Cunninghamella verrucosa sp. nov. (Mucorales, Mucoromycota) from Guangdong Province in China

YU-JIE WANG; TING ZHAO; WEN-YING WU; MU WANG; XIAO-YONG LIU

doi:10.11646/phytotaxa.560.3.2

Issue: Vol. 560 No. 3: 2 September 2022

Type: Article

Published: 2022-09-02

DOI: 10.11646/phytotaxa.560.3.2

Page range: 274-284

Abstract views: 92

PDF downloaded: 2

Cunninghamella verrucosa sp. nov. (Mucorales, Mucoromycota) from Guangdong Province in China

YU-JIE WANG⁺⁻
TING ZHAO⁺⁻
WEN-YING WU⁺⁻
MU WANG⁺⁻
XIAO-YONG LIU⁺⁻

College of Plant Science, Tibet Agricultural and Animal Husbandry University, Linzhi 860000, China; College of Life Sciences, Shandong Normal University, Jinan 250358, China

China Alcoholic Drinks Association, Beijing 100831, China

College of Plant Science, Tibet Agricultural and Animal Husbandry University, Linzhi 860000, China

College of Life Sciences, Shandong Normal University, Jinan 250358, China; State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China

Cunninghamellaceae Early diverging fungi Fungal diversity Phylogeny Taxonomy Fungi

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Abstract

Species of Cunninghamella are ubiquitous in soil, flower and plant debris. Its metabolites have various biological activities, such as antifungal and antibacterial functions. In this study, we describe a new species C. verrucosa from soil in Guangdong Province, China. This species is characterized by one to several broken pedicels on the surface of clavate vesicles, unbranched or simply branched sporangiophores, and globose sporangiola. Phylogenetic analyses strongly show that C. verrucosa is sister to C. clavata. Together with this new species, a total of 22 taxa, including 19 species and three varieties, has been described in Cunninghamella. Accordingly, an updated key to the taxa of Cunninghamella is also provided in this study.

References

Alasmary, F.A., Awaad, A.S., Alqahtani, S.M., El-Meligy, R.M., Abdullah, D.A. & Alqasoumi, S.I. (2020) Evaluation of the chemical constituents and potential biological activities of Cunninghamella blakesleeana. Saudi Pharmaceutical Journal 28: 1197–1202. https://doi.org/10.1016/j.jsps.2020.08.009
Alves, A., de Souza, C., de Oliveira, R., Cordeiro, T. & de Santiago, A. (2017) Cunninghamella clavata from Brazil: a new record for the western hemisphere. Mycotaxon 132: 381–389. https://doi.org/10.5248/132.381
Cha, C.J., Doerge, D.R. & Cerniglia, C.E. (2001) Biotransformation of malachite green by the fungus Cunninghamella elegans. Applied and Environmental Microbiololgy 67: 4358–4360. https://doi.org/10.1128/aem.67.9.4358-4360.2001
Dai, Y.C. (2010) Hymenochaetaceae (Basidiomycota) in China. Fungal Diversity 45: 131–343. https://doi.org/10.1007/s13225-010-0066-9
Elkhateeb, W.A., Elnahas, M.O. & Daba, G.M. (2021) Bioactive metabolites of Cunninghamella, biodiversity to biotechnology. Journal of Pharmaceutics and Pharmacology Research 4: 1–5. https://doi.org/10.31579/2693-7247/036
GBIF-Secretariat (2021) GBIF Backbone Taxonomy. Checklist dataset. Via GBIF.org. Available at https://doi.org/10.15468/39omei. (Accessed on April 25, 2022)
Guo, J., Wang, H., Liu, D., Zhang, J.N., Zhao, Y.H., Liu, T.X. & Xin, Z.H. (2015) Isolation of Cunninghamella bigelovii sp. nov. CGMCC 8094 as a new endophytic oleaginous fungus from Salicornia bigelovii. Mycological Progress 14: 1–8. https://doi.org/10.1007/s11557-015-1029-z
Hallur, V., Prakash, H., Sable, M., Preetam, C., Purushotham, P., Senapati, R., Shankarnarayan, S.A., Bag, N.D. & Rudramurthy, S.M. (2021) Cunninghamella arunalokei a new species of Cunninghamella from India causing disease in an immunocompetent individual. Journal of Fungi 7: 670. https://doi.org/10.3390/jof7080670
Hyde, K.D., Hongsanan, S., Jeewon, R., Bhat, J.D., McKenzie, E.H.C., Jones, E.B.G., Phookamsak, R., Ariyawansa, H.A., Boonmee, S., Zhao, Q., Abdel-Aziz, F.A., Abdel-Wahab, M.A., Banmai, S., Chomnunti, P., Cui, B.K., Daranagama, D.A., Das, K., Dayarathne, M.C., De Silva, N.I., Dissanayake, A.J., Doilom, M., Ekanayaka, A.H., Gibertoni, T.B., Go´es-Neto, A., Huang, S.K., Jayasiri, S.C., Jayawardena, R., Konta, S., Lee, H.B., Li, W.J., Lin, C.G., Liu, J.K., Lu, Y.Z., Luo, Z.L., Manawasinghe, I.S., Manimohan, P., Mapook, A., Niskanen, T., Norphanphoun, C., Papizadeh, M., Perera, R.H., Phukhamsakda, C., Richter, C., de A. Santiago, A.L.C.M., Drechsler-Santos, E.R., Senanayake, I.C. & Tanaka, K. (2016) Fungal diversity notes 367–490: taxonomic and phylogenetic contributions to fungal taxa. Fungal Diversity 80: 1–270. https://doi.org/10.1007/s13225-016-0373-x
Kearse, M,, Moir, R., Wilson, A., Stones-Havas, S., Cheung, M., Sturrock, S., Buxton, S., Cooper, A., Markowitz, S. & Duran, C. (2012) Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28: 1647–1649. https://doi.org/10.1093/bioinformatics/bts199
Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30: 772–780. https://doi.org/10.1093/molbev/mst010
Liu, C.W., Liou, G.Y. & Chien, C.Y. (2005) New records of the genus Cunninghamella (Mucorales) in Taiwan. Fungal Science 20: 1–9
Liu, X.Y., Huang, H. & Zheng, R.Y. (2001) Relationships within Cunninghamella based on sequence analysis of ITS rDNA. Mycotaxon 80: 77–95
Nguyen, T.T.T, Choi, Y.J. & Lee, H.B. (2017) Isolation and characterization of three unrecorded Zygomycete fungi in Korea: Cunninghamella bertholletiae, Cunninghamella echinulata, and Cunninghamella elegans. Mycobiology 45: 318–326. https://doi.org/10.5941/MYCO.2017.45.4.318
Nie, Y., Cai, Y., Gao, Y., Yu, D.S., Wang, Z.M., Liu, X.Y. & Huang, B. (2020a) Three new species of Conidiobolus sensu stricto from plant debris in eastern China. MycoKeys 73: 133–149. https://doi.org/10.3897/mycokeys.73.56905
Nie, Y., Yu, D.S., Wang, C.F., Liu, X.Y. & Huang, B. (2020b) A taxonomic revision of the genus Conidiobolus (Ancylistaceae, Entomophthorales): four clades including three new genera. MycoKeys 66: 55–81. https://doi.org/10.3897/mycokeys.66.46575
Posada, D. & Crandall, K.A. (1998) ModelTest: testing the model of DNA substitution. Bioinformatics 14: 817–818. https://doi.org/10.1093/bioinformatics/14.9.817
Ronquist, F., Teslenko, M., Van Der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61: 539–542. https://doi.org/10.1093/sysbio/sys029
Spatafora, J.W., Chang, Y., Benny, G.L., Lazarus, K., Smith, M.E., Berbee, M.L., Bonito, G., Corradi, N., Grigoriev, I. & Gryganskyi, A. (2016) A phylum-level phylogenetic classification of zygomycete fungi based on genome-scale data. Mycologia 108: 1028–1046. https://doi.org/10.3852/16-042
Stamatakis, A. (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30: 1312–1313. https://doi.org/10.1093/bioinformatics/btu033
Suwannarach, N., Kumla, J., Supo, C., Honda, Y., Nakazawa, T., Khanongnuch, C. & Wongputtisin, P. (2021) Cunninghamella saisamornae (Cunninghamellaceae, Mucorales), a new soil fungus from northern Thailand. Phytotaxa 509: 291–300. https://doi.org/10.11646/phytotaxa.509.3.4
Swofford, D.L. (2002) PAUP*: Phylogenetic Analysis Using Parsimony (* and Other Methods); Version 4.0b10; Sinauer Associates: Sunderland, MA, USA.
Voigt, K., James, T.Y., Kirk, P.M., da Santiago, A.L.C.M., Waldman, B., Griffith, G.W., Fu, M., Radek, R., Strassert, J.F.H., Wurzbacher, C., Jerônimo, G.H., Simmons, D.R., Seto, K., Gentekaki, E., Hurdeal, V.G., Hyde, K.D., Nguyen, T.T.T. & Lee, H.B. (2021) Early-diverging fungal phyla: taxonomy, species concept, ecology, distribution, anthropogenic impact, and novel phylogenetic proposals. Fungal Diversity 109: 59–98. https://doi.org/10.1007/s13225-021-00480-y
Walther, G., Pawłowska, J., Alastruey-Izquierdo, A., Wrzosek, M., Rodriguez-Tudela, J.L., Dolatabadi, S., Chakrabarti, A. & de Hoog, G.S. (2013) DNA barcoding in Mucorales: an inventory of biodiversity. Persoonia 30: 11–47. https://doi.org/10.3767/003158513X665070
Walther, G., Wagner, L. & Kurzai, O. (2019) Updates on the taxonomy of Mucorales with an emphasis on clinically important taxa. Journal of Fungi 5: 106. https://doi.org/10.3390/jof5040106
Wang, K., Dai, Y.C., Jia, Z.F., Li, T.H., Liu, T.Z., Dorji, P., Reyim, M., Sun, G.Y., Tolgor, B., Wei, S.L., Yang, Z.L., Yuan, H.S., Zhang, X.G. & Cai, L. (2021) Overview of China’s nomenclature novelties of fungi in the new century (2000-2020). Mycosystema 40: 822–833. https://doi.org/10.13346/j.mycosystema.210064
Wang, K., Liu, F. & Cai, L. (2022) A name list of common agricultural phytopathogenic fungi in China. Mycosystema 41: 361–386. https://doi.org/10.13346/j.mycosystema.210483
White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenies. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR Protocols: a guide to methods and applications. Academic Press, Inc, San Diego, California, pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
Wijayawardene, N., Hyde, K., Al-Ani, L., Tedersoo, L., Haelewaters, D., Rajeshkumar, K., Zhao, R., Aptroot, A., Leontyev, D. & Saxena, R. (2020) Outline of Fungi and fungus-like taxa. Mycosphere 11: 1060–1456. https://doi.org/10.5943/mycosphere/11/1/8
Wu, F., Zhou, L.W., Josef, V. & Dai, Y.C. (2022) Global diversity and systematics of Hymenochaetaceae with poroid hymenophore. Fungal Diversity 113: 1–192. https://doi.org/10.1007/s13225-021-00496-4
Yu, J., Walther, G., van Diepeningen, A.H.G, Gerrits, V.D.E., Li, R.Y,, Moussa, T., Almaghrabi, O. & De Hoog, G.S. (2014) DNA barcoding of clinically relevant Cunninghamella species. Medical Mycology 53: 99–106. https://doi.org/10.1093/mmy/myu079
Zhang, Z.Y., Zhao, Y.X., Shen, X., Chen, W.H., Han, Y.F., Huang, J.Z. & Liang, Z.Q. (2020) Molecular phylogeny and morphology of Cunninghamella guizhouensis sp. nov. (Cunninghamellaceae, Mucorales), from soil in Guizhou, China. Phytotaxa 455: 31–39. https://doi.org/10.11646/phytotaxa.455.1.4
Zhao, H., Lv, M.L., Liu, Z., Zhang, M.Z., Wang, Y.N., Ju, X., Song, Z., Ren, L.Y., Jia, B.S., Qiao, M. & Liu, X.Y. (2021a) High-yield oleaginous fungi and high-value microbial lipid resources from Mucoromycota. BioEnergy Research 14: 1196–1206. https://doi.org/10.1007/s12155-020-10219-3
Zhao, H., Nie, Y., Zong, T.K., Dai, Y.C. & Liu, X.Y. (2022) Three new species of Absidia (Mucoromycota) from China based on phylogeny, morphology and physiology. Diversity 14: 132. https://doi.org/10.3390/d14020132
Zhao, H., Zhu, J., Zong, T.K., Liu, X.L., Ren, L.Y., Lin, Q., Qiao, M., Nie, Y., Zhang, Z.D. & Liu, X.Y. (2021b) Two new species in the family Cunninghamellaceae from China. Mycobiology 49: 142–150. https:// doi.org/10.1080/12298093.2021.1904555
Zheng, R.Y. & Chen, G.Q. (1998) Cunninghamella clavata sp. nov., a fungus with an unusual type of branching of sporophore. Mycotaxon 69: 187–198
Zheng, R.Y. & Chen, G.Q (2001) A monograph of Cunninghamella. Mycotaxon 80: 1–75
Zheng, R.Y., Chen, G.Q., Huang, H. & Liu, X.Y. (2007) A monograph of Rhizopus. Sydowia 59: 273–372
Zong, T.K., Zhao, H., Liu, X.L., Ren, L.Y., Zhao, C.L. & Liu, X.Y. (2021) Taxonomy and phylogeny of four new species in Absidia (Cunninghamellaceae, Mucorales) from China. Frontiers in Microbiology 12: 2181. https:// doi.org/10.3389/fmicb.2021.677836