Abstract
A new Asteraceae species, Ixeridium malingheense Z.Li & Q.Xu sp. nov., from Xingyi City, south-western Guizhou, China, is described and illustrated based on morphological and molecular analyses. Compared with the other species of the genus, the species is most similar to I. yunnanense in a small stature, linear-lanceolate stem leaves, and short phyllaries. However, it is easily distinguished from the latter by its smaller basal leaves 18–34 × 5–13 mm (vs. 10–25 × 5 mm), spatulate or long-spatulate (vs. elliptic, lanceolate, or oblanceolate), petioles ca. 30–55 mm (vs. 10 mm to absent), outer phyllaries 1-seriate (vs. 2-seriate, unequal), achenes smaller 2.0–3.0 × 0.5–1.0 mm (vs. 3.2 × 0.6 mm), and a flowering period of March to May (vs. June). Molecular phylogenetic analyses based on nuclear ITS sequence data determined the systematic position of I. malingheense in Ixeridium.
References
- Bailey, C.D. & Doyle, J.J. (1999) Potential Phylogenetic Utility of the Low-Copy Nuclear Gene pistillata in Dicotyledonous Plants: Comparison to nrDNA ITS and trnL intron in Sphaerocardamum and other Brassicaceae. Molecular Phylogenetics and Evolution 13 (1): 20–30. https://doi.org/10.1006/mpev.1999.0627
- Cassini, H. (1822) Lactucées, Lactuceae. In: Cuvier, F. (Ed.) Dictionnaire des Sciences Naturelles, ed. 2, vol. 25. Levrault, Strasbourg, pp. 59–89.
- Deng, T., Zhang, J.W., Zhu, X.X., Zhang, D.G., Nie, Z.L. & Sun, H. (2014) Youngia zhengyiana (Asteraceae, Crepidinae), a new species from south China, with notes on the systematics of Youngia inferred from morphology and nrITS phylogeny. Phytotaxa 170 (4): 259–268. https://doi.org/10.11646/phytotaxa.170.4.3
- Hall, T.A. (1999) BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98. https://doi.org/10.1021/bk-1999-0734.ch008
- IUCN (2022) Guidelines for Using the IUCN Red List Categories and Criteria, Version 15.1. Prepared by the Standards and Petitions Subcommittee. Available from: https://www.iucnredlist.org/resources/redlistguidelines (accessed 20 July 2022)
- Li, Z., Yin, G.S., Tang, M. & Li, W.P. (2017) Aster oliganthus (Asteraceae, Astereae), a new species from western Sichuan, China, based on morphological and molecular data. Phytotaxa 326 (1): 54–62. https://doi.org/10.11646/phytotaxa.326.1.4
- Li, Z., Xiong, Y.C., Liao, J.J., Xiao J.W. & Li, W.P. (2020) Aster huangpingensis (Asteraceae, Astereae), a new species from Guizhou, China. Phytotaxa 433 (3): 235–244. https://doi.org/10.11646/phytotaxa.433.3.5
- Liu, B., Zhang, M., Bussmann, W.R., Liu, H.M., Liu, Y.Y., Peng, Y.D., Zu, K.L., Zhao, Y.M., Liu, Z.B. & Yu, S.X. (2018) Species richness and conservation gap analysis of karst areas: A case study of vascular plants from Guizhou, China. Global Ecology and Conservation 16: e00460. https://doi.org/10.1016/j.gecco.2018.e00460
- Liu, Y., Chen, Y.S. & Yang, Q.E. (2013) Generic status, circumscription, and allopolyploid origin of Faberia (Asteraceae: Cichorieae) as revealed by ITS and chloroplast DNA sequence data. Taxon 62 (6): 1235–1247. https://doi.org/10.12705/626.14
- Lu, Y.F., Xu, Y.L., Xie, W.Y., Zhang, H.W., Zheng, Z.H., Cai, X. & Jin, X.F. (2021) Two new species and a new combination from Zhejiang, East China. Phytokeys 184: 111–126. https://doi.org/10.3897/phytokeys.184.73327
- Mandel, J.R., Dikow, R.B., Siniscalchi, C.M., Thapa, R., Watson, L.E. & Funk, V.A. (2019) A fully resolved backbone phylogeny reveals numerous dispersals and explosive diversifications throughout the history of Asteraceae. Proceedings of the National Academy of Sciences 116 (28): 14083–14088. https://doi.org/10.1073/pnas.1903871116
- Nakamura, K., Chung, K.F., Huang, C.J., Kono, Y., Kokubugata, G. & Peng, C.I. (2012) Extreme habitats that emerged in the Pleistocene triggered divergence of weedy Youngia (Asteraceae) in Taiwan. Molecular Phylogenetics and Evolution 63 (2): 486–499. https://doi.org/10.1016/j.ympev.2012.01.023
- Nakamura, K., Chung, S.W., Kono, Y., Ho, M.J., Hsu, T.C. & Peng, C.I. (2014) Ixeridium calcicola (Compositae), a new limestone endemic from Taiwan, with notes on its atypical basic chromosome number, phylogenetic affinities, and a limestone refugium hypothesis. PLOS One 9 (10): e109797. https://doi.org/10.1371/journal.pone.0109797
- Pak, J.H. & Kawano, S. (1992) Biosystematic studies on the genus Ixeris and its allied genera (Compositae—Lactuceae). IV. Taxonomic Treatments and Nomenclature. Memoirs of the Faculty of Science, Kyoto University. Series of Biology. New series 15 (1–2): 29–61. [https://hdl.handle.net/2433/258921]
- Peng, Y.L., Zhang, Y., Gao, X.F., Tong, L.J., Li, L., Li, R.Y., Zhu, Z.M. & Xian, J.R. (2014) A phylogenetic analysis and new delimitation of Crepidiastrum (Asteraceae, tribe Cichorieae). Phytotaxa 159 (4): 241–255. https://doi.org/10.11646/phytotaxa.159.4.1
- Ripma, L.A., Simpson, M.G. & Hasenstab-Lehman, K. (2014) Geneious! simplified genome skimming methods for phylogenetic systematic studies: a case study in Oreocarya (Boraginaceae). Applications in Plant Science 2 (12): e1400062. https://doi.org/10.3732/apps.1400062
- Shih, C. (1993) On the classification of the Ixeris group (Compositae) from China. Acta Phytotaxonomica Sinica 31 (6): 533–548. [https://www.jse.ac.cn/CN/Y1993/V31/I6/533]
- Shih, C. & Kilian, N. (2011) Ixeridium (Asteraceae). In: Wu, Z.Y., Raven, P.H. & Hong, D.Y. (Eds.) Flora of China, vol. 20–21. Science Press, Beijing & Missouri Botanical Garden Press, St. Louis, pp. 328–330. [http://www.iplant.cn/foc]
- Stamatakis, A. (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22 (21): 2688–2690. https://doi.org/10.1093/bioinformatics/btl446
- Standley, D.M. & Katoh, K. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30 (4): 772–780. https://doi.org/10.1093/molbev/mst010
- Tanaka, K. & Takahara, Y. (2013) Intraspecific phylogenetic relationships in Ixeridium dentatum (Compositae) complex based on genetic and morphological evidence. Plant Systematics and Evolution 299: 1493–1502. https://doi.org/10.1007/s00606-013-0812-z
- Tzvelev, N.N. (1964) Ixeridium (A.Gray) Tzvel. In: Bobrov, E.G. & Tzvelev, N.N. (Eds.) Flora of the USSR, vol. 29. Academy of Sciences of the USSR, Moscow, pp. 388–393. [In Russian]
- Xiang, C.Y., Gao, F., Jakovlić, I., Lei, H.P., Hu, Y., Zhang, H., Zou, H., Wang, G.T. & Zhang, D. (2023) Using PhyloSuite for molecular phylogeny and tree-based analyses. iMeta 2 (1): e87. https://doi.org/10.1002/imt2.87
- Yao, G., Liu, Z.C., Lang, Y.X., Liao, D.S., Xu, J.B., Luo, S. & Deng, C.Y. (2022) Glochidion malingheense (Phyllanthaceae), a new species from Guizhou, China. Phytotaxa 561 (1): 104–110. https://doi.org/10.11646/phytotaxa.561.1.10
- Yin, D.H., Huang, T.F., Lu, Z. & Duan, L.D. (2021) A new species and a new series of Elatostema (Urticaceae) from south-western China. Phytokeys 180: 65–71. https://doi.org/10.3897/phytokeys.180.65813
- Yuan, Q. & Yang, Q.E. (2009) Anemone xingyiensis (Ranunculaceae), a new species from Guizhou, China. Botanical Studies 50 (4): 493–498. [https://www.cabdirect.org/cabdirect/abstract/20093355735]
- Zhang, C., Huang, C.H., Liu, M., Hu, Y., Panero, J., Luebert, F., Gao, T. & Ma, H. (2021) Phylotranscriptomic insights into Asteraceae diversity, polyploidy, and morphological innovation. Journal of Integrative Plant Biology 63 (7): 1273–1293. https://doi.org/10.1111/jipb.13078
- Zhang, J.W., Kilian, N., Huang, J.H. & Sun, H. (2023) Ixeridium sagittarioides (Asteraceae—Cichorieae) revisited: range extension and molecular evidence for its systematic position in the Lactuca alliance. Phytokeys 230: 115–130. https://doi.org/10.3897/phytokeys.230.107733
- Zhang, Z.G. (1980) Karst types in China. GeoJournal 4 (6): 541–570. https://doi.org/10.1007/BF00214219
- Zhou, Y.L., Wen, F., Li, Q.Y., Wang, Q. & Bai, X.X. (2023) Primulina malingheensis (Gesneriaceae), a new species from a karst area in Guizhou Province, China. Annales Botanici Fennici 60 (1): 61–65. https://doi.org/10.5735/085.060.0109