Abstract
Impatiens longyangensis and I. yaojiapingensis, two new species from Gaoligong Mountains, Yunnan, China, are described here. The former species is morphologically similar to I. shangjiangensis, but differs by its shorter petioles and peduncle, ovate bract, shortly saccate lower sepal with a longer spur and suborbicular dorsal petal. The latter is similar to I. sterilis, and differs by its pubescent leaf, petiole with one to three pairs of clavate glands, persistent bract, lower sepal with a straight spur and suborbicular dorsal petal. Molecular phylogenetic analysis based on nuclear ITS and plastid atpB-rbcL sequences shows that the two new species belong to I. sect. Racemosae.
References
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Chen, Y.L., Akiyama, S. & Ohba, H. (2007) Balsaminaceae. In: Wu, Z.Y. & Raven, P.H. (eds.) Flora of China, vol. 12. Science Press, Beijing & Missouri Botanical Garden Press, St?Louis, pp. 43–114</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Cong, Y.Y. (2007) Study on the flora of Impatiens L. in the Gaoligong Mountains region, Yunnan. Master’s Thesis, Hunan Normal University, Changsha, 52 pp.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Forst, J.R. & Forst, G. (1776) Characteres generum plantarum, quas in itinere ad insulas maris Australis, 150 pp. https://doi.org/10.5962/bhl.title.4448</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Gu, J.Z., Yang, N., Peng, Y.C., Song, Y.X., Guo, S.W. & Cong, Y.Y. (2021) Impatiens shangjiangensis (Balsaminaceae), a new species from Yunnan, China. Annales Botanici Fennici 58: 197–201. http://dx.doi.org/10.5735/085.058.0401 </span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Guindon, S., Dufayard, J.F., Lefort, V., Anisimova, M., Hordijk, W. & Gascuel, O. (2010) New Algorithms and Methods to Estimate Maximum-Likelihood Phylogenies: Assessing the Performance of PhyML 3.0. Systematic Biology 59 (3): 307–321. https://dx.doi.org/10.1093/sysbio/syq010</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Handel-Mazzetti. (1933) Symbolae Sinicae, vol. 7. Springer, J., Wien, 2364 pp. https://doi.org/10.5962/bhl.title.878</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">IUCN Standards and Petitions Subcommittee (2022) Guidelines for using the IUCN Red List Categories and Criteria. Version 15. Available from: https://nc.iucnredlist.org/redlist/content/attachment_files/RedListGuidelines.pdf (accessed January 2022)</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Janssens, S., Geuten, K., Yuan, Y.M., Song, Y., Küpfer, P. & Smets, E. (2006) Phylogenetics of Impatiens and Hydrocera (Balsaminaceae) using chloroplast atpB-rbcL spacer sequences. Systematic Botany 31 (1): 171–180. https://doi.org/10.1600/036364406775971796</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Kalyaanamoorthy, S., Minh, B.Q., Wong, T.K., von Haeseler, A. & Jermiin, L.S. (2017) ModelFinder: Fast model selection for accurate phylogenetic estimates. Nature Methods 14 (6): 587–589. https://doi.org/10.1038/nmeth.4285</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. (2018) MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Molecular Biology and Evolution 35 (6): 1547–1549. https://doi.org/10.1093/molbev/msy096</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Li, H., Huang, H.Q. & Huang, M.J. (2013) Investigation and application of Impatiens L. resource in Yunnan. Northern Horticulture 10: 91–94.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Liao, R.L., Cai, L., Yu, Z.Y., Wang, Y.H. & Sun, W.B. (2021) Impatiens wutaishanensis (Balsaminaceae), a new species from Southeast Yunnan, China. PhytoKeys 176: 43–53. https://doi.org/10.3897/phytokeys.176.58825</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Linnaeus, C. (1753) Species Plantarum. Salvius, Stockholm, 1200 pp. https://doi.org/10.5962/bhl.title.669</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Liu, C.J., Lin, Q. & He, J.X. (2004) Methods and terminology of study on seed morphology from China. Acta Botanica Boreali-occidentalia Sinica 24: 178–188.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Lu, Y.Q. (1991) Pollen morphology of Impatiens L. (Balsaminaceae) and its taxonomic implications. Journal of Systematics and Evolution 29 (4): 352–357. </span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Lu, Y.Q. & Chen, Y.L. (1991) Seed morphology of Impatiens L. (Balsaminaceae) and its taxonomic implications. Journal of Systematics and Evolution 29 (3): 252–257</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Myers, N., Mittermeier, R.A., Mittermeier, C.G., da Fonseca, G.A.B. & Kent, J. (2000) Biodiversity hotspots for conservation priorities. Nature 403: 853–858. https://doi.org/10.1038/35002501</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Nees, E. (1836) Systema laurinarum. Berolini, Sumptibus Veitii et Sociorum, 720 pp. https://doi.org/10.5962/bhl.title.422</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Nguyen, L.T., Schmidt, H.A., von Haeseler, A. & Minh, B.Q. (2014) IQ-TREE: A Fast and Effective Stochastic Algorithm for Estimating Maximum-Likelihood Phylogenies. Molecular Biology and Evolution 32 (1): 268–274. https://doi.org/10.1093/molbev/msu300</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Peng, S., Cong, Y.Y., Tian, J., Zhang, C.F., Hu, G.W. & Wang, Q.F. (2021a) Impatiens bullatisepala (Balsaminaceae), a new species from Guizhou, China. Phytotaxa 500 (3): 217–224. https://doi.org/10.11646/phytotaxa.500.3.5</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Peng, S., Rono, P.C., Yang, J.X., Wang, J.J., Hu, G.W. & Wang, Q.F. (2021b) Description of a new species and lectotypification of two names in Impatiens sect. Racemosae (Balsaminaceae) from China. Plants 10 (9): 1812. https://doi.org/10.3390/plants10091812</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Qian, L.S., Chen, J.H., Deng, T. & Sun, H. (2020) Plant diversity in Yunnan: Current status and future directions, Plant Diversity 42 (4): 281–291. https://doi.org/10.1016/j.pld.2020.07.006.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Ren, L.Y., Chen, Y., Yuan, T.H., Huang, R.X., Li, M.J. & Bai, X.X. (2022) Impatiens bijieensis (Balsaminaceae), a new species from karst plateau in Guizhou, China. PhytoKeys 192: 1–10. https://doi.org/10.3897/phytokeys.192.77517</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Ronquist, F., Teslenko, M., Der Mark, P.V., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: Efficient Bayesian Phylogenetic Inference and Model Choice Across a Large Model Space. Systematic Biology 61 (3): 539–542. https://doi.org/10.1093/sysbio/sys029</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Shui, Y.M., Janssens, S., Huang, S.H., Chen, W.H. & Yang, Z.G. (2011) Three new species of Impatiens. from China and Vietnam: preparation of flowers and morphology of pollen and seeds. Systematic Botany 36 (2): 428–439. https://doi.org/10.1600/036364411X569615</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Song, Y.X., Xiao, Y., Peng, S., Cong, Y.Y. & Hu, G.W. (2021a) Two new species of Impatiens from China, and taxonomic insights into the Longifilamenta Group, which is endemic to China. Plants 10 (8): 1697. https://doi.org/10.3390/plants10081697</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Song, Y.X., Cong, Y.Y., Kuang, R.P., Peng, Y.C. & Zheng, Y.M. (2021b) Impatiens sterilis, a rare new species of Impatiens with reduced sterile inflorescences from Yunnan, China. Taiwania 66 (1): 48–52. https://doi.org/10.6165/tai.2021.66.48</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Song, Y.X., Peng, S., Cong, Y.Y. & Zheng, Y.M. (2021c) Impatiens rapiformis, a new species of Impatiens with root tuber from Yunnan, China. Nordic Journal of Botany 39 (5): e03151. https://doi.org/10.1111/njb.03151</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Wang, J.S., Lu, Y.F., Xu, Y.L., Jin, S.H. & Jin, X.F. (2020) Impatiens wuyiensis (Balsaminaceae), a new species from Fujian of Southeast China, based on morphological and molecular evidences. Botanical Studies 61 (1): 1–12. https://doi.org/10.1186/s40529-020-00306-1</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Wang, K.F. & Wang, X.Z. (1983) Outline of palynology. Peking University Press, Beijing, 205 pp.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Wang, Z., Wu, P.P., Liu, C.C., Guo, K., Yu, S.X. (2022) Impatiens nushanensis (Balsaminaceae), a new species from Yunnan, China. Phytotaxa 545 (2): 186–196. https://doi.org/10.11646/phytotaxa.545.2.7</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Wu, Z.Y. (2006) Balsaminaceae. In: Wu, Z.Y. (ed.) Flora of Yunnan vol. 16. Science Press, Beijing, 892 pp.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Xia, C.Y., Gadagkar, S.R., Li, J., Deng, H.P. & Xiang, S. (2019) Impatiens damingensis (Balsaminaceae), a new species from Guangxi, China. Phytotaxa 399 (3): 239–247. https://doi.org/10.11646/phytotaxa.399.3.6</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Yu, S.X. (2012) Balsaminaceae of China. Peking University Press, Beijing, 215 pp.</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Yu, S.X., Janssens, S.B., Zhu, X.Y., Lidén, M., Gao, T.G. & Wang, W. (2016) Phylogeny of Impatiens (Balsaminaceae): integrating molecular and morphological evidence into a new classification. Cladistics 32: 179–197. https://doi.org/10.1111/cla.12119</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Yuan, T.H., Chen, Y., Yu, S., Ren, L.Y., Huang, R.X., Li, M.J. & Bai, X.X. (2022) Impatiens liupanshuiensis (Balsaminaceae), a new species from Guizhou, China. PhytoKeys 192: 37–44. https://doi.org/10.3897/phytokeys.192.77269</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Yuan, T., Li, M., Ren, L., Huang, R., Chen, Y. & Bai, X. (2022) A dataset on the diversity and geographical distributions of wild Impatiens in China. Biodiversity Science 30 (5): 22019. https://doi.org/10.17520/biods.2022019</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Yuan, Y.M., Song, Y., Geuten, K., Rahelivolona, E., Wohlhauser, S., Fischer, E., Smets, E. & Küpfer, P. (2004) Phylogeny and biogeography of Balsaminaceae inferred from ITS sequence data. Taxon 53: 391–403. https://doi.org/10.2307/4135617</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Zhang, C.F., Peng, S., Tian, J., Hu, G.W. & Wang, Q.F. (2020) A new species and a newly recored species of Impatiens (Balsaminaceae) from Yunnan, China. Plant Science Journal 38 (4): 437–447. https://doi.org/10.11913/PSJ.2095-0837.2020.40437</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Zhang, D., Gao, F.L., Jakovli, I., Zou, H., Zhang, J. & Wang, G.T. (2020) PhyloSuite: An integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies. Molecular Ecology Resources 20 (1): 348–355. https://doi.org/10.1101/489088</span></span></span>
<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Zou, C.Y., Liu, Y., Li, J. & Yu, S.X. (2020) Impatiens plicatisepala (Balsaminaceae), a new species from Guangxi, China. Taiwania 65 (4): 544?547. https://doi.org/10.6165/tai.2020.65.544</span></span></span>
