Abstract
A novel mushroom species occurring on loamy soil under Bombax cieba was collected from Chichawatni forest, Punjab, Pakistan. Phylogenetic analyses of the nrDNA ITS and nuLSU regions revealed that it clusters within the genus Clitopilus. Further morpho-anatomical analyses confirmed its uniqueness, which is referred as a new species under the name Clitopilus chichawatniensis. The distinguishing characteristics of this novel taxon are large whitish to light pale, eccentric pleurotoid pileus 50–65 mm wide with lobed and involuted margins, large ovoid to ellipsoid basidiospores 6.2–8.4 × 4.8–6.4 µm with 3–5 longitudinal ridges, absence of cheilocystidia and pleurocystidia. A detailed description of this taxon and a key to all existing species of the genus Clitopilus in Pakistan is provided.
References
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<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Baroni, T.J., Hofstetter, V., Largent, D.L. & Vilgalys, R. (2011) Entocybe is proposed as a new genus in the Entolomataceae (Agaricomycetes, Basidiomycota) based on morphological and molecular evidence. North American Fungi 6 (12): 1–19. https://doi.org/10.2509/naf2011.006.012</span></span></span>
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<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Baroni, T.J. & Watling, R. (1999) Taxonomic and mycogeographic notes on some Malaysian fungi IV. Notes on Clitopilus and Rhodocybe. Mycotaxon 72: 57–72.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Baroni, T.J., Claudio, A., Bergemann, S.E., Lodge, D.J., Lacey, L., Curtis, T.A. & Cantrell, S.A. (2020) Rhodocybe-Clitopilus clade (Entolomataceae, Basidiomycota) in the Dominican RePublic: new taxa and first reports of Clitocella, Clitopilus, and Rhodocybe for Hispaniola. Mycological Progress 19: 1083–1099. https://doi.org/10.1007/s11557-020-01619-y</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Co-David, D., Langeveld, D. & Noordeloos, M.E. (2009) Molecular phylogeny and spore evolution of Entolomataceae. Persoonia 23: 147–176. https://doi.org/10.3767/003158509X480944</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Cooper, J.A. (2014) New species and combinations of some New Zealand agarics belonging to Clitopilus, Lyophyllum, Gerhardtia, Clitocybe, Hydnangium, Mycena, Rhodocollybia and Gerronema. Mycosphere 5 (2): 263–288. https://doi.org/10.5943/mycosphere/5/2/2</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Gardes, M. & Bruns, T.D. (1993) ITS primers with enhanced specificity for basidiomycetes, application to the identification of mycorrhizae and rusts. Molecular Ecology 2: 113–118.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Giusti, A., Ricci, E., Gasperetti, L., Galgani, M., Polidori, L., Verdigi, F., Narduci, R. & Armani, A. (2021) Building of an Internal Transcribed Spacer (ITS) Gene Dataset to Support the Italian Health Service in Mushroom Identification. Foods 10 (6): 1193. https://doi.org/10.3390/foods10061193</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Gouy, M., Guindon, S. & Gascuel, O. (2010) SeaView version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building. Molecular biology and evolution 27 (2): 221–224. https://doi.org/10.1093/molbev/msp259</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 45: 95–98.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Hartley, A.J., de Mattos-Shipley, K., Collins, C.M., Kilaru, S., Foster, G.D. & Bailey, A.M. (2009) Investigating pleuromutilin-producing Clitopilus species and related basidiomycetes. FEMS Microbiology Letters 297 (1): 24–30. https://doi.org/10.1111/j.1574-6968.2009.01656.x</span></span></span>
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<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Jian, S.P., Bau, T., Zhu<span lang="en-GB">,</span> X.T., Deng, W.Q., Yang, Z.L. & Zhao, Z.W. (2020a) Clitopilus, Clitocella, and Clitopilopsis in China. Mycologia 112 (2): 371–399. https://doi.org/10.1080/00275514.2019.1703089</span></span></span>
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