer, P.E. (2017) Fungal diversity notes 603–708: taxonomic and phylogenetic notes on genera and species. Fungal Diversity 87: 1–235. https://doi.org/10.1007/s13225-017-0383-3
  19. Hyde, K.D., Tennakoon, D.S., Jeewon, R., Bhat, D.J., Maharachchikumbura, S.S.N., Rossi, W., Leonardi, M., Lee, H.B., Mun, H.Y., Houbraken, J., Nguyen, T.T.T., Jeon, S.J., Frisvad, J.C., Wanasinghe, D.N., Lücking, R., Aptroot, A., Cáceres, M.E.S., Karunarathna, S.C., Hongsanan, S., Phookamsak, R., de Silva, N.I., Thambugala, K.M., Jayawardena, R.S., Senanayake, I.C., Boonmee, S., Chen, J., Luo, Z.L., Phukhamsakda, C., Pereira, O.L., Abreu, V.P., Rosado, A.W.C., Bart, B., Randrianjohany, E., Hofstetter, V., Gibertoni, T.B., Soares, A.M.d.S., Plautz, H.L., Sotão, H.M.P., Xavier, W.K.S., Bezerra, J.D.P., de Oliveira, T.G.L., de Souza-Motta, C.M., Magalhães, O.M.C., Bundhun, D., Harishchandra, D., Manawasinghe, I.S., Dong, W., Zhang, S.N., Bao, D.F., Samarakoon, M.C., Pem, D., Karunarathna, A., Lin, C.G., Yang, J., Perera, R.H., Kumar, V., Huang, S.K., Dayarathne, M.C., Ekanayaka, A.H., Jayasiri, S.C., Xiao, Y.P., Konta, S., Niskanen, T., Liimatainen, K., Dai, Y.C., Ji, X.H., Tian, X.M., Mešić, A., Singh, S.K., Phutthacharoen, K., Cai, L., Sorvongxay, T., Thiyagaraja, V., Norphanphoun, C., Chaiwan, N., Lu, Y.Z., Jiang, H.B., Zhang, J.F., Abeywickrama, P.D., Aluthmuhandiram, J.V.S., Brahmanage, R.S., Zeng, M., Chethana, T., Wei, D.P., Réblová, M., Fournier, J., Nekvindová, J., do Nascimento Barbosa, R., dos Santos, J.E.F., de Oliveira, N.T., Li, G.J., Ertz, D., Shang, Q.J., Phillips, A.J.L., Kuo, C.H., Camporesi, E., Bulgakov, T.S., Lumyong, S., Jones, E.B.G., Chomnunti, P., Gentekaki, E., Bungartz, F., Zeng, X.Y., Fryar, S., Tkalčec, Z., Liang, J.M., Li, G.S., Wen, T.C., Singh, P.N., Gafforov, Y., Promputtha, I., Yasanthika, E., Goonasekara, I.D., Zhao, R.L., Zhao, Q., Kirk, P.M., Liu, J.K., Yan, J.Y., Mortimer, P.E., Xu, J.C. & Doilom, M. (2019) Fungal diversity notes 1036–1150: taxonomic and phylogenetic contributions on genera and species of fungal taxa. Fungal Diversity 96: 1–242. https://doi.org/10.1007/s13225-019-00430-9
  20. Jayasiri, S.C., Hyde, K.D., Ariyawansa, H.A., Bhat, J., Buyck, B., Cai, L., Dai, Y.C., Abd-Elsalam, K.A., Ertz, D., Hidayat, I., Jeewon, R., Jones, E.B.G., Bahkali, A.H., Karunarathna, S.C., Liu, J.K., Luangsa-ard, J.J., Lumbsch, H.T., Maharachchikumbura, S.S.N., McKenzie, E.H.C., Moncalvo, J.M., Ghobad-Nejhad, M., Nilsson, H., Pang, K.L., Pereira, O.L., Phillips, A.J.L., Raspé, O., Rollins, A.W., Romero, A.I., Etayo, J., Selçuk, F., Stephenson, S.L., Suetrong, S., Taylor, J.E., Tsui, C.K.M., Vizzini, A., Abdel-Wahab, M.A., Wen, T.C., Boonmee, S., Dai, D.Q., Daranagama, D.A., Dissanayake, A.J., Ekanayaka, A.H., Fryar, S.C., Hongsanan, S., Jayawardena, R.S., Li, W.J., Perera, R.H., Phookamsak, R., de Silva, N.I., Thambugala, K.M., Tian, Q., Wijayawardene, N.N., Zhao, R.L., Zhao, Q., Kang, J.C. & Promputtha, I. (2015) The Faces of Fungi database: fungal names linked with morphology, phylogeny and human impacts. Fungal Diversity 74: 3–18. https://doi.org/10.1007/s13225-015-0351-8
  21. Jayawardena, R.S., Hyde, K.D., de Farias, A.R.G., Bhunjun, C.S., Ferdinandez, H.S., Manamgoda, D.S., Udayanga, D., Herath, I.S., Thambugala, K.M., Manawasinghe, I.S., Gajanayake, A.J., Samarakoon, B.C., Bundhun, D., Gomdola, D., Huanraluek, N., Sun, Y.-R., Tang, X., Promputtha, I. & Thines, M. (2021) What is a species in fungal plant pathogens? Fungal Diversity 109: 239–266. https://doi.org/10.1007/s13225-021-00484-8
  22. Jeewon, R. & Hyde, K.D. (2016) Establishing species boundaries and new taxa among fungi: recommendations to resolve taxonomic ambiguities. Mycosphere 7: 1669–1677. https://doi.org/10.5943/mycosphere/7/11/4
  23. Kamle, M., Mahato, D.K., Lee, K.E., Bajpai, V.K., Gajurel, P.R., Gu, K.S. & Kumar, P. (2019) Ethnopharmacological properties and medicinal uses of Litsea cubeba. Plants 8: 150. https://doi.org/10.3390/plants8060150
  24. Khamis, S. & Nizam, M.S. (2013) Distribution of Litsea complex (Lauraceae) and their association with soil properties in different forest types of Peninsular Malaysia. AIP Conference Proceedings 1571: 363–367. https://doi.org/10.1063/1.4858684
  25. Kong, D.G., Zhao, Y., Li, G.H., Chen, B.J., Wang, X.N., Zhou, H.L., Lou, H.X., Ren, D.M. & Shen, T. (2015) The genus Litsea in traditional Chinese medicine: An ethnomedical, phytochemical and pharmacological review. Journal of Ethnopharmacology 164: 256–264. https://doi.org/10.1016/j.jep.2015.02.020
  26. Larsson, A. (2014) AliView: A fast and lightweight alignment viewer and editor for large datasets. Bioinformatics 30: 3276–3278. https://doi.org/10.1093/bioinformatics/btu531
  27. Li, G., Kusari, S., Kusari, P., Kayser, O. & Spiteller, M. (2015) Endophytic Diaporthe sp. LG23 produces a potent antibacterial tetracyclic triterpenoid. Journal of Natural Products 78: 2128–2132. https://doi.org/10.1021/acs.jnatprod.5b00170
  28. Liu, T.T. & Yang, T.S. (2012) Antimicrobial impact of the components of essential oil of Litsea cubeba from Taiwan and antimicrobial activity of the oil in food systems. International Journal of Food Microbiology 156: 68–75. https://doi.org/10.1016/j.ijfoodmicro.2012.03.005
  29. Lu, Q.T., Zhang, J.Y., Sun, Y.R., Tang, X., Lu, Y.Z. & Zhang, Z. (2022) Diaporthe orixae sp. nov., an endophytic species isolated from Orixa japonica in southern China. Phytotaxa 544: 37–51. https://doi.org/10.11646/phytotaxa.544.1.3
  30. Luo, M., Guo, W., Zhao, M.P., Manawasinghe, I.S., Guarnaccia, V., Liu, J.W., Hyde, K.D., Dong, Z.Y. & You, C.P. (2022) Endophytic Diaporthe associated with Morinda officinalis in China. Journal of Fungi 8: 806. https://doi.org/10.3390/jof8080806
  31. Madasi, A., Ajmeera, A., Renuka, G., Vemireddy, B. & Vanteru, K.R. (2021) Production of extracellular enzymes, anti-microbial and other agriculturally important metabolites by fungal endophytes of Litsea glutinosa (Lour.) CB Rob, a medicinal plant. Journal of Pure and Applied Microbiology 15: 2317–2329. https://doi.org/10.22207/JPAM.15.4.56
  32. Maharachchikumbura, S.S.N., Chen, Y., Ariyawansa, H.A., Hyde, K.D., Haelewaters, D., Perera, R.H., Samarakoon, M.C., Wanasinghe, D.N., Bustamante, D.E., Liu, J.K., Lawrence, D.P., Cheewangkoon, R. & Stadler, M. (2021) Integrative approaches for species delimitation in Ascomycota. Fungal Diversity 109: 155–179. https://doi.org/10.1007/s13225-021-00486-6
  33. Norphanphoun, C., Gentekaki, E., Hongsanan, S., Jayawardena, R., Senanayake, I., Manawasinghe, I.S., Abeywickrama, P.D., Bhunjun, C.S. & Hyde, K.D. (2022) Diaporthe: Formalizing the species-group concept. Mycosphere 13: 752–819. https://doi.org/10.5943/mycosphere/13/1/9
  34. Nylander, J.A.A. (2008) MrModeltest 2.3. Uppsala, Department of Systematic Zoology, Uppsala University.
  35. Perera, R.H., Hyde, K.D., Peršoh, D., Jones, E.B.G., Liu, J.K. & Liu, Z.Y. (2018) Additions to wild seed and fruit fungi 1: The sexual morph of Diaporthe rosae on Magnolia champaca and Senna siamea fruits in Thailand. Mycosphere 9: 256–270. https://doi.org/10.5943/mycosphere/9/2/7
  36. Ronquist, F., Teslenko, M., Van Der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61: 539–542. https://doi.org/10.1093/sysbio/sys029
  37. Saravanakumar, K., Sriram, B., Sathiyaseelan, A., Hu, X., Mariadoss, A.V.A., MubarakAli, D. & Wang, M.H. (2021) Molecular identification, volatile metabolites profiling, and bioactivities of an indigenous endophytic fungus (Diaporthe sp.). Process Biochemistry 102: 72–81. https://doi.org/10.1016/j.procbio.2020.12.002
  38. Senanayake, I., Rathnayaka, A.R., Marasinghe, D.S., Calabon, M.S., Gentekaki, E., Lee, H.B., Hurdeal, V.G., Pem, D., Dissanayake, L.S., Wijesinghe, S.N., Bundhun, D., Nguyen, T.T., Goonasekara, I.D., Abeywickrama, P.D., Bhunjun, C.S., Jayawardena, R.S., Wanasinghe, D.N., Jeewon, R., Bhat, D.J. & Xiang, M.M. (2020) Morphological approaches in studying fungi: collection, examination, isolation, sporulation and preservation. Mycosphere 11: 2678–2754. https://doi.org/10.5943/mycosphere/11/1/20
  39. Silva Santos, S.d., Silva, A.A.d., Polonio, J.C., Polli, A.D., Orlandelli, R.C., Oliveira, J.A.d.S., Brandão-Filho, J.U.T. & Pamphile, J.A. (2022) Influence of plant growth-promoting endophytes Colletotrichum siamense and Diaporthe masirevici on tomato plants (Lycopersicon esculentum Mill.). Mycology 13: 257–270. https://doi.org/10.1080/21501203.2022.2050825
  40. Song, X.Y., Wang, H.H., Ren, F., Wang, K., Dou, G., Lv, X., Yan, D.H. & Strobel, G. (2019) An endophytic Diaporthe apiculatum produces monoterpenes with inhibitory activity against phytopathogenic fungi. Antibiotics 8: 231. https://doi.org/10.3390/antibiotics8040231
  41. Tan, C., Ferguson, D.K. & Yang, Y. (2024) Incorrect and incomplete distribution data can mislead species modeling: a case study of the endangered Litsea auriculata (Lauraceae). [preprint] https://doi.org/10.21203/rs.3.rs-3978669/v1
  42. Thomidis, T. & Michailides, T.J. (2009) Studies on Diaporthe eres as a new pathogen of peach trees in Greece. Plant Disease 93: 1293–1297. https://doi.org/10.1094/PDIS-93-12-1293
  43. Trifinopoulos, J., Nguyen, L.T., von Haeseler, A. & Minh, B.Q. (2016) W-IQ-TREE: a fast online phylogenetic tool for maximum likelihood analysis. Nucleic Acids Research 44: W232–W235. https://doi.org/10.1093/nar/gkw256
  44. Tsitlakidou, P., Tasopoulos, N., Chatzopoulou, P. & Mourtzinos, I. (2023) Current status, technology, regulation and future perspectives of essential oils usage in the food and drink industry. Journal of the Science of Food and Agriculture 103: 6727–6751. https://doi.org/10.1002/jsfa.12695
  45. Udayanga, D., Castlebury, L.A., Rossman, A.Y. & Hyde, K.D. (2014a) Insights into the genus Diaporthe: phylogenetic species delimitation in the D. eres species complex. Fungal Diversity 67: 203–229. https://doi.org/10.1007/s13225-014-0297-2
  46. Udayanga, D., Castlebury, L.A., Rossman, A.Y. & Hyde, K.D. (2014b) Species limits in Diaporthe: molecular re-assessment of D. citri, D. cytosporella, D. foeniculina and D. rudis. Persoonia 32: 83–101. https://doi.org/10.3767/003158514X679984
  47. Vaidya, G., Lohman, D.J. & Meier, R. (2011) SequenceMatrix: concatenation software for the fast assembly of multi-gene datasets with character set and codon information. Cladistics 27: 171–180. https://doi.org/10.1111/j.1096-0031.2010.00329.x
  48. Wang, J.Y., Xu, X.H., Mao, L.J., Lao, J.P., Lin, F.C., Yuan, Z.L. & Zhang, C.L. (2014) Endophytic Diaporthe from Southeast China are genetically diverse based on multi-locus phylogeny analyses. World Journal of Microbiology and Biotechnology 30: 237–243. https://doi.org/10.1007/s11274-013-1446-6
  49. Wang, S.Y., McKenzie, E.H.C., Phillips, A.J.L., Li, Y. & Wang, Y. (2022) Taxonomy and multigene phylogeny of Diaporthales in Guizhou Province, China. Journal of Fungi 8: 1301. https://doi.org/10.3390/jof8121301
  50. Wang, Y.S., Wen, Z.Q., Li, B.T., Zhang, H.B. & Yang, J.H. (2016) Ethnobotany, phytochemistry, and pharmacology of the genus Litsea: An update. Journal of Ethnopharmacology 181: 66–107. https://doi.org/10.1016/j.jep.2016.01.032
  51. Wu, F., Yang, D.C., Zhang, L.P., Chen, Y.L., Hu, X.K., Li, L. & Liang, J.S. (2019) Diversity estimation and antimicrobial activity of culturable endophytic fungi from Litsea cubeba (Lour.) Pers. in China. Forests 10: 33. https://doi.org/10.3390/f10010033
  52. Xiao, Y.S., Zou, M.Y., Zhou, X., Yang, C.X. & Cui, C.Y. (2023) First report of postharvest fruit brown rot disease on navel orange caused by Diaporthe sojae in China. Plant Disease 107: 948. https://doi.org/10.1094/PDIS-05-22-1066-PDN
  53. Xie, J., Chen, Y., Cai, G., Cai, R., Hu, Z. & Wang, H. (2023) Tree visualization by one table (tvBOT): a web application for visualizing, modifying and annotating phylogenetic trees. Nucleic Acids Research 51: W587–W592. https://doi.org/10.1093/nar/gkad359