Skip to main content Skip to main navigation menu Skip to site footer
Responsive image
Issue: Vol. 672 No. 2: 20 Nov. 2024
Type: Article
Published: 2024-11-20
DOI: 10.11646/phytotaxa.672.2.3
Page range: 163-175
Abstract views: 114
PDF downloaded: 7

Morphological characteristics and phylogenetic analysis reveal Leccinum septentrionale sp. nov. (Boletaceae, Boletales) from China

College of Life Science, Capital Normal University, Haidian, 100048, Beijing, China.
Department of Life Sciences, National Natural History Museum of China, Tianqiaonandajie 126, Dongcheng, 100050, Beijing, China.
College of Life Science, Capital Normal University, Haidian, 100048, Beijing, China.
College of Life Science, Capital Normal University, Haidian, 100048, Beijing, China.
College of Life Science, Capital Normal University, Haidian, 100048, Beijing, China.
Boletaceae Boletales Systematics One new taxa Taxonomy Fungi

Abstract

Leccinum belongs to the Boletaceae and has attracted attention from mycologists worldwide given their ecological and economic importance. China has a high diversity of macrofungi; however, Leccinum has not been thoroughly studied due to a lack of specimen collections with associated molecular data. In this study, based on morphological evidence and phylogenetic analysis of the nucleotide sequences of four loci (ITS, LSU, rpb2, and tef1-α), one new species of Leccinum is described from Beijing, China. Morphologically, Leccinum septentrionale is characterized by its pileus yellowish-brown to brown dirty orange maculae and larger basidia with 24–40(–44) × 10–17 μm in size. This study expands the species diversity of Leccinum in Beijing and furnished valuable data support for future systematic research on Leccinum.

References

  1. Assyov, B. & Denchev, C.M. (2004) Preliminary checklist of Boletales s. str. in Bulgaria. Mycologia Balcanica 1: 195–208.
  2. Becerra, A.G. & Zak, M.R. (2011) The ectomycorrhizal symbiosis in South America: morphology, colonization, and diversity. In: Diversity and Biotechnology of ectomycorrhizae. Berlin: Springer; pp. 19–41. https://doi.org/10.1007/978-3-642-15196-5_2
  3. Binder, M. & Besl, H. (2000) 28S rDNA sequence data and chemotaxonomical analyses on the generic concept of Leccinum (Boletales). Mycologia 2000: 71–82.
  4. Corner, E.J.H. (1972) Boletus in Malaysia. Government Printer, Singapore, pp 1–263.
  5. Dai, Y.C., Cao, Y., Zhou, L.W. & Wu, S.H. (2013) Notes on the nomenclature of the most widely cultivated Ganoderma species in China. Mycosystema 32: 947–952. https://doi.org/10.13346/j.mycosystema.2013.06.010
  6. Dai, Y.C., Zhou, L.W., Yang, Z.L., Wen, H.A., BAU, T. & Li, T.H. (2010) A revised checklist of edible fungi in China. Journal of Fungi 29 (01): 1–21. https://doi.org/10.1007/s10267-010-0068-1
  7. Den Bakker, H.C., Gravendeel, B. & Kuyper, T.W. (2004b). An ITS Phylogeny of Leccinum and an Analysis of the Evolution of Minisatellite like Sequences within ITS1. Mycologia 96: 102–118. https://doi.org/10.1080/15572536.2005.11833001
  8. Den Bakker, H.C. & Noordeloos, M.E. (2005) A revision of European species of Leccinum Gray and notes on extralimital species. Persoonia 18: 511–587
  9. Den Bakker, H.C., Zuccarello, G.C., Kuyper, T.W. & Noordeloos, M.E. (2004a) Evolution and Host Specificity in the Ectomycorrhizal Genus Leccinum. New Phytologist 163 (1): 201–215. https://doi.org/10.1111/j.1469-8137.2004.01090.x
  10. Desjardin, D.E., Binder, M., Roekring, S. & Flegel, T. (2009) Spongiforma, a new genus of gasteroid boletes from Thailand. Fungal Diversity 37: 1–8. https://doi.org/10.1002/yea.1678
  11. Gao, Y., Tong, X., Zhou, H., Wang, H.Q., Li, C. & Hou, C.L. (2024) Three new species of the genus Clavulina (Hydnaceae, Cantharellales) from North China based on morphological and phylogenetic analysis. MycoKeys 108: 75–94. https://doi.org/10.3897/mycokeys.108.124004
  12. Gray, S.F., Gray, J.E. & Shury, J. (1821) A Natural Arrangement of British Plants. London UK: Cradock and Joy, 647 pp.
  13. Guindon, S., Dufayard, J.F., Lefort, V., Anisimova, M., Hordijk, W. & Gascuel, O. (2010) New algorithms and methods to estimate maximum likelihood phylogenies: Assessing the performance of PhyML 3.0. Systematic Biology 59 (3): 307–321. https://doi.org/10.1093/sysbio/syq010
  14. Halling, R.E. & Mueller GM. (2003) Leccinum (Boletaceae) in Costa Rica. Mycologia 95: 488–499. https://doi.org/10.2307/3761891
  15. Halling, R.E. (1999) New Leccinum species from Costa Rica. Kew Bulletin 54: 747–753. https://doi.org/10.2307/4110871
  16. Han, L.H., Wu, G., Horak, E., Halling, RE., Xu, J., Ndolo, E.S.T., Sato, H., Fechner, N., Sharma, Y.P. & Yang, Z.L. (2020) Phylogeny and species delimitation of Strobilomyces (Boletaceae), with an emphasis on the Asian species. Persoonia 44: 113–139. https://doi.org/10.3767/persoonia.2020.44.05
  17. Henkel, T.W., Aime, M.C., Chin, M.M., Miller, S.L., Vilgalys, R. & Smith, M.E. (2012) Ectomycorrhizal fungal sporocarp diversity and discovery of new taxa in Dicymbe monodominant forests of the Guiana Shield. Biodiversity and Conservation 21: 2195–2220. https://doi.org/10.1007/s10531-011-0166-1
  18. Hillis, D.M. & Bull, J.J. (1993) An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Systematic Biology 42: 182–192. https://doi.org/10.1093/sysbio/42.2.182
  19. Hosen, M.I., Feng, B., Wu, G., Zhu, X.T., Li, Y.C. & Yang, Z.L. (2013) Borofutus, a new genus of Boletaceae from tropical Asia: Phylogeny, morphology and taxonomy. Fungal Diversity 58: 215–226. https://doi.org/10.1007/s13225-012-0211-8
  20. Hu, Y., Karunarathna, S.C., Li, H., Galappaththi, M.C., Zhao, C.L., Kakumyan, P. & Mortimer, P.E. (2022) The impact of drying temperature on basidiospore size. Diversity 14 (4): 239.
  21. Husbands, D.R., Henkel, T.W., Bonito, G., Vilgalys, R. & Smith, M.E. (2013) New species of Xerocomus (Boletales) from the Guiana Shield, with notes on their mycorrhizal status and fruiting occurrence. Mycologia 105: 422–435. https://doi.org/10.3852/12-146
  22. Katoh, K. & Toh, H. (2010) Parallelization of the MAFFT multiple sequence alignment program. Bioinformatics 26 (15): 1899–1900. https://doi.org/10.1093/bioinformatics/btq224
  23. Kirk, P.M., Stalpers, J.A., Braun, U., Crous, P.W., Hansen, K., Hawksworth, D.L., Hyde, K., Lücking, R., Lumbsch, T.H., Rossman, A.Y., Seifert, K.A. & Stadler, M. (2013) A without-prejudice list of generic names of fungi for protection under the International Code of Nomenclature for algae, fungi, and plants. IMA fungus 4 (2): 381–443. https://doi.org/10.5598/imafungus.2013.04.02.1
  24. Lee, S.S., Alias, S.A. & Jones, E.G.B. (2012) Checklist of fungi of Malaysia. Research Pamphlet 132: 1–556.
  25. Mcnabb, R.F.R. (1968) The Boletaceae of New Zealand. New Zealand Journal of Botany 6: 137–176. https://doi.org/10.1080/0028825X.1968.10429056
  26. Meng, X., Wang, G.S., Wu, G., Wang, P.M., Yang, Z.L. & Li, Y.C. (2021) The genus Leccinum (Boletaceae, Boletales) from China based on morphological and molecular data. Journal of Fungi 7 (9): 732. https://doi.org/10.3390/jof7090732
  27. Naseer, A., Sarwar, S., Khalid, N.A., Healy, R. & Smith, M.E. (2019) Hortiboletus kohistanensis (Boletaceae), a new bolete species from temperate and subalpine oak forests of Pakistan. Phytotaxa 388 (3): 239–246. https://doi.org/10.11646/phytotaxa.388.3.3
  28. Ortiz-Santana, B. & Halling, R. (2009) A new species of Leccinum (Basidiomycota, Boletales) from Belize. Brittonia 61: 172–174. https://doi.org/10.1007/s12228-008-9067-3
  29. Posada, D. & Crandall, K.A. (1998) Modeltest: testing the model of DNA substitution. Bioinformatics 14: 817–818. https://doi.org/10.1093/bioinformatics/14.9.817
  30. Rannala, B. & Yang, Z. (1996) Probability distribution of molecular evolutionary trees: a new method of phylogenetic inference. Journal of Molecular Evolution 43: 304–311. https://doi.org/10.1007/BF02338839
  31. Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572–1574. https://doi.org/10.1093/bioinformatics/btg180
  32. Sato, H., Yumoto, T. & Murakami, N. (2007) Cryptic species and host specificity in the ectomycorrhizal genus Strobilomyces (Strobilomycetaceae). American Journal of Botany 94: 1630–1641. https://doi.org/10.3732/ajb.94.10.1630
  33. Singer, R. (1986) The Agaricales in modern taxonomy. 4th ed. Koenigstein: Koeltz Scientific Books.
  34. Smith, A.H., Thiers, H.D. & Watling, R. (1968) Notes on species of Leccinum. I. Additions to section Leccinum. Lloydia 31: 252–267.
  35. Smith, A.H, Thiers, H.D. & Watling, R.A. (1967) Preliminary account of the North American species of Leccinum, Sections Luteoscabra and Scabra. Michigan Botanical 6: 107–179.
  36. Smith, A.H. & Thiers, H.D. (1971) The Boletes of Michigan. Ann Arbor: The University of Michigan Press.
  37. Smith, M.E. & Pfister, D.H. (2009) Tuberculate ectomycorrhizae of angiosperms: The interaction between Boletus rubropunctus (Boletaceae) and Quercus species (Fagaceae) in the United States and Mexico. American Journal of Botany 96: 1665–1675. https://doi.org/10.3732/ajb.0900058
  38. Sui, X.N., Guo, M.J., Zhou, H. & Hou, C.L. (2023) Four new species of Phyllosticta from China based on morphological and phylogenetic characterization. Mycology 14 (3): 190–203. https://doi.org/10.1080/21501203.2023.2225552
  39. Stamatakis, A., Hoover, P. & Rougemont, J. (2008) A rapid bootstrap algorithm for the RAxML web servers. Systematic Biology 57 (5): 758–771. https://doi.org/10.1080/10635150802429642
  40. Stamatakis, A. (2006) RAxML-VI-HPC: Maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22 (21): 2688–2690. https://doi.org/10.1093/bioinformatics/btl446
  41. Šutara, J. (1989) The delimitation of the genus Leccinum. Česká Mykologie 43: 1–12.
  42. Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30 (12): 2725–2729. https://doi.org/10.1093/molbev/mst197
  43. Thiers, H.D. (1975) The boletes of California (online reprint of California mushrooms: a field guide to the boletes). Retrieved from the MykoWeb. Available from: http://www.mykoweb.com/boletes/index.html (accessed 19 November 2024)
  44. Thoen, D. & Bâ, A,M. (1989) Ectomycorrhizas and putative ecto-mycorrhizal fungi of Afzelia africana Sm. and Uapaca guineensis (Müll.) Arg. in southern Senegal. New Phytologist 113: 549–559. https://doi.org/10.1111/j.1469-8137
  45. Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
  46. Wang, P.M., Liu, X.B., Dai, Y.C., Horak, E., Steffen, K. & Yang, Z.L. (2018) Phylogeny and species delimitation of Flammulina: taxonomic status of winter mushroom in East Asia and a new European species identified using an integrated approach. Mycological Progress 17: 1013–1030. https://doi.org/10.1007/s11557-018-1409-2
  47. Wang, P.M., Meng, X., Yang, Z.L. & Li, Y.C. (2023) New species of Leccinum from Southwest China. Mycological Progress 22 (1). https://doi.org/10.1007/s11557-022-01848-3
  48. Wang, P.M. & Yang, Z.L. (2019) Two new taxa of the Auriscalpium vulgare species complex with substrate preferences. Mycological Progress 18: 641–652. https://doi.org/10.1007/s11557-019-01477-3
  49. Wang, Y.T., Huang, Z.H., Wang, J., Tong, Z. & Cui, G.F. (2021) The population structure and dynamic characteristics of Phellodendron amurense in Yanshan Mountains. Acta Ecologica Sinica 47 (7): 2826–2834. https://doi.org/10.5846/stxb202003300743
  50. Watling, R. (2001) Australian Boletes: their diversity and possible origins. Australian Systematic Botany 14: 407–416. https://doi.org/10.1071/SB99031
  51. Wu, F., Tohtirjap, A., Fan, L.F., Zhou, L.W., Alvarenga, R.L.M., Gibertoni, T.B. & Dai, Y.C. (2021) Global diversity and updated phylogeny of Auricularia (Auriculariales, Basidiomycota). Journal of Fungi 7: 933. https://doi.org/10.3390/jof7110933
  52. Wu, F., Yuan, Y., He, S.H., Bandara, A.R., Hyde, K.D., Malysheva, V.F., Li, D.W. & Dai, Y.C. (2015) Global diversity and taxonomy of the Auricularia auricula-judae complex (Auriculariales, Basidiomycota) Mycological Progress 14: 95. https://doi.org/10.1007/s11557-015-1113-4
  53. Wu, G., Feng, B., Xu, J.P., Zhu, X.T., Li, Y.C., Zeng, N.K., Hosen, M.I. & Yang, Z.L. (2014) Molecular phylogenetic analyses redefine seven major clades and reveal 22 new generic clades in the fungal family Boletaceae. Fungal Diversity 69: 93–115. https://doi.org/10.1007/s13225-014-0283-8
  54. Wu, G., Li, Y.C., Zhu, X.T., Zhao, K., Han, L.H., Cui, Y.Y., Li, F., Xu, J. & Yang, Z.L. (2016) One hundred noteworthy boletes from China. Fungal Diversity 81: 25–188. https://doi.org/10.1007/s13225-016-0375-8
  55. Yang, L.Z. (2011) Molecular techniques revolutionize knowledge of basidiomycete evolution. Fungal Diversity 50 (1): 47–58. https://doi.org/10.1007/s13225-011-0121-1
  56. Yang, Z.L. (2023) Flora Fungorum Sinicorum Vol. 63, Boletaceae (Ⅲ). Beijing: Science Press, pp. 1–12.
  57. Zang, M. (2013) Flora Fungorum Sinicorum. Vol. 44, Boletaceae (II). Beijing: Science Press, pp. 39–66.
  58. Zhang, X.Y. & Gao, Y.N. (2004) To design PCR primer with Oligo 6 and Primer Premier 5. China Journal of Bioinformatics 2: 15–18.
  59. Zhou, H., Cheng, G.Q., Sun, X.M., Cheng, R.Y., Zhang, H.L., Dong, Y.M. & Hou, C.L. (2022) Three new species of Candolleomyces (Agaricomycetes, Agaricales, Psathyrellaceae) from the Yanshan Mountains in China. MycoKeys 88: 109–121. https://doi.org/10.3897/mycokeys.88.81437
  60. Zhou, H. & Hou, C.L. (2019) Three new species of Diaporthe from China based on morphological characters and DNA sequence data analyses. Phytotaxa 422 (2): 157–174. https://doi.org/10.11646/phytotaxa.422.2.3
  61. Zhou, H., Wang, Q.T., Tong, X. & Hou, C.L. (2021) Phylogenetic analysis of Engleromyces sinensis and identification of cytochalasin D from culture. Mycological Progress 20 (10): 1343–1352. https://doi.org/10.1007/s11557-021-01739-z