Abstract
Elaphomyces readii is presented as a new species to science based on morphological and molecular evidence. The new species is characterized mainly by the pale brown rugose ascomata, yellowish to reddish brown hues in the proximity to the gleba, dark purple gleba and ascospores 20–57 µm in diameter. Elaphomyces readii is morphologically similar to E. papillatus and E. muricatus but differs in the ascospore size and phylogenetical location. Further, E. readii is sold in traditional markets in the state of Oaxaca, southeastern Mexico as a medicinal fungus consumed to heal wounds, internal bleeding, and external injuries. Morphological descriptions, SEM ornamentation spores and nrITS-based phylogenetic analysis using maximum parsimony, maximum likelihood method and Bayesian inference support the proposal of this new species.
References
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Buyck, B., Hosaka, K., Shelly, M. & Hofstetter, V. (2016) Molecular analyses of first collections of <em>Elaphomyces</em> Nees (Elaphomycetaceae, Eurotiales, Ascomycota) from Africa and Madagascar indicate that the current concept of <em>Elaphomyces</em> is polyphyletic. <em>Cryptogamie Mycologie</em> 37 (1): 3−14. https://doi.org/10.7872/crym/v37.iss1.2016.3</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Castellano, M.A., Trappe, J.M., Maser, Z. & Maser, S. (1986) <em>Key to spores of the genera of hypogeous fungi of North America, with reference to animal mycophagy.</em> Mad River Press, Eureka, 185 pp.</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Castellano, M.A., Guevara-Guerrero, G., García-Jiménez, J. & Trappe, J.M. (2012) <em>Elaphomyces appalachiensis</em> and <em>E</em>. <em>verruculosus</em> sp. nov. (Ascomycota Eurotiales, Elaphomycetaceae) from eastern North America.<em> Revista Mexicana de Micología</em> 35: 17−22.</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Castellano, M.A., Dentinger, B.T.M., Séné, O., Truong, C. & Henkel, T.W. (2016) New species of <em>Elaphomyces</em> (Elaphomycetaceae, Eurotiales, Ascomycota) from tropical rainforests of Cameroon and Guyana. <em>IMA Fungus</em> 7: 59–73. https://doi.org/10.5598/imafungus.2016.07.01.05</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Castellano, M.A., Elliott, T.F. & Trappe, J.M. (2018) Three new black <em>Elaphomyces</em> species (Elaphomycetaceae, Eurotiales, Ascomycota) from eastern North America with notes on selected European species.<em> Fungal Systematics and Evolution</em> 1 (1): 1−12. https://doi.org/10.3114/fuse.2018.01.01</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Castellano, M.A., Crabtree, C.D., Mitchell, D. & Healy, R.A. (2021) Eight new <em>Elaphomyces</em> species (Elaphomycetaceae, Eurotiales, Ascomycota) from eastern North America. <em>Fungal Systematics</em> <em>and Evolution</em> 7 (1): 113−131. https://doi.org/10.3114/fuse.2021.07.06</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Cázares, E., García, J., Castillo, J. & Trappe, J.M. (1992) Hypogeous Fungi from Northern Mexico. <em>Mycologia</em> 84 (3): 341–359. https://doi.org/10.2307/3760186</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Chauhan, O.P., Vijay, V., Pandey, A.K. & Semwal, A.D. (2021) Biochemical and Health Properties of Truffles. <em>Defence Life Science Journal </em>6 (3): 251–258. https://doi.org/10.14429/dlsj.6.15659</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">García-Romero, L., Guzmán, G. & Herrera, T. (1970) Especies de macromicetos citadas de México, I. Ascomycetes, Tremellales y Aphyllophorales. <em>Boletín de la Sociedad Mexicana de Micología </em>(4): 54–76. https://doi.org/10.33885/sf.1970.1.404</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Gómez-Reyes, V.M., Hernández-Salmerón, I.R., Terrón-Alfonso, A. & Guevara-Guerrero, G. (2012) Taxonomic study on <em>Elaphomyces</em> spp. (Ascomycota, Eurotiales, Elaphomycetaceae) from Michoacan, Mexico. <em>Revista Mexicana de Micología</em> 36: 57–82.</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Gómez-Reyes, V.M., Vázquez-Marrufo, G., Ortega Gómez, A.M. & Guevara Guerrero, G. (2018) Ascomicetos hipogeos de la región occidental del Sistema Volcánico Transversal, México.<em> Acta Botánica Mexicana</em> 125: 37–48. https://doi.org/10.21829/abm125.2018.1327</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Heim, R. (1957) Sur les psilocybes hallucinatoires des azteques et sur le microendémisme des agarics utilisées par les indiens du Mexique a des fins divinatoires. <em>Revue Mycologique </em>22 (3): 300–305. https://doi.org/10.3406/jatba.1956.2313</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Jeppson, M., Molia, A., Gustafsson, D. & Larsson, E. (2020) Fem för sverige nya hjorttryfflar (<em>Elaphomyces</em>). <em>Svensk Mykologisk Tidskrift</em> 41 (2): 2–11.</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Kornerup, A. & Wanscher, H. (1978) <em>Methuen handbook of colour.</em> 3rd edition. Eyre, London, 227 pp.</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Molia, A., Larsson, E., Jeppson, M., Læssøe, T. & Larsson, K-H. (2020) <em>Elaphomyces</em> section <em>Elaphomyces</em> (Eurotiales, Ascomycota)—taxonomy and phylogeny of North European taxa, with the introduction of three new species. <em>Fungal Systematic and Evolution</em> 5: 283–300. https://doi.org/10.3114/fuse.2020.05.14</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Paz, A., Bellanger, J.M., Lavoise, C., Molia, A., Ławrynowicz, M., Larsson, E., Ibarguen, I.O., Jeppson, M., Læssøe, T., Sauve, M., Richard, F. & Moreau, P.A. (2017) The genus <em>Elaphomyces</em> (Ascomycota, Eurotiales): a ribosomal DNA-based phylogeny and revised systematics of European ‘deer truffles’. <em>Persoonia</em> 38 (1): 197–239. https://doi.org/10.3767/003158517X697309</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Pegler, D.N., Spooner, B.M. & Young, T.W.K. (1993) <em>British truffles. A revision of British hypogeous fungi</em>. The Board of Trustees of the Royal Botanical Gardens, Kew, 215 pp.</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Pérez-Moreno, J. & Martínez-Reyes, M. (2014) Edible ectomycorrhizal mushrooms. Biofactories for sustainable development.<em> In: </em>Guevara-González, R. & Torres-Pacheco, I. (eds.) <em>Biosystems Engineering Biofactories for food production in the century XXI.</em> Springer, Berlin. pp. 151–233. https://doi.org/10.1007/978-3-319-03880-3_6</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Saltarelli, R., Ceccaroli, C.P., Barbieri, E. & Stocchi, V. (2008) Effect of storage on biochemical and microbiological parameters of edible truffle species. <em>Food Chemistry</em> 109: 8–16. https://doi.org/10.1016/j.foodchem.2007.11.075</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Shirakawa, M. & Tanaka, M. (2020) Two new deer truffle species, <em>Elaphomyces marmoratus</em> and <em>Elaphomyces fuscus</em> spp. nov., from a secondary forest in Japan. <em>Mycoscience</em> 61 (6): 315–322. https://doi.org/10.1016/j.myc.2020.07.002</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Trappe, J.M. & Guzmán, G. (1971) Notes on some hypogeous fungi from Mexico. <em>Mycologia</em> 63 (2): 317–332. https://doi.org/10.1080/00275514.1971.12019112</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Sukarno, N., Listiyowati, S., Rahayu, N. & Nara, K. (2019) <em>Elaphomyces tropicalis</em> sp. nov.: A new ectomycorrhizal fungus associated with dipterocarps from tropical Indonesia. <em>Mycoscience</em> 60 (2): 83–88. https://doi.org/10.1016/j.myc.2018.12.004</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Trappe, J.M., Guzmán, G. & Vázquez-Salinas, C. (1979) Observaciones sobre la identificación, distribución y usos de los hongos del género <em>Elaphomyces</em> en México. <em>Boletín de la Sociedad Mexicana de Micología</em> 13: 145–150.</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Vittadini, C. (1831) <em>Monographia tuberacearum. </em>Mediolani, 88 pp.</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">White, T.J., Bruns, T.D., Lee, S. & Taylor, J.W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics.<em> In: </em>Innis, M.A., Gelfand, D.H., Sninsky, J. & White, T.J. (Eds.) <em>PCR protocols: a guide to methods and applications.</em> Academic, San Diego, pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Wang, S. & Marcone, M.F. (2011) The biochemistry and biological properties of the world’s most expensive underground edible mushroom: Truffles. <em>Food Research International</em> 44: 2567–2581. https://doi.org/10.1016/j.foodres.2011.06.008</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Zhang, B.C. (1991) Revision of Chinese species of<em> Elaphomyces </em>(Ascomycotina, Elaphomycetales). <em>Mycological Research</em> 95 (8): 973–985. https://doi.org/10.1016/S0953-7562(09)80095-6</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Zhang, Z., Schwartz, S., Wagner, L. & Miller, W. (2000) A greedy algorithm for aligning DNA sequences. <em>Journal of Computational Biology </em>7: 203−214. https://doi.org/10.1089/10665270050081478</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Castellano, M.A., Trappe, J.M., Maser, Z. & Maser, S. (1986) <em>Key to spores of the genera of hypogeous fungi of North America, with reference to animal mycophagy.</em> Mad River Press, Eureka, 185 pp.</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Castellano, M.A., Guevara-Guerrero, G., García-Jiménez, J. & Trappe, J.M. (2012) <em>Elaphomyces appalachiensis</em> and <em>E</em>. <em>verruculosus</em> sp. nov. (Ascomycota Eurotiales, Elaphomycetaceae) from eastern North America.<em> Revista Mexicana de Micología</em> 35: 17−22.</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Castellano, M.A., Dentinger, B.T.M., Séné, O., Truong, C. & Henkel, T.W. (2016) New species of <em>Elaphomyces</em> (Elaphomycetaceae, Eurotiales, Ascomycota) from tropical rainforests of Cameroon and Guyana. <em>IMA Fungus</em> 7: 59–73. https://doi.org/10.5598/imafungus.2016.07.01.05</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Castellano, M.A., Elliott, T.F. & Trappe, J.M. (2018) Three new black <em>Elaphomyces</em> species (Elaphomycetaceae, Eurotiales, Ascomycota) from eastern North America with notes on selected European species.<em> Fungal Systematics and Evolution</em> 1 (1): 1−12. https://doi.org/10.3114/fuse.2018.01.01</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Castellano, M.A., Crabtree, C.D., Mitchell, D. & Healy, R.A. (2021) Eight new <em>Elaphomyces</em> species (Elaphomycetaceae, Eurotiales, Ascomycota) from eastern North America. <em>Fungal Systematics</em> <em>and Evolution</em> 7 (1): 113−131. https://doi.org/10.3114/fuse.2021.07.06</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Cázares, E., García, J., Castillo, J. & Trappe, J.M. (1992) Hypogeous Fungi from Northern Mexico. <em>Mycologia</em> 84 (3): 341–359. https://doi.org/10.2307/3760186</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Chauhan, O.P., Vijay, V., Pandey, A.K. & Semwal, A.D. (2021) Biochemical and Health Properties of Truffles. <em>Defence Life Science Journal </em>6 (3): 251–258. https://doi.org/10.14429/dlsj.6.15659</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">García-Romero, L., Guzmán, G. & Herrera, T. (1970) Especies de macromicetos citadas de México, I. Ascomycetes, Tremellales y Aphyllophorales. <em>Boletín de la Sociedad Mexicana de Micología </em>(4): 54–76. https://doi.org/10.33885/sf.1970.1.404</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Gómez-Reyes, V.M., Hernández-Salmerón, I.R., Terrón-Alfonso, A. & Guevara-Guerrero, G. (2012) Taxonomic study on <em>Elaphomyces</em> spp. (Ascomycota, Eurotiales, Elaphomycetaceae) from Michoacan, Mexico. <em>Revista Mexicana de Micología</em> 36: 57–82.</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Gómez-Reyes, V.M., Vázquez-Marrufo, G., Ortega Gómez, A.M. & Guevara Guerrero, G. (2018) Ascomicetos hipogeos de la región occidental del Sistema Volcánico Transversal, México.<em> Acta Botánica Mexicana</em> 125: 37–48. https://doi.org/10.21829/abm125.2018.1327</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Heim, R. (1957) Sur les psilocybes hallucinatoires des azteques et sur le microendémisme des agarics utilisées par les indiens du Mexique a des fins divinatoires. <em>Revue Mycologique </em>22 (3): 300–305. https://doi.org/10.3406/jatba.1956.2313</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Jeppson, M., Molia, A., Gustafsson, D. & Larsson, E. (2020) Fem för sverige nya hjorttryfflar (<em>Elaphomyces</em>). <em>Svensk Mykologisk Tidskrift</em> 41 (2): 2–11.</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Kornerup, A. & Wanscher, H. (1978) <em>Methuen handbook of colour.</em> 3rd edition. Eyre, London, 227 pp.</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Molia, A., Larsson, E., Jeppson, M., Læssøe, T. & Larsson, K-H. (2020) <em>Elaphomyces</em> section <em>Elaphomyces</em> (Eurotiales, Ascomycota)—taxonomy and phylogeny of North European taxa, with the introduction of three new species. <em>Fungal Systematic and Evolution</em> 5: 283–300. https://doi.org/10.3114/fuse.2020.05.14</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Paz, A., Bellanger, J.M., Lavoise, C., Molia, A., Ławrynowicz, M., Larsson, E., Ibarguen, I.O., Jeppson, M., Læssøe, T., Sauve, M., Richard, F. & Moreau, P.A. (2017) The genus <em>Elaphomyces</em> (Ascomycota, Eurotiales): a ribosomal DNA-based phylogeny and revised systematics of European ‘deer truffles’. <em>Persoonia</em> 38 (1): 197–239. https://doi.org/10.3767/003158517X697309</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Pegler, D.N., Spooner, B.M. & Young, T.W.K. (1993) <em>British truffles. A revision of British hypogeous fungi</em>. The Board of Trustees of the Royal Botanical Gardens, Kew, 215 pp.</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Pérez-Moreno, J. & Martínez-Reyes, M. (2014) Edible ectomycorrhizal mushrooms. Biofactories for sustainable development.<em> In: </em>Guevara-González, R. & Torres-Pacheco, I. (eds.) <em>Biosystems Engineering Biofactories for food production in the century XXI.</em> Springer, Berlin. pp. 151–233. https://doi.org/10.1007/978-3-319-03880-3_6</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Saltarelli, R., Ceccaroli, C.P., Barbieri, E. & Stocchi, V. (2008) Effect of storage on biochemical and microbiological parameters of edible truffle species. <em>Food Chemistry</em> 109: 8–16. https://doi.org/10.1016/j.foodchem.2007.11.075</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Shirakawa, M. & Tanaka, M. (2020) Two new deer truffle species, <em>Elaphomyces marmoratus</em> and <em>Elaphomyces fuscus</em> spp. nov., from a secondary forest in Japan. <em>Mycoscience</em> 61 (6): 315–322. https://doi.org/10.1016/j.myc.2020.07.002</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Trappe, J.M. & Guzmán, G. (1971) Notes on some hypogeous fungi from Mexico. <em>Mycologia</em> 63 (2): 317–332. https://doi.org/10.1080/00275514.1971.12019112</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Sukarno, N., Listiyowati, S., Rahayu, N. & Nara, K. (2019) <em>Elaphomyces tropicalis</em> sp. nov.: A new ectomycorrhizal fungus associated with dipterocarps from tropical Indonesia. <em>Mycoscience</em> 60 (2): 83–88. https://doi.org/10.1016/j.myc.2018.12.004</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Trappe, J.M., Guzmán, G. & Vázquez-Salinas, C. (1979) Observaciones sobre la identificación, distribución y usos de los hongos del género <em>Elaphomyces</em> en México. <em>Boletín de la Sociedad Mexicana de Micología</em> 13: 145–150.</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Vittadini, C. (1831) <em>Monographia tuberacearum. </em>Mediolani, 88 pp.</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">White, T.J., Bruns, T.D., Lee, S. & Taylor, J.W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics.<em> In: </em>Innis, M.A., Gelfand, D.H., Sninsky, J. & White, T.J. (Eds.) <em>PCR protocols: a guide to methods and applications.</em> Academic, San Diego, pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Wang, S. & Marcone, M.F. (2011) The biochemistry and biological properties of the world’s most expensive underground edible mushroom: Truffles. <em>Food Research International</em> 44: 2567–2581. https://doi.org/10.1016/j.foodres.2011.06.008</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Zhang, B.C. (1991) Revision of Chinese species of<em> Elaphomyces </em>(Ascomycotina, Elaphomycetales). <em>Mycological Research</em> 95 (8): 973–985. https://doi.org/10.1016/S0953-7562(09)80095-6</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Zhang, Z., Schwartz, S., Wagner, L. & Miller, W. (2000) A greedy algorithm for aligning DNA sequences. <em>Journal of Computational Biology </em>7: 203−214. https://doi.org/10.1089/10665270050081478</span></span></span></p>