Abstract
Hedysarum yilmazunalii Kandemir, F. Yıldız & H.İ. Turkoğlu is described as a new species from Turkey (Eastern Anatolia). The distribution range of the species is restricted to Kemah and Kemaliye districts in Erzincan province on serpentine soils. Hedysarum yilmazunalii is close to Hedysarum candidissimum Freyn in terms of morphological and molecular data. It is also morphologically close to Hedysarum malatyanum Govaerts. In this study, the morphological comparison of the new species with related species is presented. Molecular studies are also done using nrDNA ITS and cpDNA trnL-F regions. ITS data gives better results in phylogenetic analysis. In addition to the morphological data, the results of DNA analysis of the new species are also discussed.
References
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Ahangarian, S., Kazempour Osaloo, S. & Maassoumi, A.A. (2007) Molecular phylogeny of the tribe Hedysareae with special reference to Onobrychis (Fabaceae) as inferred from nrDNA ITS sequences. Iran Journal of Botany 13: 64–74.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Chaghamirzaei, E., Ghahremaninejad, F. & Assadi, M. (2022) Hedysarum pseudomacranthum (Fabaceae), a new species from Talesh Mountains, northwestern Iran. The Iranian Journal of Botany 28 (1): 1–5.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Choi, B.H. & Ohashi, H. (2003) Generic criteria and an infrageneric system for Hedysarum and related genera (Papilionoideae—Leguminosae). Taxon 52 (3): 567–576. https://doi.org/10.2307/3647455</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Cuttelod, A., García, N., Malak, D.A., Temple, H.J. & Katariya, V. (2009) The Mediterranean: a biodiversity hotspot under threat. Wildlife in a Changing World–an analysis of the 2008 IUCN Red List of Threatened Species 89: 9.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Ekim, T., Koyuncu, M., Vural, M., Duman, H., Aytaç, Z. & Ad?güzel, N. (2000) Türkiye Bitkileri K?rm?z? Kitab? (E?relti ve Tohumlu Bitkiler) / Red Data Book of Turkish Plants (Pteridophyta and Spermatophyta). Turkish Association for the Conservation of Nature, and Van 100. Year University Press., Ankara.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Felsenstein, J. (1985) Confidence limits on the phylogenies: an approach using the bootstrap. Evolution 39: 783–791. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Hall, T.A. (1999) Bioedit: a user-friendly biological sequence alignment editor and analyses program for Windows 95/98/NT. Nucleic Acids symposium 41: 95–98.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Hamzao?lu, E. & Koc, M. (2020) Hedysarum turcicum (Hedysareae, Fabaceae), a new species from Turkey. Phytotaxa 428 (1): 1–6. https://doi.org/10.11646/phytotaxa.428.1.1</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Hedge, I.C. (1970) Hedysarum L. In: Davis, P.D. (Ed.) Flora of Turkey and East Aegean Islands, vol 3. Edinburgh University Press, Edinburgh, pp. 549–560.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Médail, F. & Diadema, K. (2009) Glacial refugia influence plant diversity patterns in the Mediterranean Basin. Journal of biogeography, 36 (7): 1333–1345. https://doi.org/10.1111/j.1365-2699.2008.02051.x</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Menemen, Y., Aytaç, Z. & Kandemir, A. (2016) Türkçe Bilimsel Bitki Adlar? Yönergesi. Ba?bahçe Bilim Dergisi 3 (3): 1–3.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Nafisi, H., Kazempour-Osaloo, S., Mozaffarian, V. & Schneeweiss, G.M. (2019) Molecular phylogeny and divergence times of the genus Hedysarum (Fabaceae) with special reference to section Multicaulia in Southwest Asia. Plant Systematics and Evolution 305: 1001–1017. https://doi.org/10.1007/s00606-019-01620-3</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Perrino, E.V. (2011) New data on Aegilops uniaristata Vis. endangered taxon in Italy. Natura Croatica 20 (1): 117–123.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Perrino, E.V. & Signorile, G. (2009) Costa di Monopoli (Puglia): check-list della flora vascolare. Informatore Botanico Italiano 41 (2): 263–279.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Perrino, E.V., Signorile, G. & Marvulli, M. (2013a) A first checklist of the vascular flora of the “Polignano a Mare” coast (Apulia, southern Italy). Natura Croatica 22 (2): 295–318.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Perrino E.V. & Wagensommer R.P. (2012) Updates on plant species of Gargano (Apulia region) related to the 92/43/EEC Directive Habitats (Aggiornamenti floristici per il Gargano (Puglia) con riferimento agli habitat della Direttiva 92/43/EEC). Informatore Botanico Italiano 44 (1): 163–170.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Perrino, E.V., Wagensommer, R.P., Silletti, G.N., Signorile, G. & Angiulli, F. (2013b) Nuovi dati distributivi e relazione con la Direttiva 92/43/CEE di taxa critici pugliesi dalla Provincia di Bari. Informatore Botanico Italiano 45 (1): 53–62.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Perrino, E.V., Tomaselli, V., Wagensommer, R.P., Silletti, G.N., Esposito, A. & Stinca, A. (2022) Ophioglossum lusitanicum L.: New Records of Plant Community and 92/43/EEC Habitat in Italy. Agronomy 2022, 12, 3188. https://doi.org/10.3390/agronomy12123188</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Polhill, R.M. (1981) Papilionoideae. In: Polhill, R.M. & Raven, P.H. (Eds.) Advances in Legume Systematics. Part 1. Royal Botanic Gardens, Kew, UK, pp. 367–370.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Stanford, A.M., Harden, R. & Parks, C.R. (2000) Phylogeny and biogeography of Juglans (Juglandaceae) based on matK and ITS sequence data. American Journal of Botany 87 (6):872–882. https://doi.org/10.2307/2656895</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Taberlet, P., Gielly, L., Pautou, G. & Bouvet, J. (1991) Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Molecular Biology 17: 1105–1109. https://doi.org/10.1007/BF00037152</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30 (12): 2725–2729. https://doi.org/10.1093/molbev/mst197</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Wagensommer, R.P., Bartolucci, F., Fiorentino, M., Licht, W., Peccenini, S., Perrino, E.V. & Venanzoni, R. (2017) First record for the flora of Italy and lectotypification of the name Linum elegans (Linaceae). Phytotaxa 296 (2): 161–170. https://doi.org/10.11646/phytotaxa.296.2.5</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Wagensommer, R.P., Fröhlich, T. & Fröhlich, M. (2014a) First record of the southeast European species Cerinthe retorta Sibth. & Sm. (Boraginaceae) in Italy and considerations on its distribution and conservation status. Acta Bot. Gallica: Botany Letters 161 (2): 111–115. https://doi.org/10.1080/12538078.2014.892438</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Wagensommer, R.P., Perrino, E.V. & Silletti, G.N. (2014b) Carex phyllostachys C. A. Mey. (Cyperaceae) New for Italy and Phytogeographical Considerations. Phyton 54 (2): 215–222. https://doi.org/10.12905/0380.phyton54(2)2014-0215</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Wh?te, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplifications and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M., Gelfand, D., Sninsky, J. & White, T. (Eds.) PCR protocols: a guide to methods and applications. Academic Press, San Diego, California, USA, pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Y?ld?z, B., C?plak, B. & Aktoklu, E. (1999) Fruit morphology of sections of the genus Onobrychis Miller (Fabaceae) and its phylogenetic implications. Israel Journal of Plant Sciences 47: 269–282. https://doi.org/10.1080/07929978.1999.10676784</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Yurkevich, O.Y., Samatadze, T.E., Selyutina, I.Y., Romashkina, S.I., Zoshchuk, S.A., Amosova, A.V. & Muravenko, O.V. (2021) Molecular Cytogenetics of Eurasian Species of the Genus Hedysarum L. (Fabaceae). Plants 10 (1): 89. https://doi.org/10.3390/plants10010089</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Chaghamirzaei, E., Ghahremaninejad, F. & Assadi, M. (2022) Hedysarum pseudomacranthum (Fabaceae), a new species from Talesh Mountains, northwestern Iran. The Iranian Journal of Botany 28 (1): 1–5.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Choi, B.H. & Ohashi, H. (2003) Generic criteria and an infrageneric system for Hedysarum and related genera (Papilionoideae—Leguminosae). Taxon 52 (3): 567–576. https://doi.org/10.2307/3647455</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Cuttelod, A., García, N., Malak, D.A., Temple, H.J. & Katariya, V. (2009) The Mediterranean: a biodiversity hotspot under threat. Wildlife in a Changing World–an analysis of the 2008 IUCN Red List of Threatened Species 89: 9.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Ekim, T., Koyuncu, M., Vural, M., Duman, H., Aytaç, Z. & Ad?güzel, N. (2000) Türkiye Bitkileri K?rm?z? Kitab? (E?relti ve Tohumlu Bitkiler) / Red Data Book of Turkish Plants (Pteridophyta and Spermatophyta). Turkish Association for the Conservation of Nature, and Van 100. Year University Press., Ankara.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Felsenstein, J. (1985) Confidence limits on the phylogenies: an approach using the bootstrap. Evolution 39: 783–791. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Hall, T.A. (1999) Bioedit: a user-friendly biological sequence alignment editor and analyses program for Windows 95/98/NT. Nucleic Acids symposium 41: 95–98.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Hamzao?lu, E. & Koc, M. (2020) Hedysarum turcicum (Hedysareae, Fabaceae), a new species from Turkey. Phytotaxa 428 (1): 1–6. https://doi.org/10.11646/phytotaxa.428.1.1</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Hedge, I.C. (1970) Hedysarum L. In: Davis, P.D. (Ed.) Flora of Turkey and East Aegean Islands, vol 3. Edinburgh University Press, Edinburgh, pp. 549–560.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Médail, F. & Diadema, K. (2009) Glacial refugia influence plant diversity patterns in the Mediterranean Basin. Journal of biogeography, 36 (7): 1333–1345. https://doi.org/10.1111/j.1365-2699.2008.02051.x</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Menemen, Y., Aytaç, Z. & Kandemir, A. (2016) Türkçe Bilimsel Bitki Adlar? Yönergesi. Ba?bahçe Bilim Dergisi 3 (3): 1–3.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Nafisi, H., Kazempour-Osaloo, S., Mozaffarian, V. & Schneeweiss, G.M. (2019) Molecular phylogeny and divergence times of the genus Hedysarum (Fabaceae) with special reference to section Multicaulia in Southwest Asia. Plant Systematics and Evolution 305: 1001–1017. https://doi.org/10.1007/s00606-019-01620-3</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Perrino, E.V. (2011) New data on Aegilops uniaristata Vis. endangered taxon in Italy. Natura Croatica 20 (1): 117–123.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Perrino, E.V. & Signorile, G. (2009) Costa di Monopoli (Puglia): check-list della flora vascolare. Informatore Botanico Italiano 41 (2): 263–279.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Perrino, E.V., Signorile, G. & Marvulli, M. (2013a) A first checklist of the vascular flora of the “Polignano a Mare” coast (Apulia, southern Italy). Natura Croatica 22 (2): 295–318.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Perrino E.V. & Wagensommer R.P. (2012) Updates on plant species of Gargano (Apulia region) related to the 92/43/EEC Directive Habitats (Aggiornamenti floristici per il Gargano (Puglia) con riferimento agli habitat della Direttiva 92/43/EEC). Informatore Botanico Italiano 44 (1): 163–170.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Perrino, E.V., Wagensommer, R.P., Silletti, G.N., Signorile, G. & Angiulli, F. (2013b) Nuovi dati distributivi e relazione con la Direttiva 92/43/CEE di taxa critici pugliesi dalla Provincia di Bari. Informatore Botanico Italiano 45 (1): 53–62.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Perrino, E.V., Tomaselli, V., Wagensommer, R.P., Silletti, G.N., Esposito, A. & Stinca, A. (2022) Ophioglossum lusitanicum L.: New Records of Plant Community and 92/43/EEC Habitat in Italy. Agronomy 2022, 12, 3188. https://doi.org/10.3390/agronomy12123188</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Polhill, R.M. (1981) Papilionoideae. In: Polhill, R.M. & Raven, P.H. (Eds.) Advances in Legume Systematics. Part 1. Royal Botanic Gardens, Kew, UK, pp. 367–370.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Stanford, A.M., Harden, R. & Parks, C.R. (2000) Phylogeny and biogeography of Juglans (Juglandaceae) based on matK and ITS sequence data. American Journal of Botany 87 (6):872–882. https://doi.org/10.2307/2656895</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Taberlet, P., Gielly, L., Pautou, G. & Bouvet, J. (1991) Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Molecular Biology 17: 1105–1109. https://doi.org/10.1007/BF00037152</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30 (12): 2725–2729. https://doi.org/10.1093/molbev/mst197</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Wagensommer, R.P., Bartolucci, F., Fiorentino, M., Licht, W., Peccenini, S., Perrino, E.V. & Venanzoni, R. (2017) First record for the flora of Italy and lectotypification of the name Linum elegans (Linaceae). Phytotaxa 296 (2): 161–170. https://doi.org/10.11646/phytotaxa.296.2.5</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Wagensommer, R.P., Fröhlich, T. & Fröhlich, M. (2014a) First record of the southeast European species Cerinthe retorta Sibth. & Sm. (Boraginaceae) in Italy and considerations on its distribution and conservation status. Acta Bot. Gallica: Botany Letters 161 (2): 111–115. https://doi.org/10.1080/12538078.2014.892438</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Wagensommer, R.P., Perrino, E.V. & Silletti, G.N. (2014b) Carex phyllostachys C. A. Mey. (Cyperaceae) New for Italy and Phytogeographical Considerations. Phyton 54 (2): 215–222. https://doi.org/10.12905/0380.phyton54(2)2014-0215</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Wh?te, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplifications and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M., Gelfand, D., Sninsky, J. & White, T. (Eds.) PCR protocols: a guide to methods and applications. Academic Press, San Diego, California, USA, pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Y?ld?z, B., C?plak, B. & Aktoklu, E. (1999) Fruit morphology of sections of the genus Onobrychis Miller (Fabaceae) and its phylogenetic implications. Israel Journal of Plant Sciences 47: 269–282. https://doi.org/10.1080/07929978.1999.10676784</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Yurkevich, O.Y., Samatadze, T.E., Selyutina, I.Y., Romashkina, S.I., Zoshchuk, S.A., Amosova, A.V. & Muravenko, O.V. (2021) Molecular Cytogenetics of Eurasian Species of the Genus Hedysarum L. (Fabaceae). Plants 10 (1): 89. https://doi.org/10.3390/plants10010089</span></span></span>