Skip to main content Skip to main navigation menu Skip to site footer
Responsive image
Issue: Vol. 660 No. 1: 16 Jul. 2024
Type: Article
Published: 2024-07-16
DOI: 10.11646/phytotaxa.660.1.5
Page range: 55-64
Abstract views: 50
PDF downloaded: 2

Appendopyricularia guttulata sp. nov., a lignicolous freshwater fungus from Dianchi Lake in Yunnan, China

College of Agriculture and Biological Science, Dali University, Dali 671003, Yunnan, P.R. China
Engineering and Research Center for Southwest Biopharmaceutical Resource of National Education Ministry of China, Guizhou University, Guiyang, 550025, Guizhou, P.R. China
College of Agriculture and Biological Science, Dali University, Dali 671003, Yunnan, P.R. China, Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand, School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand
College of Agriculture and Biological Science, Dali University, Dali 671003, Yunnan, P.R. China
College of Agriculture and Biological Science, Dali University, Dali 671003, Yunnan, P.R. China, Co-Innovation Center for Cangshan Mountain and Erhai Lake Integrated Protection and Green Development of Yunnan Province, Dali University, Dali 671003, Yunnan, P.R. China
1 new species Barbatosphaeriaceae freshwater fungi phylogeny taxonomy Fungi

Abstract

During an investigation into species diversity of lignicolous freshwater fungi from the plateau lakes in Yunnan Province, China, a hyphomycetous fungus was collected from Dianchi Lake. The new isolate was placed in Appendopyricularia as a sister taxon of Appendopyricularia juncicola within Barbatosphaeriaceae based on the phylogenetic analysis of combined ITS and LSU sequence data. The fungus is characterized by acrogenous, fusoid, guttulate conidia that are 3–4 septate and have a flexuous central apical appendage. It exhibits more pronounced guttulate appendages and more prominent denticle sporulation structure. Therefore, Appendopyricularia guttulata is introduced as a new species based on morphological characteristics and phylogenetic evidence. The detailed description and illustrations of the new species are provided, as well as the morphological comparison with similar taxa.

References

  1. Bao, D.F., Hyde, K.D., Maharachchikumbura, S.S.N., Perera, R.H., Thiyagaraja, V., Hongsanan, S., Wanasinghe, D.N., Shen, H.W., Tianm, X.G., Yang, L.Q., Nalumpang, S. & Luo, Z.L. (2023) Taxonomy, phylogeny and evolution of freshwater Hypocreomycetidae (Sordariomycetes). Fungal Diversity 121: 1–94. https://doi.org/10.1007/s13225-023-00521-8
  2. Bao, D.F., Luo, Z.L., Liu, J.K., Bhat, D.J., Sarunya, N., Li, W.L., Su, H.Y. & Hyde, K.D. (2018) Lignicolous freshwater fungi in China III: New species and record of Kirschsteiniothelia from northwestern Yunnan Province. Mycosphere 9: 755–768. https://doi.org/10.5943/mycosphere/9/4/4
  3. Cai, L., Jeewon, R. & Hyde, K.D. (2006) Phylogenetic investigations of Sordariaceae based on multiple gene sequences and morphology. Mycological Research 110: 137–150. https://doi.org/10.1016/j.mycres.2005.09.014
  4. Calabon, M.S., Hyde, K.D., Jones, E.B.G., Bao, D.F., Bhunjun, C.S., Phukhamsakda, C., Shen, H.W., Gentekaki, E., Al, Sharie, A.H., Barros, J., Chandrasiri, K.S.U., Hu, D.M., Hurdeal, V.G., Rossi, W., Valle, L.G., Zhang, H., Figueroa, M., Raja, H.A., Seena, S., Song, H.Y., Dong, W., El-Elimat, T., Leonardi, M., Li, Y., Li, Y.J., Luo, Z.L., Ritter, C.D., Strongman, D.B., Wei, M.J. & Balasuriya, A. (2023) Freshwater fungal biology. Mycosphere 14: 195–413. https://doi.org/10.5943/mycosphere/14/1/4
  5. Calabon, M.S., Hyde, K.D., Gareth Jones, E.B., Luo, Z.L., Dong, W., Hurdeal, V.G., Gentekaki, E., Rossi, W., Leonardi, M., Thiyagaraja, V., Lestari, A.S., Shen, H.W., Bao, D.F., Boonyuen, N. & Zeng, M. (2022) Freshwater fungal numbers. Fungal Diversity 114: 3–235. https://doi.org/10.1007/s13225-022-00503-2
  6. Capella-Gutiérrez, S., Silla-Martínez, J.M. & Gabaldón, T. (2009) trimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses. Bioinformatics 25: 1972–1973. https://doi.org/10.1093/bioinformatics/btp348
  7. Crous, P.W., Akulov, A., Balashov, S., Boers, J., Braun, U., Castillo, J., Delgado, M.A., Denman, S., Erhard, A., Gusella, G., Jurjević, Ž., Kruse, J., Malloch, D.W., Osieck, E.R., Polizzi, G., Schumacher, R.K., Slootweg, E., Starink-Willemse, M., Van, Iperen, A.L., Verkley, G.J.M. & Groenewald, J.Z. (2023) New and interesting fungi. 6. Fungal Systematics and Evolution 11: 109–156. https://doi.org/10.3114/fuse.2023.11.09
  8. Crous, P.W., Boers, J., Holdom, D., Osieck, E.R., Steinrucken, T.V., Tan, Y.P., Vitelli, J.S., Shivas, R.G., Barrett, M., Boxshall, A.G., Broadbridge, J., Larsson, E., Lebel, T., Pinruan, U., Sommai, S., Alvarado, P., Bonito, G., Decock, C.A., De la Peña-Lastra, S., Delgado, G., Houbraken, J., Maciá-Vicente, J.G., Raja, H.A., Rigueiro-Rodríguez, A., Rodríguez, A., Wingfield, M.J., Adams, S.J., Akulov, A., AL-Hidmi, T., Antonín, V., Arauzo, S., Arenas, F., Armada, F., Aylward, J., Bellanger, J.-M., Berraf-Tebbal, A., Bidaud, A., Boccardo, F., Cabero, J., Calledda, F., Corriol, G., Crane, J.L., Dearnaley, J.D.W., Dima, B., Dovana, F., Eichmeier, A., Esteve-Raventós, F., Fine, M., Ganzert, L., García, D., Torres-Garcia, D., Gené, J., Gutiérrez, A., Iglesias, P., Istel, Ł., Jangsantear, P., Jansen, G.M., Jeppson, M., Karun, N.C., Karich, A., Khamsuntorn, P., Kokkonen, K., Kolařík, M., Kubátová, A., Labuda, R., Lagashetti, A.C., Lifshitz, N., Linde, C., Loizides, M., Luangsa-ard, J.J., Lueangjaroenkit, P., Mahadevakumar, S., Mahamedi, A.E., Malloch, D.W., Marincowitz, S., Mateos, A., Moreau, P.A., Miller, A.N., Molia, A., Morte, A., Navarro-Ródenas, A., Nebesářová, J., Nigrone, E., Nuthan, B.R., Oberlies, N.H., Pepori, A.L., Rämä, T., Rapley, D., Reschke, K., Robicheau, B.M., Roets, F., Roux, J., Saavedra, M., Sakolrak, B., Santini, A., Ševčíková, H., Singh, P.N., Singh, S.K., Somrithipol, S., Spetik, M., Sridhar, K.R., Starink-Willemse, M., Taylor, V.A., van, Iperen, A.L., Vauras, J., Walker, A.K., Wingfield, B.D., Yarden, O., Cooke, A.W., Manners, A.G., Pegg, K.G. & Groenewald, J.Z. (2022) Fungal Planet description sheets: 1383–1435. Persoonia 48: 261–371. https://doi.org/10.3767/persoonia.2022.48.08
  9. Dong, W., Wang, B., Hyde, K.D., McKenzie, E.H.C., Raja, H.A., Tanaka, K., Abdel-Wahab, M.A., Abdel-Aziz, F.A., Doilom, M. & Phookamsak, R., Hongsanan, S., Wanasinghe, D.N., Yu, X.D., Wang, G.N., Yang, H., Yang, J., Thambugala, K.M., Tian, Q., Luo, Z.L., Yang, J.B., Miller, A.N., Fournier, J., Boonmee, S., Hu, D.M., Nalumpang, S. & Zhang, H. (2020) Freshwater Dothideomycetes. Fungal Diversity 105: 319–575. https://doi.org/10.1007/s13225-020-00463-5
  10. Duarte, S., Fernandes, I., Nogueira, M.J., Cássio, F. & Pascoal, C. (2013) Temperature alters interspecific relationships among aquatic fungi. Fungal Ecology 6: 187–191. https://doi.org/10.1016/j.funeco.2013.02.001
  11. Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.
  12. Hernández-Restrepo, M., Schumacher, R.K., Wingfield, M.J., Ahmad, I., Cai, L., Duong, T.A., Edwards, J., Gené, J., Groenewald, J.Z., Jabeen, S., Khalid, A.N., Lombard, L., Madrid, H., Marin-Felix, Y., Marincowitz, S., Miller, A.N., Rajeshkumar, K.-C., Rashid, A., Sarwar, S., Stchigel, A.M., Taylor, P.W.J., Zhou, N. & Crous, P.W. (2016) Fungal Systematics and Evolution: FUSE 2. Sydowia 68: 193–230. https://doi.org/10.12905/0380.sydowia68-2016-0193
  13. Hyde, K.D., Dong, Y., Phookamsak, R., Jeewon, R., Bhat, D.J., Jones, E.B.G., Liu, N.G., Abeywickrama, P.D., Mapook, A., Wei, D., Perera, R.H., Manawasinghe, I.S., Pem, D., Bundhun, D., Karunarathna, A., Ekanayaka, A.H., Bao, D.F., Li, J., Samarakoon, M.C., Chaiwan, N., Lin, C.G., Phutthacharoen, K., Zhang, S.N., Senanayake, I.C., Goonasekara, I.D., Thambugala, K.M., Phukhamsakda, C., Tennakoon, D.S., Jiang, H.B., Yang, J., Zeng, M., Huanraluek, N., Liu, J.K., Wijesinghe, S.N., Tian, Q., Tibpromma, S., Brahmanage, R.S., Boonmee, S., Huang, S.K., Thiyagaraja, V., Lu, Y.Z., Jayawardena, R.S., Dong, W., Yang, E.F., Singh, S.K., Singh, S.M., Rana, S., Lad, S.S., Anand, G., Devadatha, B., Niranjan, M., Sarma, V.V., Liimatainen, K., Aguirre-Hudson, B., Niskanen, T., Overall, A., Alvarenga, R.L.M., Gibertoni, T.B., Pfiegler, W.P., Horváth, E., Imre, A., Alves, A.L., Santos, A.C.S., Tiago, P.V., Bulgakov, T.S., Wanasinghe, D.N., Bahkali, A.H., Doilom, M., Elgorban, A.M., Maharachchikumbura, S.S.N., Rajeshkumar, K.C., Haelewaters, D., Mortimer, P.E., Zhao, Q., Lumyong, S., Xu, J. & Sheng, J. (2020) Fungal diversity notes1151–1276: taxonomic and phylogenetic contributions on genera and species of fungal taxa. Fungal Diversity 100: 5–277. https://doi.org/10.1007/s13225-020-00439-5
  14. Hyde, K.D., Tennakoon, D.S., Jeewon, R.J., Bhat, D.J., Maharachchikumbura, S.S., Rossi, W., Leonardi, M., Lee,H.B., Xu, J. & Doilom, M. (2019) Fungal diversity notes 1036–1150: taxonomic and phylogenetic contributions on genera and species of fungal taxa. Fungal Diversity 96: 1–242. https://doi.org/10.1007/s13225-019-00429-2
  15. Hyde, K.D., Fryar, S., Tian, Q., Bahkali, A.H. & Xu, J.C. (2016) Lignicolous freshwater fungi along a northsouth latitudinal gradient in the Asian/Australian region; can we predict the impact of global warming on biodiversity and function? Fungal Ecology 19: 190–200. https://doi.org/10.1016/j.funeco.2015.07.002
  16. Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30: 772–780. https://doi.org/10.1093/molbev/mst010
  17. Klaubauf, S., Tharreau, D., Fournier, E., Groenewald, J.Z., Crous, P.W., de Vries, R.P. & Lebrun, M.-H. (2014) Resolving the polyphyletic nature of Pyricularia (Pyriculariaceae). Studies in Mycology 79: 85–120. https://doi.org/10.1016/j.simyco.2014.09.004
  18. Koske, R.E. & Duncan, I.W. (1974) Temperature effects on growth, sporulation, and germination of some “aquatic” hyphomycetes.Canadian Journal of Botany 52: 1387–1391. https://doi.org/10.1139/b74-180
  19. Liu, J.K., Phookamsak, R., Doilom, M., Wikee, S., Li, Y.M., Ariyawansha, H., Boonmee, S., Chomnunti, P., Dai, D.Q., Bhat, J.D., Romero, A.I., Zhang, W.Y., Monkai, J., Jones, E.B.G., Chukeatirote, E., KoKo, T.W., Zhao, Y.C., Wang, Y. & Hyed, K.D. (2012) Towards a natural classification of Botryosphaeriales. Fungal Diversity 57: 149–210. https://doi.org/10.1007/s13225-012-0207-4
  20. Li, L.L., Shen, H.W., Bao, D.F., Lu, Y.Z., Su, H.Y. & Luo, Z.L. (2022) New species, Parahelicomyces yunnanensis sp. nov. and Tubeufia nigroseptum sp. nov. from freshwater habitats in Yunnan, China. Phytotaxa 530 (1): 21–37. https://doi.org/10.11646/phytotaxa.530.1.2
  21. Luan, S., Shen, H.W., Bao, D.F., Luo, Z.L. & Li, Y.X. (2023) Morphology and multi-gene phylogeny reveal a novel Torula (Pleosporales, Torulaceae) species from the plateau lakes in Yunnan, China. Biodiversity Data Journal 11: e109477–e109477. https://doi.org/10.3897/BDJ.11.e109477
  22. Luo J., Yin J., Cai L., Zhang K.Q. & Hyde K.D. (2004) Freshwater fungi in Lake Dianchi, a heavily polluted lake in Yunnan, China. Fungal Diversity 16: 93–112.
  23. Luo, Z.L., Hyde, K.D., Liu, J.K., Maharachchikumbura, S.S.N., Jeewon, R., Bao, D.F., Bhat, D.J., Lin, C.G., Li, W.L., Yang, J., Liu, N.G., Lu, Y.Z., Jayawardena, R.S., Li, J.F. & Su, H.Y. (2019) Freshwater Sordariomycetes. Fungal Diversity. 99: 451–660. https://doi.org/10.1007/s13225-019-00438-1
  24. Luo, Z.L., Hyde, KD, Liu, J.K., Bhat, D.J., Bao, D.F., Li, W.L. & Su, H.Y. (2018) Lignicolous freshwater fungi from China II: Novel Distoseptispora (Distoseptisporaceae) species from northwestern Yunnan Province and a suggested unified method for studying lignicolous freshwater fungi. Mycosphere 9: 444–461. https://doi.org/10.5943/mycosphere/9/3/2
  25. Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES science gateway for inference of large phylogenetic trees. In: Proceedings of the 2010 gateway Computing Environments Workshop (gCE), November 2010. New Orleans, Louisiana, pp. 1–8. https://doi.org/10.1109/GCE.2010.5676129
  26. Nylander, J.A.A. (2004) MrModeltest v.2 Program Distributed by the Author. Uppsala University.
  27. Rannala, B. & Yang, Z. (1996) Probability distribution of molecular evolutionary trees: a new method of phylogenetic inference. Journal Molecular Evolution 43: 304–311. https://doi.org/10.1007/BF02338839
  28. Réblová, M., Fournier, J. & Štěpánek, V. (2016). Two new lineages of aquatic ascomycetes: Atractospora gen. nov. and Rubellisphaeria gen. et sp. nov., and a sexual morph of Myrmecridium montsegurinum sp. nov. Mycological Progress 15: 1–18. https://doi.org/10.1007/s11557-016-1166-z
  29. Re´blova´, M. (2007) Barbatosphaeria gen. et comb. nov., a new genus for Calosphaeria barbirostris. Mycologia 99: 723–732. https://doi.org/10.1080/15572536.2007.11832536
  30. Ronquist, F., Teslenko, M., Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: Efficient bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61: 539–542. https://doi.org/10.1093/sysbio/sys029
  31. Senanayake, I.C., Rathnayaka, A.R., Marasinghe, D.S., Calabon, M.S., Gentekaki, E., Lee, H.B., Hurdeal, V.G., Pem, D., Dissanayake, L.S., Wanasinghe, D.N., Bundhun, D., Nguyen, T.T., Goonasekara, I.D., Abeywickrama, P.D., Bhunjun, C.S., Jayawardena, R.S., Wanasinghe, D.N., Jeewon, R., Bhat, D.J. & Xiang, M.M. (2020) Morphological approaches in studying fungi: collection, examination, isolation, sporulation and preservation. Mycosphere 11: 2678–2754. https://doi.org/10.5943/mycosphere/11/1/20
  32. Shen, H.W., Bao, D.F., Bhat, D.J., Su, H.Y. & Luo, Z.L. (2022) Lignicolous freshwater fungi in Yunnan Province, China: an overview. Mycology 13: 119–132. https://doi.org/10.1080/21501203.2022.2058638
  33. Stamatakis, A., Hoover, P. & Rougemont, J. (2008) A rapid bootstrap algorithm for the RAxML web-servers. Systematic Biology 75: 758–771. https://doi.org/10.1080/10635150802429642
  34. Stamatakis, A. (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and omixed models. Bioinformatics 22: 2688–2690. https://doi.org/10.1093/bioinformatics/btl446
  35. Su, H.Y., Hyde, K.D., Maharachchikumbura, S.S.N., Ariyawansa, H.A., Luo, Z.L., Promputtha, I., Tian, Q., Lin, C.G., Shang, Q.J., Zhao, Y.C., Chai, H.M., Liu, X.Y., Bahkali, A.H., Bhat, D.J., McKenzie, E.H.C. & Zhou, D.Q. (2016) The families Distoseptisporaceae fam. nov., Kirschsteiniotheliaceae, Sporidesmiaceae and Torulaceae, with new species from freshwater in Yunnan Province, China. Fungal Diversity 80: 375–409. https://doi.org/10.1007/s13225-016-0362-0
  36. Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172 (8): 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
  37. Vijaykrishna, D., Jeewon, R. & Hyde, K.D. (2006) Molecular taxonomy, origins and evolution of freshwater ascomycetes. Fungal Diversity 23: 367–406.
  38. Wanasinghe, D.N., Jones, E.B.G., Camporesi, E., Mortimer, P.E., Xu, J., Bahkali, A.H. & Hyde, K.D. (2015) The genus Murispora. Cryptogamie Mycologie 36: 419–448. https://doi.org/10.7872/crym/v36.iss4.2015.419
  39. Wang, G.N., Yu, X.D., Dong, W., Bhat, D.J., Boonmee, S., Zhang, D. & Zhang, H. (2019) Freshwater hyphomycetes in Eurotiomycetes: a new species of Minimelanolocus and a new collection of Thysanorea papuana (Herpotrichiellaceae). Mycological Progress 18: 511–522. https://doi.org/10.1007/s11557-019-01473-7
  40. White, T.J., Bruns, T., Lee, S. & Taylor, J.W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols: A Guide to Methods and Applications 18: 315–322. https://doi.org/10.1016/b978-0-12-372180-8.50042-1
  41. Xie, N., Phookamsak, R., Jiang, H.B., Zeng, Y.J., Zhang, H., Xu, F., Lumyong, S., Xu, J.C. & Hongsanan, S. (2022) Morpho-molecular characterization of five novel taxa in Parabambusicolaceae (Massarineae, Pleosporales) from Yunnan, China. Journal of Fungi 8: 108. https://doi.org/10.3390/jof8020108
  42. Zare-Maivan, H. & Shearer, C.A. (1988) Extracellular enzyme production and cell wall degradation by freshwater lignicolous fungi. Mycologia 80: 365–375. https://doi.org/10.1080/00275514.1988.12025551.
  43. Zhang, H., Dong, W., Hyde, K.D., Maharachchikumbura, S.S.N., Hongsanan, S., Bhat, D.J., Al-Sadi, A.M. & Zhang, D. (2017) Towards a natural classification of Annulatascaceae-like taxa: introducing Atractosporales ord. nov. and six new families. Fungal Diversity 85: 75–110. https://doi.org/10.1007/s13225-017-0387-z
  44. Zhang, Z., Schwartz, S., Wagner, L. & Miller, W. (2000) A greedy algorithm for aligning DNA sequences. Journal of Computational Biology 7: 203–214. https://doi.org/10.1089/10665270050081478