Abstract
Hydnobolites oaxacanus is described using molecular and morphological data. This new species is characterized by the whitish ascomata with orange spots, grey gleba, and 4-spored asci with ascospores of 17–45 × 15–20 µm. The new species differs from other American Hydnobolites such as H. californicum and H. cerebriformis by the number of ascospores per asci, and larger ascospore size and ornamentation. It is putatively associated with Quercus castanea in the Quercus-Pinus dry mixed forest. Photographs, descriptions, and molecular phylogeny of the ITS region are presented.
References
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Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95?98.
Healy, R.A., Smith, M.E., Bonito, G.M., Pfister, D.H, Ge, Z.W., Guevara, G., Williams, G., Stafford, K., Kumar, L., Lee, T., Hobart, C., Trappe, J., Vilgalys, R. & McLaughlin, D.J. (2013) High diversity and widespread occurrence of mitotic spore mats in ectomycorrhizal Pezizales. Molecular Ecology 22 (6): 1717–1732. https://doi.org/10.1111/mec.12135
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Kornerup, A. & Wanscher, H. (1978) Methuen handbook of colour. 3rd edition. Eyre, London, 227 pp.
Lang, C., Seven, J. & Polle, A. (2011) Host preferences and differential contributions of deciduous tree species shape mycorrhizal species richness in a mixed Central European Forest. Mycorrhiza 21 (4): 297–308. https://doi.org/10.1007/s00572-010-0338-y.
Lanfear, R., Calcott, B., Kainer, D., Mayer, C. & Stamatakis, A. (2014) Selecting optimal partitioning schemes for phylogenomic datasets. BMC Evolutionary Biology 14: 1?14. https://doi.org/10.1186/1471-2148-14-82
Lanfear, R., Frandsen, P.B., Wright, A.M., Senfeld, T. & Calcott, B. (2016) PartitionFinder 2: New methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses. Molecular Biology and Evolution 34 (3): 772?773. https://doi:10.1093/molbev/msw260
Li, T., Cheng, M., Xu, Y-Y. & Fan, L. (2019) Four new species of Hydnobolites (sequestrate Pezizaceae, Ascomycota) from China. Mycological progress 18: 405–414. https://doi.org/10.1007/s11557-018-01465-z
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Long, D., Liu, J., Han, Q., Wang, X. & Huang, J. (2016) Ectomycorrhizal fungal communities associated with Populus simonii and Pinus tabuliformis in the hilly-gully region of the Loess Plateau, China. Scientific Reports 6: 24336. https://doi.org/10.1038/srep24336
Martínez-González, C.R., Ramírez-Mendoza, R., Jiménez-Ramírez, J., Gallegos-Vázquez, C. & Luna-Vega, I. (2017) Improved method for genomic DNA extraction for Opuntia Mill. (Cactaceae). Plant Methods 13: 1?10. https://doi.org/10.1186/s13007-017-0234-y
Müller, K., Quandt, D., Müller, J. & Neinhuis, C. (2005) PhyDE®-Phylogenetic data editor. Program distributed by the authors, versión 10.0. Available from: https://www.phyde.de (accessed 22 November 2021).
Pegler, D.N., Spooner, B.M. & Young, T.W.K. (1993) British truffles. A revision of British hypogeous fungi. The Board of Trustees of the Royal Botanical Gardens, Kew, 215 pp.
Rambaut, A., Suchard, M.A., Xie, D. & Drummond, A.J. (2014) Tracer v1.6. Available from: http://beast.bio.ed.ac.uk/Tracer (accessed 22 November 2021).
Raymundo, T., Valenzuela, R., Ramírez-Martínez, J.C., Martínez-Pineda, M., Cobos-Villagrán, A. Trejo-Arana, A., Sánchez-Flores, M., Gay-González, A.D. & Luna-Vega, I. (2020) New records of Ascomycetes from the Tropical Montane Cloud Forest of eastern Mexico. Phytotaxa 454 (3): 161–185. https://doi.org/10.11646/phytotaxa.454.3.1
Roy, M., Rochet, J., Manzi, S., Jargeat, P., Gryta, H., Moreau, P.A. & Gardes, M. (2013) What determines Alnus-associated ectomycorrhizal community diversity and specificity? A comparison of host and habitat effects at a regional scale. New Phytologist 198 (4): 1228–1238. https://doi.org/10.1111/nph.12212
Sánchez-Flores, M., Valenzuela, R., Hernández-Muñoz, M.A., García-Jiménez, J., Martínez-Pineda, M. & Raymundo, T. (2020) Ascomicetos del bosque mesófilo de montaña de Honey, Puebla de los Ángeles, México. Acta Botanica Mexicana 127: e1719. https://doi.org/10.21829.2020.1719
Smith, M.E. & Schmull, M. (2011) Tropical truffles: English translation and critical review of F. von Höhnel’s truffles from Java. Mycological Progress 10 (2): 249–260. https://doi.org/10.1007/s11557-010-0694-1
Stamatakis, A. (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30: 1312–1313. https://doi.org/10.1093/bioinformatics/btu033
Tedersoo, L., Mett, M., Ishida, T.A. & Bahram, M. (2013) Phylogenetic relationships among host plants explain differences in fungal species richness and community composition in ectomycorrhizal symbiosis. New Phytologist 199 (3): 822–831.
Trappe, M., Evans, F. & Trappe, J. (2007) Field Guide to North American Truffles: Hunting, Identifying, and Enjoying the World’s Most Prized Fungi. Ten Speed Press, California. 144 pp.
Trappe, J.M., Molina, R., Luoma, D.L., Cázares, E., Pilz, D., Smith, J.E., Castellano, M.A., Miller, L. & Trappe, J.M. (2009) Diversity, ecology and conservation of the truffle fungi in forests of the pacific northwest. US Dept. of Agriculture, Forest Service General Technical Report, PNW-GTR-772, Portland 194 pp.
Tulasne, R.L. & Tulasne, C. (1843) Annales des sciences naturelles. Botanique 19: 278.
Tulasne, R.L. & Tulasne, C. (1851) Fungi hypogaei. Histoire et monographie des champignons hypogés. Paris. 222 pp.
Vittadini, C. (1831) Monographia tuberacearum. Mediolani, 88 pp.
White, T.J., Bruns, T.D., Lee, S. & Taylor, J.W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J. & White, T.J. (Eds.) PCR protocols: a guide to methods and applications. Academic, San Diego, pp. 315–322.
Wu, E., Lan, Z., Xiang, Y. & Han, Z. (1993) A new species of Tuberales from China. Mycological research 97 (8): 1021–1022.
Zhang, Z., Schwartz, S., Wagner, L. & Miller, W. (2000) A greedy algorithm for aligning DNA sequences. Journal of Computational Biology 7: 203?214.
Bahram, M., Polme, S., Koljalg, U., Zarre, S. & Tedersoo, L. (2012) Regional and local patterns of ectomycorrhizal fungal diversity and community structure along an altitudinal gradient in the Hyrcanian forests of northern Iran. New Phytologist 193 (2): 465–473. https://doi.org/10.1111/j.1469-8137.2011.03927.x.
Brock, P.M., Doring, H. & Bidartondo, M.I. (2009) How to know unknown fungi: the role of a herbarium. New Phytologist 181 (3): 719–724. https://doi.org/10.1111/j.1469-8137.2008.02703.x
Castellano, M.A., Trappe, J.M. & Luoma, D.L. (2004) Sequestrate Fungi. In: Mueller, G.M., Bills, G.F. & Foster, M.S. (eds.) Biodiversity of fungi. Inventory and monitoring methods. Elsevier Academic Press, San Diego, pp. 197–213.
Castellano, M.A., Trappe, J.M., Maser, Z. & Maser, S. (1986) Key to spores of the genera of hypogeous fungi of North America, with reference to animal mycophagy. Mad River Press, Eureka, 185 pp.
Cázares, E., García, J., Castillo, J. & Trappe, J.M. (1992) Hypogeous fungi from northern Mexico. Mycologia 84: 341–359. https://doi.org/10.2307/3760186
Corda, A.K.L. (1854) Iconum fungorum hucusque cognitorum. Prague. 91 pp.
Erlandson, S.R., Savage, J.A., Cavender-Bares, J.M. & Peay, K.G. (2016) Soil moisture and chemistry influence diversity of ectomycorrhizal fungal communities associating with willow along an hydrologic gradient. FEMS Microbiology Ecology 92 (1): fiv148. https://doi.org/10.1093/femsec/fiv148
Fisch, E. (1938) Die Natürlichen Pflanzenfamilien. Leipzig. 462 pp.
Fisch, E. (1905) Repertorium specierum novarum regni vegetabilis. Berlin. 176 pp.
Frandsen, P.B., Calcott, B., Mayer, C. & Lanfear, R. (2015) Automatic selection of partitioning schemes for phylogenetic analyses using iterative k-means clustering of site rates. BMC Evolutionary Biology 15: 1?17. https://doi.org/10.1186/s12862-015-0283-7
Gómez-Reyes, V.M., Vásquez-Marrufo, G., Ortega-Gómez, A.M. & Guevara-Guerrero, G. (2018) Ascomicetos hipogeos de la región occidental del Sistema Volcánico Transversal, México. Acta Botánica Mexicana 125: 37–48. https://doi.org/10.21829/abm125.2018.1327
Gilkey, H.M. (1916) A revision of the Tuberales of California. University of California Publications in Botany 6: 275–356.
Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95?98.
Healy, R.A., Smith, M.E., Bonito, G.M., Pfister, D.H, Ge, Z.W., Guevara, G., Williams, G., Stafford, K., Kumar, L., Lee, T., Hobart, C., Trappe, J., Vilgalys, R. & McLaughlin, D.J. (2013) High diversity and widespread occurrence of mitotic spore mats in ectomycorrhizal Pezizales. Molecular Ecology 22 (6): 1717–1732. https://doi.org/10.1111/mec.12135
Huelsenbeck, J.P. & Ronquist, F. (2001) MrBayes: Bayesian inference of phylogeny. Bioinformatics 17 (8): 754?755. https://doi.org/10.1093/bioinformatics/17.8.754
Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30 (4): 772?780. https://doi.org/10.1093/molbev/mst010
Katoh, K., Misawa, K., Kuma, K. & Miyata, T. (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Research 30 (14): 3059–3066. https://doi.org/10.1093/nar/gkf436
Katoh, K., Rozewicki, J. & Yamada, K.D. (2017) MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics 20 (4): 1160?1166. https://doi.org/10.1093/bib/bbx108
Kornerup, A. & Wanscher, H. (1978) Methuen handbook of colour. 3rd edition. Eyre, London, 227 pp.
Lang, C., Seven, J. & Polle, A. (2011) Host preferences and differential contributions of deciduous tree species shape mycorrhizal species richness in a mixed Central European Forest. Mycorrhiza 21 (4): 297–308. https://doi.org/10.1007/s00572-010-0338-y.
Lanfear, R., Calcott, B., Kainer, D., Mayer, C. & Stamatakis, A. (2014) Selecting optimal partitioning schemes for phylogenomic datasets. BMC Evolutionary Biology 14: 1?14. https://doi.org/10.1186/1471-2148-14-82
Lanfear, R., Frandsen, P.B., Wright, A.M., Senfeld, T. & Calcott, B. (2016) PartitionFinder 2: New methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses. Molecular Biology and Evolution 34 (3): 772?773. https://doi:10.1093/molbev/msw260
Li, T., Cheng, M., Xu, Y-Y. & Fan, L. (2019) Four new species of Hydnobolites (sequestrate Pezizaceae, Ascomycota) from China. Mycological progress 18: 405–414. https://doi.org/10.1007/s11557-018-01465-z
Leonardi, M., Iotti, M., Oddis, M., Lalli, G., Pacioni, G., Leonardi, P., Maccherini, S., Perini, C., Salerni, E. & Zambonelli, A. (2013) Assessment of ectomycorrhizal fungal communities in the natural habitats of Tuber magnatum (Ascomycota, Pezizales). Mycorrhiza 23 (5): 349–358. https://doi.org/10.1007/s00572-012-0474-7
Long, D., Liu, J., Han, Q., Wang, X. & Huang, J. (2016) Ectomycorrhizal fungal communities associated with Populus simonii and Pinus tabuliformis in the hilly-gully region of the Loess Plateau, China. Scientific Reports 6: 24336. https://doi.org/10.1038/srep24336
Martínez-González, C.R., Ramírez-Mendoza, R., Jiménez-Ramírez, J., Gallegos-Vázquez, C. & Luna-Vega, I. (2017) Improved method for genomic DNA extraction for Opuntia Mill. (Cactaceae). Plant Methods 13: 1?10. https://doi.org/10.1186/s13007-017-0234-y
Müller, K., Quandt, D., Müller, J. & Neinhuis, C. (2005) PhyDE®-Phylogenetic data editor. Program distributed by the authors, versión 10.0. Available from: https://www.phyde.de (accessed 22 November 2021).
Pegler, D.N., Spooner, B.M. & Young, T.W.K. (1993) British truffles. A revision of British hypogeous fungi. The Board of Trustees of the Royal Botanical Gardens, Kew, 215 pp.
Rambaut, A., Suchard, M.A., Xie, D. & Drummond, A.J. (2014) Tracer v1.6. Available from: http://beast.bio.ed.ac.uk/Tracer (accessed 22 November 2021).
Raymundo, T., Valenzuela, R., Ramírez-Martínez, J.C., Martínez-Pineda, M., Cobos-Villagrán, A. Trejo-Arana, A., Sánchez-Flores, M., Gay-González, A.D. & Luna-Vega, I. (2020) New records of Ascomycetes from the Tropical Montane Cloud Forest of eastern Mexico. Phytotaxa 454 (3): 161–185. https://doi.org/10.11646/phytotaxa.454.3.1
Roy, M., Rochet, J., Manzi, S., Jargeat, P., Gryta, H., Moreau, P.A. & Gardes, M. (2013) What determines Alnus-associated ectomycorrhizal community diversity and specificity? A comparison of host and habitat effects at a regional scale. New Phytologist 198 (4): 1228–1238. https://doi.org/10.1111/nph.12212
Sánchez-Flores, M., Valenzuela, R., Hernández-Muñoz, M.A., García-Jiménez, J., Martínez-Pineda, M. & Raymundo, T. (2020) Ascomicetos del bosque mesófilo de montaña de Honey, Puebla de los Ángeles, México. Acta Botanica Mexicana 127: e1719. https://doi.org/10.21829.2020.1719
Smith, M.E. & Schmull, M. (2011) Tropical truffles: English translation and critical review of F. von Höhnel’s truffles from Java. Mycological Progress 10 (2): 249–260. https://doi.org/10.1007/s11557-010-0694-1
Stamatakis, A. (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30: 1312–1313. https://doi.org/10.1093/bioinformatics/btu033
Tedersoo, L., Mett, M., Ishida, T.A. & Bahram, M. (2013) Phylogenetic relationships among host plants explain differences in fungal species richness and community composition in ectomycorrhizal symbiosis. New Phytologist 199 (3): 822–831.
Trappe, M., Evans, F. & Trappe, J. (2007) Field Guide to North American Truffles: Hunting, Identifying, and Enjoying the World’s Most Prized Fungi. Ten Speed Press, California. 144 pp.
Trappe, J.M., Molina, R., Luoma, D.L., Cázares, E., Pilz, D., Smith, J.E., Castellano, M.A., Miller, L. & Trappe, J.M. (2009) Diversity, ecology and conservation of the truffle fungi in forests of the pacific northwest. US Dept. of Agriculture, Forest Service General Technical Report, PNW-GTR-772, Portland 194 pp.
Tulasne, R.L. & Tulasne, C. (1843) Annales des sciences naturelles. Botanique 19: 278.
Tulasne, R.L. & Tulasne, C. (1851) Fungi hypogaei. Histoire et monographie des champignons hypogés. Paris. 222 pp.
Vittadini, C. (1831) Monographia tuberacearum. Mediolani, 88 pp.
White, T.J., Bruns, T.D., Lee, S. & Taylor, J.W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J. & White, T.J. (Eds.) PCR protocols: a guide to methods and applications. Academic, San Diego, pp. 315–322.
Wu, E., Lan, Z., Xiang, Y. & Han, Z. (1993) A new species of Tuberales from China. Mycological research 97 (8): 1021–1022.
Zhang, Z., Schwartz, S., Wagner, L. & Miller, W. (2000) A greedy algorithm for aligning DNA sequences. Journal of Computational Biology 7: 203?214.