Abstract
A new species of Moelleriella, described as Moelleriella simaoensis from Pu’er City, Yunnan Province, was discovered during a diversity survey of entomopathogenic fungi in Southwestern China. It was characterized by producing brightly colored and thin-pulvate stromata, subglobose to ovoid perithecia, cylindrical asci, disarticulating part-spores inside the ascus, and fusiform conidia. Both anamorphic and teleomorphic states were most often found on the same stroma, and the orange conidiomata were aggregated in the center of the stroma. Conidiomata were widely open with several locules per stroma. Multigenes phylogenetic analyses based on nrLSU, tef-1α and rpb1 sequences showed that this species formed a distinct clade and was closely related to Moelleriella puerensis and Moelleriella raciborskii. The Description and illustration of the new species were provided in the present study.
References
Bischoff, J.F. , Rehner, S.A. & Humber, R.A. (2006) Metarhizium frigidum sp nov.: a cryptic species of M. anisopliae and a member of the M. flavoviride complex. Mycologia 98: 737–745. https://doi.org/10.3852/mycologia.98.5.737
Berger, E.W. (1921) Natural enemies of scale insects and whiteflies in Florida. Florida State Plant Board Quarterly Bulletin 5: 141–154.
Chaverri, P., Bischoff, J.F. & Hodge, K.T. (2005b) A new species of Hypocrella, H. macrostroma, and its relationship to other species with large stromata. Mycological Research 109: 126–1275. https://doi.org/10.1017/S0953756205003904
Chaverri, P., Liu, M. & Hodge, K.T. (2008) A monograph of the entomopathogenic genera Hypocrella, Moelleriella, and Samuelsia gen. nov. (Ascomycota, Hypocreales, Clavicipitaceae), and their Aschersonia-like anamorphs in the Neotropics. Studies in Mycology 60: 1–66. https://dx.doi.org/10.3114/sim.2008.60.01
Dai, Y.C., Cui, B.K., Si, J., He, S.H., Hyde, K.D., Yuan, H.S., Liu, X.Y. & Zhou, L.W. (2015) Dynamics of the worldwide number of fungi with emphasis on fungal diversity in China. Mycological Progress 14: 62. https://doi.org/10.1007/s11557-015-1084-5
Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature Methods 9: 772. https://doi.org/10.1038/nmeth.2109
Evans, H.C. & Hywel-Jones, N. (1990) Aspects of the genera Hypocrella and Aschersonia as pathogens of coccids and whiteflies. In: Vth International Colloquium on Invertebrate Pathology and microbial Control. Society for Invertebrate Pathology, Adelaide, Australia. pp. 111–115.
Hyde, K.D., Norphanphoun, C., Chen, J., Dissanayake, A.J., Doilom, M., Hongsanan, S., Jayawardena, R.S., Jeewon, R., Perera, R.H., Thongbai, B., Wanasinghe, D.N., Wisitrassameewong, K., Tibpromma, S. & Stadler, M. (2018) Thailand’s amazing diversity: up to 96% of fungi in northern Thailand are novel. Fungal Diversity 93: 215–239.
Hywel-Jones, N.L. & Evans, H.C. (1993) Taxonomy and ecology of Hypocrella discoidea and its anamorph, Aschersonia samoensis. Mycological Research 97: 871–876. https://doi.org/10.1016/S0953-7562(09)81165-9
Khonsanit, A., Noisripoom, W., Mongkolsamrit, S., Phosrithong, N. & Luangsa-Ard, J.J. (2021) Five new species of Moelleriella infecting scale insects (coccidae) in thailand. Mycological Progress 20: 847–267. https://doi.org/10.1007/s11557-021-01709-5
Kornerup, A. & Wanscher, J.H. (1978) Handbook of colour. Methuen, London. pp. 1–252.
Liu, Z.Y., Liang, Z.Q., Whalley, A.J.S., Yao, Y.J. & Liu, A.Y. (2001) Cordyceps brittlebankisoides, a new pathogen of grubs and its anamorph, Metarhizium anisopliae var. majus. Journal of Invertebrate Pathology 78: 178–182. https://doi.org/10.1006/jipa.2001.5039
Liu, M. & Hodge, K.T. (2005) Hypocrella zhongdongii sp. nov., the teleomorph of Aschersonia incrassata. Mycological Research 109: 818–824. https://doi.org/10.1016/10.1017/S095375620500290X
Li, G.J., Hyde, K.D., Zhao, R.L., Hongsanan, S., Abdel-Aziz, F.A., Abdel-Wahab, M.A., Alvarado, P., Alves-Silva, G., Ammirati, J.F., Ariyawansa, H.A., Baghela, A., Bahkali, A.H., Beug, M., Bhat, D.J., Bojantchev, D., Boonpratuang, T., Bulgakov, T.S., Camporesi, E., Boro, M.C., Ceska, O., Chakraborty, D., Chen, J.J., Chethana, K.W.T., Chomnunti, P., Consiglio, G., Cui, B.K., Dai, D.Q., Dai, Y.C., Daranagama, D.A., Das, K., Dayarathne, M.C., De Crop, E.D., De Oliveira, R.J.V., de Souza, C.A.F., de Souza, J.I., Dentinger, B.T.M., Dissanayake, A.J., Doilom, M., Drechsler-Santos, E.R., Ghobad-Nejhad, M., Gilmore, S.P., Góes-Neto, A., Gorczak, M., Haitjema, C.H., Hapuarachchi, K.K., Hashimoto, A., He, M.Q., Henske, J.K., Hirayama, K., Iribarren, M.J., Jayasiri, S.C., Jayawardena, R.S., Jeon, S.J., Jerônimo, G.H., Jesus, A.L., Jones, E.B.G., Kang, J.C., Karunarathna, S.C., Kirk, P.M., Konta, S., Kuhnert, E., Langer, E., Lee, H.S., Lee, H.B., Li, W.J., Li, X.H., Liimatainen, K., Lima, D.X., Lin, C.G., Liu, J.K., Liu, X.Z., Liu, Z.Y., Luangsa-ard, J.J., Lücking, R., Lumbsch, H.T., Lumyong, S., Leaño, E.M., Marano, A.V., Matsumura, M., McKenzie, E.H.C., Mongkolsamrit, S., Mortimer, P.E., Nguyen, T.T.T., Niskanen, T., Norphanphoun, C., O’Malley, M.A., Parnmen, S., Pawłowska, J., Perera, R.H., Phookamsak, R., Phukhamsakda, C., Pires-Zottarelli, C.L.A., Raspé, O., Reck, M.A., Rocha, S.C.O., de Santiago Cabral Monteiro, A.L., Senanayake, I.C., Setti, L., Shang, Q.J., Singh, S.K., Sir, E.B., Solomon, K.V., Song, J., Srikitikulchai, P., Stadler, M., Suetrong, S., Takahashi, H., Takahashi, T., Tanaka, K., Tang, L.P., Thambugala, K.M., Thanakitpipattana, D., Theodorou, M.K., Thongbai, B., Thummarukcharoen, T., Tian, Q., Tibpromma, S., Verbeken, A., Vizzini, A., Vlasák, J., Voigt, K., Wanasinghe, D.N., Wang, Y., Weerakoon, G., Wen, H.A., Wen, T.C., Wijayawardene, N.N., Wongkanoun, S., Wrzosek, M., Xiao, Y.P., Xu, J.C., Yan, J.Y., Yang, J., Yang, S.D., Hu, Y., Zhang, J.F., Zhao, J., Zhou, L.W., Peršoh, D., Phillips, A.J.L. & Maharachchikumbura, S.S.N. (2016) Fungal diversity notes 253–366: taxonomic and phylogenetic contributions to fungal taxa. Fungal Diversity 78: 1–237. https://doi.org/10.1007/s13225-016-0366-9
Liu, M., Chaverri, P. & Hodge, K.T. (2006) A taxonomic revision of the insect biocontrol fungus Aschersonia aleyrodis, its allies with white stromata and their Hypocrella sexual states. Mycol Research 110: 537–554. https://doi.org/10.1016/j.mycres.2006.01.013
Larkin, M.A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan, P.A., McWilliam, H., Valentin, F., Wallace, I.M., Wilm, A., Lopez, R., Thompson, J.D., Gibson, T.J. & Higgins, D.G. (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23 (21): 2947–2948. https://doi.org/10.1093/bioinformatics/btm404
Mains, E.B. (1959a) Species of Aschersonia (Sphaeropsidales). Lloydia 22: 215–221.
Mains, E.B. (1959b) Species of Hypocrella. Mycopathologia et Mycologia Applicata 11: 311–326.
Mongkolsamrit, S., Luangsa-ard, J.J. & Hywel-Jones, N.L. (2011a) Samuelsia mundiveteris sp. nov. from Thailand. Mycologia 103: 921–927. https://doi.org/10.3852/11-049
Mongkolsamrit, S., Nguyen, T.T., Tran, N.L. & Luangsa-ard, J.J. (2011b) Moelleriella pumatensis, new entomogenous species from Vietnam. Mycotaxon 117: 45–51. https://doi.org/10.5248/117.45
Petch, T. (1921) Studies in entomogenous fungi. II The genera of Hypocrella and Aschersonia. Annals of the Royal Botanic Gardens (Peradeniya) 7: 167–278.
Petch, T. (1932) Notes on entomogenous fungi. Transactions of the British Mycological Society 16: 209–245.
Rehner, S.A. & Samuels, G.J. (1994) Taxonomy and phylogeny of Gliocladium analysed from nuclear large subunit ribosomal DNA sequences. Mycological Research 98: 625–634. https://doi.org/10.1016/S0953-7562(09)80409-7
Samson, R.A. & Rombach, M.C. (1985) Biology of the fungi Verticillium and Aschersonia. In: Hussey, N.W. & Scopes, N. (eds.) Biological pest control: the glasshouse experience. Cornell University Press, Ithaca, New York. pp. 34–42.
Sung, G.H., Hywel-Jones, N.L., Sung, J.M., Luangsa-Ard, J.J., Shrestha, B. & Spatafora, J.W. (2007) Phylogenetic classifcation of Cordyceps and the clavicipitaceous fungi. Studies in Mycololgy 57: 5–59. https://doi.org/10.3114/sim.2007.57.01
Tadych, M., Chaverri, P., Bergen, M. & White, J.F. (2009) Moelleriella zhongdongii stroma development and identification of hirsutella-like and Aschersonia synanamorphs. Mycological Research 113 (5): 611–615. https://doi.org/10.1016/j.mycres.2009.01.011
Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution 30: 2725–2729. https://dx.doi.org/10.1093/molbev/mst197
Vilgalys, R. & Hester, M. (1990) Rapid genetic identifcation and mapping of enzymatically amplifed ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
Webber, H.J. (1897) Sooty mold of the orange and its treatment. Bulletin of the U.S. Department of Agriculture Division of Vegetable Physiology and Pathology 13: 1–34.
Wang, Y.B., Yu, H., Dai, Y.D., Chen, Z.H., Zeng, W.B., Yuan, F. & Liang, Z.Q. (2015b) Polycephalomyces yunnanensis (Hypocreales), a new species of Polyceph- alomyces parasitizing Ophiocordyceps nutans and stink bugs (hemipteran adults). Phytotaxa 208: 34–44. https://doi.org/10.11646/phytotaxa.208.1.3
Wang, Y.B., Wang, Y., Fan, Q., Duan, D.E., Zhang, G.D., Dai, R.Q., Dai, Y.D., Zeng, W.B., Chen, Z.H., Li, D.D., Tang, D.X., Xu, Z.H., Sun, T., Nguyen, T.T., Tran, N.L., Dao, V.M., Zhang, C.M., Huang, L.D., Liu, Y.J., Zhang, X.M., Yang, D.R., Sanjuan, T., Liu, X.Z., Yang, Z.L. & Yu, H. (2020) Multigene phylogeny of the family Cordycipitaceae (Hypocreales): new taxa and the new systematic position of the Chinese cordycipitoid fungus Paecilomyces hepiali. Fungal Diversity 103: 1–46. https://doi.org/10.1007/s13225-020-00457-3