Abstract
A fungus within the family Dictyosporiaceae was isolated from submerged-decaying wood in Sichuan Province, China. Combined ITS, LSU, SSU and tef1 sequence data showed that the species represented a new genus and is described here as Neodigitodesmium cheirosporum gen. et sp. nov. Neodigitodesmium distinct from other genera in the family Dictyosporiaceae based on a hyaline and holoblastic basal connecting conidiogenous cell, conical shaped and smaller with fewer rows (1–4) of conidia. In addition, phylogenetic analysis and morphological comparison showed that the recently introduced genus Paradictyocheirospora is congeneric with Digitodesmium. Based on these results, Paradictyocheirospora is reduced to synonymy under the older generic name Digitodesmium.
References
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Liang, Z.Q., Wang, B. & Kang, J.C. (2003) Several rare entopathogenic fungi from the Western Sichuan mountains. Fungal Diversity 12: 129–134.
Meireles, V.C., Labegalini, C.M.G. & Baldissera, V.D.A. (2019) Método Tracer e a qualidade do cuidado na enfermagem: revisão integrativa da literatura. Revista Gaúcha de Enfermagem 40 (2): e20180142. https://doi.org/10.1590/1983-1447.2019.20180142
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Paradis, E., Claude, J. & Strimmer, K. (2004) APE: Analyses of Phylogenetics and Evolution in R language. Bioinformatics 20 (2): 289–290. https://doi.org/10.1093/bioinformatics/btg412
Rehner, S.A. & Buckley, E. (2005) A Beauveria phylogeny inferred from nuclear ITS and EF1-alpha sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 97 (1): 84–98. https://doi.org/10.3852/mycologia.97.1.84
Senanayake, I.C, Rathnayaka, A.R., Sandamali, D.S., Calabon, M.S., Gentekaki, E., Lee, H.B., Hurdeal, V.G., Pem, D., Dissanayake, L.S., Wijesinghe, S.N., Bundhun, D., Nguyen, T.T., Goonasekara, I.D., Abeywickrama, P.D., Jayawardena, R.S., Wanasinghe, D.N., Jeewon, R., Bhat, D.J. & Xiang, M.M. (2020) Morphological approaches in studying fungi: collection, examination, isolation, sporulation and preservation. Mycosphere 11: 2678–2754. https://doi.org/10.5943/mycosphere/11/1/20
Toju, H., Tanabe, A.S., Yamamoto, S. & Sato, H. (2012) High-Coverage ITS Primers for the DNA-Based Identification of Ascomycetes and Basidiomycetes in Environmental Samples. Plos One 7 (7): e40863. https://doi.org/10.1371/journal.pone.0040863
Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172 (8): 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
Wang, R.X., Luo, Z.L., Hyde, K.D., Bhat, J.D., Su, X.J. & Su, H.Y. (2016) New species and records of Dictyocheirospora from submerged wood in north-western Yunnan, China. Mycosphere 7 (9): 1357–1367. https://doi.org/10.5943/mycosphere/7/9/9
Yan, K., Abbas, M., Meng, L., Cai, H., Peng, Z., Li, Q., El-Sappah, A.H., Yan, L. & Zhao, X. (2021) Analysis of the Fungal Diversity and Community Structure in Sichuan Dark Tea During Pile-Fermentation. Frontiers in Microbiology 12: 706714 https://doi.org/10.3389/fmicb.2021.706714
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Yu, G. (2020) Using ggtree to Visualize Data on Tree-Like Structures. Current Protocols in Bioinformatics 69 (1): e96. https://doi.org/10.1002/cpbi.96
You, Z., He, S., Gong, G., Zhang, S., Chang, X.L., Liu, N., Sun, X., Qi, X., Ye, K. & Wang, Y. (2014) Soil fungal diversity in three nature reserves of Jiuzhaigou County, Sichuan Province, China. Annals of Microbiology 64: 1275–1290. https://doi.org/10.1007/s13213-013-0772-0
Zhou, W., Wei, Q., Feng, R., Liu, Y., Liang, H., Li, J. & Yan, K. (2021) Diversity and spatial distribution of endophytic fungi in Cinnamomum longepaniculatum of Yibin, China. Archives of Microbiology 203: 3361–3372 https://doi.org/10.1007/s00203-021-02325-3
Atienza, V., Hawksworth, D.L. & Pérez, O.S. (2021) Verrucoccum (Dothideomycetes, Dictyosporiaceae), a new genus of lichenicolous fungi on Lobaria s. lat. for the Dothidea hymeniicola species complex. Mycologia 113 (6): 1233–1252. https://doi.org/10.1080/00275514.2021.1966281
Boonmee, S., D’souza, M.J., Luo, Z.L., Pinruan, U., Tanaka, K., Su, H., Bhat, D.J., McKenzie, E.H.C., Jones, E.B.G., Taylor, J.E., Phillips, A.J.L., Hirayama, K., Eungwanichayapant, P.D. & Hyde, K.D. (2016) Dictyosporiaceae fam. nov. Fungal Diversity 80: 457–482. https://doi.org/10.1007/s13225-016-0363-z
Bouckaert, R., Vaughan, T.G., Barido-Sottani, J., Duchene, S., Fourment, M., Gavryushkina, A., Heled, J., Jones, G., Kühnert, D., De Maio, N., Matschiner, M., Mendes, F.K., Müller, N.F., Ogilvie, H.A., Plessis, L., Popinga, A., Rambaut, A., Rasmussen, D., Siveroni, I., Suchard, M.A., Wu, C., Xie, D., Zhang, C., Stadler, T. & Drummond, A.J. (2019) BEAST 2.5: An Advanced Software Platform for Bayesian Evolutionary Analysis. PLOS Computational Biology 15: e1006650. https://doi.org/10.1371/journal.pcbi.1006650
Capella-Gutiérrez, S., Silla-Martínez, J.M. & Gabaldón, T. (2009) trimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses. Bioinformatics 25 (15): 1972–1973. https://doi.org/10.1093/bioinformatics/btp348
Carbone, I. & Kohn, L.M. (1999) A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91 (3): 553–556. https://doi.org/10.1080/00275514.1999.12061051
Corda, A.C. (1836) Mykologische Beobachtungen. Weitenweber’s Beitrage zur gesammtem Natur-und Heilwissenschaften Prague.
Egger, K.N. (1995) Molecular analysis of ectomycorrhizal fungal communities. Canadian Journal of Botany 73 (S1): 1415–1422. https://doi.org/10.1139/b95-405
Hibbett, D.S. (1996) Phylogenetic evidence for horizontal transmission of group I introns in the nuclear ribosomal DNA of mushroom-forming fungi. Molecular Biology and Evolution 13 (7): 903–917. https://doi.org/10.1093/oxfordjournals.molbev.a025658
Huelsenbeck, J.P. & Ronquist, F. (2000) MrBayes: A Program for the Bayesian Inference of Phylogeny. Bioinformatics 17: 754–755. https://doi.org/10.1093/bioinformatics/17.8.754
Rajeshkumar, K.C., Verma, R.K., Boonmee, S., Chandrasiri, S., Hyde, K.D., Ashtekar, N., Lad, S. & Wijayawardene, N. (2021) Paradictyocheirospora tectonae, a novel genus in the family Dictyosporiaceae from India. Phytotaxa 509 (3): 259–271 https://doi.org/10.11646/phytotaxa.509.3.1
Rehner, S.A. & Buckley, E. (2005) A Beauveria phylogeny inferred from nuclear ITS and EF1-alpha sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 97: 84–98. https://doi.org/10.3852/mycologia.97.1.84.
Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30 (4): 772–780. https://doi.org/10.1093/molbev/mst010
Kirk, P.M. (1981) New or interesting microfungi II. Dematiaceous hyphomycetes from Esher Common, Surrey. Transactions of the British Mycological Society 77 (2): 279–297. https://doi.org/10.1016/S0007-1536(81)80031-9
Lanfear, R., Frandsen, P.B., Wright, A.M., Senfeld, T. & Calcott, B. (2016) PartitionFinder 2: New Methods for Selecting Partitioned Models of Evolution for Molecular and Morphological Phylogenetic Analyses. Molecular Biology and Evolution 34 (3): 772–773. https://doi.org/10.1093/molbev/msw260
Li, W.L., Luo, Z.L., Liu, J.K., Bhat, D.J., Bao, D.F., Su, H.Y. & Hyde, K.D. (2017) Lignicolous freshwater fungi from China I : Aquadictyospora lignicola gen. et sp. nov. and new record of Pseudodictyosporium wauense from northwestern Yunnan Province. Mycosphere 8: 1587–1597. https://doi.org/10.5943/mycosphere/8/10/1
Liang, Z.Q., Wang, B. & Kang, J.C. (2003) Several rare entopathogenic fungi from the Western Sichuan mountains. Fungal Diversity 12: 129–134.
Meireles, V.C., Labegalini, C.M.G. & Baldissera, V.D.A. (2019) Método Tracer e a qualidade do cuidado na enfermagem: revisão integrativa da literatura. Revista Gaúcha de Enfermagem 40 (2): e20180142. https://doi.org/10.1590/1983-1447.2019.20180142
Nguyen, L.T., Schmidt, H.A., von Haeseler, A. & Minh, B.Q. (2015) IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular Biology and Evolution 32 (1): 268–274. https://doi.org/10.1093/molbev/msu300
Nóbrega, T., Ferreira, B.W. & Barreto, R.W. (2021) Digitodesmium polybrachiatum sp. nov., a new species of Dictyosporiaceae from Brazil. Mycological Progress 20: 1135–1144. https://doi.org/10.1007/s11557-021-01717-5
Paradis, E., Claude, J. & Strimmer, K. (2004) APE: Analyses of Phylogenetics and Evolution in R language. Bioinformatics 20 (2): 289–290. https://doi.org/10.1093/bioinformatics/btg412
Rehner, S.A. & Buckley, E. (2005) A Beauveria phylogeny inferred from nuclear ITS and EF1-alpha sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 97 (1): 84–98. https://doi.org/10.3852/mycologia.97.1.84
Senanayake, I.C, Rathnayaka, A.R., Sandamali, D.S., Calabon, M.S., Gentekaki, E., Lee, H.B., Hurdeal, V.G., Pem, D., Dissanayake, L.S., Wijesinghe, S.N., Bundhun, D., Nguyen, T.T., Goonasekara, I.D., Abeywickrama, P.D., Jayawardena, R.S., Wanasinghe, D.N., Jeewon, R., Bhat, D.J. & Xiang, M.M. (2020) Morphological approaches in studying fungi: collection, examination, isolation, sporulation and preservation. Mycosphere 11: 2678–2754. https://doi.org/10.5943/mycosphere/11/1/20
Toju, H., Tanabe, A.S., Yamamoto, S. & Sato, H. (2012) High-Coverage ITS Primers for the DNA-Based Identification of Ascomycetes and Basidiomycetes in Environmental Samples. Plos One 7 (7): e40863. https://doi.org/10.1371/journal.pone.0040863
Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172 (8): 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
Wang, R.X., Luo, Z.L., Hyde, K.D., Bhat, J.D., Su, X.J. & Su, H.Y. (2016) New species and records of Dictyocheirospora from submerged wood in north-western Yunnan, China. Mycosphere 7 (9): 1357–1367. https://doi.org/10.5943/mycosphere/7/9/9
Yan, K., Abbas, M., Meng, L., Cai, H., Peng, Z., Li, Q., El-Sappah, A.H., Yan, L. & Zhao, X. (2021) Analysis of the Fungal Diversity and Community Structure in Sichuan Dark Tea During Pile-Fermentation. Frontiers in Microbiology 12: 706714 https://doi.org/10.3389/fmicb.2021.706714
Yang, J., Liu, J.K., Hyde, K.D., Jones, E.B.G. & Liu, Z.Y. (2018) New species in Dictyosporium, new combinations in Dictyocheirospora and an updated backbone tree for Dothideomycetes. MycoKeys 36: 83–105. https://doi.org/10.3897/mycokeys.36.27051
Yu, G. (2020) Using ggtree to Visualize Data on Tree-Like Structures. Current Protocols in Bioinformatics 69 (1): e96. https://doi.org/10.1002/cpbi.96
You, Z., He, S., Gong, G., Zhang, S., Chang, X.L., Liu, N., Sun, X., Qi, X., Ye, K. & Wang, Y. (2014) Soil fungal diversity in three nature reserves of Jiuzhaigou County, Sichuan Province, China. Annals of Microbiology 64: 1275–1290. https://doi.org/10.1007/s13213-013-0772-0
Zhou, W., Wei, Q., Feng, R., Liu, Y., Liang, H., Li, J. & Yan, K. (2021) Diversity and spatial distribution of endophytic fungi in Cinnamomum longepaniculatum of Yibin, China. Archives of Microbiology 203: 3361–3372 https://doi.org/10.1007/s00203-021-02325-3