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Issue: Vol. 658 No. 1: 2 Jul. 2024
Type: Article
Published: 2024-07-02
DOI: 10.11646/phytotaxa.658.1.2
Page range: 50-80
Abstract views: 0
PDF downloaded: 0

Evidence on the origin of Narcissus ×weickertii (Amaryllidaceae), an endemic daffodil of the SW Iberian Peninsula

Departamento de Biología Vegetal y Ecología, Universidad de Sevilla. PO Box 1095, ES-41080 Sevilla, Spain.
Departamento de Ciencias Agrarias y del Medio Natural, Escuela Politécnica Superior de Huesca, Universidad de Zaragoza, Spain.
Departamento de Biología Vegetal y Ecología, Universidad de Sevilla. PO Box 1095, ES-41080 Sevilla, Spain.
Departamento de Genética, Escuela Técnica Superior de Ingenieros Agrónomos y de Montes, Universidad de Córdoba, Campus Rabanales, Córdoba, Spain.
Paraje Natural Marismas del Odiel, Consejería de Sostenibilidad, Medio Ambiente y Economía Azul Huelva, Spain.
CONAHCYT-Instituto de Ecología, Departamento de Ecología Evolutiva, Universidad Nacional Autónoma de México A.P. 70-275, 04510 México, CDMX.
Departamento de Biología Vegetal y Ecología, Universidad de Sevilla. PO Box 1095, ES-41080 Sevilla, Spain.
plant morphology taxonomy karyotype analysis genomic in situ analyses monoploid hybrid West Mediterranean Region Monocots

Abstract

The hypothesis regarding the origin of Narcissus ×weickertii through hybridization of its putative parent species, N. papyraceus and N. gaditanus, is investigated in populations from the southwestern of the Iberian Peninsula. Multivariate analyses of quantitative morphological characters in both vegetative and flower structures, coupled with a descriptive analysis of qualitative traits, reveal that N. ×weickertii displays an intermediate morphology between its putative parents. Several anatomical traits of the leaf and scape provide additional support for its hybrid nature. Karyotype analyses show that N. ×weickertii has a 2n=21 karyotype, which falls between those of the putative parent species, with several chromosomes showing an inability to pair. Fluorescence in situ hybridization procedures, encompassing both FISH (targeting NOR regions) and GISH (using total DNA from the putative parents), yield conclusive evidence regarding the hybrid nature of N. ×weickertii and confirm the hypothesis about the identity of their putative parent species. Furthermore, phylogenetic relationships suggest that N. gaditanus likely served as the maternal parent, while N. papyraceus acted as the paternal species, maintaining the hybrid species the style polymorphism of their parent species. The degree of viability observed in the N. ×weickertii pollen grains might indicate that it could bear some possibilities of sexual reproduction beyond the typical vegetative propagation of many described Narcissus hybrid species.

References

  1. Aedo, C. (2013) Narcissus. In: Talavera, S., Fernández Piedra, M.P., Rico, E., Crespo, M.B., Quintanar, A., Herrero, A. & Aedo, C. (Eds.) Flora Iberica 20. Real Jardín Botánico de Madrid, CSIC, Madrid, pp. 340–397. [http://www.floraiberica.es/floraiberica/texto/pdfs/20_184_05_Narcissus.pdf]
  2. Álvarez González, J.F., Castro Prigent, P., Gómez Murillo, P., Sánchez García, A. (2019) Dos nuevos híbridos de Narcissus L., (Amaryllidaceae) en la Península Ibérica. Folia Botanica Extremadurensis 13 (2): 33–38.
  3. Amarillo Vargas, J.M. & Sánchez-García, I. (2022) Nuevo híbrido de Narcissus L. (Amaryllidaceae) para la flora ibérica: Narcissus ×xereciensis Amarillo & I.Sánchez García, en el sur de España. Revista Sociedad Gaditana de Historia Natural 16: 1–3. ISSN: 1577-2578. e-ISSN: 2340-5759.
  4. Anthos (2014+) Sistema de información de las plantas de España. Real Jardín Botánico Madrid, CSIC-Fundación Biodiversidad, Madrid, Spain. Available at http://www.anthos.es/ (accessed: 5 September 2021).
  5. Arroyo, J. (2002) Narcissus (Amaryllidaceae), la evolución de los polimorfismos florales y la conservación más allá de las “listas rojas”. Revista Chilena de Historia Natural 75: 39–55. http://dx.doi.org/10.4067/S0716-078X2002000100005
  6. Arroyo, J. & Barrett, S.C.H. (2000) Discovery of distyly in Narcissus (Amaryllidaceae). American Journal of Botany 87: 748–751. https://doi.org/10.2307/2656861
  7. Arroyo, J., Barrett, S.C.H., Hidalgo, R. & Cole, W.W. (2002) Evolutionary maintenance of stigma-height dimorphism in Narcissus papyraceus (Amaryllidaceae). American Journal of Botany 89 (8): 1242–1249. https://doi.org/10.3732/ajb.89.8.1242
  8. Barra Lázaro, A., Díez Domínguez, R. & Ureña Plaza, F. (2016) Novedades en Narcissus L. (Amaryllidaceae). Flora Montiberica 63: 103–111. ISSN: 1138-5952.
  9. Barrett, S.C.H & Harder, L.D. (2005) The evolution of polymorphic sexual systems in daffodils (Narcissus). New Phytologist 165: 45–53. https://doi.org/10.1111/j.1469-8137.2004.01183.x
  10. Barrett, S.C.H., Lloyd, D.G. & Arroyo, J. (1996) Stylar polymorphisms and the evolution of heterostyly in Narcissus (Amaryllidaceae). In: Lloyd, G. et al. (Eds.) Floral Biology: 339–376. Chapman & Hall, New York. https://doi.org/10.1007/978-1-4613-1165-2_13
  11. Blanchard, J.W. (1990) Narcissus. A guide to wild daffodils. Alpine Garden Society, Surrey. 203 pp.
  12. Blanchard, J.W. (1998) Daffodil and Tulip Year Book. Royal Horticultural Society, London.
  13. Boissier, P.E. (1859) Diagnoses Plantarum Orientalium Novarum, ser. 2, no.4. B. Hermann, Lipsiae [Leipzig], 86 pp.
  14. Brandham, P.E. (1986) Evolution of polyploidy in cultivated Narcissus subgenus Narcissus. Genetica 68: 161–167. https://doi.org/10.1007/BF02424439
  15. Brandham, P.E. & Kirton, P.R. (1987) The chromosomes of species, hybrids and cultivars of Narcissus L. (Amaryllidaceae). Kew Bulletin 42: 65–102. https://doi.org/10.2307/4109898
  16. Bretagnolle, F. (2001) Pollen production and spontaneous polyploidization in diploid populations of Anthoxanthum alpinum. Biological Journal of the Linnean Society 72: 241–247. https://doi.org/10.1111/j.1095-8312.2001.tb01314.x
  17. Bretagnolle, F. & Thompson, J.D. (1995) Gametes with the somatic chromosome number: mechanisms of their formation and role in the evolution of autopolyploid plants. New Phytologist 129: 1–22. https://doi.org/10.1111/j.1469-8137.1995.tb03005.x
  18. Buitendijk, J.H. & Ramanna, M.S. (1996) Giemsa C-banded karyotypes of eight species of Alstroemeria L. and some of their hybrids. Annals of Botany 78: 449–457. https://doi.org/10.1006/anbo.1996.0141
  19. Cabrera, A., Martín, A. & Barro, F. (2002) In-situ comparative mapping (ISCM) of Glu-1 loci in Triticum and Hordeum. Chromosome Research 10: 49–54. https://doi.org/10.1023/A:1014270227360
  20. D’Amato, G. (2004) Karyotype and heterochromatin characterization in some species of Narcissus (Amaryllidaceae). Caryologia 57 (1): 99–105. https://doi.org/10.1080/00087114.2004.10589377
  21. Díaz Lifante, Z., Andrés Camacho, C., Viruel Sánchez, J. & Cabrera Caballero, A. (2009) The allopolyploid origin of Narcissus obsoletus (Amaryllidaceae): Identification of parental genomes by karyotype characterization and genomic in situ hybridization. Botanical Journal of the Linnaean Society 159: 477–498. https://doi.org/10.1111/j.1095-8339.2009.00951.x
  22. Dorda Alcaraz, E. (1983) Estudios cromosomáticos en el género Narcissus L. Fontqueria 4: 11–20.
  23. Dorda Alcaraz, E. & Fernández Casas, J. (1984a) Estudios morfológicos en el género Narcissus L. Sección de la hoja. Fontqueria 5: 15–22.
  24. Dorda Alcaraz, E. & Fernández Casas, J. (1984b) Estudios morfológicos en el género Narcissus L. Histología de hoja y escapo, II. Fontqueria 6: 7–18.
  25. Dorda Alcaraz, E. & Fernández Casas, J. (1989) Estudios anatómicos del género Narcissus L. Anatomía de hoja y escapo, III. Fontqueria 27: 103–162.
  26. Dorda Alcaraz, E. & Fernández Casas, J. (1994) Estudios morfológicos en el género Narcissus L. Anatomía de hoja y escapo, IV. Fontqueria 39: 69–150.
  27. Dulberger, R. (1964) Flower dimorphism and self-incompatibility in Narcissus tazetta L. Evolution 18: 361–363. https://doi.org/10.2307/2406347
  28. Durieu, M.C. (1847) Revue Botanique; Recueil Mensuel 2. P. Duchartre, Paris, 592 pp.
  29. Fernandes, A. (1937) Le problème de Narcissus tazetta L. I. Les formes à 22 chromosomes somatiques. Boletim da Sociedade Broteriana, Série 2 12: 159–219.
  30. Fernandes, A. (1939) Sur la caryo-systématique du groupe Jonquilla du genre Narcissus L. Boletim da Sociedade Broteriana, Série 2 13: 487–544.
  31. Fernandes, A. (1968) Keys to the identification of native and naturalized taxa of the genus Narcissus L. Daffodil Tulip Year Book 59: 37–66.
  32. Fernandes, A. (1969) Contribution to the knowledge of the biosystematics of some species of the genus Narcissus L. V Simposio de Flora Europaea. Sevilla. pp. 245–284.
  33. Fernandes, A. (1975) L’evolution chez le genre Narcissus L. Anales del Instituto Botánico Cavanilles 32: 843–872.
  34. Fernandes, A. (1990) Sur l’origine et le comportment des formes poliploides chez la section Bulbocodii DC. du genre Narcissus L. au Portugal. I. Les plantes des régions non éloignées du littoral occidental. Broteria Genetica 11 (86): 9–68.
  35. Fernandes, A. & Queirós, M. (1970) Sur quelques particularités d’une population triploide de Narcissus gaditanus Boiss. & Reut. Boletim da Sociedade Broteriana, Série 2 44: 55–66.
  36. Fernandes, A. & Queirós, M. (1971) Contribution à là connaissance cytotaxonomique des Spermatophyta du Portugal. II. Compositae. Boletim da Sociedade Broteriana, Série 2 45: 5–121.
  37. Fernández Casas, J. (1977) Recuentos cromosómicos en plantas vasculares españolas. Saussurea. 8: 35–55.
  38. Fernández Casas, J. (1996) Narcissorum notulae, II. Fontqueria 44: 253–262.
  39. Fernández Casas, J. (2002) Narcissus. In: Valdés, B., Rejdali, M., Achhal El Kadmiri, A., Jury, S.L. & Montserrat, J.M. (Eds.) Catalogue des plantes vasculaires du nord du Maroc, incluant des cle’s d’identification, vol. 2: 879–882. CSIC Biblioteca de Ciencias, Madrid. [https://bibdigital.rjb.csic.es]
  40. Fernández Casas, J. (2008) Narcissorum notulae, X. Fontqueria 55 (67): 547–558.
  41. Fernández Casas, J., García Guardia, C. & Pajarón, S. (1979) Números cromosómicos para la flora española, 91–92. Lagascalia 9: 118–119.
  42. Fernández Casas, J., Laínz, M. & Ruíz Rejón, M. (1973) Narcissus cuatrecasasii Fernández Casas, Laínz & Ruiz Rejón Narcissus cuatrecasasii stat. et nom. nov. Cuad. Cienc. Biol. (Granada) 2 (1): 3–5.
  43. Fernández Casas, J., Molero Briones, J. & Pons Sorolla, A. (1980) Números cromosomáticos de plantas occidentales, 70–2. Anales Jardín Botánico Madrid 37 (1): 203–204.
  44. Font Quer, P. (1927) Notas sobre la flora gaditana. Boletín de la Sociedad Española de Historia Natural 27: 39–46.
  45. Gómez-Murillo, P & Arellano-Martín, I. (2020) Narcissus × splendidus (Amaryllidaceae), una nueva especie híbrida en Andalucía. Flora Montiberica 77: 112–113. [ISSN 1138-5952 – eISSN 1988-799X]
  46. Gómez-Murillo, P., Arellano-Martín, I., Álvarez González, J.F., Sánchez García, A. & Castro Prigent, P. (2020) Revisión del género Narcissus L. (Amaryllidaceae) en Andalucía. Folia Botanica Extremadurensis 14: 25–38. [ISSN 1179-3155 – eISSN 1179-3163]
  47. Gómez-Murillo, P. & Hervás-Serrano, J.L. (2019) Narcissus vilchezii, una nueva especie (Amaryllidaceae) en Andalucía (España). Flora Montiberica 75: 57–58. [ISSN 1138-5952 – eISSN 1988-799X]
  48. Gouan, A. (1773) Illustrationes et Observationes Botanicae, ad specierum historiam facientes. Apud Orell, Gessner, Fuesslin & Socios, Tiguri, 83 pp.
  49. Goulet, B.E., Roda, F. & Hopkins, R. (2017) Hybridization in plants: old ideas, new techniques. Plant Physiology 173: 65–78. https://doi.org/10.1104/pp.16.01340
  50. Greilhuber, J. & Speta, F. (1989) A Giemsa C-banding and DNA content study in Scilla ciclica and S. morrisii, two little known sibling species of the S. siberica alliance (Hyacynthaceae). Plant Systematic and Evolution 165: 71–83. https://doi.org/10.1007/BF00936036
  51. Hanks, G.R. (2002) Narcissus and Daffodil: The Genus Narcissus. CRC Press, London, 452 pp. https://doi.org/10.1201/9780203219355
  52. Huelsenbeck, J.P. & Ronquist, F. (2001) MRBAYES: Bayesian inference of phylogeny. Bioinformatics 17: 754–755.
  53. Kálmán, K., Medvegy, A. & Mihalik, E. (2004) Pattern of the floral variation in the hybrid zone of two distylous Primula species. Flora-Morphology, Distribution, Functional Ecology of Plants 199: 218–227. https://doi.org/10.1078/0367-2530-00149
  54. Ker Gawler, J.B. (1806) Narcissus papyraceus (α). Italian or paper-white Narcissus. Curtis’s Botanical Magazine 24: 947–949.
  55. Levan, A.K., Fredga, K. & Sandberg, A.A. (1965) Nomenclature for centromeric position on chromosomes. Hereditas 52: 201–220.
  56. Linnaeus, C. (1753) Species Plantarum 1. Holmiae: Laurentii Salvii. 560 pp. https://doi.org/10.5962/bhl.title.37656
  57. López Tirado, J. (2018) Narcissus ×munozii-alvarezii (Amaryllidaceae): A new hybrid from southern Spain. Phyotaxa 364 (3): 267–274. https://doi.org/10.1007/s00606-008-0015-1
  58. López Tirado, J. (2019) A natural laboratory in southern Spain: new hybrids of wild daffodils (Narcissus, Amaryllidaceae). Phytotaxa 394: 161–170. https://doi.org/10.11646/phytotaxa.394.2.4
  59. López Tirado, J. (2020) Taxonomic novelties in Narcissus L. (Amaryllidaceae): Narcissus ×villanovensis nothosp. nov. and N. ×koshinomurae nothosubsp. sanchezii comb. nov. Nordic Journal of Botany 38 (11): 1–6. https://doi.org/10.1111/njb.02915
  60. Löve, Á. & Löve, D. (1975) Plant chromosomes. Vaduz: J. Cramer, Beaverton, Oregon. 184 pp. https://doi.org/10.1111/j.1601-5223.1964.tb01953.x
  61. Maddison, W.P. & Maddison, D.R. (2023) Mesquite: a modular system for evolutionary analysis. Version 3.81. [http://www.mesquiteproject.org]
  62. Maire, R. (1921) Bulletin de la Société d’Histoire Naturelle de l’Afrique du Nord. Algiers, 12. 208 pp. Société d’histoire naturelle de l’Afrique du nord (imprimerie E. Mazel).
  63. Maire, R. (1959) Flore de l’Afrique du Nord, 6. Paul Lechevalier, Paris. 397 pp.
  64. Marques, I., Aguilar, J.F., Martins-Loução, M.A., Moharrek, F. & Nieto Feliner, G. (2017) A three-genome five-gene comprehensive phylogeny of the bulbous genus Narcissus (Amaryllidaceae) challenges current classifications and reveals multiple hybridization events. Taxon 66: 832–854. https://doi.org/10.12705/664.3
  65. Marques, I., Jürgens, A., Fuertes Aguilar, J. & Nieto Feliner, G. (2016) Convergent recruitment of new pollinators is triggered by independent hybridization events in Narcissus. New Phytologist 210 (2). https://doi.org/10.1111/nph.13805
  66. Marques, I., Loureiro, J., Draper, D., Castro, M. & Castro, S. (2018) How much do we know about the frequency of hybridisation and polyploidy in the Mediterranean region?. Plant Biology 20: 21–37. https://doi.org/10.1111/plb.12639
  67. Marques, I., Nieto Feliner, G., Draper, D., Martins-Loução, A. & Fuertes Aguilar, J. (2010) Unraveling cryptic reticulate relationships and the origin of orphan hybrid disjunct populations in Narcissus. Evolution 64: 2353–2368. https://doi.org/10.1111/j.1558-5646.2010.00983.x
  68. Marques, I., Nieto Feliner, G., Martins-Loução, A. & Fuertes Aguilar, J. (2012) Genome size and base composition variation in natural and experimental Narcissus (Amaryllidaceae) hybrids. Annals of Botany 109 (1): 257–264. https://doi.org/10.1093/aob/mcr282
  69. Mathew, B. (2002) Classification of the genus Narcissus. In: Gordon, R.H. (Ed.) Narcissus and Daffodil. Kirton: Horticulture Research International, pp. 30–52.
  70. Médail, F. & Quézel, P. (1997) Hot-Spots analysis for conservation of plant biodiversity in the Mediterranean Basin. Annals of the Missouri Botanical Garden 84 (1): 112–127. https://doi.org/10.2307/2399957
  71. Myers, N., Mittermeier, R.A., Mittermeier, C.G., Fonseca, G.A.B. & Kent, J. (2000) Biodiversity hotspots for conservation priorities. Nature 403: 853–858. https://doi.org/10.1038/35002501
  72. Moreno Guerrero, M., Loza Fernández, J., Fernández Peralta, A.M. & González Aguilera, J.J. (1981) Números cromosomáticos de plantas occidentales, 134–137. Anales Jardín Botánico Madrid 38 (1): 285–286.
  73. Parlatore, F. (1858) Flora Italiana 3 (1). Firenze, Tip. Le Monnier. 690 pp.
  74. Pedro Gomes, J. (1947) Uma nova espécie de Narcissus L. Boletim da Sociedade Broteriana sér. 2, 21: 60–61. Available at: https://www.yumpu.com/en/document/read/13575875 (accessed 1 Jun. 2024)
  75. Pérez‐Barrales, R., Vargas, P. & Arroyo, J. (2006) New evidence for the Darwinian hypothesis of heterostyly: breeding systems and pollinators in Narcissus sect. Apodanthi. New Phytologist 171 (3): 553–567. https://doi.org/10.1111/j.1469-8137.2006.01819.x
  76. Posada, D. (2008) jModelTest: phylogenetic model averaging. Molecular Biology and Evolution 25 (7): 1253–1256. https://doi.org/10.1093/molbev/msn083
  77. Ramsey, J. & Schemske, D. (1998) Pathways, mechanisms, and rates of polyploidy formation in flowering plants. Annual Review of Ecology and Systematics 29: 467–501. https://doi.org/10.1146/annurev.ecolsys.29.1.467
  78. Ran, Y., Murray, B.G. & Hammett, K.R.W. (1999) Karyotype analysis of the genus Clivia by Giemsa and flourochrome banding and in situ hybridation. Euphytica 106: 139–147. https://doi.org/10.1023/A:1003572705445
  79. Redouté, P.J. (1816) Les Liliacées 8. Imprimerie de Didot Jeune. Paris. 126 pp., t. 486. https://doi.org/10.5962/bhl.title.537
  80. Ribeiro, P., Paiva, J. & Freitas, H. (2007) Narcissus ×caramulensis (Amaryllidaceae), a new hybrid for the Portuguese flora. Anales del jardín Botánico de Madrid 64 (1): 43–46. https://doi.org/10.3989/ajbm.2007.v64.i1.50
  81. Romero, A.T., Sánchez Castillo, P.M. & Ruíz Rejón, M. (1983) Sobre cariología, morfología y corología de Narcissus tortifolius Fernández Casas. Fontqueria 4: 7–10.
  82. Romero Zarco, C. (1986) A new method for estimating karyotype asymmetry. Taxon 35: 526–530. https://doi.org/10.2307/1221906
  83. Sánchez Gullón, E. & Castro Prigent, P. (2011) Narcissus ×weickertii nothosp. nov. para Huelva (Andalucía occidental, España). Lagascalia 31: 203–206.
  84. Sanders, T. (2014) Hybrids of different diploid narcissus species can be fertile by generating unreduced pollen (October 2014). Available from: https://dafflibrary.org (accessed 1 Jun. 2024)
  85. Santos-Gallly, R., González-Voyer, A. & Arroyo, J. (2013) Deconstructing heterostyly: The evolutionary role of incompatibility system, pollinators, and floral architecture. Evolution 67 (7): 2072–2082. https://doi.org/10.1111/evo.12087
  86. Santos-Gally, R., Pérez-Barrales, R., Simón-Porcar, V.I. & Arroyo, J. (2011) The role of short-tongued insects in floral variation across the range of a style-dimorphic plant. Annals of Botany 111: 317–328. https://doi.org/10.1093/aob/mcs261
  87. Santos-Gally, R., Vargas, P. & Arroyo, J. (2012) Insights into Neogene Mediterranean biogeography based on phylogenetic relationships of mountain and lowland lineages of Narcissus (Amaryllidaceae). Journal of Biogeography 39 (4): 782–798. https://doi.org/10.1111/j.1365-2699.2011.02526.x
  88. Scrugli, A. (1973) Numeri cromosomici per la flora italiana: 162–166. Informatore botánico italiano 5: 264–270.
  89. Scrugli, A. (1977) Numeri cromosomici per la flora italiana: 331–347. Informatore botánico italiano 9: 116–125.
  90. Schultes, J.H. fil. (1830) Systema vegetabilium. Editio nova, speciebus inde ab editione XVbis. Detectis aucta et locupletata, 7 (2). Stuttgardtiae. 1061 pp. [https://bibdigital.rjb.csic.es/records/item/10314-systema-vegetabilium?offset=38]
  91. Simón-Porcar, V.I., Picó Mercader, F.J. Arroyo, J. (2015) Range-wide population genetics and variation in floral morph ratio in style-dimorphic Narcissus papyraceus (Amaryllidaceae). American Journal of Botany 102: 449–456. https://doi.org/10.3732/ajb.1400209
  92. Spach, É. (1846) Histoire Naturelle des Végétaux. Phanérogames, 12. Librairie Encyclopédique de Roret, Paris, 458 pp.
  93. Stebbins, G.L. (1938) Cytological characteristics associated with the different growth habits in the dicotyledons. American Journal of Botany 25: 189–198. https://doi.org/10.2307/2436589
  94. Stern, F.C.S. (1950) Daffodil Tulip Year Book 25. Royal Horticultural Society, London.
  95. Steudel, E.G. (1841) Nomenclator botanicus 2nd ed. Stuttgardtiae & Tubingae. 809 pp. [https://www.biodiversitylibrary.org/bibliography/655]
  96. Snow, R. (1963) Alcoholic hydrochloric acid carmine as a stain for chromosomes in squash preparations. Stain Technology 38: 9–14. https://doi.org/10.3109/10520296309061161
  97. Suzzi, S., Vosa, C.F. & Bassi, P. (1997) Repetitive DNA as marker for the study of male gametogenesis in higher plants. I. Analysis in some species of Tradescantia and Narcissus. Caryologia 50 (1): 67–76. https://doi.org/10.1080/00087114.1997.10797385
  98. Thompson, J.D. (2020) Plant Evolution in the Mediterranean: Insights for conservation. 2nd ed. Oxford University Press, Oxford, 464 pp. https://doi.org/10.1093/oso/9780198835141.001.0001
  99. Tjio, J.H. & Levan, A. (1950) The use of oxyquinoline in chromosome analysis. Anales Estación Experimental Aula Dei 2: 21–64. [https://digital.csic.es/bitstream/10261/33606/1/AnalesEEADv2n1_1950.PDF]
  100. Valdés Bermejo, E. & Gómez García, J. (1976) Notas cariosistemáticas sobre flora española. Acta Bot. Malacitana 2: 39–50. https://doi.org/10.24310/Actabotanicaabmabm.v2i.9704
  101. Valdés Bermejo, E. (1980) Números cromosomáticos de plantas occidentales, 1-34, Anales del Jardín Botánico de Madrid. 36: 373–389. [https://rjb.csic.es//jardinbotanico/ficheros/documentos/pdf/anales/1979/Anales_36(1)_371_405.pdf]
  102. Valdés, B. (1987) Narcissus L. In: B. Valdés, S. Talavera & Galiano, E. (Eds.) Flora vascular de Andalucía occidental 3. Ketres, Barcelona, pp. 463–474.
  103. Webb, D.A. (1978) Taxonomic notes on Narcissus L. Botanical Journal of the Linnean Society 76: 298–307. https://doi.org/10.1111/j.1095-8339.1978.tb01817.x
  104. Webb, D.A. (1980) Narcissus L. In: Tutin, T.G., Heywood, V.H., Burges, N.A., Moore, D.M., Valentine, D.H., Walters, S.M. & Webb, D.A. (Eds.) Flora Europaea 3. Cambridge University Press, Cambridge, pp. 78–84.
  105. Wedderburn, F.M. & Richards, A.J. (1992) “Secondary homostyly in Primula L.; evidence for the model of the ‘S’supergene.” New Phytologist 121 (4): 649–655. https://doi.org/10.1111/j.1469-8137.1992.tb01136.x
  106. Wu, H., Ramanna, M.S., Arens, P. & van Tuyl, J.M. (2011) Genome constitution of Narcissus variety, ‘Tête-à-Tête’, analysed through GISH and NBS profiling. Euphytica 181: 285–292. https://doi.org/10.1007/s10681-011-0438-6
  107. Zonneveld, B.J.M. (2008) The systematic value of nuclear DNA content for all species of Narcissus L. (Amaryllidaceae). Plant Systematic and Evolution 275: 109–132. https://doi.org/10.1007/s00606-008-0015-1