Abstract
Phyllachora species are biotrophic, obligate plant parasitic fungi featuring a high degree of host specificity, a high degree of species richness, and worldwide distribution. In this study, two Phyllachora species were collected from different hosts in Yunnan province, China. Based on morphological characteristics and multigene (LSU, ITS, SSU) phylogenetic analyses, they are introduced as two new taxa. Photographs, detailed descriptions, and notes are provided.
References
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Berbee, M.L. & Taylor, J.W. (1992) Detecting morphological convergence in true fungi using 18S rRNA gene sequence data. Biosystems 28: 117–125. https://doi.org/10.1016/0303-2647(92)90014-p</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Cannon, P.F. (1991) A Revision of Phyllachora and Some Similar Genera on the Host Family Leguminosae. CABI, Wallingford, UK, 302 pp.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Clements, F.E. & Shear, C.L. (1931) The Genera of Fungi, 2nd ed. Wilson Co, New York, NY, USA.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Dai, F.L. (1979) Sylloge Fungorum Sinicorum. Science Press, Beijing, China, 268 pp.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Dayarathne, M.C., Maharachchikumbura, S.S.N., Jones, E.B.G., Goonasekara, I.D., Bulgakov, T.S., Al-Sadi, A.M., Hyde, K.D., Lumyong, S. & McKenzie, E.H.C. (2017) Neophyllachora gen. nov. (Phyllachorales), three new species of Phyllachora from Poaceae and resurrection of Polystigmataceae (Xylariales). Mycosphere 8: 1598–1625. https://doi.org/10.5943/mycosphere/8/10/2</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Fuckel, L. (1870) Symbolae mycologicae. Beiträge zur kenntniss Der Rheinischen Pilze. Wiesbaden & Niedner, J. German, 459 pp. https://doi.org/10.5962/bhl.title.47117</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Hall, T.A. (1999) BioEdit: a user–friendly biological sequence alignment program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98. https://doi.org/10.1021/bk-1999-0734.ch008</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Katoh, K., Rozewicki, J. & Yamada, K.D. (2019) MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Bioinformatics 20: 1160–1166. https://doi.org/10.1093/bib/bbx108</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Kirk, P.M., Cannon, P.F., David, J.C. & Stalper, J.A. (2001) Ainsworth & Bisby’s Dictionary of the Fungi, 9th ed. CABI, Oxford, UK, 655 pp.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Kirk, P.M., Cannon, P.F., Minter, D.W. & Stalpers, J.A. (2008) Dictionary of Fungi, 10th ed. CABI, London, UK, 527 pp.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Lan, J.Q., Lu, H.J., Wu, J., Li, X.L., Zhang, T., Kong, C.C. & Kang, Z.S. (2012) Three new records of Phyllachora in China. Mycosystema 31: 639–641.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Li, J.C., Wu, H.X., Li, Y., Li, X.H., Song, J.Y., Suwannarach, N. & Wijayawardene, N.N. (2022) Taxonomy, phylogenetic and ancestral area reconstruction in Phyllachora, with four novel species from Northwestern China. Journal of Fungi 8: 520. https://doi.org/10.3390/jof8050520</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Li, X.L., Wu, S.R., Wang, C.L., Feng, Y.L., Zhao, C.Y., Chen, Z.Q., Yu, J.F., Luo, R., Promputtha, I. & Sun, D.F. (2019) Two new species of Phyllachora (Phyllachoraceae, Phyllachorales) on bamboo from China. Phytotaxa 425: 78–86. https://doi.org/10.11646/phytotaxa.425.2.2</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Li, X.L., Yang, Z.X., Wang, X.X., Yi, W. & Li, C.Y. (2018) Two new species of graminicolous Phyllachora (Phyllachoraceae, Ascomycota). Mycosystema 37: 1127–1132. https://doi.org/10.13346/j.mycosystema.180054</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Liu, N. & Li, M. (2016) Phyllachora jianfengensis sp. nov. from China. Mycotaxon 130: 1039–1043. https://dx.doi.org/10.5248/130.1039</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Liu, N., Wang, L., Gu, H. & Lin, G. (2015) Phyllachora hainanensis sp. nov. from China. Mycotaxon 130: 237–239. https://doi.org/10.5248/130.237</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Maharachchikumbura, S.S.N., Hyde, K.D., Jones, E.B.G., McKenzie, E.H.C. & Wijayawardene, N.N. (2016) Families of Sordariomycetes. Fungal Diversity 79: 1–317. https://dx.doi.org/10.1007/s13225-016-0369-6</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Mardones, M., Trampe-Jaschik, T., Oster, S., Elliott, M. & Piepenbring, M. (2017) Phylogeny of the order Phyllachorales (Ascomycota, Sordariomycetes): among and within order relationships based on five molecular loci. Persoonia 39: 74–90. https://dx.doi.org/10.3767/persoonia.2017.39.04</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: Miller, M.A., Pfeiffer, W. & Schwartz, T. (Eds.) 2010 Gateway Computing Environments Workshop. IEEE, New Orleans, Louisiana, USA, pp. 1–8.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Parbery, D.G. (1967) Studies on graminicolous species of Phyllachora Nke. in Fckl. V. A taxonomic monograph. Australian Journal of Botany 15: 271–375. https://dx.doi.org/10.1071/BT9670271</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Parbery, D.G. (1971) Studies on graminicolous species of Phyllachora Nke. in Fckl. VI. Additions and corrections to Part V. Australian Journal of Botany 19: 207–235. https://dx.doi.org/10.1071/BT9710207</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Parbery, D.G. & Langdon, R.N. (1964) Studies on graminicolous species of Phyllachora Fckl. IV. Evaluation of the critera of species. Australian Journal of Botany 12: 265–281. https://doi.org/10.1071/BT9640265</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Pearce, C.A., Reddell, P. & Hyde, K.D. (1999) A revision of Phyllachora (Ascomycotina) on hosts in the angiosperm family Asclepiadaceae, including P. gloriana sp. nov. on Tylophora benthamii from Australia. Fungal Diversity 3: 123–138. https://dx.doi.org/10.1071/SB00006</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Piepenbring, M., Hofmann, T.A., Kirschner, R., Mangelsdorff, R. & Trampe, T. (2011) Diversity patterns of Neotropical plant parasitic microfungi. Ecotropica 17: 27–40. [https://www.soctropecol.eu/PDF/Ecotropica_2011/Piepenbring_et_al_2011.pdf]</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Santos, M.D.M.D., Fonseca-Boiteux, M.E., Boiteux, L.S., Câmara, P.E.A.S. & Dianese, J.C. (2016) ITS–phylogeny and taxonomy of Phyllachora species on native Myrtacae from the Brazilian Cerrado. Mycologia 108: 1141–1164. https://dx.doi.org/10.3852/16-025</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Silva-Wanderlei, D., Neto, E.R. & Hanlin, R. (2003) Molecular systematics of the Phyllachorales (Ascomycota fungi) based on 18S ribosomal DNA sequences. Brasilian Archives of Biology and Technology 46: 315–322. https://doi.org/10.1590/S1516-89132003000300002</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Tamakaew, N., Maharachchikumbura, S., Hyde, K.D. & Cheewangkoon, R. (2017) Tar spot fungi from Thailand. Mycosphere 8: 1054–1058. https://dx.doi.org/10.5943/mycosphere/8/8/6</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Telenko, D.E.P., Ross, T.J., Shim, S., Wang, Q. & Singh, R. (2020) Draft genome sequence resource for Phyllachora maydis–an obligate pathogen that causes tar spot of corn with recent economic impacts in the United States. Molecular Plant–Microbe Interactions 33: 884–887. https://dx.doi.org/10.1094/MPMI-03-20-0075-A</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Teng, X.Q. (2020) Study on species diversity of Phyllachora in China. Agricultural Science-Technology and Information 3: 27–29. [In Chinese]</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238–4246. https://dx.doi.org/10.1128/jb.172.8.4238-4246.1990</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">White, T.J., Bruns, S., Lee, S. & Taylor, J. (1990) In: Michael, A. & David, H. (Eds.) PCR Protocols. Academic Press, London, UK, pp. 315–322. https://dx.doi.org/10.1016/B978-0-12-372180-8.50042-1</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Wu, H.X., Li, Y.M., Ariyawansa, H.A., Li, W.J. & Hyde, K.D. (2014) A new species of Microthyrium from Yunnan, China. Phytotaxa 176: 213–218. https://dx.doi.org/10.11646/phytotaxa.176.1.21</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Yang, C.L., Xu, X.L., Liu, Y.G., Hyde, K.D. & McKenzie, E.H.C. (2019) A new species of Phyllachora (Phyllachoraceae, Phyllachorales) on Phyllostachys heteroclada from Sichuan, China. Phytotaxa 3: 392. https://dx.doi.org/10.11646/phytotaxa.392.3.2</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Zhang, Z.Y. & Zhang, T. (2014) Flora Fungorum Sinicorum, Vol. 46, Phyllachora. Science Press, Beijing, China, 530 pp. [In Chinese]</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Cannon, P.F. (1991) A Revision of Phyllachora and Some Similar Genera on the Host Family Leguminosae. CABI, Wallingford, UK, 302 pp.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Clements, F.E. & Shear, C.L. (1931) The Genera of Fungi, 2nd ed. Wilson Co, New York, NY, USA.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Dai, F.L. (1979) Sylloge Fungorum Sinicorum. Science Press, Beijing, China, 268 pp.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Dayarathne, M.C., Maharachchikumbura, S.S.N., Jones, E.B.G., Goonasekara, I.D., Bulgakov, T.S., Al-Sadi, A.M., Hyde, K.D., Lumyong, S. & McKenzie, E.H.C. (2017) Neophyllachora gen. nov. (Phyllachorales), three new species of Phyllachora from Poaceae and resurrection of Polystigmataceae (Xylariales). Mycosphere 8: 1598–1625. https://doi.org/10.5943/mycosphere/8/10/2</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Fuckel, L. (1870) Symbolae mycologicae. Beiträge zur kenntniss Der Rheinischen Pilze. Wiesbaden & Niedner, J. German, 459 pp. https://doi.org/10.5962/bhl.title.47117</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Hall, T.A. (1999) BioEdit: a user–friendly biological sequence alignment program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98. https://doi.org/10.1021/bk-1999-0734.ch008</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Katoh, K., Rozewicki, J. & Yamada, K.D. (2019) MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Bioinformatics 20: 1160–1166. https://doi.org/10.1093/bib/bbx108</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Kirk, P.M., Cannon, P.F., David, J.C. & Stalper, J.A. (2001) Ainsworth & Bisby’s Dictionary of the Fungi, 9th ed. CABI, Oxford, UK, 655 pp.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Kirk, P.M., Cannon, P.F., Minter, D.W. & Stalpers, J.A. (2008) Dictionary of Fungi, 10th ed. CABI, London, UK, 527 pp.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Lan, J.Q., Lu, H.J., Wu, J., Li, X.L., Zhang, T., Kong, C.C. & Kang, Z.S. (2012) Three new records of Phyllachora in China. Mycosystema 31: 639–641.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Li, J.C., Wu, H.X., Li, Y., Li, X.H., Song, J.Y., Suwannarach, N. & Wijayawardene, N.N. (2022) Taxonomy, phylogenetic and ancestral area reconstruction in Phyllachora, with four novel species from Northwestern China. Journal of Fungi 8: 520. https://doi.org/10.3390/jof8050520</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Li, X.L., Wu, S.R., Wang, C.L., Feng, Y.L., Zhao, C.Y., Chen, Z.Q., Yu, J.F., Luo, R., Promputtha, I. & Sun, D.F. (2019) Two new species of Phyllachora (Phyllachoraceae, Phyllachorales) on bamboo from China. Phytotaxa 425: 78–86. https://doi.org/10.11646/phytotaxa.425.2.2</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Li, X.L., Yang, Z.X., Wang, X.X., Yi, W. & Li, C.Y. (2018) Two new species of graminicolous Phyllachora (Phyllachoraceae, Ascomycota). Mycosystema 37: 1127–1132. https://doi.org/10.13346/j.mycosystema.180054</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Liu, N. & Li, M. (2016) Phyllachora jianfengensis sp. nov. from China. Mycotaxon 130: 1039–1043. https://dx.doi.org/10.5248/130.1039</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Liu, N., Wang, L., Gu, H. & Lin, G. (2015) Phyllachora hainanensis sp. nov. from China. Mycotaxon 130: 237–239. https://doi.org/10.5248/130.237</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Maharachchikumbura, S.S.N., Hyde, K.D., Jones, E.B.G., McKenzie, E.H.C. & Wijayawardene, N.N. (2016) Families of Sordariomycetes. Fungal Diversity 79: 1–317. https://dx.doi.org/10.1007/s13225-016-0369-6</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Mardones, M., Trampe-Jaschik, T., Oster, S., Elliott, M. & Piepenbring, M. (2017) Phylogeny of the order Phyllachorales (Ascomycota, Sordariomycetes): among and within order relationships based on five molecular loci. Persoonia 39: 74–90. https://dx.doi.org/10.3767/persoonia.2017.39.04</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: Miller, M.A., Pfeiffer, W. & Schwartz, T. (Eds.) 2010 Gateway Computing Environments Workshop. IEEE, New Orleans, Louisiana, USA, pp. 1–8.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Parbery, D.G. (1967) Studies on graminicolous species of Phyllachora Nke. in Fckl. V. A taxonomic monograph. Australian Journal of Botany 15: 271–375. https://dx.doi.org/10.1071/BT9670271</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Parbery, D.G. (1971) Studies on graminicolous species of Phyllachora Nke. in Fckl. VI. Additions and corrections to Part V. Australian Journal of Botany 19: 207–235. https://dx.doi.org/10.1071/BT9710207</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Parbery, D.G. & Langdon, R.N. (1964) Studies on graminicolous species of Phyllachora Fckl. IV. Evaluation of the critera of species. Australian Journal of Botany 12: 265–281. https://doi.org/10.1071/BT9640265</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Pearce, C.A., Reddell, P. & Hyde, K.D. (1999) A revision of Phyllachora (Ascomycotina) on hosts in the angiosperm family Asclepiadaceae, including P. gloriana sp. nov. on Tylophora benthamii from Australia. Fungal Diversity 3: 123–138. https://dx.doi.org/10.1071/SB00006</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Piepenbring, M., Hofmann, T.A., Kirschner, R., Mangelsdorff, R. & Trampe, T. (2011) Diversity patterns of Neotropical plant parasitic microfungi. Ecotropica 17: 27–40. [https://www.soctropecol.eu/PDF/Ecotropica_2011/Piepenbring_et_al_2011.pdf]</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Santos, M.D.M.D., Fonseca-Boiteux, M.E., Boiteux, L.S., Câmara, P.E.A.S. & Dianese, J.C. (2016) ITS–phylogeny and taxonomy of Phyllachora species on native Myrtacae from the Brazilian Cerrado. Mycologia 108: 1141–1164. https://dx.doi.org/10.3852/16-025</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Silva-Wanderlei, D., Neto, E.R. & Hanlin, R. (2003) Molecular systematics of the Phyllachorales (Ascomycota fungi) based on 18S ribosomal DNA sequences. Brasilian Archives of Biology and Technology 46: 315–322. https://doi.org/10.1590/S1516-89132003000300002</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Tamakaew, N., Maharachchikumbura, S., Hyde, K.D. & Cheewangkoon, R. (2017) Tar spot fungi from Thailand. Mycosphere 8: 1054–1058. https://dx.doi.org/10.5943/mycosphere/8/8/6</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Telenko, D.E.P., Ross, T.J., Shim, S., Wang, Q. & Singh, R. (2020) Draft genome sequence resource for Phyllachora maydis–an obligate pathogen that causes tar spot of corn with recent economic impacts in the United States. Molecular Plant–Microbe Interactions 33: 884–887. https://dx.doi.org/10.1094/MPMI-03-20-0075-A</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Teng, X.Q. (2020) Study on species diversity of Phyllachora in China. Agricultural Science-Technology and Information 3: 27–29. [In Chinese]</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238–4246. https://dx.doi.org/10.1128/jb.172.8.4238-4246.1990</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">White, T.J., Bruns, S., Lee, S. & Taylor, J. (1990) In: Michael, A. & David, H. (Eds.) PCR Protocols. Academic Press, London, UK, pp. 315–322. https://dx.doi.org/10.1016/B978-0-12-372180-8.50042-1</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Wu, H.X., Li, Y.M., Ariyawansa, H.A., Li, W.J. & Hyde, K.D. (2014) A new species of Microthyrium from Yunnan, China. Phytotaxa 176: 213–218. https://dx.doi.org/10.11646/phytotaxa.176.1.21</span></span></span>
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