Skip to main content Skip to main navigation menu Skip to site footer
Responsive image
Issue: Vol. 625 No. 3: 14 Nov. 2023
Type: Article
Published: 2023-11-14
DOI: 10.11646/phytotaxa.625.3.4
Page range: 280-288
Abstract views: 111
PDF downloaded: 6

Myxospora poaceicola sp. nov. (Stachybotryaceae, Hypocreales), a novel myrothecium-like fungus from Digitaria sanguinalis (Poaceae)

School of Life Science and Technology; Center for Informational Biology; University of Electronic Science and Technology of China; Chengdu 611731; P.R. China
School of Life Science and Technology; Center for Informational Biology; University of Electronic Science and Technology of China; Chengdu 611731; P.R. China
Department of Soil Science; College of Food and Agriculture Sciences; King Saud University; P.O. Box 145111; Riyadh 11362; Saudi Arabia
School of Life Science and Technology; Center for Informational Biology; University of Electronic Science and Technology of China; Chengdu 611731; P.R. China
1 new taxon myrothecium-like fungus phylogenetic analysis Sichuan Province Sordariomycetes Fungi

Abstract

Fungi belonging to the family Stachybotryaceae play crucial roles in ecosystem dynamics, particularly in decomposing plant materials and pathogens on plants and animals. In this study, we report the isolation of two hitherto unknown hyphomycetous and myrothecium-like fungi collected from two different Poaceae plants in Sichuan Province, China. Molecular analysis based on ITS, LSU, TEF1, RPB2, TUB2, and CAL sequences of newly isolated strains revealed that they represent a previously undescribed species within the genus Myxospora. Phylogenetic investigations employing both maximum-likelihood (ML), maximum parsimony (MP) and Bayesian inference (BI) methods indicated that the new species forms a sister clade with M. masonii and M. graminicola. Nevertheless, M. poaceicola is distinct from these closely related species within the genus by having longer synnemata and wider conidia. We formally designate it as Myxospora poaceicola sp. nov. With the addition of M. poaceicola, the genus Myxospora now comprises six species. This discovery sheds light on the diversity of Myxospora fungi and underscores the importance of further taxonomic exploration in this genus.

 

References

  1. Ben, H.-Y., Gao, W., Qu, H.-Y., Chai, A.-L., Shi, Y.-X., Xie, X.-W. & Li, B.-J. (2016) New host record of Myrothecium roridum causing leaf spot on Abutilon megapotamicum from China. Journal of Phytopathology 164: 563–566. https://doi.org/10.1111/jph.12439
  2. Capella-Gutiérrez, S., Silla-Martínez, J.M. & Gabaldón, T. (2009) trimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses. Bioinformatics 25 (15): 1972–1973. https://doi.org/10.1093/bioinformatics/btp348
  3. Carbone, I. & Kohn, L.M. (1999) A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91 (3): 553–556. https://doi.org/10.1080/00275514.1999.12061051
  4. Chen, Y., Tian, W., Guo, Y., Madrid, H. & Maharachchikumbura, S.S.N. (2022) Synhelminthosporium gen. et sp. nov. and two new species of Helminthosporium (Massarinaceae, Pleosporales) from Sichuan Province, China. Journal of Fungi 8 (7): 712. https://doi.org/10.3390/jof8070712
  5. Chen, Y., Su, P., Hyde, K.D. & Maharachchikumbura, S.S.N. (2023a) Phylogenomics and diversification of Sordariomycetes. Mycosphere 14: 414–451. https://doi 10.5943/mycosphere/14/1/5
  6. Chen, Y., Su, P., Stadler, M., Xiang, R., Hyde, K.D., Tian, W. & Maharachchikumbura, S.S.N. (2023b) Beyond observation: genomic traits and machine learning algorithms for predicting fungal lifestyles. Mycosphere 14 (1): 1530–1563. https://doi.org/10.5943/mycosphere/14/1/17
  7. Crous, P.W., Shivas, R.G., Quaedvlieg, W., van der Bank, M., Zhang, Y., Summerell, B.A., Guarro, J., Wingfield, M.J., Wood, A.R., Alfenas, A.C., Braun, U., Cano-Lira, J.F., García, D., Marin-Felix, Y., Alvarado, P., Andrade, J.P., Armengol, J., Assefa, A., den Breeÿen, A., Camele, I., Cheewangkoon, R., De Souza, J.T., Duong, T.A., Esteve-Raventós, F., Fournier, J., Frisullo, S., García-Jiménez, J., Gardiennet, A., Gené, J., Hernández-Restrepo, M., Hirooka, Y., Hospenthal, D.R., King, A., Lechat, C., Lombard, L., Mang, S.M., Marbach, P.A.S., Marincowitz, S., Marin-Felix, Y., Montaño-Mata, N.J., Moreno, G., Perez, C.A., Pérez Sierra, A.M., Robertson, J.L., Roux, J., Rubio, E., Schumacher, R.K., Stchigel, A.M., Sutton, D.A., Tan, Y.P., Thompson, E.H., Vanderlinde, E., Walker, A.K., Walker, D.M., Wickes, B.L., Wong, P.T.W. & Groenewald, J.Z. (2014) Fungal Planet description sheets: 214–280. Persoonia 32 (1): 184–306. https://doi.org/10.3767/003158514X682395
  8. Cubeta, M.A., Echandi, E., Abernethy, T. & Vilgalys, R. (1991) Characterization of anastomosis groups of binucleate Rhizoctonia species using restriction analysis of an amplified ribosomal RNA gene. Phytopathology 81 (11): 1395–1400. https://doi.org/10.1094/phyto-81-1395
  9. Drobotko, V. (1945) Stachybotryotoxicosis. A new disease of horses and humans. American Review of Soviet Medicine 2 (3): 238–242.
  10. Dyląg, M., Spychała, K., Zielinski, J., Łagowski, D. & Gnat, S. (2022) Update on Stachybotrys chartarum – black mold perceived as toxigenic and potentially pathogenic to humans. Biology 11 (3): 352. https://doi.org/10.3390/biology11030352
  11. Fujinawa, M.F., Pontes, N.C., do Vale, H.M.M., Santos, N.F. & Halfeld-Vieira, B.A. (2016) First report of Myrothecium roridum causing Myrothecium leaf spot on Begonia in Brazil. Plant Disease 100 (3): 655–655. https://doi.org/10.1094/pdis-09-15-1097-pdn
  12. Glass, N.L. & Donaldson, G.C. (1995) Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Applied and Environmental Microbiology 61 (4): 1323–1330. https://doi.org/10.1128/aem.61.4.1323-1330.1995
  13. Hoang, D.T., Vinh, L.S., Flouri, T., Stamatakis, A., von Haeseler, A. & Minh, B.Q. (2018) MPBoot: fast phylogenetic maximum parsimony tree inference and bootstrap approximation. BMC Evolutionary Biology 18 (1): 11. https://doi.org/10.1186/s12862-018-1131-3
  14. Hong, C.F., Tsai, S.F., Yeh, H.C. & Fan, M.C. (2013) First report of Myrothecium roridum causing Myrothecium leaf spot on Dieffenbachia picta ‘Camilla’ in Taiwan. Plant Disease 97 (9): 1253–1253. https://doi.org/10.1094/pdis-02-13-0177-pdn
  15. Huelsenbeck, J.P. & Ronquist, F. (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17 (8): 754–755. https://doi.org/10.1093/bioinformatics/17.8.754
  16. Hyde, K.D., Dong, Y., Phookamsak, R., Jeewon, R., Bhat, D.J., Jones, E.B.G., Liu, N.G., Abeywickrama, P.D., Mapook, A., Wei, D., Perera, R.H., Manawasinghe, I.S., Pem, D., Bundhun, D., Karunarathna, A., Ekanayaka, A.H., Bao, D.F., Li, J., Samarakoon, M.C., Chaiwan, N., Lin, C.-G., Phutthacharoen, K., Zhang, S.-N., Senanayake, I.C., Goonasekara, I.D., Thambugala, K.M., Phukhamsakda, C., Tennakoon, D.S., Jiang, H.-B., Yang, J., Zeng, M., Huanraluek, N., Liu, J.-K., Wijesinghe, S.N., Tian, Q., Tibpromma, S., Brahmanage, R.S., Boonmee, S., Huang, S.-K., Thiyagaraja, V., Lu, Y.-Z., Jayawardena, R.S., Dong, W., Yang, E.-F., Singh, S.K., Singh, S.M., Rana, S., Lad, S.S., Anand, G., Devadatha, B., Niranjan, M., Sarma, V.V., Liimatainen, K., Aguirre-Hudson, B., Niskanen, T., Overall, A., Alvarenga, R.L.M., Gibertoni, T.B., Pfliegler, W.P., Horváth, E., Imre, A., Alves, A.L., da Silva Santos, A.C., Tiago, P.V., Bulgakov, T.S., Wanasinghe, D.N., Bahkali, A.H., Doilom, M., Elgorban, A.M., Maharachchikumbura, S.S.N., Rajeshkumar, K.C., Haelewaters, D., Mortimer, P.E., Zhao, Q., Lumyong, S., Xu, J. & Sheng, J. (2020) Fungal diversity notes 1151–1276: taxonomic and phylogenetic contributions on genera and species of fungal taxa. Fungal Diversity 100 (1): 5–277. https://doi.org/10.1007/s13225-020-00439-5
  17. Kalyaanamoorthy, S., Minh, B.Q., Wong, T.K.F., von Haeseler, A. & Jermiin, L.S. (2017) ModelFinder: fast model selection for accurate phylogenetic estimates. Nature Methods 14 (6): 587–589. https://doi.org/10.1038/nmeth.4285
  18. Katoh, K., Misawa, K., Kuma, K.I. & Miyata, T. (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Research 30 (14): 3059–3066. https://doi.org/10.1093/nar/gkf436
  19. Liu, Y.J., Whelen, S. & Hall, B.D. (1999) Phylogenetic relationships among ascomycetes: evidence from an RNA polymerse II subunit. Molecular Biology and Evolution 16 (12): 1799–1808. https://doi.org/10.1093/oxfordjournals.molbev.a026092
  20. Lombard, L., Houbraken, J., Decock, C., Samson, R.A., Meijer, M., Réblová, M., Groenewald, J.Z. & Crous, P.W. (2016) Generic hyper-diversity in Stachybotriaceae. Persoonia 36 (1): 156–246. https://doi.org/10.3767/003158516X691582
  21. Minh, B.Q., Schmidt, H.A., Chernomor, O., Schrempf, D., Woodhams, M.D., von Haeseler, A. & Lanfear, R. (2020) IQ-TREE 2: new models and efficient methods for phylogenetic inference in the genomic era. Molecular Biology and Evolution 37 (5): 1530–1534. https://doi.org/10.1093/molbev/msaa015
  22. Nag Raj, T.R. (1995) What is Myrothecium prestonii? Mycotaxon 53: 295–310.
  23. O’Donnell, K., Kistler, H.C., Cigelnik, E. & Ploetz, R.C. (1998) Multiple evolutionary origins of the fungus causing Panama disease of banana: Concordant evidence from nuclear and mitochondrial gene genealogies. Proceedings of the National Academy of Sciences 95 (5): 2044–2049. https://doi.org/10.1073/pnas.95.5.2044
  24. Paradis, E. & Schliep, K. (2018) ape 5.0: an environment for modern phylogenetics and evolutionary analyses in R. Bioinformatics 35 (3): 526–528. https://doi.org/10.1093/bioinformatics/bty633
  25. Page, E.H. & Trout, D.B. (2001) The role of Stachybotrys mycotoxins in building-related illness. AIHAJ - American Industrial Hygiene Association 62 (5): 644–648. https://doi.org/10.1080/15298660108984664
  26. Quaedvlieg, W., Kema, G.H.J., Groenewald, J.Z., Verkley, G.J.M., Seifbarghi, S., Razavi, M., Gohari, A.M., Mehrabi, R. & Crous, P.W. (2011) Zymoseptoria gen. nov.: a new genus to accommodate Septoria-like species occurring on graminicolous hosts. Persoonia 26 (1): 57–69. https://doi.org/10.3767/003158511X571841
  27. Rao, V. and Sutton, B. (1975) Synnematous fungi. Kavaka 3: 21–28.
  28. Seifert, K., Morgan-Jones, G., Gams, W. & Kendrick, B. (2011) The genera of Hyphomycetes. CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands.
  29. Su, P., Lu, Z., Tian, W., Chen, Y. & Maharachchikumbura, S.S.N. (2023) Six additions to the genus Periconia (Dothideomycetes: Periconiaceae) from graminaceous plants in China. Journal of Fungi 9 (3): 300. https://doi.org/10.3390/jof9030300
  30. Tian, W., Su, P., Chen, Y. & Maharachchikumbura, S.S.N. (2023) Four new species of Torula (Torulaceae, Pleosporales) from Sichuan, China. Journal of Fungi 9 (2): 150. https://doi.org/10.3390/jof9020150
  31. Toju, H., Tanabe, A.S., Yamamoto, S. & Sato, H. (2012) High-coverage ITS primers for the DNA-based identification of Ascomycetes and Basidiomycetes in environmental samples. PLOS ONE 7 (7): e40863. https://doi.org/10.1371/journal.pone.0040863
  32. Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. 172 (8): 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
  33. Wanasinghe, D.N. & Maharachchikumbura, S.S.N. (2023) Exploring the diversity and systematics of Phaeosphaeriaceae: taxonomic novelties from ecologically diverse habitats and their phylogenetic resolution. Journal of Fungi 9 (8): 853. https://doi.org/10.3390/jof9080853
  34. Yan, K., Abbas, M., Meng, L., Cai, H., Peng, Z., Li, Q., El-Sappah, A.H., Yan, L. & Zhao, X. (2021) Analysis of the fungal diversity and community structure in Sichuan dark tea during pile-fermentation. Frontiers in Microbiology 12. https://doi.org/10.3389/fmicb.2021.706714
  35. Yu, G., Smith, D.K., Zhu, H., Guan, Y. & Lam, T.T.-Y. (2017) ggtree: an r package for visualization and annotation of phylogenetic trees with their covariates and other associated data. Methods in Ecology and Evolution 8 (1): 28–36. https://doi.org/10.1111/2041-210X.12628
  36. Zhou, W., Wei, Q., Feng, R., Liu, Y., Liang, H., Li, J. & Yan, K. (2021) Diversity and spatial distribution of endophytic fungi in Cinnamomum longepaniculatum of Yibin, China. Archives of Microbiology 203 (6): 3361–3372. https://doi.org/10.1007/s00203-021-02325-3