Abstract
Lyophyllum deqinense sp. nov. is described and illustrated from Yunnan Province, China, based on morphological features and molecular (ITS and LSU) data. The species can be distinguished by a greyish brown to light brown pileus and obtuse central umbo of the pileus, inrolled margin, globose to subglobose basidiospores (5.7–7.2 × 5.5–6.8 µm), clavate basidia (24–34 × 7–10 µm), cespitose and gregarious habit in soil. L. pallidofumosum is similar to the new species in pileus and stipe color, and shape and size of the basidia, but it lacks an obtuse central umbo, has smaller spores (4.5–5.6 × 4.3–5.4 µm), and sometimes is solitary or subcespitose. The combination of morphological characteristics and phylogenetic analyses indicates that the species belongs to the L. decastes complex.
References
Cooper, J.A. (2014) New species and combinations of some New Zealand agarics belonging to Clitopilus, Lyophyllum, Gerhardtia, Clitocybe, Hydnangium, Mycena, Rhodocollybia and Gerronema. Mycosphere 5: 263–288. https://doi.org/10.5943/mycosphere/5/2/2
Darriba, D., Taboada, G., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature Methods 9: 772. https://doi.org/10.1038/nmeth.2109
Endo, N., Ushijima, S., Nagasawa, E., Sugawara, R., Okuda, Y., Sotome, K., Nakagiri, A. & Maekawa, N. (2019) Taxonomic reconsideration of Tricholoma foliicola (Agaricales, Basidiomycota) based on basidiomata morphology, living culture characteristics, and phylogenetic analyses. Mycoscience 60: 323–330. https://doi.org/10.1016/j.myc.2019.07.002
Fries, E.M. (1821) Systema Mycologicum. Vol. 1. Ex Officina Berlingiana, Lundae, 520 pp.
Guindon, S. & Gascuel, O. (2003) A Simple, Fast, and Accurate Algorithm to Estimate Large Phylogenies by Maximum Likelihood. Systematic biology 52: 696–704. https://doi.org/10.1080/10635150390235520
Hall, T.A. (1999) Bioedit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/ NT. Nucleic Acids Symposium Series 41: 95–98.
Hofstetter, V., Clémençon, H., Vilgalys, R. & Moncalvo, J.-M. (2002) Phylogenetic analyses of the Lyophylleae (Agaricales, Basidiomycota) based on nuclear and mitochondrial rDNA sequences. Mycological Research 106: 1043–1059. https://doi.org/10.1017/S095375620200641X
Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular biology and evolution 30: 772–780. https://doi.org/10.1093/molbev/mst010
Kornerup, A. & Wanscher, J.H. (1978) Methuen handbook of colour. Methuen, London, 252 pp.
Kozlov, A.M., Darriba, D., Flouri, T., Morel, B. & Stamatakis, A. (2019) RAxML-NG: a fast, scalable and user-friendly tool for maximum likelihood phylogenetic inference. Bioinformatics (Oxford, England) 35: 4453–4455. https://doi.org/10.1093/bioinformatics/btz305
Larsson, E. & Sundberg, H. (2011) Lyophyllum shimeji, a species associated with lichen pine forest in northern Fennoscandia. Mycoscience: 289–295. https://doi.org/10.1007/s10267-010-0104-1
Li, T., Li, T.H., Wang, C.Q., Deng, W.Q. & Song, B. (2017) Gerhardtia sinensis (Agaricales, Lyophyllaceae), a new species and a newly recorded genus for China. Phytotaxa 332: 172–180. https://doi.org/10.11646/phytotaxa.332.2.4
Li, T.H. & Zhang, W.M. (1991) A study of Lyophyllum fumosum and its synonyms. Edible Fungi of China 10: 35+37.
Li, X. & Li, Y. (2009) Present status and future prospects of Lyophyllum Karst. in China. Acta Edulis Fungi 16: 75–79.
Liu, P.G. (1993) Introduction of a valuable edible fungus from Yunnan—Lyophyllum shimeji (Kaeam.) Hongo. Edible Fungi of China 12: 29.
Ma, Y.H., Liu, P., Zhao, Z.Y., Chen, W.M. & Zhao, Y.C. (2022) Lyophyllum pallidofumosum sp. nov. (Lyophyllaceae, Agaricales), from southwestern China. Phytotaxa 576: 173–183. https://doi.org/10.11646/phytotaxa.576.2.3
Moncalvo, J.-M. & Clémençon, H. (1994) Enzymic studies as an aid to the taxonomy of the Lyophyllum decastes complex. Mycological Research 98: 375–383. https://doi.org/10.1016/S0953-7562(09)81192-1
Moncalvo, J.-M., Lutzoni, F.M., Rehner, S.A., Johnson, J. & Vilgalys, R. (2000) Phylogenetic Relationships of Agaric Fungi Based on Nuclear Large Subunit Ribosomal DNA Sequences. Systematic biology 49: 278–305. https://doi.org/10.1093/sysbio/49.2.278
Osmundson, T., Robert, V., Schoch, C., Baker, L., Smith, A., Robich, G., Mizzan, L. & Garbelotto, M. (2013) Filling Gaps in Biodiversity Knowledge for Macrofungi: Contributions and Assessment of an Herbarium Collection DNA Barcode Sequencing Project. PloS one 8: e62419. https://doi.org/10.1371/journal.pone.0062419
Persoon, C.H. & Besemann, C.A. (1801) Synopsis methodica fungorum. Sistens enumerationem omnium huc vsque detectarum specierum, cum brevibus descriptionibus nec non synonymis et observationibus selectis. Apud Henricum Dietrich, Gottingae, 706 pp. https://doi.org/10.5962/bhl.title.166151
Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic biology 61: 539–542. https://doi.org/10.1093/sysbio/sys029
Sánchez-García, M. & Matheny, P.B. (2017) Is the switch to an ectomycorrhizal state an evolutionary key innovation in mushroom-forming fungi? A case study in the Tricholomatineae (Agaricales). Evolution 71: 51–65. https://doi.org/10.1111/evo.13099
Singer, R. (1986) The Agaricales in Modern Taxonomy. Koeltz Scientific Books, Königstein, 1069 pp.
Sundberg, H. (2010) Phylogeny of Lyophyllum section Difformia Does hon-shimeji (L. shimeji) occur in Sweden? Master thesis of Department of Plant and Environmental Sciences, University of Gothenburg, Sweden, 19 pp.
Vaidya, G., Lohman, D.J. & Meier, R. (2011) SequenceMatrix: concatenation software for the fast assembly of multi-gene datasets with character set and codon information. Cladistics: the international journal of the Willi Hennig Society 27: 171–180. https://doi.org/10.1111/j.1096-0031.2010.00329.x
Varga, T., Krizsán, K., Földi, C., Dima, B., Sánchez-García, M., Sánchez-Ramírez, S., Szöllősi, G.J., Szarkándi, J.G., Papp, V., Albert, L., Andreopoulos, W., Angelini, C., Antonín, V., Barry, K.W., Bougher, N.L., Buchanan, P., Buyck, B., Bense, V., Catcheside, P., Chovatia, M., Cooper, J., Dämon, W., Desjardin, D., Finy, P., Geml, J., Haridas, S., Hughes, K., Justo, A., Karasiński, D., Kautmanova, I., Kiss, B., Kocsubé, S., Kotiranta, H., LaButti, K.M., Lechner, B.E., Liimatainen, K., Lipzen, A., Lukács, Z., Mihaltcheva, S., Morgado, L.N., Niskanen, T., Noordeloos, M.E., Ohm, R.A., Ortiz-Santana, B., Ovrebo, C., Rácz, N., Riley, R., Savchenko, A., Shiryaev, A., Soop, K., Spirin, V., Szebenyi, C., Tomšovský, M., Tulloss, R.E., Uehling, J., Grigoriev, I.V., Vágvölgyi, C., Papp, T., Martin, F.M., Miettinen, O., Hibbett, D.S. & Nagy, L.G. (2019) Megaphylogeny resolves global patterns of mushroom evolution. Nature Ecology & Evolution 3: 668–678. https://doi.org/10.1038/s41559-019-0834-1
Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
Walther, G., Garnica, S. & Weiß, M. (2005) The systematic relevance of conidiogenesis modes in the gilled Agaricales. Mycological Research 109: 525–544. https://doi.org/10.1017/S0953756205002868
Wei, S.W., Lu, B.Y., Wang, Y., Dou, W.J., Wang, Q. & Li, Y. (2023) Morphology and Phylogeny of Lyophylloid Mushrooms in China with Description of Four New Species. J Fungi (Basel) 9: 77. https://doi.org/10.3390/jof9010077
White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. &White, T.J. (eds.) PCR Protocols: a guide to methods and applications. Academic Press, San Diego, CA, pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1