Abstract
During a survey of endophytic fungi associated with ornamental plants in the Czech Republic, Paecilomyces-like strains were isolated from the root of Clematis. Analyses based on a combined internal transcribed spacer region (ITS), beta-tubulin (tub2) and calmodulin (CaM) sequence data matrix were applied to infer the phylogenetic position of these isolates. The novel species is characterized by phialides with a cylindrical basal portion tapering to a thin long neck producing pyriform conidia in chains. The new species is introduced with comprehensive descriptions, illustrations and a phylogenetic tree herein. Two primer pairs targeting the partial CaM gene, cm1F/cm1R and cm2F/cm2R, were designed in this study.
References
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Beuchat, L.R. & Rice, S.L. (1979) Byssochlamys spp. and processed fruits. Advances in Food Research 25: 237–288.
van den Brule, T., Punt, M., Teertstra, W., Houbraken, J., Wösten, H. & Dijksterhuis, J. (2020) The most heat-resistant conidia observed to date are formed by distinct strains of Paecilomyces variotii. Environmental Microbiology 22: 986–999. https://doi.org/10.1111/1462-2920.14791
Carbone, I. & Kohn, L.M. (1999) A Method for Designing Primer Sets for Speciation Studies in Filamentous Ascomycetes. Mycologia 91: 553–556. https://doi.org/10.2307/3761358
Crous, P.W., Wingfield, M.J., Richardson, D.M., Leroux, J.J., Strasberg, D., Edwards, J., Roets, F., Hubka, V., Taylor, P.W.J., Heykoop, M. & Martín, M.P. (2016) Fungal Planet description sheets: 400-468. Persoonia 36: 316–458. https://doi.org/10.3767/003158516X692185
Glass, N.L. & Donaldson, G.C. (1995) Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous Ascomycetes. Applied and Environmental Microbiology 61: 1323–1330.
Hong, S.B., Cho, H.S., Shin, H.D., Frisvad, J.C. & Samson, R.A. (2006) Novel Neosartorya species isolated from soil in Korea. International journal of systematic and evolutionary microbiology 56: 477–486. https://doi.org/10.1099/ijs.0.63980-0
Houbraken, J., Samson, R.A. & Frisvad, J.C. (2006) Byssochlamys: significance of heat resistance and mycotoxin production. Advances in Experimental Medicine and Biology: 571. Springer, Boston, MA. https://doi.org/10.1007/0-387-28391-9_14
Houbraken, J., Varga, J., Rico-Munoz, E., Johnson, S. & Samson, R.A. (2008) Sexual reproduction as the cause of heat resistance in the food spoilage fungus Byssochlamys spectabilis (anamorph Paecilomyces variotii). Applied and environmental microbiology 74: 1613–1619. https://doi.org/10.1128/AEM.01761-07
Houbraken, J., Spierenburg, H. & Frisvad, J.C. (2012) Rasamsonia, a new genus comprising thermotolerant and thermophilic Talaromyces and Geosmithia species. Antonie van Leeuwenhoek 101: 403–421. https://doi.org/10.1007/s10482-011-9647-1
Houbraken, J., Kocsubé, S., Visagie, C.M., Yilmaz, N., Wang, X., Meijer, M., Kraak, B., Hubka, V., Bensch, K., Samson, R.A. & Frisvad, J.C. (2020) Classification of Aspergillus, Penicillium, Talaromyces and related genera (Eurotiales): An overview of families, genera, subgenera, sections, series and species. Studies in Mycology 95: 5–169.
Hull, R. (1939) Study of Byssochlamys fulva and control measures in processed fruits. Annals of Applied Biology 26: 800–822.
Jensen, A.B., Aronstein, K., Flores, J.M., Vojvodic, S., Palacio, M.A. & Spivak, M. (2013) Standard methods for fungal brood disease research. Journal of apicultural research 52 (1): 1–20. https://doi.org/10.3896/IBRA.1.52.1.13
Katoh, K., Rozewicki, J. & Yamada, K.D. (2019) MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Brief Bioinform 20 (4): 1160–1166. https://doi.org/10.1093/bib/bbx108
Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. (2018) MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Molecular Biology and Evolution 35: 1547–1549. https://doi.org/10.1093/molbev/msy096
Kramer, R.K., Davis, N.D. & Diener, U.L. (1976) Byssotoxin A, a secondary metabolite of Byssochlamys fulva. Applied and Environmental Microbiology 31: 249–253.
Luangsa-Ard, J.J., Hywel-Jones, N.L. & Samson, R.A. (2004) The polyphyletic nature of Paecilomyces sensu lato based on 18S-generated rDNA phylogeny. Mycologia 96: 773–780. https://doi.org/10.1080/15572536.2005.11832925
Mori, T., Shin-ya, K., Takatori, K., Aihara, M. & Hayakawa, Y. (2003) Byssochlamysol, a new antitumor steroid against IGF-1-dependent cells from Byssochlamys nivea, II Physico-chemical properties and structure elucidation. Journal of Antibiotics 56: 6–8.
Phukhamsakda, C., McKenzie, E.H., Phillips, A.J.L, Gareth Jones, E.B., Jayarama Bhat, D., Stadler, M., Bhunjun, C.S., Wanasinghe, D.N., Thongbai, B., Camporesi, E. & Ertz, D. (2020) Microfungi associated with Clematis (Ranunculaceae) with an integrated approach to delimiting species boundaries. Fungal Diversity 102: 1–203. https://doi.org/10.1007/s13225-020-00448-4
Rayner, A.J. (1970) The demand for inputs and the aggregate supply function for agriculture. Journal of Agricultural Economics 21: 225–238. https://doi.org/10.1111/j.1477-9552.1970.tb02033.x
Rehner, S.A. & Samuels, G.J. (1994) Taxonomy and phylogeny of Gliocladium analysed from nuclear large subunit ribosomal DNA sequences. Mycological Research 98: 625–634.
Rice, S.L. (1977) Polygalacturonase, biomass, ascospore, and patulin production of Byssochhmys fulva. Ph.D. Dissertation, University of Georgia, Athens.
Samson, R.A., Hoekstra, E.S. & Frisvad, J.C. (2000) Introduction to food- and airborne fungi. 6th rev. ed. Utrecht: Centraalbureau voor schimmelcultures.
Spetik, M., Berraf-Tebbal, A., Penazova, E., Pecenka, J., Maier, M. & Eichmeier, A. (2019) First Report of Pseudonectria buxi Causing Volutella Blight on Boxwood in Czech Republic. Plant Disease 103 (7): 1790. https://doi.org/10.1094/PDIS-02-19-0258-PDN
Spetik, M., Berraf-Tebbal, A., Pokluda, R. & Eichmeier, A. (2021) Pyrenochaetopsis kuksensis (Pyrenochaetopsidaceae), a new species associated with an ornamental boxwood in the Czech Republic. Phytotaxa 498 (3): 177–185. https://doi.org/10.11646/phytotaxa.498.3
Splittstoesser, D.F. (1987) Fruits and fruit products. In: Beuchat, L.R. (ed.) Food and Beverage Mycology. AVI Van Nostrand Reinhold, New York, pp. 101–122.
Stolk, A.C. & Samson, R.A. (1971) Studies on Talaromyces and related genera I. Hamigera gen. nov. and Byssochlamys. Persoonia - Molecular Phylogeny and Evolution of Fungi 6: 341–357.
Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238–4246.
Vu, T., Groenewald, M., Vries, M.,Gehrmann, T., Stielow, B., Eberhardt, U., Al-Hatmi, A., Groenewald, J.Z. Cardinali, G., Houbraken, J., Boekhout, T., Crous, P., Robert, V. & Verkley, G.J.M. (2018) Large-scale generation and analysis of filamentous fungal DNA barcodes boosts coverage for kingdom Fungi and reveals thresholds for fungal species and higher taxon delimitation. Studies in Mycology 91: 23–36. https://doi.org/10.1016/j.simyco.2018.05.001
Westling, R. (1909) Byssochlamys nivea, en foreningslank mellam familjerna Gymnoascaceae och Endomycetaceae. Svensk Botanisk Tidskrift 3: 125–137.
Beuchat, L.R. & Rice, S.L. (1979) Byssochlamys spp. and processed fruits. Advances in Food Research 25: 237–288.
van den Brule, T., Punt, M., Teertstra, W., Houbraken, J., Wösten, H. & Dijksterhuis, J. (2020) The most heat-resistant conidia observed to date are formed by distinct strains of Paecilomyces variotii. Environmental Microbiology 22: 986–999. https://doi.org/10.1111/1462-2920.14791
Carbone, I. & Kohn, L.M. (1999) A Method for Designing Primer Sets for Speciation Studies in Filamentous Ascomycetes. Mycologia 91: 553–556. https://doi.org/10.2307/3761358
Crous, P.W., Wingfield, M.J., Richardson, D.M., Leroux, J.J., Strasberg, D., Edwards, J., Roets, F., Hubka, V., Taylor, P.W.J., Heykoop, M. & Martín, M.P. (2016) Fungal Planet description sheets: 400-468. Persoonia 36: 316–458. https://doi.org/10.3767/003158516X692185
Glass, N.L. & Donaldson, G.C. (1995) Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous Ascomycetes. Applied and Environmental Microbiology 61: 1323–1330.
Hong, S.B., Cho, H.S., Shin, H.D., Frisvad, J.C. & Samson, R.A. (2006) Novel Neosartorya species isolated from soil in Korea. International journal of systematic and evolutionary microbiology 56: 477–486. https://doi.org/10.1099/ijs.0.63980-0
Houbraken, J., Samson, R.A. & Frisvad, J.C. (2006) Byssochlamys: significance of heat resistance and mycotoxin production. Advances in Experimental Medicine and Biology: 571. Springer, Boston, MA. https://doi.org/10.1007/0-387-28391-9_14
Houbraken, J., Varga, J., Rico-Munoz, E., Johnson, S. & Samson, R.A. (2008) Sexual reproduction as the cause of heat resistance in the food spoilage fungus Byssochlamys spectabilis (anamorph Paecilomyces variotii). Applied and environmental microbiology 74: 1613–1619. https://doi.org/10.1128/AEM.01761-07
Houbraken, J., Spierenburg, H. & Frisvad, J.C. (2012) Rasamsonia, a new genus comprising thermotolerant and thermophilic Talaromyces and Geosmithia species. Antonie van Leeuwenhoek 101: 403–421. https://doi.org/10.1007/s10482-011-9647-1
Houbraken, J., Kocsubé, S., Visagie, C.M., Yilmaz, N., Wang, X., Meijer, M., Kraak, B., Hubka, V., Bensch, K., Samson, R.A. & Frisvad, J.C. (2020) Classification of Aspergillus, Penicillium, Talaromyces and related genera (Eurotiales): An overview of families, genera, subgenera, sections, series and species. Studies in Mycology 95: 5–169.
Hull, R. (1939) Study of Byssochlamys fulva and control measures in processed fruits. Annals of Applied Biology 26: 800–822.
Jensen, A.B., Aronstein, K., Flores, J.M., Vojvodic, S., Palacio, M.A. & Spivak, M. (2013) Standard methods for fungal brood disease research. Journal of apicultural research 52 (1): 1–20. https://doi.org/10.3896/IBRA.1.52.1.13
Katoh, K., Rozewicki, J. & Yamada, K.D. (2019) MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Brief Bioinform 20 (4): 1160–1166. https://doi.org/10.1093/bib/bbx108
Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. (2018) MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Molecular Biology and Evolution 35: 1547–1549. https://doi.org/10.1093/molbev/msy096
Kramer, R.K., Davis, N.D. & Diener, U.L. (1976) Byssotoxin A, a secondary metabolite of Byssochlamys fulva. Applied and Environmental Microbiology 31: 249–253.
Luangsa-Ard, J.J., Hywel-Jones, N.L. & Samson, R.A. (2004) The polyphyletic nature of Paecilomyces sensu lato based on 18S-generated rDNA phylogeny. Mycologia 96: 773–780. https://doi.org/10.1080/15572536.2005.11832925
Mori, T., Shin-ya, K., Takatori, K., Aihara, M. & Hayakawa, Y. (2003) Byssochlamysol, a new antitumor steroid against IGF-1-dependent cells from Byssochlamys nivea, II Physico-chemical properties and structure elucidation. Journal of Antibiotics 56: 6–8.
Phukhamsakda, C., McKenzie, E.H., Phillips, A.J.L, Gareth Jones, E.B., Jayarama Bhat, D., Stadler, M., Bhunjun, C.S., Wanasinghe, D.N., Thongbai, B., Camporesi, E. & Ertz, D. (2020) Microfungi associated with Clematis (Ranunculaceae) with an integrated approach to delimiting species boundaries. Fungal Diversity 102: 1–203. https://doi.org/10.1007/s13225-020-00448-4
Rayner, A.J. (1970) The demand for inputs and the aggregate supply function for agriculture. Journal of Agricultural Economics 21: 225–238. https://doi.org/10.1111/j.1477-9552.1970.tb02033.x
Rehner, S.A. & Samuels, G.J. (1994) Taxonomy and phylogeny of Gliocladium analysed from nuclear large subunit ribosomal DNA sequences. Mycological Research 98: 625–634.
Rice, S.L. (1977) Polygalacturonase, biomass, ascospore, and patulin production of Byssochhmys fulva. Ph.D. Dissertation, University of Georgia, Athens.
Samson, R.A., Hoekstra, E.S. & Frisvad, J.C. (2000) Introduction to food- and airborne fungi. 6th rev. ed. Utrecht: Centraalbureau voor schimmelcultures.
Spetik, M., Berraf-Tebbal, A., Penazova, E., Pecenka, J., Maier, M. & Eichmeier, A. (2019) First Report of Pseudonectria buxi Causing Volutella Blight on Boxwood in Czech Republic. Plant Disease 103 (7): 1790. https://doi.org/10.1094/PDIS-02-19-0258-PDN
Spetik, M., Berraf-Tebbal, A., Pokluda, R. & Eichmeier, A. (2021) Pyrenochaetopsis kuksensis (Pyrenochaetopsidaceae), a new species associated with an ornamental boxwood in the Czech Republic. Phytotaxa 498 (3): 177–185. https://doi.org/10.11646/phytotaxa.498.3
Splittstoesser, D.F. (1987) Fruits and fruit products. In: Beuchat, L.R. (ed.) Food and Beverage Mycology. AVI Van Nostrand Reinhold, New York, pp. 101–122.
Stolk, A.C. & Samson, R.A. (1971) Studies on Talaromyces and related genera I. Hamigera gen. nov. and Byssochlamys. Persoonia - Molecular Phylogeny and Evolution of Fungi 6: 341–357.
Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238–4246.
Vu, T., Groenewald, M., Vries, M.,Gehrmann, T., Stielow, B., Eberhardt, U., Al-Hatmi, A., Groenewald, J.Z. Cardinali, G., Houbraken, J., Boekhout, T., Crous, P., Robert, V. & Verkley, G.J.M. (2018) Large-scale generation and analysis of filamentous fungal DNA barcodes boosts coverage for kingdom Fungi and reveals thresholds for fungal species and higher taxon delimitation. Studies in Mycology 91: 23–36. https://doi.org/10.1016/j.simyco.2018.05.001
Westling, R. (1909) Byssochlamys nivea, en foreningslank mellam familjerna Gymnoascaceae och Endomycetaceae. Svensk Botanisk Tidskrift 3: 125–137.