Abstract
Argostemma iriomotense, previously considered a synonym of A. solaniflorum shortly after its publication, is hereby reinstated based on both morphological and molecular evidence. It can be distinguished morphologically from the latter species by its dwarf plant size, stipules measuring less than 2 mm, leaves which are sparsely strigillose along veins or glabrescent adaxially, and brochidodromous secondary veins that are slightly elevated adaxially and slightly grooved abaxially. Through phylogenetic analysis, samples of A. iriomotense collected from its type locality, together with a newly discovered population in southern Taiwan, form a distinct group separate from A. solaniflorum. These findings support the reinstatement of A. iriomotense from A. solaniflorum. A comprehensive description of its morphology, accompanied by relevant photographs, is provided in this study.
References
- Bennet, J.J. (1838) Plantae Javanicae Rariores, Descriptae Iconibus Illustratae, quas in Insula Java, Annis 1802-1818, Legit et Investigatit Thomas Horsefield, M.D. e Siccis Descriptiones et Characteres Plurimarum Elaboravit Joannes J. Bennett; Observationes Structuram et Affinitates Praesertim Respicientes Passim Adjecit Robertus Brown. Part 1. W.H.Allen, London, 104 pp.
- Bennet, S.S.R. (1981) Nomenclatural Changes in some Flowering Plants. Indian Forester 107: 436.
- Blasco, F.A., Tandang, D.N. & Alejandro, G.J.D. (2024) Argostemma mirae (Rubiaceae) a new species from Tago, Surigao del Sur, Mindanao Island, Philippines. Phytotaxa 640 (1): 65–70. https://doi.org/10.11646/phytotaxa.640.1.7
- Bremer, B. (1989) The genus Argostemma (Rubiaceae—Argostemmateae) in Borneo. Annals of the Missouri Botanical Garden 76: 7–49. https://doi.org/10.2307/2399341
- Bremer, B., Bremer, K., Heidari, N., Erixon, P., Olmstead, R.G., Anderberg, A.A., Kallersjo, M. & Barkhordarian, E. (2002) Phylogenetics of asterids based on 3 coding and 3 non-coding chloroplast DNA markers and the utility of non-coding DNA at higher taxonomic levels. Molecular phylogenetics and evolution 24 (2): 274–301. https://doi.org/10.1016/S1055-7903(02)00240-3
- Chao, J.-M. (1978) Rubiaceae. In: Li, H.-L., Liu, T.-S., Huang, T.-C., Koyama, T. & Devol, C.E. (Eds.) Flora of Taiwan, vol. 4. Epoch Publishing Co., Ltd., Taipei, pp. 247–346.
- Chen, T. & Taylor, C.M. (2011) Argostemma. In: Wu, Z.-Y. & Raven, P.H. (Eds.) Flora of China, vol. 19. Science Press, Beijing & Missouri Botanical Garden Press, St. Louis, pp. 75–76.
- Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: More models, new heuristics and parallel computing. Nature Methods 9: 772. https://doi.org/10.1038/nmeth.2109
- Doyle, J.J. & Doyle, J.L. (1990) Isolation of plant DNA from fresh tissue. Focus 12 (13): 39–40.
- Edgar, R.C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic acids research 32 (5): 1792–1797. https://doi.org/10.1093/nar/gkh340
- Elmer, A.D.E. (1906) Philippine Rubiaceae. Leaflets of Philippine Botany. Leaflets of Philippine Botany 1: 2.
- Farris, J.S., Kallersjo, M., Kluge, A.G. & Bult, C. (1994) Testing significance of incongruence. Cladistics 10: 315–319. https://doi.org/10.1111/j.1096-0031.1994.tb00181.x
- Hatusima, S. (1956) New and Noteworthy Plants from the Ryukyu Islands and Formosa II. Bulletin of Arts & Science Division, University of the Ryukyus. Mathematics & Natural Sciences 3: 106–113
- Hatusima, S. (1971) Flora of the Ryukyus. Okinawa Seibutsu Kyoiku Kenkyukai, Naha. pp. 582–583.
- Huang, S.-F. & Huang, T.-C. (1997) Notes on the flora of Taiwan (27)—The genus Argostemma Wall. (Rubiaceae). Taiwania 42 (1): 1–7.
- Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. (2018) MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution 35: 1547–1549. https://doi.org/10.1093/molbev/msy096
- Masamune, G. (1935) Beitrage zur Kenntnis der Flora von Südjapan (IV). Transactions of the Natural History Society of Formosa 25: 248.
- Masamune, G. (1938) Miscellaneous notes on the flora of the eastern Asia IV. Transactions of the Natural History Society of Formosa 28: 234.
- Merrill, E.D. (1923) Diagnosis of Hainan plants, II. Philippine Journal of Science 23: 265–266.
- Oxelman, B., Lidén, M. & Berglund, D. (1997) Chloroplast rps16 intron phylogeny of the tribe Sileneae (Caryophyllaceae). Plant systematics and Evolution 206: 393–410. https://doi.org/10.1007/BF00987959
- Pitard, J. (1922) Rubiaceae. In: Lecomte, H. (Ed.) Flore Générale de l’Indo-Chine. Vol 3. Masson, Paris, pp. 20–144.
- Reinwardt, C.G.C. (1828) Nova plantarum indicarum genera. In: Hornschuch, C.F. (Eds.) Sylloge Plantarum Novarum Itemque Minus Cognitarum a Praestantissimis Botanicis adhuc Viventibus Collecta et a Societate Regia Botanica Ratisbonensi Edita. Vol. 2. Societate Regia Botanica, Regensburg, pp. 9.
- Ridley, H.N. (1927) The Genus Argostemma. Journal of Botany, British and Foreign 65: 25–41.
- Rydin, C., Razafimandimbison, S.G. & Bremer, B. (2008) Rare and enigmatic genera (Dunnia, Schizocolea, Colletoecema), sisters to species-rich clades: phylogeny and aspects of conservation biology in the coffee family. Molecular Phylogenetics and Evolution 48 (1): 74–83. https://doi.org/10.1016/j.ympev.2008.04.006
- Rydin, C., Razafimandimbison, S.G., Khodabandeh, A. & Bremer, B. (2009) Evolutionary relationships in the Spermacoceae alliance (Rubiaceae) using information from six molecular loci: insights into systematic affinities of Neohymenopogon and Mouretia. Taxon 58 (3): 793–810. https://doi.org/10.1002/tax.583009
- Ryukyu Plant Research Group (2018) Database of Ryukyu plants, National Museum of Nature and Science, Japan. Available from: https://www.kahaku.go.jp/research/activities/project/hotspot_japan/ryukyus/db/ (accessed: 16 May 2023)
- Shaw, J., Lickey, E.B., Beck, J.T., Farmer, S.B., Liu, W., Miller, J., Siripun, K.C., Winder, C.T., Schilling, E.E. & Small, R.L. (2005) The tortoise and the hare II: relative utility of 21 noncoding chloroplast DNA sequences for phylogenetic analysis. American journal of botany 92 (1): 142–166. https://doi.org/10.3732/ajb.92.1.142
- Suchard, M.A., Lemey, P., Baele, G., Ayres, D.L., Drummond, A.J. & Rambaut, A. (2018) Bayesian phylogenetic and phylodynamic data integration using BEAST 1.10. Virus evolution 4 (1): vey016. https://doi.org/10.1093/ve/vey016
- Swofford, D.L. (2002) PAUP* Phylogenetic analysis using parsimony (*and other methods), v. 4.0b10. Sunderland: Sinauer Associates.
- Taberlet, P., Gielly, L., Pautou, G. & Bouvet, J. (1991) Universal primers for amplification of three non-coding regions of. Plant molecular biology 17: 1105–1109. https://doi.org/10.1007/BF00037152
- Walker, E.H. (1976) Flora of Okinawa and the southern Ryukyu Islands. Smithsonian Institution Press, Washington, USA. 1159 pp.
- Wallich, N. (1824) Flora Indica: or Descriptions of Indian plants. Vol. 2. The Mission Press, Serampore, 588 pp.
- Yamazaki, T. (1993) Rubiaceae. In: Iwatsuki, K., Yamazaki, T., Boufford, D.E. & Ohba, H. (Eds.) Flora of Japan, Volume 3a. Kodansha, Tokyo, Japan. pp. 206–240.
- Yang, T.Y.A (1998) Argostemma Wall. In: Huang, T.-C. (Eds.) Flora of Taiwan, 2nd ed. vol. 4. Editorial Committee of the flora of Taiwan. Department of Botany, National Taiwan University, Taipei. pp. 246–247.
- Ying, S.S. (1989) Miscellaneous notes on the flora of Taiwan (XII). Memoirs of the College of Agriculture, National Taiwan University 29 (2): 56–57.