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Issue: Vol. 658 No. 1: 2 Jul. 2024
Type: Article
Published: 2024-07-02
DOI: 10.11646/phytotaxa.658.1.2
Page range: 50-80
Abstract views: 102
PDF downloaded: 1

Evidence on the origin of Narcissus ×weickertii (Amaryllidaceae), an endemic daffodil of the SW Iberian Peninsula

Departamento de Biología Vegetal y Ecología, Universidad de Sevilla. PO Box 1095, ES-41080 Sevilla, Spain.
Departamento de Ciencias Agrarias y del Medio Natural, Escuela Politécnica Superior de Huesca, Universidad de Zaragoza, Spain.
Departamento de Biología Vegetal y Ecología, Universidad de Sevilla. PO Box 1095, ES-41080 Sevilla, Spain.
Departamento de Genética, Escuela Técnica Superior de Ingenieros Agrónomos y de Montes, Universidad de Córdoba, Campus Rabanales, Córdoba, Spain.
Paraje Natural Marismas del Odiel, Consejería de Sostenibilidad, Medio Ambiente y Economía Azul Huelva, Spain.
CONAHCYT-Instituto de Ecología, Departamento de Ecología Evolutiva, Universidad Nacional Autónoma de México A.P. 70-275, 04510 México, CDMX.
Departamento de Biología Vegetal y Ecología, Universidad de Sevilla. PO Box 1095, ES-41080 Sevilla, Spain.
plant morphology taxonomy karyotype analysis genomic in situ analyses monoploid hybrid West Mediterranean Region Monocots

Abstract

The hypothesis regarding the origin of Narcissus ×weickertii through hybridization of its putative parent species, N. papyraceus and N. gaditanus, is investigated in populations from the southwestern of the Iberian Peninsula. Multivariate analyses of quantitative morphological characters in both vegetative and flower structures, coupled with a descriptive analysis of qualitative traits, reveal that N. ×weickertii displays an intermediate morphology between its putative parents. Several anatomical traits of the leaf and scape provide additional support for its hybrid nature. Karyotype analyses show that N. ×weickertii has a 2n=21 karyotype, which falls between those of the putative parent species, with several chromosomes showing an inability to pair. Fluorescence in situ hybridization procedures, encompassing both FISH (targeting NOR regions) and GISH (using total DNA from the putative parents), yield conclusive evidence regarding the hybrid nature of N. ×weickertii and confirm the hypothesis about the identity of their putative parent species. Furthermore, phylogenetic relationships suggest that N. gaditanus likely served as the maternal parent, while N. papyraceus acted as the paternal species, maintaining the hybrid species the style polymorphism of their parent species. The degree of viability observed in the N. ×weickertii pollen grains might indicate that it could bear some possibilities of sexual reproduction beyond the typical vegetative propagation of many described Narcissus hybrid species.

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