Molecular phylogeny of Onobrychis sect. Onobrychis (Fabaceae-Hedysareae) with insights into its taxonomy and character evolution

ANAHITA HADADI; AKRAM KAVEH; HANYIEH NAFISI; SHAHROKH KAZEMPOUR-OSALOO

doi:10.11646/phytotaxa.592.3.2

Issue: Vol. 592 No. 3: 20 April 2023

Type: Article

Published: 2023-04-20

DOI: 10.11646/phytotaxa.592.3.2

Page range: 196-216

Abstract views: 197

PDF downloaded: 7

**Molecular phylogeny of Onobrychis sect. Onobrychis (Fabaceae-Hedysareae) with insights into its taxonomy and character evolution**

ANAHITA HADADI⁺⁻
AKRAM KAVEH⁺⁻
HANYIEH NAFISI⁺⁻
SHAHROKH KAZEMPOUR-OSALOO⁺⁻

Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran 14115-154, Iran

Phylogenetic analysis character evolution Onobrychis sect. Onobrychis monophyly subsection Eudicots

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Abstract

Onobrychis sect. Onobrychis, as the most species-rich section of the genus Onobrychis, ranges from Central Asia to Europe with a south extension into North Africa. In this study, we concentrated on the systematics of previously poorly represented O.sect. Onobrychis using a substantial taxon sampling. To test the monophyly of the section as well as to clarify interspecific relationships, the nuclear ribosomal DNA internal transcribed spacer(nrDNA ITS) and three chloroplast sequences (ndhF-rlp32, rpl32-trnL_(UAG), and trnG_(UCC)-trnS_(GCU)) were employed. Phylogenetic analyses were performed by maximum likelihood and Bayesian inferences. The results showed that O.sect. Onobrychis is monophyletic and composed of two main well-supported clades which are redefined as two subsections (O.subsect. Onobrychis, O. subsect. Macropterae).Ancestral character reconstruction of morphological traits: life form, wing to standard ratio, chromosome number, and ploidy level, indicated that short-winged petal is a synapomorphy for a group of species in the section while other traits have undergone parallel evolution. Possessing the basic chromosome number x= 7 has arisen twice, independently. It seems that perenniality and polyploidy are probably the key innovations in the diversification of O. sect. Onobrychis.

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