A new species and new records of Phlegmacium (Cortinariaceae) from China based on molecular and morphological evidence

FEI-FEI LIU; GANG WU; ZHU L. YANG

doi:10.11646/phytotaxa.578.2.1

Issue: Vol. 578 No. 2: 6 January 2023

Type: Article

Published: 2023-01-06

DOI: 10.11646/phytotaxa.578.2.1

Page range: 141-157

Abstract views: 485

PDF downloaded: 6

**A new species and new records of Phlegmacium (Cortinariaceae) from China based on molecular and morphological evidence**

FEI-FEI LIU⁺⁻
GANG WU⁺⁻
ZHU L. YANG⁺⁻

Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. Yunnan Key Laboratory for Fungal Diversity and Green Development, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China

Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. Yunnan Key Laboratory for Fungal Diversity and Green Development, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.

Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. unnan Key Laboratory for Fungal Diversity and Green Development, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.

Agaricales Cortinariaceae ITS Phlegmacium phylogeny taxonomy Fungi

Requires Subscription or Fee PDF (7MB)

Abstract

A new species, Phlegmacium ochraceosquamosum, is described from southwestern China based on morphological and molecular evidence. The new taxon is associated with subtropical fagaceous trees and is characterized by a combination of viscid pale yellow to ochraceous yellow pileus covered with ochraceous brown squamules and fibrils, cylindrical to clavate stipe with ochraceous brown fibrillose girdles, and elongate to subamygdaliform basidiospores. Four species, namely P. glaucopus, P. paracephalixum, P. patrickense, and P. violaceorubens, were first discovered in China. Additional vouchers for two species, namely P. caesiocolor and P. kytoevuorii very recently reported from China, were provided. Illustrated morphological descriptions and comparisons with closely related species are provided. The recognition of these species was further confirmed by phylogenetic analysis based on ITS sequences.

References

Ballarà, J., Mahiques, R. & Garrido-Benavent, I. (2017) Estudi de Cortinariaceae del Parc natural Cadí-Moixeró (IV). Moixeró 9: 20–49.

Bas, C. (1969) Morphology and subdivision of Amanita and a monograph of its section Lepidella. Persoonia 5 (4): 285–579.

Bidaud, A., Moënne-Loccoz, P. & Reumaux, P. (1994) Atlas des Cortinaires, Clé générale des sous-genres, sections, sous-sections et séries. Èditions Fèdération mycologique Dauphiné-Savoie, France.

Bidaud, A., Moënne-Loccoz, P., Reumaux, P., Carteret, X. & Eyssartier, G. (2006) Atlas des Cortinaires. Pars XVI. Èditions Fèdération mycologique Dauphiné-Savoie, France. pp. 1059–1122.

Bidaud, A. & Bellanger, J.M. (2016) À propos de Cortinarius daulnoyae Quél. The Journal of the J.E.C. 18: 13–23.

Bohus, G. (1976) Interessantere Cortinarius Arten aus dem Karpaten Becken. II. Ann Hist Nat Mus Natl Hung Termeszettud Muz Evk 68: 51–58.

Brandrud, T.E. (1996) Cortinarius subgenus Phlegmacium section Phlegmacium in Europe: descriptive part. Edinburgh Journal of Botany 53 (3): 331–400. https://doi.org/10.1017/S0960428600003772

Brandrud, T.E. (1998) Cortinarius subgenus Phlegmacium section Phlegmacioides (= Variicolores) in Europe. Edinburgh Journal of Botany 55 (1): 65–156. https://doi.org/10.1017/S0960428600004364

Brandrud, T.E., Lindström, H., Marklund, H., Melot, J. & Muskos, S. (1989) Cortinarius flora photographica. Vol. I (Swedish version). Cortinarius HB, Sweden.

Brandrud, T.E., Lindström, H., Marklund, H., Melot, J. & Muskos, S. (1992) Cortinarius flora photographica. Vol. II (Swedish version). Cortinarius HB, Sweden.

Brandrud, T.E., Lindström, H., Marklund, H., Melot, J. & Muskos, S. (1994) Cortinarius flora photographica. Vol. III (Swedish version). Cortinarius HB, Sweden.

Brandrud, T.E., Lindström, H., Marklund, H., Melot, J. & Muskos, S. (1998) Cortinarius flora photographica. Vol. IV (Swedish version). Cortinarius HB, Sweden.

Campo, E. (2015) Cortinarius paracephalixus e Cortinarius xanthosuavis dall’Italia centro-settentrionale. The Journal of the J.E.C. 17: 3–11.

Cheng, G.H., An, X.Y., Xie, M.L. & Li, Y. (2019) New records of Cortinarius species collected from Heilongjiang Province in China. Journal of Fungal Research 17 (2): 67–73. https://doi.org/10.13341/j.jfr.2019.1246

Cripps, C.L., Liimatainen, K., Niskanen, T., Dima, B., Bishop, R.F. & Ammirati, J.F. (2015) Intercontinental distributions of species of Cortinarius, subgenus Phlegmacium, associated with Populus in western North America. Botany 93 (11): 711–721. https://doi.org/10.1139/cjb-2015-0089

Dai, Y.C., Zhou, L.W., Yang, Z.L., Wen, H.A., Bau, T. & Li, T.H. (2010) A revised checklist of edible fungi in China. Mycosystema 29 (1): 1–21. https://doi.org/10.13346/j.mycosystema.2010.01.022

Earle, F.S. (1909) The genera of the North American gill fungi. Bulletin of the New York Botanical Garden 5: 373–451.

Fayod, V. (1889) Podrome d’une histoire naturelle des Agaricinés. Annales des Sciences Naturelles; Botanique VII 9: 181–411.

Fernandez-Brime, S., Vila, J. & Ortega, A. (2014) Some new and interesting taxa of Cortinarius subgenus Phlegmacium from the European Mediterranean Basin. Mycologia 106 (3): 491–504. https://doi.org/10.3852/13-177

Fries, E.M. (1821) Systema mycologicum. Uppsala, Sweden. 520 pp.

Fries, E.M. (1836–1838) Epicrisis systematis mycologici seu synopsis Hymenomycetum. Uppsala, Sweden.

Fries, E.M. (1878–1884) Icones selectae Hymenomycetum nondum delineatorum. II. Holmiae et Upsaliae, Sweden.

Gardes, M. & Bruns, T.D. (1993) ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts. Molecular Ecology 2 (2): 113–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x

Garnica, S., Weiß, M., Oertel, B. & Oberwinkler, F. (2003) Phylogenetic relationships of european Phlegmacium species (Cortinarius, Agaricales). Mycologia 95 (6): 1155–1170. https://doi.org/10.2307/3761917

Garnica, S., Weiß, M., Oertel, B. & Oberwinkler, F. (2005) A framework for a phylogenetic classification in the genus Cortinarius (Basidiomycota, Agaricales) derived from morphological and molecular data. Canadian Journal of Botany 83 (11): 1457–1477. https://doi.org/10.1139/b05-107

Gasparini, B. (2007) Genus Cortinarius, subgenus Phlegmacium in Tasmania. New Zealand Journal of Botany 45 (1): 155–236.

Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.

Harrower, E., Ammirati, J.F., Cappuccino, A.A., Ceska, O., Kranabetter, J.M., Kroeger, P., Lim, S.R., Taylor, T. & Berbee, M.L. (2011) Cortinarius species diversity in British Columbia and molecular phylogenetic comparison with European specimen sequences. Botany 89 (11): 799–810. https://doi.org/10.1139/b11-065

Henry, R. (1986) Suite a l’étude des Cortinaires. Bulletin de la Société Mycologique de France 102 (1): 19–96.

Horak, E. (1983) Mycogeography in the South Pacific region: Agaricales, Boletales. Australian Journal of Botany, Supplementary Series 13 (10): 1–42.

Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology Evolution 30 (4): 772–780. https://doi.org/10.1093/molbev/mst010

Khan, J., Sher, H., Naseer, A. & Khalid, A.N. (2017) Descolea quercina (Bolibitaceae), a new species from moist temperate forests in Pakistan. MycoKeys 27: 65–76. https://doi.org/10.3897/mycokeys.27.14730

Kornerup, A. & Wanscher, J.H. (1981) Taschenlexikon der Farben. 3rd edn. Muster-Schmidt Verlag, Zürich und Göttingen.

Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33 (7): 1870–1874. https://doi.org/10.1093/molbev/msw054

Kytövuori, I., Niskanen, T., Liimatainen, K. & Lindström, H. (2005) Cortinarius sordidemaculatus and two new related species, C. anisatus and C. neofurvolaesus, in Fennoscandia (Basidiomycota, Agaricales). Karstenia 45 (1): 33–49. https://doi.org/10.29203/ka.2005.402

Liimatainen, K., Niskanen, T., Dima, B., Kytövuori, I., Ammirati, J.F. & Frøslev, T.G. (2014) The largest type study of Agaricales species to date: bringing identification and nomenclature of Phlegmacium (Cortinarius) into the DNA era. Persoonia 33 (1): 98–140. https://doi.org/10.3767/003158514X684681

Liimatainen, K., Kim, J.T., Pokorny, L., Kirk, P.M., Dentinger, B. & Niskanen, T. (2022) Taming the beast: a revised classification of Cortinariaceae based on genomic data. Fungal Diversity 112 (1): 89–170. https://doi.org/10.1007/s13225-022-00499-9

Luo, Y. & Bau, T. (2021) Cortinarius jiaoheensis (Cortinariaceae), a new species of Cortinarius subgenus Telamonia section Flexipedes, from northeast China. Phytotaxa 494 (1): 113–121. https://doi.org/10.11646/phytotaxa.494.1.7

Mao, X.L. (2000) The macrofungi of China. Henan Science & Technology Press, Zhengzhou. 719 pp.

Matheny, P.B., Curtis, J.M., Hofstetter, V., Aime, M.C., Moncalvo, J.M., Ge, Z.W., Yang, Z.L., Slot, J.C., Ammirati, J.F., Baroni, T.J., Bougher, N.L., Hughes, K.W., Lodge, D.J., Kerrigan, R.W., Seidl, M.T., Aanen, D.K., DeNitis, M., Daniele, G.M., Desjardin, D.E., Kropp, B.R., Norvell, L.L., Parker, A., Vellinga, E.C., Vilgalys, R. & Hibbett, D.S. (2006) Major clades of Agaricales: a multilocus phylogenetic overview. Mycologia 98 (6): 982–995. https://doi.org/10.1080/15572536.2006.11832627

Moënne-Loccoz, P., Reumaux, P. & Bidaud, A. (1990–2001) Atlas des Cortinaires 1–11. Editions Fédération mycologique Daupiné-Savoie, France.

Moser, M.M. (1960) Die Gattung Phlegmacium (Schleimköpfe). Die Pilze Mitteleuropa. Band IV. Bad Heilbrunn, Germany. 440 pp. 

Moser, M.M. (1983) Die Röhrlinge und Bläitterpilze. In: Gams, H. (Ed.) Kleine Kryptogamenflora. Band II b/2. 5th ed. Gustav Fischer Verlag, Stuttgart. 532 pp.

Noordeloos, M.E., Vellinga, E.C. & Kuyper, TH.W. (2018) Chapter I. In: Noordeloos, M.E., Kuyper, TH.W., Somhorst, I. & Vellinga, E.C. (Eds.) Flora Agaricina Neerlandica. Vol. 7. Candusso Editrice, Italy.

Nylander, J.A.A. (2004) MrModeltest v2. Program distributed by the author. Evolutionary Biology Center, Uppsala University. 

Ortega, A., Suárez-Santiago, V.N. & Vila, J. (2009) Two new species of Cortinarius collected under Quercus rotundifolia in the Mediterranean area of southern Spain. Fungal Diversity 36: 89.

Orton, P.D. (1955) The genus Cortinarius I. Myxacium and Phlegmacium. Naturalist (July – Sept): 1–80. 

Ricken, A. (1915) Die Blätterpilze (Agaricaceae) Deutschlands und der angrenzenden Länder, besonders Oesterreichs und der Schweiz. Vol. II. Theodor Oswald Weigel, Leipzig. 480 pp.

Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61 (3): 539–542. https://doi.org/10.1093/sysbio/sys029

Singer, R. & Digilio, A.P. (1951) Pródromo de la flora agaricina Argentina. Lilloa 25: 5–461.

Soop, K. (2004) Cortinarius in Sweden. 9th rev. ed. Verlag, Scientrix, Sweden. 83 pp. 

Soop, K. (2005) Cortinarius in Sweden. 10th rev. ed. Verlag, Scientrix, Sweden. 100 pp.

Soop, K., Schmidt-Stohn, G., Dima, B., Saar, G. & Brandrud, T.E. (2019) Cortinarius subgenus Phlegmacium section Aureocistophili in Europe. The Journal of the J.E.C. 21: 33–54.

Stamatakis, A. (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30: 1312–1313.

Vila, J., Ortega, A., Suárez-Santiago, V.N. & Llimona, X. (2008) Cortinarius mahiquesii, a new subhypogeous species from Catalonia (Iberian Peninsula). Persoonia 21 (1): 153–157. https://doi.org/10.3767/003158508X388380

Wang, K., Xie, M.L., Liu, T.Z., Li, G.J., Liu, D.M. & Wei, T.Z. (2022) Taxonomy of Cortinarius in China 1. Subgenus Phlegmacium. Mycosystema 41 (9): 1370–1397. https://doi.org/10.13346/j.mycosystema.210494

Wei, T.Z. & Yao, Y.J. (2013) Cortinarius korfii, a new species from China. Mycosystema 32 (3): 557–562. https://doi.org/10.13346/j.mycosystema.2013.03.017

White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR protocols: a guide to methods and applications. Academic Press, New York. pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1

Xie, M.L., Li, D., Wei, S.L., Ji, R.Q. & Li, Y. (2019) Cortinarius subcaesiobrunneus sp. nov., (Cortinariaceae, Agaricales) a new species from northwest China. Phytotaxa 392 (3): 217–224. https://doi.org/10.11646/phytotaxa.392.3.4

Xie, M.L., Wei, T.Z., Fu, Y.P., Li, D., Qi, L.L., Xing, P.J., Cheng, G.H., Ji, R.Q. & Li, Y. (2020) Three new species of Cortinarius subgenus Telamonia (Cortinariaceae, Agaricales) from China. MycoKeys 69: 91–109. https://doi.org/10.3897/mycokeys.69.49437

Xie, M.L., Chen, J.L., Phukhamsakda, C., Dima, B., Fu, Y.P., Ji, R.Q., Wang, K., Wei, T.Z. & Li, Y. (2021a) Cortinarius subsalor and C. tibeticisalor spp. nov., two new species from the section Delibuti from China. PeerJ 9: e11982. https://doi.org/10.7717/peerj.11982

Xie, M.L., Wei, T.Z., Dima, B., Fu, Y.P., Ji, R.Q. & Li, Y. (2021b) Cortinarius khinganensis (Agaricales), a new species of section Illumini from Northeast China. Phytotaxa 500 (1): 1–10. https://doi.org/10.11646/phytotaxa.500.1.1

Xie, M.L., Phukhamsakda, C., Wei, T.Z., Li, J.P., Wang, K., Wang, Y., Ji, R.Q. & Li, Y. (2022) Morphological and phylogenetic evidence reveal five new Telamonioid species of Cortinarius (Agaricales) from East Asia. Journal of Fungi 8 (3): 257. https://doi.org/10.3390/jof8030257

Yang, Z.L. (1998) Revision of the genera Rozites and Descolea from China. Fungal Science 13 (3 & 4): 61–74.

Yuan, H.S., Lu, X., Dai, Y.C., Hyde, K.D., Kan, Y.H., Kušan, I., He, S.H., Liu, N.G., Sarma, V.V., Zhao, C.L., Cui, B.K., Yousaf, N., Sun, G., Liu, S.Y., Wu, F., Lin, C.G., Dayarathne, M.C., Gibertoni, T.B., Conceição, L.B., Garibay-Orijel, R., Villegas-Ríos, M., Salas-Lizana, R., Wei, T.Z., Qiu, J.Z., Yu, Z.F., Phookamsak, R., Zeng, M., Paloi, S., Bao, D.F., Abeywickrama, P.D., Wei, D.P., Yang, J., Manawasinghe, I.S., Harishchandra, D., Brahmanage, R.S., de Silva, N.I., Tennakoon, D.S., Karunarathna, A., Gafforov, Y., Pem, D., Zhang, S.N., de Azevedo, Santiago, A.L.C.M., Bezerra, J.D.P., Dima, B., Acharya, K., Alvarez-Manjarrez, J., Bahkali, A.H., Bhatt, V.K., Brandrud, T.E., Bulgakov, T.S., Camporesi, E., Cao, T., Chen, Y.X., Chen, Y.Y., Devadatha, B., Elgorban, A.M., Fan, L.F., Du, X., Gao, L., Gonçalves, C.M., Gusmão, L.F.P., Huanraluek, N., Jadan, M., Jayawardena, R.S., Khalid, A.N., Langer, E., Lima, D.X., de Lima-Júnior, N.C., de Lira, C.R.S., Liu, J.K., Liu, S., Lumyong, S., Luo, Z.L., Mato?ec, N., Niranjan, M., Oliveira-Filho, J.R.C., Papp, V., Pérez-Pazos, E., Phillips, A.J.L., Qiu, P.L., Ren, Y., Ruiz, R.F.C., Semwal, K.C., Soop, K., de Souza, C.A.F., Souza-Motta, C.M., Sun, L.H., Xie, M.L., Yao, Y.J., Zhao, Q. & Zhou, L.W. (2020) Fungal diversity notes 1277–1386: taxonomic and phylogenetic contributions to fungal taxa. Fungal Diversity 104 (1): 1–266. https://doi.org/10.1007/s13225-020-00461-7

How to Cite

Liu, F.-F., Wu, G. & Yang, Z.L. (2023)

A new species and new records of Phlegmacium (Cortinariaceae) from China based on molecular and morphological evidence

. Phytotaxa 578 (2): 141–157. https://doi.org/10.11646/phytotaxa.578.2.1

A new species and new records of Phlegmacium (Cortinariaceae) from China based on molecular and morphological evidence

Abstract

References

How to Cite

Download Citation

**A new species and new records of Phlegmacium (Cortinariaceae) from China based on molecular and morphological evidence**