Abstract
A wood-inhabiting fungal species, Physisporinus yunnanensis is proposed based on a combination of morphological features and molecular evidence. Physisporinus yunnanensis is characterized by annual, resupinate, bone-hard basidiomata with an olivaceous to black pore surface, a monomitic hyphal system, presence of hyphoid cystidia at dissepiment edge and subglobose, smooth basidiospores measuring as 4–5.5 × 3.5–5 µm, and causing a white rot. The phylogenetic analyses based on molecular data of ITS+nLSU sequences showed that P. yunnanensis is sister to P. furcatus with high support (100% BS, 100% BP, 1.00 BPP). Both morphological characteristics and molecular phylogenetic analyses confirmed the placement of the new species in Physisporinus.
References
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<p>Ryvarden, L. & Johansen, I. (1980) <em>A preliminary polypore flora of East Africa</em>. Oslo, Fungiflora, Norway, 636 pp.</p>
<p>Ryvarden, L. & Melo, I. (2014) Poroid fungi of Europe<em>.</em> <em>Synopsis Fungorum</em> 31: 1–455.</p>
<p>Ryvarden, L. (1972) A critical checklist of the Polyporaceae in tropical East Africa. <em>Norwegian Journal of Botany</em> 19: 229–238.</p>
<p>Ryvarden, L. & Gilbertson, R.L. (1994) European polypores. Part 2. <em>Synopsis Fungorum</em> 7: 394–743.</p>
<p>Ryvarden, L. (2016) Neotropical polypores part 3. Polyporaceae,<em> Obba</em>-<em>Wrightoporia</em>. <em>Synopsis Fungorum</em> 46: 445–613.</p>
<p>Swofford, D.L. (2002) PAUP*: phylogenetic analysis using parsimony (*and other methods). Version 4.0b10. <em>Sinauer Associates, Massachusetts</em>.</p>
<p>Vampola, P. & Vlasák, J. (2012) <em>Rigidoporus pouzarii</em>, a new polypore species related to <em>Rigidoporus crocatus</em>. <em>Czech Mycology</em> 64: 3–11.</p>
<p>Wang, C.G. & Dai, Y.C. (2022) Phylogeny and taxonomy of <em>Spongipellis</em> (Polyporales, Basidiomycota) and its micromorphological similar genera. <em>Mycological Progress</em> 21: 1–18. https://doi.org/10.1007/s11557-022-01817-w</p>
<p>White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. <em>PCR protocols: a guide to methods and applications</em> 18: 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1</p>
<p>Wu, F., Chen, J.J., Ji, X.H., Vlasák, J. & Dai, Y.C. (2017) Phylogeny and diversity of the morphologically similar polypore genera <em>Rigidoporus</em>, <em>Physisporinus</em>, <em>Oxyporus</em>, and <em>Leucophellinus</em>. <em>Mycologia</em> 109: 749–765. https://doi.org/10.1080/00275514.2017.1405215</p>
<p>Zhao, C.L., Cui, B.K. & Dai, Y.C. (2014) Morphological and molecular identifification of two new species of <em>Hyphodontia</em> (Schizoporaceae, Hymenochaetales) from Southern China. Cryptogam. <em>Mycologie</em> 35: 87–97. https://doi.org/10.7872/crym.v35.iss1.2014.87</p>
<p>Zhao, C.L. & Wu, Z.Q. (2017) <em>Ceriporiopsis kunmingensis</em> sp. nov. (Polyporales, Basidiomycota) evidenced by morphological characters and phylogenetic analysis. <em>Mycological Progress </em>16: 93–100. https://doi.org/10.1007/s11557-016-1259-8</p>
<p>Zhou, M., Dai, Y.C., Vlasák, J. & Yuan, Y. (2021) Molecular phylogeny and global diversity of the genus <em>Haploporus</em> (Polyporales, Basidiomycota). <em>Journal of Fungi</em> 7: 96. https://doi.org/10.3390/jof7020096</p>
<p>Chen, J.J. & Dai, Y.C. (2021) Two new species of <em>Physisporinus</em> (Polyporales, Basidiomycota) from Yunnan, Southwest China. <em>Mycological Progress</em> 20: 1–10. https://doi.org/10.1007/s11557-020-01647-8.</p>
<p>Dai, S.J. & Dai, Y.C. (2018) Morphological characters and molecular data reveal a new species of <em>Physisporinus</em> (Basidiomycota) from Southeast Asia. <em>Mycosystema</em> 37: 145–150. https://doi.org/10. 13346/j.mycosystema.170210</p>
<p>Dai, Y.C. (1998) Changbai wood-rotting fungi 9. Three new species and other species in <em>Rigidoporus</em>, <em>Skeletocutis</em> and <em>Wolfiporia</em> (Basidiomycota, Aphyllophorales). <em>Annales Botanici Fennici</em> 35: 143–154.</p>
<p>Decock, C. & Ryvarden, L. (2021) Aphyllophorales of Africa 48 some poroid species from São Tomé. <em>Synopsis Fungorum</em> 44: 14–18.</p>
<p>Felsenstein, J. (1985) Confidence intervals on phylogenetics: An approach using bootstrap. <em>Evolution</em> 39: 783–791. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x</p>
<p>Fisher, A.B. & Fong, S.S. (2014) Lignin biodegradation and industrial implications. <em>AIMS Bioengineering</em> 1: 92–112. https://doi.org/10.3934/bioeng.2014.2.92</p>
<p>Gilbertson, R.L. & Ryvarden, L. (1987) <em>North American Polypores</em> Vol. 2. Megasporoporia-Wrightoporia. pp. 437–885.</p>
<p>Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. <em>Nucleic Acids Symposium Series</em> 41: 95–98.</p>
<p>Hjortstam, K. & Ryvarden, L. (1980) Studies in tropical Corticiaceae (Basidiomycetes). II. <em>Mycotaxon</em> 12: 168–184.</p>
<p>Ipulet, P. & Ryvarden, L. (2005) New and interesting polypores from Uganda. <em>Synopsis Fungorum</em> 20: 87–99.</p>
<p>Karsten, P.A. (1889) Kritisk öfversigt af Finlands Basidsvampar (Basidiomycetes; Gastero- & Hymenomycetes). <em>Bidrag till Kännedom av Finlands Natur och Folk</em> 48: 1–470.</p>
<p>Marcot, B.G. (2017) A review of the role of fungi in wood decay of forest ecosystems. <em>Res. Note PNW-RN-575.Portland, OR: US Department of Agriculture, Forest Service, Pacific Northwest Research Station </em>575: 1–31.</p>
<p>Miettinen, O., Larsson, E., Sjokvist, E. & Larsson, K.H. (2012) Comprehensive taxon sampling reveals unaccounted diversity and morphological plasticity in a group of dimitic polypores (Polyporales, Basidiomycota). <em>Cladistics</em> 28: 251–270. https://doi.org/10.1111/j. 1096-0031.2011.00380.x</p>
<p>Miller, M.A., Pfeiffer, W. & Schwartz, T. (2012) The CIPRES science gateway: enabling high-impact science for phylogenetics researchers with limited resources. <em>In Proceedings of the 1st Conference of the Extreme Science and Engineering Discovery Environment</em>: <em>Bridging from the extreme to the campus and beyond.</em> pp. 1–8. https://doi.org/10.1145/2335755.2335836</p>
<p>Nakasone, K.K., Ortiz-Santana, B. & He, S.H. (2021) Taxonomic studies of crust fungi with spines in <em>Radulomyces</em>, <em>Sarcodontia</em>, and the new genus <em>Noblesia</em>. <em>Mycological Progress</em> 20: 1479–1501. https://doi.org/10.1007/s11557-021-01746-0</p>
<p>Niemelä T. (2005) Polypores, lignicolous fungi. <em>Norrlinia</em> 13: 1–320.</p>
<p>Núñez, M., Parmasto, E. & Ryvarden, L. (2001) New and interesting polypores from East Russia. <em>Fungal Diversity</em> 6: 107–114.</p>
<p>Núñez, M. & Ryvarden, L. (1999) New and interesting polypores from Japan. <em>Fungal Diversity</em> 3: 107–121.</p>
<p>Nylander, J.A.A. (2004) MrModeltest v.2. Program Distributed by the Author. Evolutionary Biology Centre, Uppsala University: Uppsala, Sweden.</p>
<p>Petersen, J.H. (1996) Farvekort. <em>The Danish Mycological Society’s Colour-Chart</em>. Foreningen til Svampekundskabens Fremme, Greve, Germany, pp. 1–6.</p>
<p>Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. <em>Systematic biology</em> 61: 539–542. https://doi.org/10.1093/sysbio/sys029</p>
<p>Ryvarden, L. & Johansen, I. (1980) <em>A preliminary polypore flora of East Africa</em>. Oslo, Fungiflora, Norway, 636 pp.</p>
<p>Ryvarden, L. & Melo, I. (2014) Poroid fungi of Europe<em>.</em> <em>Synopsis Fungorum</em> 31: 1–455.</p>
<p>Ryvarden, L. (1972) A critical checklist of the Polyporaceae in tropical East Africa. <em>Norwegian Journal of Botany</em> 19: 229–238.</p>
<p>Ryvarden, L. & Gilbertson, R.L. (1994) European polypores. Part 2. <em>Synopsis Fungorum</em> 7: 394–743.</p>
<p>Ryvarden, L. (2016) Neotropical polypores part 3. Polyporaceae,<em> Obba</em>-<em>Wrightoporia</em>. <em>Synopsis Fungorum</em> 46: 445–613.</p>
<p>Swofford, D.L. (2002) PAUP*: phylogenetic analysis using parsimony (*and other methods). Version 4.0b10. <em>Sinauer Associates, Massachusetts</em>.</p>
<p>Vampola, P. & Vlasák, J. (2012) <em>Rigidoporus pouzarii</em>, a new polypore species related to <em>Rigidoporus crocatus</em>. <em>Czech Mycology</em> 64: 3–11.</p>
<p>Wang, C.G. & Dai, Y.C. (2022) Phylogeny and taxonomy of <em>Spongipellis</em> (Polyporales, Basidiomycota) and its micromorphological similar genera. <em>Mycological Progress</em> 21: 1–18. https://doi.org/10.1007/s11557-022-01817-w</p>
<p>White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. <em>PCR protocols: a guide to methods and applications</em> 18: 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1</p>
<p>Wu, F., Chen, J.J., Ji, X.H., Vlasák, J. & Dai, Y.C. (2017) Phylogeny and diversity of the morphologically similar polypore genera <em>Rigidoporus</em>, <em>Physisporinus</em>, <em>Oxyporus</em>, and <em>Leucophellinus</em>. <em>Mycologia</em> 109: 749–765. https://doi.org/10.1080/00275514.2017.1405215</p>
<p>Zhao, C.L., Cui, B.K. & Dai, Y.C. (2014) Morphological and molecular identifification of two new species of <em>Hyphodontia</em> (Schizoporaceae, Hymenochaetales) from Southern China. Cryptogam. <em>Mycologie</em> 35: 87–97. https://doi.org/10.7872/crym.v35.iss1.2014.87</p>
<p>Zhao, C.L. & Wu, Z.Q. (2017) <em>Ceriporiopsis kunmingensis</em> sp. nov. (Polyporales, Basidiomycota) evidenced by morphological characters and phylogenetic analysis. <em>Mycological Progress </em>16: 93–100. https://doi.org/10.1007/s11557-016-1259-8</p>
<p>Zhou, M., Dai, Y.C., Vlasák, J. & Yuan, Y. (2021) Molecular phylogeny and global diversity of the genus <em>Haploporus</em> (Polyporales, Basidiomycota). <em>Journal of Fungi</em> 7: 96. https://doi.org/10.3390/jof7020096</p>