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Issue: Vol. 668 No. 1: 10 Oct. 2024
Type: Article
Published: 2024-10-10
DOI: 10.11646/phytotaxa.668.1.2
Page range: 11-43
Abstract views: 164
PDF downloaded: 7

First Report on Taxonomic Significance of Foliar Microanatomy of Lamiaceae Taxa from Pakistan; A Correct Tool for Taxa Authentication

Department of Plant Sciences, Quaid-i-Azam University, 45320 Islamabad
Department of Plant Sciences, Quaid-i-Azam University, 45320 Islamabad
Department of Plant Sciences, Quaid-i-Azam University, 45320 Islamabad
Department of Plant Sciences, Quaid-i-Azam University, 45320 Islamabad. Department of Botany, University of Chakwal, Chakwal, 48800, Pakistan.
Department of Plant Sciences, Quaid-i-Azam University, 45320 Islamabad. Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences (CAS) Lanzhou, China.
Department of Environment & Biodiversity, University of Salzburg, Austria.
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
Department of Agronomy, Faculty of Agriculture and Environment, The Islamia University of Bahawalpur 63100, Pakistan. Department of Life Sciences, Western Caspian University, Baku, Azerbaijan.
Lamiaceae species scanning electron microscopy Systematics Taxonomy Eudicots

Abstract

The foliar epidermal, stomatal and trichome properties are frequently used as plant microscopic attributes from a taxonomic or ecological perspective. Among the angiosperm families, the Lamiaceae is one of the most taxonomically difficult, economically significant, and medicinally important families. To better understand the foliar microanatomy of Lamiaceae taxa obtained from Northern Pakistan, this study attempts to determine the variational and diagnostic features of 27 species that were analyzed using light microscopy (LM) and scanning electron microscopy (SEM). UPGMA cluster analysis, principal component analysis (PCA) and semantic differential chart (SDC) were used to characterize, visualize, and compare the leaf micromorphology across all species. Three different types of epidermal cell types were examined on both foliar surfaces i.e., irregular, hexagonal, and polygonal. Anticlinal wall pattern also shows variation. Anomocytic stomata is dominant in 8 studied species followed by paractic in 5 species, anisocytic in 4 species and diacytic in 2 species. The trichomes could be broadly classified into two groups, glandular trichomes (GTs) and non-glandular trichomes (NGTs). Lamiaceae has two types of glandular trichomes, small capitate and large peltate, which differ in size, structure, and distribution. In present research five distinct forms of non-glandular trichomes are unicellular, multicellular, stellate, branched, and unbranched trichomes. The results that characterized, illustrated, and contrasted the leaf anatomical characteristics of the Lamiaceae taxa can be better understood with the aid of statistical analysis. Future research will depend on these findings to improve the systematics of Lamiaceae taxa. These characteristics have a large taxonomic potential when considered as a whole.

References

  1. Abdullah, M., Ullah, Z., Shah, S.M.I., Ahmad, M., Khan, A., Shah, H., Ullah, A., Ahmad, S., Albeshr, M.F., Emmanuel, O. & Ullah, H. (2024) Systematic implication of trichome morphology in alpine flora from Hindukush Mountain range. Genetic Resources and Crop Evolution 2024. https://doi.org/10.1007/s10722-024-02079-z
  2. Abu-Asab, M.S. & Cantino, P.D. (1987) Phylogenetic implications of leaf anatomy in subtribe Melittidinae (Labiatae) and related taxa. Journal of the Arnold Arboretum 68: 1–34. https://doi.org/10.5962/p.185940
  3. Akinsulire, O.P., Oladipo, O.T., Akinkunmi, O.C., Adeleye, O.E. & Adelalu, K.F. (2020) Leaf and Petiole Micro-Anatomical Diversities in Some Selected Nigerian Species of Loefl.: the Significance in Species Identification at Vegetative State. Acta Biologica Marisiensis 3 (1): 15–29. https://doi.org/10.2478/abmj-2020-0002
  4. Al-Shehbaz, I., Beilstein, M. & Kellogg, E. (2006) Systematics and phylogeny of the Brassicaceae (Cruciferae): an overview. Plant systematics and evolution 259: 89–120. https://doi.org/10.1007/s00606-006-0415-z
  5. Amelunxen, F. (1964) Elektronenmikroskopische Untersuchungen an den Drüsenhaaren von Mentha piperita L. Planta medica 12 (02): 121–139. https://doi.org/10.1055/s-0028-1100158
  6. Amelunxen, F., Wahlig, T. & Arbeiter, H. (1969) Über den Nachweis des ätherischen Öls in isolierten Drüsenhaaren und Drüsenschuppen von Mentha piperita L. Zeitschrift für Pflanzenphysiologie 61 (1): 68–72.
  7. Arceo, A., Tham, M., Guven, G., MacLean, H.L. & Saxe, S. (2021) Capturing variability in material intensity of single-family dwellings: A case study of Toronto, Canada. Resources, Conservation and Recycling 175: 105885. https://doi.org/10.1016/j.resconrec.2021.105885
  8. Ascensao, L., Marques, N. & Pais, M.S. (1997) Peltate glandular trichomes of Leonotis leonurus leaves: ultrastructure and histochemical characterization of secretions. International Journal of Plant Sciences 158 (3): 249–258. https://doi.org/10.1086/297436
  9. Ascensão, L., Mota, L., DE, M. & Castro, M. (1999) Glandular Trichomes on the Leaves and Flowers of Plectranthus ornatus: Morphology, Distribution and Histochemistry. Annals of Botany 84 (4): 437–447. https://doi.org/10.1006/anbo.1999.0937
  10. Ascensão, L. & Pais, M. (1998) The leaf capitate trichomes of Leonotis leonurus: Histochemistry, Ultrastructure and Secretion. Annals of Botany 81 (2): 263–271. https://doi.org/10.1006/anbo.1997.0550
  11. Ashfaq, S., Ahmad, M., Zafar, M., Sultana, S., Bahadur, S., Ullah, F., Zaman, W., Ahmed, S.N. & Nazish, M. (2019) Foliar micromorphology of Convolvulaceous species with special emphasis on trichome diversity from the arid zone of Pakistan. Flora 255: 110–124. https://doi.org/10.1016/j.flora.2019.04.007
  12. Atalay, Z., Celep, F., Bara, F. & Doğan, M. (2016) Systematic significance of anatomy and trichome morphology in Lamium (Lamioideae; Lamiaceae). Flora - Morphology, Distribution, Functional Ecology of Plants 225: 60–75. https://doi.org/10.1016/j.flora.2016.10.006
  13. Azizian, D. & Cutler, D. (1982) Anatomical, cytological and phytochemical studies on Phlomis L. and Eremostachys Bunge (Labiatae). Botanical journal of Linnaean Society 85 (4): 249–281. https://doi.org/10.1111/j.1095-8339.1982.tb00373.x
  14. Badry, M.O., Osman, A.K., Aboulela, M., Gafar, S. & Nour, I.H. (2024) Taxonomic implications of normal and abnormal stomatal complexes in Indigofera L. (Indigofereae, Faboideae, Fabaceae). Protoplasma 261: 991–1021. https://doi.org/10.1007/s00709-024-01951-0
  15. Beilstein, M.A., Al‐Shehbaz, I.A. & Kellogg, E.A. (2006) Brassicaceae phylogeny and trichome evolution. American Journal of Botany 93 (4): 607–619. https://doi.org/10.3732/ajb.93.4.607
  16. Belhadj, S., Derridj, A., Aigouy, T., Gers, C., Gauquelin, T. & Mevy, J.P. (2007) Comparative morphology of leaf epidermis in eight populations of Atlas pistachio (Pistacia atlantica Desf., Anacardiaceae). Microscopy Research and Technique 70 (10): 837–846. https://doi.org/10.1002/jemt.20483
  17. Benjamin, E.J., Virani, S.S., Callaway, C.W., Chamberlain, A.M., Chang, A.R., Cheng, S., Chiuve, S.E., Cushman, M., Delling, F.N., Deo, R., de Ferranti, S.D., Ferguson, J.F., Fornage, M., Gillespie, C., Isasi, C.R., Jiménez, M.C., Jordan, L.C., Judd, S.E., Lackland, D., Lichtman, J.H., Lisabeth, L., Liu, S., Longenecker, C.T., Lutsey, P.L., Mackey, J.S., Matchar, D.B., Matsushita, K., Mussolino, M.E., Nasir, K., O’Flaherty, M., Palaniappan, L.P., Pandey, A., Pandey, D.K., Reeves, M.J., Ritchey, M.D., Rodriguez, C.J., Roth, G.A., Rosamond, W.D., Sampson, U.K.A., Satou, G.M., Shah, S.H., Spartano, N.L., Tirschwell, D.L., Tsao, C.W., Voeks, J.H., Willey, J.Z., Wilkins, J.T., Wu, J.H.Y., Alger, H.M., Wong, S.S. & Muntner, P. (2018) Heart disease and stroke statistics—2018 update: a report from the American Heart Association. Circulation 137 (12): e67–e492. https://doi.org/10.1161/CIR.0000000000000558
  18. Bhatt, A., Naidoo, Y. & Nicholas, A. (2010) The foliar trichomes of Plectranthus laxiflorus Benth [Lamiaceae]: an important medicinal plant. New Zealand Journal of Botany 48 (2): 55–61. https://doi.org/10.1080/0028825X.2010.482958
  19. Bini Maleci, L. & Servettaz, O. (1991) Morphology and distribution of trichomes in Italian species of Teucrium sect. Chamaedrys (Labiatae)—a taxonomical evaluation. Plant Systematics & Evolution 174: 83–91. https://doi.org/10.1007/BF00937696
  20. Abu-Asab, M.S. & Cantino, P.D. (1987) Phylogenetic implications of leaf anatomy in subtribe Melittidinae (Labiatae) and related taxa. Journal of the Arnold Arboretum 68: 1–34.
  21. https://doi.org/10.5962/p.185940
  22. Bosabalidis, A. (1990) Glandular trichomes in Satureja thymbra leaves. Annales Botany 65 (1): 71–78. https://doi.org/10.1093/oxfordjournals.aob.a087910
  23. Bosabalidis, A. & Tsekos, I. (1982) Ultrastructural studies on the secretory cavities of Citrus deliciosa Ten. II. Development of the essential oil-accumulating central space of the gland and process of active secretion. Protoplasma 112: 63–70. https://doi.org/10.1007/BF01280216
  24. Bosabalidis, A. & Tsekos, I. (1984) Glandular hair formation in Origanum species. Annalas of Botany 53 (4): 559–563. https://doi.org/10.1093/oxfordjournals.aob.a086719
  25. Botanica, A. (2009) Micromorphological studies of Lallemantia L. (Lamiaceae) species growing in Turkey. Acta Biologica Cracoviensia Series Botanica 51 (1): 45–54.
  26. Briquet, J. (1895) Notes sur la flore du massif de Platé. R. Burkhardt. https://doi.org/10.3406/globe.1895.1984
  27. Candido, D.S., Claro, I.M., De Jesus, J.G., Souza, W.M., Moreira, F.R., Dellicour, S., Mellan, T.A., Du Plessis, L., Pereira, R.H. & Sales, F.C. (2020) Evolution and epidemic spread of SARS-CoV-2 in Brazil. Science 369 (6508): 1255–1260. https://doi.org/10.1126/science.abd2161
  28. Cantino, P.D. (1990) The phylogenetic significance of stomata and trichomes in the Labiatae and Verbenaceae. Journal of Arnold Arboretum 71: 323–370. https://doi.org/10.5962/p.184532
  29. Casella, F., Vurro, M., Valerio, F., Perrino, E.V., Mezzapesa, G.N. & Boari, A. (2023) Phytotoxic Effects of Essential Oils from Six Lamiaceae Species. Agronomy 13: 257. https://doi.org/10.3390/agronomy13010257
  30. Celep, F., Kahraman, A., Atalay, Z., Dogan, M. (2011) Morphology, anatomy and trichome properties of’Lamium truncatum’Boiss.(Lamiaceae) and their systematic implications. Australian Journal of Crop Sciences. 5 (2): 147–153.
  31. Clarke, J. (1960) Preparation of leaf epidermis for topographic study. Stain Technology 35 (1): 35–39. https://doi.org/10.3109/10520296009114713
  32. Cousins, D.J. (Ed.) (1994) Medicinal, essential oil, culinary herb and pesticidal plants of the Labiatae 1973-1993. CAB International, London, 354 pp.
  33. de Oliveira, A.B., de Mendonça, M.S. & Meira, R.M. (2013) Anatomy of vegetative organs of Scutellaria agrestis, a medicinal plant cultivated by riverine populations of the Brazilian Amazon. Revista Brasileira de Farmacognosi 23 (3): 386–397. https://doi.org/10.1590/S0102-695X2013005000034
  34. Dehshiri, M., Azadbakht, M. (2012) Anatomy of Iranian species Teucrium polium (Lamiaceae). Journal of Biology and Today’s World 1 (2): 93–98.
  35. Dudai, N., Werker, E., Putievsky, E., Ravid, U., Palevitch, D. & Halevy, A. (1988) Glandular hairs and essential oils in the leaves and flowers of Majorana syriaca. Israel Journal of Botany 37 (1): 11–18.
  36. Erdtman, G. (1945) Pollen morphology and plant taxonomy. Svensk botanisk tidskrift 38: 163–168.
  37. Fajuke, A.A., Makinde, A.M., Oloyede, F.A. & Akinloye, J.A. (2018) Comparative epidermal anatomical studies in six taxa of genus Nephrolepis Swart in Nigeria. Tropical Plant Reseearh 5 (1): 19. https://doi.org/10.22271/tpr.2018.v5.i1.004
  38. Gairola, S., Naidoo, Y., Bhatt, A. & Nicholas, A. (2009) An investigation of the foliar trichomes of Tetradenia riparia (Hochst.) Codd [Lamiaceae]: an important medicinal plant of Southern Africa. Flora-Morphology, Distribution, Functional Ecology of Plants 204 (4): 325–330. https://doi.org/10.1016/j.flora.2008.04.002
  39. Gerçek, Y.C., Şahin, A.A., Bayram, N.E., Çelik, S., Sefalı, A., Gıdık, B., Öz, G.C. & Pınar, N.M. (2022) Anatomy, trichome micromorphology and phytochemical profile of Stachys rizeensis R.Bhattacharjee from Turkey. South africa journal of botany 149: 19–28. https://doi.org/10.1016/j.sajb.2022.05.046
  40. Gul, S., Ahmad, M., Zafar, M., Bahadur, S., Sultana, S., Ashfaq, S., Ullah, F., Kilic, O., Hassan, Fu. & Siddiq, Z. (2019) Foliar epidermal anatomy of Lamiaceae with special emphasis on their trichomes diversity using scanning electron microscopy. Microscopy reearch & technique 82 (3): 206–223. https://doi.org/10.1002/jemt.23157
  41. Hanlidou, E., Kokkini, S., Bosabalidis, A. & Bessière, J-M. (1991) Glandular trichomes and essential oil constituents of Calamintha menthifolia (Lamiaceae). Plant Systematics and Evolution 177: 17–26. https://doi.org/10.1007/BF00937823
  42. Haruna, H. & Ashir, H. (2017) leaf epidermal members of the. Journal of Pure & Applied Sciences 10 (1): 670–675.
  43. Heinrich, G., Schultze, W., Pfab, I. & Boettger, M. (1983) The site of essential oil biosynthesis in Poncirus trifoliata and Monarda fistulosa. Physiologie Vegetale 21: 257–268.
  44. Herman, P. (1998) The leaf anatomy of two Clerodendrum species (Verbenaceae). South African Journal of Botany 64 (4): 246–249. https://doi.org/10.1016/S0254-6299(15)30888-7
  45. Husain, S., Marin, P., Diklic, N. & Patcovic, B. (1989) micromorphological and phytochemical studies in. Pakistan Journal of Botany 21 (2): 210–217.
  46. Inamdar, J. & Bhatt, D. (1972a) Epidermal structure and ontogeny of stomata in vegetative and reproductive organs of Ephedra and Gnetum. Annals of Botany 36 (5): 1041–1046. https://doi.org/10.1093/oxfordjournals.aob.a084647
  47. Inamdar, J. & Bhatt, D. (1972b) Structure and development of stomata in some Labiatae. Annal Botany 36 (2): 335–344. https://doi.org/10.1093/oxfordjournals.aob.a084593
  48. James, O.E., Green, B.O., Ajuru, M.G. & Wilson, V. (2021) Foliar epidermal anatomy and its taxonomic implications within the family Euphorbiaceae in The Niger Delta region of Nigeria. Journal of Frontiers in Life Science Research 1 (1): 48–55. https://doi.org/10.53294/ijflsr.2021.1.1.0036
  49. Jurišić, G.R., Vladimir-Knežević, S., Kremer, D., Kalodera, Z. & Vuković, J. (2007) Trichome micromorphology in Teucrium (Lamiaceae) species growing in Croatia. Biologia 62: 148–156. https://doi.org/10.2478/s11756-007-0023-6
  50. Kahraman, A., Celep, F. & Dogan, M. (2010a) Anatomy, trichome morphology and palynology of Salvia chrysophylla Stapf (Lamiaceae). South African Journal of Botany 76 (2): 187–195. https://doi.org/10.1016/j.sajb.2009.10.003
  51. Kahraman, A., Dogan, M., Celep, F., Akaydin, G. & Koyuncu, M.J.NJ.O.B. (2010b) Morphology, anatomy, palynology and nutlet micromorphology of the rediscovered Turkish endemic Salvia ballsiana (Lamiaceae) and their taxonomic implications. Nordic Journal of Botany 28 (1): 91–99. https://doi.org/10.1111/j.1756-1051.2009.00384.x
  52. Karabourniotis, G., Liakopoulos, G., Nikolopoulos, D. & Bresta, P. (2020) Protective and defensive roles of non-glandular trichomes against multiple stresses: structure-function coordination. Journal of Forestry Research 31 (1): 1–12. https://doi.org/10.1007/s11676-019-01034-4
  53. Kaya, A., Demirci, B. & Baser, K. (2007) Micromorphology of glandular trichomes of Nepeta congesta Fisch. & Mey. var. congesta (Lamiaceae) and chemical analysis of the essential oils. South Africa Journal of Botany 73 (1): 29–34. https://doi.org/10.1016/j.sajb.2006.05.004
  54. Khan, R., Abidin, S.Z.U., Ahmad, M., Zafar, M., Liu, J., Jamshed, S. & Kiliç, Ö. (2019) Taxonomic importance of SEM and LM foliar epidermal micro-morphology: A tool for robust identification of gymnosperms. Flora 255: 42–68. https://doi.org/10.1016/j.flora.2019.03.016
  55. Klich, M.G. (2000) Leaf variations in Elaeagnus angustifolia related to environmental heterogeneity. Environmental and Experimental Botany 44 (3): 171–183. https://doi.org/10.1016/S0098-8472(00)00056-3
  56. Liakoura, V., Stavrianakou, S., Liakopoulos, G., Karabourniotis, G. & Manetas, Y. (1999) Effects of UV-B radiation on Olea europaea: comparisons between a greenhouse and a field experiment. Tree physiology 19 (13): 905–908. https://doi.org/10.1093/treephys/19.13.905
  57. Maffei, M. & Codignola, A. (1990) Photosynthesis, photorespiration and herbicide effect on terpene production in peppermint (Mentha piperita L.). Journal of Essential Oil Research 2 (6): 275–286. https://doi.org/10.1080/10412905.1990.9697886
  58. Mannethody, S. & Purayidathkandy, S. (2018) Trichome micromorphology and its systematic significance in Asian Leucas (Lamiaceae). Flora 242: 70–78. https://doi.org/10.1016/j.flora.2018.03.007
  59. Marin, P.D., Petković, B. & Duletić, S. (1994) Nutlet sculpturing of selected Teucrium species (Lamiaceae): a character of taxonomic significance. Plant Systematics Evolution 192: 199–214. https://doi.org/10.1007/BF00986252
  60. Metcalfe, C.R., (1968) Current developments in systematic plant anatomy. In: Heywood, V.H. (Ed.) Modern methods in plant taxonomy. Academic Press, London & New York, pp. 45–57.
  61. Stefanaki, A. & van Andel, T. (2021) Mediterranean aromatic herbs and their culinary use. In: Aromatic Herbs in Food. Academic Press, pp. 93–121. https://doi.org/10.1016/B978-0-12-822716-9.00003-2
  62. Mohd Rozar, N., Sidik, M.H., Razik, M.A., Ahmad, Kamaruddin, S., Rozar, M.K.A.M., Usman, I. & Alown, B.E. (2023) A hierarchical cluster analysis of port performance in Malaysia. Maritime Business Review 8 (3): 194–208. https://doi.org/10.1108/MABR-07-2020-0040
  63. Moon, H-K., Hong, S-P., Smets, E. & Huysmans, S. (2009) Phylogenetic significance of leaf micromorphology and anatomy in the tribe Mentheae (Nepetoideae: Lamiaceae). Botanical Journal of Linnaean Society 160 (2): 211–231. https://doi.org/10.1111/j.1095-8339.2009.00979.x
  64. Mun, H.G. & Hong, SP (2003) The taxonomic consideration of leaf epidermal microstructure in Lycopus L. (Mentheae, Lamiaceae). Korean Journal of Plant Taxonomy 33 (2): 151–164. https://doi.org/10.11110/kjpt.2003.33.2.151
  65. Navarro, T. & El Oualidi, J. (1999) Trichome morphology in Teucrium L.(Labiatae). A taxonomic review. Anales del Jardín Botánico de Madrid 2: 277–297. https://doi.org/10.3989/ajbm.1999.v57.i2.203
  66. Navarro, T. & El Oualidi, J. (2000) Synopsis of Teucrium L.(Labiatae) in the Mediterranean region and surrounding areas. Flora Mediterranea 10: 349–363.
  67. Nazir, M.S., Wahjoedi, B.A., Yussof, A.W. & Abdullah, M.A.J.B. (2013) Eco-friendly extraction and characterization of cellulose from oil palm empty fruit bunches. Bioresource 8 (2): 2161–2172. http://dx.doi.org/10.15376/biores.8.2.2161-2172
  68. Nazish, M., Zafar, M., Ahmad, M., Sultana, S., Ullah, R., Alqahtani, A.S., Ullah, F., Ahmad, S., Ashfaq, S. & Ullah, F. (2019) Palyno‐morphological investigations of halophytic taxa of Amaranthaceae through SEM from Salt range of Northern Punjab, Pakistan. Microscopy Research & Technique 82 (3): 304–316. https://doi.org/10.1002/jemt.23173
  69. Osman, A. (2012) Trichome micromorphology of Egyptian Ballota (Lamiaceae) with emphasis on its systematic implication. Pakistan Journal of Botany 44 (1): 33–46
  70. Perrino, E.V., Mahmoud, Z.N.A., Valerio, F., Tomaselli, V., Wagensommer, R.P. & Trani, A. (2023) Synecology of Lagoecia cuminoides L. in Italy and evaluation of functional compounds presence in its water or hydroalcoholic extracts. Scientific Reports 13: 20906. https://doi.org/10.1038/s41598-023–48065-w
  71. Perveen, A. & Qaiser, M.J.P.J.B. (2004) Pollen morphology of the family Labiatae from Pakistan. Pakistan Journal of Botany 35 (5): 671–694.
  72. Putievsky, E., Ravid, U. & Dudai, N. (1988) Phenological and seasonal influences on essential oil of a cultivated clone of Origanum vulgare L. Journal of the Science of Food and Agriculture 43 (3): 225–228. https://doi.org/10.1002/jsfa.2740430304
  73. Rashid, N., Zafar, M., Ahmad, M., Khan, M.A., Malik, K., Sultana, S. & Shah, S.N. (2019) Taxonomic significance of leaf epidermis in tribe Trifolieae L. (Leguminosae; Papilionoideae) in Pakistan. Plant Biosystem 153 (3): 406–416. https://doi.org/10.1080/11263504.2018.1492995
  74. Raza, J., Ahmad, M., Zafar, M., Yaseen, G., Sultana, S. & Majeed, S. (2022) Systematic significance of seed morphology and foliar anatomy among Acanthaceous taxa. Biologia 77 (11): 3125–3142. https://doi.org/10.1007/s11756-022-01137-0
  75. Rozar, N.M., Sidik, M.H., Razik, M.A., Kamaruddin, S.A., Rozar, M.K.A.M., Usman, I. & Alown, B.E. (2022) A hierarchical cluster analysis of port performance in Malaysia. Maritime Business Review 8 (3): 194–208. https://doi.org/10.1108/MABR-07-2020-0040
  76. Rudall, P. (1980) Leaf anatomy of the subtribe Hyptidinae (Labiatae). Botanical journal of Linnaean Society 80 (4): 319–340. https://doi.org/10.1111/j.1095-8339.1980.tb01667.x
  77. Salmaki, Y., Zarre, S., Jamzad, Z. & Bräuchler, C. (2009) Trichome micromorphology of Iranian Stachys (Lamiaceae) with emphasis on its systematic implication. Flora-Morphology, Distribution, Functional Ecology of Plants 204 (5): 371–381. https://doi.org/10.1016/j.flora.2008.11.001
  78. Sanoj, E. & Deepa, P. (2021) Micromorphological variations of trichomes in the genus Ocimum L. Plant science today 8 (3): 429–436. https://doi.org/10.14719/pst.2021.8.3.1006
  79. Satil, F., Ünal, M. & Hopa, E. (2007) Comparative morphological and anatomical studies of Hymenocrater bituminosus Fisch. & CA Mey.(Lamiaceae) in Turkey. Turkish Journal of Botany 31 (3): 269–275.
  80. Schilmiller, A.L., Last, R.L. & Pichersky, E. (2008) Harnessing plant trichome biochemistry for the production of useful compounds. The Plant Journal 54 (4): 702–711. https://doi.org/10.1111/j.1365-313X.2008.03432.x
  81. Schreuder, M.D., Brewer, C.A. & Heine, C. (2001) Modelled influences of non-exchanging trichomes on leaf boundary layers and gas exchange. Journal of Theoretical Biology 210 (1): 23–32. https://doi.org/10.1006/jtbi.2001.2285
  82. Serrato-Valenti, G., Bisio, A., Cornara, L. & Ciarallo, G. (1997) Structural and histochemical investigation of the glandular trichomes of Salvia aurea L. leaves, and chemical analysis of the essential oil. Annales Botany 79 (3): 329–336. https://doi.org/10.1006/anbo.1996.0348
  83. Shah, S.N., Ahmad, M., Zafar, M., Malik, K., Rashid, N., Ullah, F., Zaman, W. & Ali, M. (2018) A light and scanning electron microscopic diagnosis of leaf epidermal morphology and its systematic implications in Dryopteridaceae: Investigating 12 Pakistani taxa. Micron 111: 36–49. https://doi.org/10.1016/j.micron.2018.05.008
  84. Shah G, Naidu, A. (1983) Trichomes on leaves of some Lamiaceae. Geophytologist 13 (2): 165–176.
  85. Sharma, K.K. (2006) Optics: principles and applications. Elsevier. Academic Press, 656 pp.
  86. El‐Gazzar, A. & Watson, L. (1970) A taxonomic study of Labiatae and related genera. New Phytologist 69 (2): 451–486. https://doi.org/10.1111/j.1469-8137.1970.tb02443.x
  87. Stant, M.Y. (1973) The role of the scanning electron microscope in plant anatomy. Kew Bulletin 28: 105–115. https://doi.org/10.2307/4117068
  88. Frezza, C., Venditti, A., Serafini, M. & Bianco, A. (2019) Phytochemistry, chemotaxonomy, ethnopharmacology, and nutraceutics of Lamiaceae. Studies in natural products chemistry 62: 125–178. https://doi.org/10.1016/B978-0-444-64185-4.00004-6
  89. Sufyan, M., Badshah, I., Ahmad, M., Zafar, M., Bahadur, S. & Rashid, N. (2018) Identification of medicinally used Flora using pollen features imaged in the scanning electron microscopy in the lower Margalla Hills Islamabad Pakistan. Microscopy and Microanalysis 24 (3): 292–299. https://doi.org/10.1017/S1431927618000326
  90. Tissier, A. (2012) Glandular trichomes: what comes after expressed sequence tags? The Plant Journal 70 (1): 51–68. https://doi.org/10.1111/j.1365-313X.2012.04913.x
  91. Ullah, F., Zafar, M., Ahmad, M., Dilbar, S., Shah, S.N., Sohail, A., Zaman, W., Iqbal, M., Bahadur, S. & Tariq, A. (2018) Pollen morphology of subfamily Caryophylloideae (Caryophyllaceae) and its taxonomic significance. Microscopy Research and Technique 81 (7): 704–715. https://doi.org/10.1002/jemt.23026
  92. Vrachnakis, T. (2003) Trichomes of Origanum dictamnus L. (Labiatae). Phyton; Annales Rei Botanicae 43 (1): 109–133.
  93. Wagner, G., Wang, E. & Shepherd, R. (2004) New Approaches for Studying and Exploiting an Old Protuberance, the Plant Trichome. Annals of Botany 93 (1): 3–11. https://doi.org/10.1093/aob/mch011
  94. Webster, G., Del-Arco-Aguilar, M. & Smith, B. (1996) Systematic distribution of foliar trichome types in Croton (Euphorbiaceae). Botanical journal of linnaean society 121 (1): 41–57. https://doi.org/10.1006/bojl.1996.0023
  95. Werker, E. (1993) Function of essential oil‐secreting glandular hairs in aromatic plans of Lamiacea—a review. Flavour and Fragrance Journal 8 (5): 249–255. https://doi.org/10.1002/ffj.2730080503
  96. Werker, E., Ravid, U. & Putievsky, E. (1985) Structure of glandular hairs and identification of the main components of their secreted material in some species of the Labiatae. Isrrael Journal of Plant Sciences 34 (1): 31–45.
  97. Zaman, W., Ullah, F., Parmar, G., Saqib, S., Ayaz, A. & Park, S. (2022a) Foliar micromorphology of selected medicinal Lamiaceae taxa and their taxonomic implication using scanning electron microscopy. Microscopy Research and Technique 85 (9): 3217–3236. https://doi.org/10.1002/jemt.24179