Abstract
Cephalaria saldaensis, collected from Eşeler Mountain around Salda Lake (Yeşilova-Burdur), one of Turkey’s important tourism regions, is described from South-West Anatolia as a new species. Diagnostic morphological and molecular characters from closely similar taxa are discussed. The geographical distribution of the new species and other related species is mapped. Taxonomic comments such as descriptive and diagnostic characters, distribution and ecology, conservation status for this new species are given in the current study. In this study, morphological characterization of three different Cephalaria spp. was also supported via molecular characterization based on PCR technology. Twenty-two ISSR primers were tested using a total of fifteen individuals belonging to three different Cephalaria spp. Five of these primers gave the most productive band profile. A total of 53 bands ranging from 250bp to 2000bp were obtained from the PCR products on agarose gel image, and the polymorphism calculated on the basis of the band profiles was 77.4%. The band profiles were also analyzed using the PopGen32 Software program. The constructed phylogenetic tree grouped the Cephalaria spp. individuals belonging to these three species into two main clusters and the phylogenetic analysis indicated that C. saldaensis was clearly different from the C. scoparia and C. dirmilensis.
References
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Aksoy, N., Göktürk, R.S., Açık, L. & Çelebi, A. (2007) <em>Cephalaria duzceёnsis</em> (Dipsacaceae), a new species from the western Black Sea Region, Turkey. <em>Nordic Journal of Botany </em>25: 64−69. https://doi.org/10.1111/j.0107-055X.2007.00100.x</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Brullo, S. & Erben, M. (2016) The genus <em>Limonium</em> (Plumbaginaceae) in Greece. <em>Phytotaxa</em> 240 (1): 1–212. https://doi.org/10.11646/phytotaxa.240.1.1</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Doyle, J.J. & Doyle, J.L. (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. <em>Phytochemical Bulletin</em> 19: 11–15.</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Düşen, O., Göktürk, R.S., Kaya, E., Sarpkaya, U. & Gürcan, B. (2018) Morphological and molecular determination of a new <em>Viola</em> species (Violaceae) from Turkey. <em>Phytotaxa</em> 369 (1): 37–46. https://doi.org/10.11646/phytotaxa.369.1.4</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Felix, F.C., das Chagas, K.P.T., dos Santos Ferrari, C., de Almeida Vieira, F. & Pacheco, M.V. (2020) Applications of ISSR markers in studies of genetic diversity of <em>Pityrocarpa moniliformis</em>. <em>Revista Caatinga</em> 33 (4): 1017–1024. https://doi.org/10.1590/1983-21252020v33n417rc</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Galatalı, S., Balcı, M.A., Akgüller, O. & Kaya, E. (2021) Production of Disease-Free Olive Seedlings with Artificial Intelligence and Biotechnological Methods. <em>European Journal of Biology and Biotechnology</em> 2 (3): 79–84. https://doi.org/10.24018/ejbio.2021.2.3.172</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Garmendia, A., Merle, H., Sanía, M., López, C. & Ferriol, M. (2022) Morphologic, genetic, and biogeographic continua among subspecies hinder the conservation of threatened taxa: the case of <em>Centaurea aspera</em> ssp. <em>scorpiurifolia </em>(Asteraceae). <em>Scientific Reports</em> 12: n. 932. https://doi.org/10.1038/s41598-022-04934-4</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Göktürk, R.S. & Sümbül, H. (1997) A new species of <em>Cephalaria</em> (Dipsacaceae) from South Anatolia, Turkey. <em>Annales Botanici Fennici</em> 34: 153−155. </span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Göktürk, R.S. & Sümbül, H. (2003)<em> Cephalaria aytachii </em>(Dipsacaceae), a new species from Central Anatolia, Turkey. <em>Annales Botanici Fennici</em> 40: 123−127.</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Göktürk, R.S. & Sümbül, H. (2014) A taxonomic revision of the genus <em>Cephalaria </em>(Caprifoliaceae) in Turkey. <em>Turkish Journal of Botany</em> 38: 927−968. https://doi.org/10.3906/bot.1310-6</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Göktürk, R.S. & Sümbül, H. (2016) <em>Cephalaria anamurensis</em> (Caprifoliaceae), a new species from south Anatolia, Turkey. <em>PhytoKeys</em> 65: 25−33. https://doi.org/10.3897/phytokeys.65.8571</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Göktürk, R.S., Düşen, O., Kaya, E., Gürcan, B. & Sarpkaya, U. (2019) A new variety of <em>Plocama calabrica</em> (Rubiaceae) from Denizli (Turkey) confirmed by morphological and molecular ISSR markers. <em>Acta Botanica Croatica</em> 78 (2): 142–146. https://doi.org/10.2478/botcro-2019-0016 </span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Göktürk, R.S., Sümbül, H. & Açık, L. (2003) A new species of <em>Cephalaria</em> Schrad. ex Roem. & Schult. (Dipsacaceae), including a new variety from East Anatolia, Turkey. <em>Israel Journal of Plant Sciences</em> 51: 59−65. https://doi.org/10.1560/332B-JGEF-R6RC-DHL5</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Göktürk, R.S., Sümbül, H., Çelebi, A. & Açık, L. (2012) Two new species of<em> Cephalaria </em>(Caprifoliaceae) from Turkey. <em>Turkish Journal of Botany</em> 36: 311−321. https://doi.org/10.3906/bot-1101-4 </span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Gwinner, R., Setotaw, T.A., Rodrigues, F.A., França, D.V.C., da Silveira, F.A., Pio, L.A.S. & Pasqual, M. (2016) Population structure of <em>Annona crassiflora</em>: an endemic plant species of the Brazilian Cerrado. <em>Genetics and Molecular Research</em> 15 (4): gmr15049137. https://doi.org/10.4238/gmr15049137 </span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">IUCN Standards and Petitions Committee (2019) <em>Guidelines for Using the IUCN Red List Categories and Criteria. Version 14. Prepared by the Standards and Petitions Committee</em>. Gland, Switzerland and Cambridge, UK,16 pp.</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Kaya, E. & Souza, F.V.D. (2017) Comparison of two PVS2-based procedures for cryopreservation of commercial sugarcane (<em>Saccharum</em> spp.) germplasm and confirmation of genetic stability after cryopreservation using ISSR markers. <em>In Vitro Cellular & Developmental Biology–Plant</em> 53: 410–417. https://doi.org/10.1007/s11627-017-9837-2 </span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Kaya, E. & Yılmaz Gökdoğan, E. (2016) Using two retrotransposon based marker systems (IRAP and REMAP) for molecular characterization of olive (<em>Olea europaea</em> L.) cultivars. <em>Notulae Botanicae Horti Agrobotanici Cluj-Napoca</em> 44 (1): 167–174. https://doi.org/10.15835/nbha44110158 </span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Kaya, E. (2015) ISSR Analysis for determination of genetic diversity and relationship in eight Turkish olive (<em>Olea europaea</em> L.) cultivars. <em>Notulae Botanicae Horti Agrobotanici Cluj-Napoca</em> 2015, 43 (1): 96–99. https://doi.org/10.15835/nbha4319818 </span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Kuş, S. & Göktürk, R.S. (2005) A new <em>Cephalaria</em> species (Dipsacaceae) from the European part of Turkey. <em>Nordic Journal of Botany</em> 23: 427−430. https://doi.org/10.1111/j.1756-1051.2003.tb00415.x</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Martins-Lopes, P., Lima-Brito, J., Gomes, S., Meirinhos, J., Santos, L. & Guedes-Pinto, H. (2007) RAPD and ISSR molecular markers in <em>Olea europaea</em> L.: Genetic variability and molecular cultivar identification. <em>Genetic Resources and Crop Evolution</em>, 54:117–128. https://doi.org/10.1007/s10722-005-2640-7 </span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Matthews, V.A. (1972) <em>Cephalaria</em> Schrad. ex Roem. & Schult. <em>In</em>: Davis, P.H. (Ed.) <em>Flora of Turkey and the east Aegean islands,</em> <em>vol. 4.</em> Edinburgh University Press, Edinburgh, pp. 585−597.</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Matthews, V.A. (1988) <em>Cephalaria</em> Schrad. ex Roem. & Schult. <em>In</em>: Davis, P.H., Mill, R.R. & Tan, K. (Eds.) <em>Flora of Turkey and the east Aegean islands,</em> <em>vol.10</em>, suppl. Edinburgh University Press, Edinburgh, pp. 156.</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Özüdoğru, E.A., Kaya, E., Kirdok, E. & Issever-Öztürk, S. (2011) <em>In vitro</em> propagation from young and mature explants of thyme (<em>Thymus vulgaris</em> and <em>T. longicaulis</em>) resulting in genetically stable shoots. <em>In Vitro Cellular & Developmental Biology–Plant</em> 47: 309–320. https://doi.org/10.1007/s10722-005-2640-7 </span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Pakseresht, F., Talebi, R. & Karami, E. (2013) Comparative assessment of ISSR, DAMD and SCoT markers for evaluation of genetic diversity and conservation of landrace chickpea (<em>Cicer arietinum</em> L.) genotypes collected from north-west of Iran. <em>Physiology and Molecular Biology of Plants</em> 19 (4): 563–74. https://doi.org/10.1007/s12298-013-0181-7</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Parolly, G. & Eren, Ö. (2007) Contributions to the flora of Turkey, 2. <em>Willdenowia</em> 37: 243−271. https://doi.org/10.3372/wi.37.37114</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Ranjbar, M. & Ranjbar, Z. (2018) A contribution to the taxonomy of <em>Cephalaria</em> subgen. <em>Cephalaria</em> (Caprifoliaceae) in eastern Turkey. <em>Feddes Repertorium</em> 129: 13–24. https://doi.org/10.1002/fedr.201700004</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Reddy, M., Sarla, N. & Siddiq, E. (2002) Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. <em>Euphytica</em> 128: 9–17. https://doi.org/10.1023/A:1020691618797</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Reveal, J.L. & Chase, M.W. (2011) APG III: Bibliographical information and synonymy of Magnoliidae. <em>Phytotaxa</em> 19: 71−134. https://doi.org/10.11646/phytotaxa.19.1.4</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Smıkal, P., Bačová-Kerteszová, N., Kalendar, R., Corander, J., Schulman, A.H. & Pavelek, M. (2011) Genetic diversity of cultivated flax (<em>Linum usitatissimum</em> L.) germplasm assessed by retrotransposon-based markers. <em>Theoretical and Applied Genetics</em> 122: 1385–1397. https://doi.org/10.1007/s00122-011-1539-2 </span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Sümbül, H. (1991) Ten new species from Anatolia and two new records for the flora of Turkey. <em>Edinburgh Journal of Botany</em> 48: 27−40. https://doi.org/10.1017/S0960428600003577</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Szabó, Z. (1940)<em> Cephalaria-génusz monográfiája</em>. Kiadja A Magyar Tudományos Akadémia Press, Budapest, Hungary, 352 pp.</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Tanksley, S.D., Young, N.D., Peterson, A.H. & Bonierbale, M.W. (1989) RFLP mapping in plant breeding: new tools for old sciences. <em>Biotechnology</em> 7: 257–264. https://doi.org/10.1038/nbt0389-257</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Thiers, B. (2023) Index Herbariorum: A global directory of public herbaria and associated staff. New York Botanical Garden’s Virtual Herbarium. Available from: https://sweetgum.nybg.org/science/ih/ (accessed 18 January 2023)</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Üstüner, H., Yavuz, M., Göktürk, R.S., Kaya, E. & Galatalı, S. (2022) Morphological and molecular notes of <em>Gypsophila pilulifera</em>. <em>Phytotaxa</em> 369 (1): 99–118. https://doi.org/10.11646/phytotaxa.556.2.1</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Wagensommer, R.P. & Venanzoni, R. (2021) <em>Geranium lucarinii </em>sp. nov. and re-evaluation of<em> G. kikianum </em>(Geraniaceae). <em>Phytotaxa</em> 489 (3): 252–262. https://doi.org/10.11646/phytotaxa.489.3.2</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Yıldırımlı, Ş. (2019) Two new species of <em>Cephalaria</em> (Dipsacaceae/Caprifoliaceae) from Turkey. <em>The Herb Journal of Systematic Botany</em> 26 (2): 105–111.</span></span></span></p>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Yuan, C.Y., Wang, P., Chen, P.P., Xiao, W.J., Zhang, C., Hu, S., Zhou, P., Chang, H.P., He, Z., Hu, R., Lu, X.T., Ye, J.Z. & Guo, X.H. (2015) Genetic diversity revealed by morphological traits and ISSR markers in 48 Okras (<em>Abelmoschus escullentus</em> L.). <em>Physiology and Molecular Biology of Plants</em> 21 (3): 359–364. https://doi.org/10.1007/s12298-015-0303-5</span></span></span></p>