Abstract
Urnula is a prominent and older genus within the family Sarcosomataceae. Its members are widely distributed and have very similar features in the field. This study proposes two novel Urnula species collected from Yunnan Province, China. Detailed descriptions, illustrations, and phylogenetic analyses are provided. Urnula ailaoshanensis grows on rotten wood surrounded by moss and is characterized by its brighter hymenial surface, wider asci, and longer ascospores. Urnula subcrateria was collected on the soil humus layer of a subtropical forest with many fallen leaves, mosses, and lichens. It is morphologically and molecularly related to Urnula craterium (Schwein.) Fr., but can easily be distinguished by its shorter stipe and smaller asci and ascospores.
References
- Blanchette, R.A., Held, B.W., Hellmann, L., Millman, L. & Büntgen, U. (2016) Arctic driftwood reveals unexpectedly rich fungal diversity. Fungal Ecology 23: 58–65. https://doi.org/10.1016/j.funeco.2016.06.001
- Boudier, J.L.É. & Torrend, C. (1911) Discomycètes nouveaux de Portugal. Bulletin Trimestriel de la Société Mycologique de France 27: 127–136.
- Brunelli, F. [1998 (1997)] Urnula brachysperma ad int. Bollettino del Gruppo Micologico ‘G. Bresadola’ 40: 119–122.
- Carbone, M. & Agnello, C. (2013a) Notes on Urnula campylospora (Pezizales). Ascomycete.org 5: 25–32. https://doi.org/10.25664/art-0077
- Carbone, M. & Agnello, C. (2013b) Notes on Urnula hiemalis Nannf. Ascomycete.org 5: 53–61. https://doi.org/10.25664/art-0081
- Carbone, M., Agnello, C. & Alvarado, P. (2014) Phylogenetic and morphological studies in the genus Pseudoplectania (Ascomycota, Pezizales). Ascomycete.org 6: 17–33.
- Carbone, M., Agnello, C. & Alvarado, P. (2013a) Phylogenetic studies in the family Sarcosomataceae (Ascomycota, Pezizales). Ascomycete.org 5: 1–12. https://doi.org/10.25664/art-0075
- Carbone, M., Agnello, C. & Baglivo, A. (2009) Plectania mediterranea una nuova specie dell’Italia mediterranea, con storia e circoscrizione del Genere Plectania. Rivista di Micologia 52: 245–266.
- Carbone, M., Agnello, C., Baglivo, A., Perić, B. & Giorgi, D.D. (2011) Studio comparator delle specie lungamente stipitate del genere plectania. Mycologia Montenegrina 14: 7–38.
- Carbone, M., Agnello, C. & Parker, A. (2013b) Urnula padeniana (Pezizales) sp. nov. and the type study of Bulgaria mexicana. Ascomycete.org 5: 13–24. https://doi.org/10.25664/art-0076
- Carbone, M., Agnello, C., Parker, A. & Alvarado, P. (2021) Plectania harnischii, a new North American species of the Plectania melastoma lineage (Pezizales, Sarcosomataceae). Ascomycete.org 13 (6): 217–226. https://doi.org/10.25664/ART-0339
- Dissing, H. (1981) Four new species of Discomycetes (Pezizales) from West Greenland. Mycologia 73: 263–273. https://doi.org/10.1080/00275514.1981.12021343
- Ellis, J.B. & Holway, E.W.D. (1897) Mexican fungi. Botanical Gazette 24: 23–38. https://doi.org/10.1086/327549
- Fries, E.M. (1849) Summa vegetabilium Scandinaviae, Sectio posterior. Stockholm, Leipzig: Bonnier, pp. 259–572.
- Fries, E.M. (1851) Novae symbolae mycologicae, in peregrinis terries a botanicis Danicis collectae. Nova Acta Regiae Societatis Scientiarum Upsaliensis Series 3, 1: 17–136. https://doi.org/10.5962/bhl.title.112967
- Gardes, M. & Bruns, T.D. (1993) ITS primers with enhanced specificity for basidiomycetes application to the identification of mycorrhizae and rusts. Molecular Ecology 2: 113–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
- Guindon, S., Dufayard, J.F., Lefort, V., Anisimova, M., Hordijk, W. & Gascuel, O. (2010) New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Systematic Biology 59: 307–321. https://doi.org/10.1093/sysbio/syq010
- Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.
- Hazsl. (1887) Einige neue oder wenig bekannte Discomyceten. Verhandlungen der Kaiserlich-Königlichen Zoologisch-Botanischen Gesellschaft in Wien 37: 151–168.
- Healy, R.A., Arnold, A.E., Bonito, G., Huang, Y.L., Lemmond, B., Pfister, D.H. & Smith, M.E. (2021) Endophytism and endolichenism in Pezizomycetes: the exception or the rule? New Phytologist 233 (5): 194–1983. https://doi.org/10.1111/nph.17886
- Hoang, D.T., Chernomor, O., Von Haeseler, A., Minh, B.Q. & Vinh, L.S. (2018) UFBoot2: improving the ultrafast bootstrap approximation. Molecular Biology and Evolution 35: 518–522. https://doi.org/10.1093/molbev/msx281
- Huffman, D.M., Tiffany, L.H., Knaphus, G., Healy, R.A. (2008) Mushrooms and other fungi of the midcontinental United States, 2nd ed. University of Iowa Press, Iowa City. 370 pp. https://doi.org/10.2307/j.ctt20ks0sv
- Index Fungorum (2023) Available from: https://www.indexfungorum.org/names/Names.asp (accessed 4 Oct. 2023)
- Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) (2012) PCR protocols: a guide to methods and applications. Academic press.
- Jayasiri, S.C., Hyde, K.D., Ariyawansa, H.A., Bhat, J., Buyck, B., Cai, L., Dai, Y.C., Abd-Elsalam, K.A., Ertz, D., Hidayat, I., Jeewon, R., Jones, E.B.G., Bahkali, A.H., Karunarathna, S.C., Liu, J.K., Luangsa-ard, J.J., Lumbsch, H.T., Maharachchikumbura, S.S.N., McKenzie, E.H.C., Moncalvo, J.M., Ghobad-Nejhad, M., Nilsson, H., Pang, K.L., Pereira, O.L., Phillips, A.J.L. Raspé, O., Rollins, A.W., Romero, A.I., Etayo, J., Selçuk, F., Stephenson, S.L., Suetrong, S., Taylor, J.E., Tsui, C.K.M., Vizzini, A., Abdel-Wahab, M.A., Wen, T.C., Boonmee, S., Dai, D.Q., Daranagama, D.A., Dissanayake, A.J., Ekanayaka, A.H., Fryar, S.C., Hongsanan, S., Jayawardena, R.S., Li, W.J., Perera, R.H., Phookamsak, R., de Silva, N.I., Thambugala, K.M., Tian, Q., Wijayawardene, N.N., Zhao, R.L., Qi Zhao, Q., Kang, J.C. & Promputtha, I. (2015) The Faces of Fungi database: fungal names linked with morphology, phylogeny and human impacts. Fungal diversity 74: 3–18. https://doi.org/10.1007/s13225-015-0351-8
- Kaounas, V., Tsampazis, T. & Agnello, C. (2015) Urnula mediterranea (Pezizales), a rare species, recorded in Greece. Ascomycete.org 7: 97–100. https://doi.org/10.25664/art-0131
- Katoh, K., Rozewicki, J. & Yamada, K.D. (2019) MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings in bioinformatics 20: 1160–1166. https://doi.org/10.1093/bib/bbx108
- Kornerup, A. & Wanscher, J.H. (1967) Methuen handbook of colour.
- Kuraku, S., Zmasek, C.M., Nishimura, O. & Katoh, K. (2013) aLeaves facilitates on-demand exploration of metazoan gene family trees on MAFFT sequence alignment server with enhanced interactivity. Nucleic Acids Research 41: W22–W28. https://doi.org/10.1093/nar/gkt389
- Lu, J.R., Yu, F.M., LEI, L., Zhang, Y. & Zhao, Q. (2023) A new species of Helvella (Helvellaceae, Pezizomycetes) within the H. macropus group from China. Phytotaxa 601 (3): 212–222. https://doi.org/10.11646/phytotaxa.601.3.2
- Nylander, J.A.A. (2004) MrModeltest v2. Program distributed by the author Evolutionary Biology Centre, Uppsala University.
- Rambaut, A. (2018) FigTree—Tree Figure Drawing Tool Version v. 1.4. 4. Institute of Evolutionary Biology, University of Edinburgh: Edinburgh.
- Paden, J.W., Tylutki, E.E. (1969) Idaho Discomycetes. II. Mycologia 61: 683–693. https://doi.org/10.1080/00275514.1969.12018788
- Paden, J.W. (1983) Sarcosomataceae (Pezizales, Sarcoscyphineae). Flora Neotropica 37: 1–17.
- Rannala, B. & Yang, Z. (1996) Probability distribution of molecular evolutionary trees: a new method of phylogenetic inference. Journal of molecular evolution 43: 304–311. https://doi.org/10.1007/BF02338839
- Rehm, H. (1914) Ascomycetes philippinenses—VI. Leaflets of Philippine Botany 6: 2257–2281.
- Saccardo, P.A. (1889) Discomyceteae et Phymatosphaeriaceae. Sylloge Fungorum 8: 1–1143.
- Strobel, G., Ericksen, A., Sears, J., Xie, J., Geary, B. & Blatt, B. (2017) Urnula sp., an endophyte of Dicksonia antarctica, making a fragrant mixture of biologically active volatile organic compounds. Microbial ecology 74: 312–321. https://doi.org/10.1007/s00248-017-0947-5
- Trifinopoulos, J., Nguyen, L.T., von Haeseler, A. & Minh, B.Q. (2016) W-IQ-TREE: a fast online phylogenetic tool for maximum likelihood analysis. Nucleic Acids Research 44: W232–W235. https://doi. org/10.1093/nar/gkw256
- Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
- Wang, X.H., Das, K., Horman, J., Antonin, V., Baghela, A., Chakraborty, D., Hembrom, M.E., Nakasone, K., Ortiz-Santana, B., Vizzini, A., Hofstetter, V. & Buyck, B. (2018) Fungal biodiversity profiles 51–60. Cryptogamie Mycologie 39: 211–257. https://doi.org/10.7872/crym/v39.iss2.2018.211
- Wang, Y.Z. & Huang, C.L. (2015) A new species of Urnula (Sarcosomataceae, Pezizales) from Taiwan. Nova Hedwigia 101: 49–55. https://doi.org/10.1127/nova_hedwigia/2014/0233
- White, T.J., Bruns, T., Lee, S.J.W.T. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications 18: 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
- Zeng, M., Gentekaki, E., Hyde, K.D. & Zhao, Q. (2021) Donadinia echinacea and Plectania sichuanensis, two novel species of Sarcosomataceae from southwestern China. Phytotaxa 508: 1–21. https://doi.org/10.11646/phytotaxa.508.1.1
- Zettur, I. & Kullman, B. (2011) Urnula hiemalis—a rare and interesting species of the Pezizales from Estonia. Folia Cryptogamica Estonica 48: 149–152.
- Zhuang, W.X. (2004) Flora Fungorum Sinicorum. Vol. 21. Hyaloscyphaceae Sarcoscyphaceae et Sarcosomataceae. Science Press. [In Chinese]
- Zhuang, W.X., Zheng, H. & Zeng, Z.Q. (2018) Species Catalogue of China. Vol. 3. Fungi, Cup-Fungi. Science Press, pp. 111. [In Chinese]