Abstract
A new species of Acrocalymma, A. guizhouense, isolated from the rhizosphere soil of Perilla frutescens, is introduced. Morphological characteristics, culture characteristics on PDA, OA, MEA, and phylogenetic analyses based on multi-locus datasets (SSU + LSU + ITS) support the establishment of the new species. Morphologically, A. guizhouense is distinguished from other species of Acrocalymma in having narrow conidia with a width of 1.5-2.5 µm. Phylogenetically, A. guizhouense formed a separated subclade with well-supported (100 MLBS/1.00 BYPP) value, which confirmed the taxonomic placement in the genus Acrocalymma.
References
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Dong, W., Wang, B., Hyde, K.D., McKenzie, E.H., Raja, H.A., Tanaka, K., Abdel-Wahab, M.A., Abdel-Aziz, F.A., Doilom, M., Phookamsak, R., Hongsanan, S., Wanasinghe, D.N., Yu, X., Wang, G., Yang, H., Yang, J., Thambugala, K.M., Tian, Q., Luo, Z., Yang, J., Miller, A.N., Fournier, J., Boonmee, S., Hu, D., Nalumpang, S. & Zhang, H. (2020) Freshwater Dothideomycetes. Fungal Diversity 105: 319–575. https://doi.org/10.1007/s13225-020-00463-5
Irwin, J.A.G. (1972) Stagonospora root and crown rot of lucerne. Australasian Plant Pathology 1 (4): 29–30. https://doi.org/10.1071/app9720029
Jayasiri, S.C., Hyde, K.D., Jones, E.B.G., McKenzie, E., Jeewon, R., Phillips, A.J.L., Bhat, D.J., Wanasinghe, D.N., Liu, J.J., Lu, Y., Kang, J., Xu, J. & Karunarathna, S.C. (2019) Diversity, morphology and molecular phylogeny of Dothideomycetes on decaying wild seed pods and fruits. Mycosphere 10 (1): 1–186. https:// doi.org/10.5943/mycosphere/10/1/1
Kalyaanamoorthy, S., Minh, B.Q., Wong, T.K.F., von Haeseler, A. & Jermiin, L.S. (2017) ModelFinder: fast model selection for accurate phylogenetic estimates. Nature Methods 14: 587–589. https://doi.org/10.1038/nmeth.4285
Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30 (4): 772–780. https://doi.org/10.1093/molbev/mst010
Mortimer, P.E., Jeewon, R., Xu, J.C., Lumyong, S. & Wanasinghe, D.N. (2021) Morpho-phylo taxonomy of novel Dothideomycetous fungi associated with dead woody twigs in Yunnan Province, China. Frontiers in Microbiology 12: 654683. https://doi.10.3389/fmicb.2021.654683
Nguyen, L.T., Schmidt, H.A., von Haeseler, A. & Minh, B.Q. (2015) IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular biology and evolution 32 (1): 268–274. https://doi.org/10.1093/molbev/ msu300
Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61 (3): 539–542. https://doi.org/10.1093/sysbio/sys029
Shoemaker, R.A., Babcock, C.E. & Irwin, J.A.G. (1991) Massarina walkeri n.sp., the teleomorph of Acrocalymma medicaginis from Medicago sativa contrasted with Leptosphaeria pratensis, L. weimeri n. sp., and L. viridella. Canadian Journal of Botany 69 (3): 569–573. https://doi.org/10.1139/b91-077
Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30 (12): 2725–2729. https://doi.org/10.1093/molbev/mst197
Tennakoon, D.S., Kuo, C.H., Maharachchikumbura, S.S.N., Thambugala, K.M., Gentekaki, E., Phillips, A.L.J., Bhat, D.J., Wanasinghe, D.N., de Silva, N.I., Promputtha, I. & Hyde, K.D. (2021) Taxonomic and phylogenetic contributions to Celtis formosana, Ficus ampelas, F. septica, Macaranga tanarius and Morus australis leaf litter inhabiting microfungi. Fungal Diversity 108: 1–215. https://doi.org/10.1007/s13225-021-00474-w
Trakunyingcharoen, T., Lombard, L., Groenewald, J.Z., Cheewangkoon, R., Toanun, C., Alfenas, A.C. & Crous, P.W. (2014) Mycoparasitic species of Sphaerellopsis, and allied lichenicolous and other genera. IMA Fungus 5: 391–414. https://doi.org/10.5598/imafungus.2014.05.02.05
Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR protocols: a guide to methods and applications, Academic Press, San Diego, California, pp 315–322.
Zhang, H., Hyde, K.D., Mckenzie, E.H., Bahkali, A.H. & Zhou, D. (2012) Sequence data reveals phylogenetic affinities of Acrocalymma aquatica sp. nov., Aquasubmersa mircensis gen. et sp. nov. and Clohesyomyces aquaticus (freshwater coelomycetes). Mycologie 33 (3): 333–346. https://doi.org/10.7872/crym.v33.iss3.2012.333
Zhang, Z.Y., Shao, Q.Y., Li, X., Chen, W.H., Liang, J.D., Han, Y.F., Huang, J.Z. & Liang, Z.Q. (2021) Culturable fungi from urban soils in China I: description of 10 new taxa. Microbiology Spectrum 9: e00867–00821. https://doi.org/10.1128/Spectrum.00867-21