Abstract
The tribe Delphinieae (Ranunculaceae) comprises two species-rich genera, Aconitum and Delphinium, the latter including Consolida and Aconitella. The 650–700 species are distributed in Eurasia and North America; three species occur on tropical African mountains. Many species of Delphinieae are of horticultural importance or medical use and a few are well-studied in terms of genetic diversity and floral morphology and pollination. We sampled 19 populations from 19 Delphinium species throughout Iran. Start Codon Targeted (SCoT) primer were applied to calculate measures of genetic diversity at the population level and genetic differentiation. Amplification of genomic DNA using 10 primers produced 165 bands, of which 150 were polymorphic (94.48%). The obtained high average PIC and MI values revealed high capacity of SCoT primers to detect polymorphic loci among Delphinium. The genetic similarities of 19 collections were estimated from 0.62 to 0.96. According to the SCoT markers analysis, D. lorestanicum and D. oliganthum had the lowest similarity and the species of D. hohenackeri and D. ajacis had the highest similarity.
References
<p>Aitzetmuller, K., Tsevegsuren, N. & Werner, F. (1999) Seed oil fatty acid patterns of Aconitum- Delphinium- Helleborous complex (Ranunculaceae). <em>Plant Systematic and Evolution</em> 213: 37–47. https://doi.org/10.1007/BF00984646</p>
<p>Aarssen LW. (2000) Why are most selfers annuals? A new hypothesis for the fitness benefit of selfing. <em>Oikos</em> 89: 606–612. https://doi.org/10.1034/j.1600-0706.2000.890321.x</p>
<p>Boissier, E.<strong> (</strong>1867) <em>Flora Orientalis</em>, vol. 1. Basel: H. Georg.</p>
<p>Brütting, Christine, Wesche, Karsten; Meyer, Stefan & Hensen, I. (2012) Genetic diversity of six arable plants in relation to their Red List status. <em>Biodiversity and Conservation</em> 21: 745–761. https://doi.org/10.1007/s10531-011-0212-z</p>
<p>Collard, B.C.Y. & Mackill, D.J. (2009) Start codon targeted (SCoT) polymorphism: a simple novel DNA marker technique for generating gene-targeted markers in plants. <em>Plant Molecular Biology Report</em> 27: 86–93. https://doi.org/10.1007/s11105-009-0118-z</p>
<p>Charlesworth, D. (2003) Effects of Inbreeding on the Genetic Diversity of Populations. <em>Philosophical Transactions: Biological Sciences</em> 358 (1434): 1051–1070. [http://www.jstor.org/stable/3558250]</p>
<p>De Candolle, A.P. (1824) <em>Prodromus Systematis Naturalis Regni Vegetabilis.</em> Vol. 2. Paris, Strasbourg, London.</p>
<p>Esfandani-Bozchaloyi, S. & Sheidai, M. (2019) Comparison Of Dna Extraction Methods From <em>Geranium </em>(Geraniaceae). <em>Acta Botanica Hungarica </em>61 (3–4): 251–266. https://doi.org/10.1556/034.61.2019.3-4.3</p>
<p>Esfandani Bozchaloyi, S., Sheidai, M., Keshavarzi, M. & Noormohammadi, Z. (2017a) Genetic diversity and morphological variability in <em>Geranium purpureum </em>Vill. (Geraniaceae) Of Iran. <em>Genetika </em>49: 543–557.</p>
<p>Esfandani Bozchaloyi, S., Sheidai, M., Keshavarzi, M. & Noormohammadi, Z. (2017b) Species delimitation in <em>Geranium </em>Sect. <em>Batrachioidea</em>: Morphological and molecular. <em>Acta Botanica Hungarica </em>59 (3–4): 319–334. https://doi.org/10.1556/034.59.2017.3-4.3</p>
<p>Esfandani Bozchaloyi, S., Sheidai, M., Keshavarzi, M. & Noormohammadi, Z. (2017c) Genetic and morphological diversity in <em>Geranium dissectum </em>(Sec. Dissecta, Geraniaceae) populations. <em>Biologia </em>72 (10): 1121–1130. https://doi.org/10.1515/biolog-2017-0124</p>
<p>Esfandani Bozchaloyi, S., Sheidai, M., Keshavarzi, M. & Noormohammadi, Z. (2017d) Analysis of genetic diversity in <em>Geranium robertianum </em>by ISSR markers. <em>Phytologia Balcanica </em>23 (2): 157–166.</p>
<p>Esfandani Bozchaloyi, S., Sheidai, M., Keshavarzi, M. & Noormohammadi, Z. (2018a) Species Relationship and Population Structure Analysis In <em>Geranium </em>Subg. <em>Robertium </em>(Picard) Rouy with The Use of ISSR Molecular Markers. <em>Acta Botanica Hungarica </em>60 (1–2): 47–65. https://doi.org/10.1556/034.60.2018.1-2.4</p>
<p>Esfandani Bozchaloyi, S., Sheidai, M., Keshavarzi, M. & Noormohammadi, Z. (2018b) Species identification and population structure analysis in <em>Geranium </em>Subg. <em>Geranium </em>(Geraniaceae). <em>Hacquetia </em>17/2: 235–246. https://doi.org/10.1515/hacq-2017-0007</p>
<p>Esfandani Bozchaloyi, S., Sheidai, M., Keshavarzi, M. & Noormohammadi, Z. (2018c) Morphometric and ISSR-analysis of local populations of <em>Geranium molle </em>L. from the southern coast of the Caspian Sea. <em>Cytology and Genetics </em>52 (4): 309–321. https://doi.org/10.3103/S0095452718040102</p>
<p>Evanno, G., Regnaut, S. & Goudet, J. (2005) Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. <em>Molecular Ecology </em>14: 2611–2620. https://doi.org/10.1111/j.1365-294X.2005.02553.x</p>
<p>Freeland, J.R., Kirk, H. & Peterson, S.D. (2011) <em>Molecular Ecology</em> 2<sup>nd</sup> ed. Wiley-Blackwell, UK, 449 pp. https://doi.org/10.1002/9780470979365</p>
<p>Gholamin, R. & Khayatnezhad, M. (2020a) Assessment of the Correlation between Chlorophyll Content and Drought Resistance in Corn Cultivars (<em>Zea Mays</em>). <em>Helix</em> 10 (05): 93–97. https://doi.org/10.29042/2020-10-5-93-97</p>
<p>Gholamin, R. & Khayatnezhad, M. (2020b) The effect of dry season stretch on Chlorophyll Content and RWC of Wheat Genotypes (<em>Triticum Durum</em> L.). <em>Bioscience Biotechnology Research Communications</em> 13 (4): 1833–1829. https://doi.org/10.21786/bbrc/13.4/28</p>
<p>Gholamin, R. & Khayatnezhad, M. (2020c) Study of Bread Wheat Genotype Physiological and Biochemical Responses to Drought Stress. <em>Helix</em> 10 (05): 87–92. https://doi.org/10.29042/2020-10-5-87-92</p>
<p>Guo, L.-N., She, C., Kong, D.-B., Yan, S.-L., Xu, P., Khayatnezhad, M. & Gholinia, F. (2021) Prediction of the effects of climate change on hydroelectric generation, electricity demand, and emissions of greenhouse gases under climatic scenarios and optimized ANN model. <em>Energy Reports</em> 7: 5431–5445. https://doi.org/10.1016/j.egyr.2021.08.134</p>
<p>Gray, S.F. (1821) A <em>natural arrangement of British plants</em>. vol. 2. London.</p>
<p>Hong, De-Y. (1986) Biosystematic observation on 5 species of <em>Consolida </em>(Ranunculaceae). <em>Acta Botanica Sinica</em> 28: 1–10.</p>
<p>Hou, R., Li, S., Wu, M., Ren, G., Gao, W., Khayatnezhad, M. & Gholinia, F. (2021) Assessing of impact climate parameters on the gap between hydropower supply and electricity demand by RCPs scenarios and optimized ANN by the improved Pathfinder (IPF) algorithm. <em>Energy</em> 237: 601–621. https://doi.org/10.1016/j.energy.2021.121621</p>
<p>Huang, D., Wang, J. & Khayatnezhad, M. (2021) Estimation of Actual Evapotranspiration Using Soil Moisture Balance and Remote Sensing. <em>Iranian Journal of Science and Technology, Transactions of Civil Engineering </em>45: 2779–2786. https://doi.org/10.1007/s40996-020-00575-7</p>
<p>Hammer, Ø., Harper, Dat. & Ryan, P.D. (2001) PAST: Paleontological Statistics software package for education and data analysis<em>. Palaeontologia Electronica</em> 4: 1−9.</p>
<p>Iranshahr, M., Rechinger f., K.H. & Riedl, H. (1992) <em>Delphinium </em>L. (Ranunculaceae).<em> In: </em>Rechinger, K.H. (Ed.) <em>Flora Iranica.</em> no. 171: Akademische Druckund-Verlagsanstalt, Graz, pp. 89–114.</p>
<p>Jia, Y., Khayatnezhad, M. & Mehri, S. (2020) Population differentiation and gene flow in Rrodium cicutarium: A potential medicinal plant. <em>Genetika </em>52 (3): 1127–1144. https://doi.org/10.2298/GENSR2003127J</p>
<p>Jabbour, F. & Renner, S.S. (2011) Consolida and Aconitella are an annual clade of Delphinium (Ranunculaceae) that diversified in the Mediterranean basin and the Irano-Turanian region. <em>Taxon</em> 60: 1029–1040. https://doi.org/10.1002/tax.604007</p>
<p>Jabbour, F. & Renner, S. (2012) A phylogeny of Delphinieae (Ranunculaceae) shows that <em>Aconitum</em> is nested within Delphinium and that Late Miocene transitions to long life cycles in the Himalayas and Southwest China coincide with bursts in diversification. <em>Molecular Phylogenetic Evolution </em>62: 928–942. https://doi.org/10.1016/j.ympev.2011.12.005</p>
<p>Karasakal, A., Khayatnezhad, M. & Gholamin, R. (2020a) The Durum Wheat Gene Sequence Response Assessment of Triticum durum for Dehydration Situations Utilizing Different Indicators of Water Deficiency. <em>Bioscience Biotechnology Research Communications</em> 13 (4): 2050–2057. https://doi.org/10.21786/bbrc/13.4/62</p>
<p>Karasakal, A., Khayatnezhad, M. & Gholamin, R. (2020b) The Effect of Saline, Drought, and Presowing Salt Stress on Nitrate Reductase Activity in Varieties of Eleusine coracana (Gaertn). <em>Bioscience Biotechnology Research Communications</em> 13 (4): 2087–2091. https://doi.org/10.21786/bbrc/13.4/68</p>
<p>Khayatnezhad, M. & Gholamin, R. (2020) Study of Durum Wheat Genotypes’ Response to Drought Stress Conditions. <em>Helix</em> 10 (05): 98–103. https://doi.org/10.29042/2020-10-5-98-103</p>
<p>Khayatnezhad, M. & Gholamin, R. (2021a) The Effect of Drought Stress on the Superoxide Dismutase and Chlorophyll Content in Durum Wheat Genotypes<em>. Advancements in Life Sciences </em>8 (2): 119–123.</p>
<p>Khayatnezhad, M. & Gholamin, R. (2021b) Impacts of Drought Stress on Corn Cultivars (Zea mays L.) At the Germination Stage. <em>Bioscience Research</em> 18 (1): 409–414.</p>
<p>Leimu, R., Vergeer, P., Angeloni, F. & Ouborg, N.J. (2010) Habitat fragmentation, climate change and inbreeding in plants. <em>Annals</em> of the <em>New York Academy</em> of <em>Sciences</em> 1195: 84–98. https://doi.org/10.1111/j.1749-6632.2010.05450.x</p>
<p>Ma, S., Khayatnezhad, M. & Minaeifar, A.A. (2021b) Genetic diversity and relationships among Hypericum L. species by ISSR Markers: A high value medicinal plant from Northern of Iran. <em>Caryologia</em> 74 (1): 97–107. https://doi.org/10.36253/caryologia-968</p>
<p>Nybom, H. (2004) Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants. <em>Molecular and Ecology</em> 13: 1143–1155. https://doi.org/10.1111/j.1365-294X.2004.02141.x</p>
<p>Orellana, M.R., Blanché, C., Simon, J. & Bosch, M. (2009) Genetic diversity within and among disjunct populations of the Mediterranean Island endemic Delphinium pictum and D. requienii (Ranunculaceae). <em>Folia Geobotanic</em> 44: 47–63. https://doi.org/10.1007/s12224-009-9028-y</p>
<p>Potts, G.R., Ewald, J.A. & Aebischer, N.J. (2010a) Long-term changes in the flora of the cereal ecosystem on the Sussex Downs, England, focusing on the years 1968–2005. <em>Journal of Applied Ecology</em> 47: 215–226. https://doi.org/10.1111/j.1365-2664.2009.01742.x</p>
<p>Peakall, R. & Smouse, P.E. (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. <em>Molecular Ecology Notes</em> 6: 288–295. https://doi.org/10.1111/j.1471-8286.2005.01155.x</p>
<p>Peng, X., Khayatnezhad, M. & Ghezeljehmeidan, L. (2021) Rapd profiling in detecting genetic variation in stellaria l. (caryophyllaceae).<em> Genetika</em> 53 (1): 349–362. https://doi.org/10.2298/GENSR2101349P</p>
<p>Podani, J. (2000) <em>Introduction to the Exploration of Multivariate Data English translation. </em>Backhuyes Publisher, Leide, 407 pp.</p>
<p>Ren, J. & Khayatnezhad, M. (2021) Evaluating the stormwater management model to improve urban water allocation system in drought conditions. <em>Water Supply </em>21 (4): 1514–1524. https://doi.org/10.2166/ws.2021.027</p>
<p>Si, X., Gao, L. Song, Y. Khayatnezhad, M. & Minaeifar, A.A. (2020) Understanding population differentiation using geographical, morphological and genetic characterization in Erodium cicunium. <em>Indian Journal of Genetic </em>80 (4): 459–467. https://doi.org/10.31742/IJGPB.80.4.12</p>
<p>Sun, Q., Lin, D., Khayatnezhad, M. & Taghavi, M. (2021) Investigation of phosphoric acid fuel cell, linear Fresnel solar reflector and Organic Rankine Cycle polygeneration energy system in different climatic conditions. <em>Process Safety and Environmental Protection</em> 147: 993–1008. https://doi.org/10.1016/j.psep.2021.01.035</p>
<p>Sun, X. & Khayatnezhad, M. (2021) Fuzzy-probabilistic modeling the flood characteristics using bivariate frequency analysis and α-cut decomposition. <em>Water Supply</em> 21 (8): 4391–4403. https://doi.org/10.2166/ws.2021.186</p>
<p>Svensson, R. & Wigren, M. (1986) History and biology of Consolida regalis in Sweden. <em>Svensk Botanisk Tidskrift</em> 80: 31–53.</p>
<p>Sivaprakash, K.R., Prasanth, S.R., Mohanty, B.P. & Parida, A. (2004) Genetic diversity of black gram landraces as evaluated by AFLP markers. <em>Current Science </em>86: 1411–1415.</p>
<p>Turabekova, M.A., Rasulev, B.F., Dzhakhangirov, F.N., Leszczynska, D., Leszczynski, J., (2010) Aconitum and Delphinium alkaloids of curare-like activity. QSAR analysis and molecular docking of alkaloids into AChBP. <em>European Journal of Medicinal Chemistry</em> 45: 3885–3894. https://doi.org/10.1016/j.ejmech.2010.05.042</p>
<p>Tamura, M.<strong> (</strong>1966) Morphology, ecology and phylogeny of the Ranunculaceae VI. <em>Science Reports</em> South <em>College</em>, <em>North</em> <em>College</em>, <em>Osaka University</em> 15: 13–35.</p>
<p>Trifonova, VI.<strong> (</strong>1990) Comparative biomorphological study of the taxonomy and phylogeny of the genera <em>Consolida </em>(DC.) S.F. Gray and <em>Aconitella </em>Spach. <em>Collect Bot (Barcelona) </em>19: 97–110. https://doi.org/10.3989/collectbot.1990.v19.119</p>
<p>Tams, S.H., Melchinger, A.E. & Bauer, E. (2005) Genetic similarity among European winter triticale elite germplasms assessed with AFLP and comparisons with SSR and pedigree data. <em>Plant Breeding </em>124: 154–160. https://doi.org/10.1111/j.1439-0523.2004.01047.x</p>
<p>Wang, C., Shang, Y. & Khayatnezhad, M. (2021) Fuzzy Stress-based Modeling for Probabilistic Irrigation Planning Using Copula-NSPSO. <em>Water Resources Management </em>35 (14): 4943–4959.<em> h</em>ttps://doi.org/10.1007/s11269-021-02981-6</p>
<p>Yin, J., Khayatnezhad, M. & Shakoor, A. (2021) Evaluation Of Genetic Diversity In Geranium (Geraniaceae) Using Rapd Marker. <em>Genetika</em> 53 (1): 363–378. https://doi.org/10.2298/GENSR2101363Y</p>
<p>Zheng, R., Zhao, S. Khayyatnezhad, M. & Afzal Shah, S. (2021) Comparative study and genetic diversity in Salvia (Lamiaceae) using RAPD Molecular Markers. <em>Caryologia</em> 74 (2): 45–56. https://doi.org/10.36253/caryologia-1236</p>
<p>Zhu, K., Liu, L. Li, S. Li, B., Khayatnezhad, M. & Shakoor, A. (2021) Morphological method and molecular marker determine genetic diversity and population structure in Allochrusa. <em>Caryologia</em> 74 (2): 121–130. https://doi.org/10.36253/caryologia-958</p>
<p>Aarssen LW. (2000) Why are most selfers annuals? A new hypothesis for the fitness benefit of selfing. <em>Oikos</em> 89: 606–612. https://doi.org/10.1034/j.1600-0706.2000.890321.x</p>
<p>Boissier, E.<strong> (</strong>1867) <em>Flora Orientalis</em>, vol. 1. Basel: H. Georg.</p>
<p>Brütting, Christine, Wesche, Karsten; Meyer, Stefan & Hensen, I. (2012) Genetic diversity of six arable plants in relation to their Red List status. <em>Biodiversity and Conservation</em> 21: 745–761. https://doi.org/10.1007/s10531-011-0212-z</p>
<p>Collard, B.C.Y. & Mackill, D.J. (2009) Start codon targeted (SCoT) polymorphism: a simple novel DNA marker technique for generating gene-targeted markers in plants. <em>Plant Molecular Biology Report</em> 27: 86–93. https://doi.org/10.1007/s11105-009-0118-z</p>
<p>Charlesworth, D. (2003) Effects of Inbreeding on the Genetic Diversity of Populations. <em>Philosophical Transactions: Biological Sciences</em> 358 (1434): 1051–1070. [http://www.jstor.org/stable/3558250]</p>
<p>De Candolle, A.P. (1824) <em>Prodromus Systematis Naturalis Regni Vegetabilis.</em> Vol. 2. Paris, Strasbourg, London.</p>
<p>Esfandani-Bozchaloyi, S. & Sheidai, M. (2019) Comparison Of Dna Extraction Methods From <em>Geranium </em>(Geraniaceae). <em>Acta Botanica Hungarica </em>61 (3–4): 251–266. https://doi.org/10.1556/034.61.2019.3-4.3</p>
<p>Esfandani Bozchaloyi, S., Sheidai, M., Keshavarzi, M. & Noormohammadi, Z. (2017a) Genetic diversity and morphological variability in <em>Geranium purpureum </em>Vill. (Geraniaceae) Of Iran. <em>Genetika </em>49: 543–557.</p>
<p>Esfandani Bozchaloyi, S., Sheidai, M., Keshavarzi, M. & Noormohammadi, Z. (2017b) Species delimitation in <em>Geranium </em>Sect. <em>Batrachioidea</em>: Morphological and molecular. <em>Acta Botanica Hungarica </em>59 (3–4): 319–334. https://doi.org/10.1556/034.59.2017.3-4.3</p>
<p>Esfandani Bozchaloyi, S., Sheidai, M., Keshavarzi, M. & Noormohammadi, Z. (2017c) Genetic and morphological diversity in <em>Geranium dissectum </em>(Sec. Dissecta, Geraniaceae) populations. <em>Biologia </em>72 (10): 1121–1130. https://doi.org/10.1515/biolog-2017-0124</p>
<p>Esfandani Bozchaloyi, S., Sheidai, M., Keshavarzi, M. & Noormohammadi, Z. (2017d) Analysis of genetic diversity in <em>Geranium robertianum </em>by ISSR markers. <em>Phytologia Balcanica </em>23 (2): 157–166.</p>
<p>Esfandani Bozchaloyi, S., Sheidai, M., Keshavarzi, M. & Noormohammadi, Z. (2018a) Species Relationship and Population Structure Analysis In <em>Geranium </em>Subg. <em>Robertium </em>(Picard) Rouy with The Use of ISSR Molecular Markers. <em>Acta Botanica Hungarica </em>60 (1–2): 47–65. https://doi.org/10.1556/034.60.2018.1-2.4</p>
<p>Esfandani Bozchaloyi, S., Sheidai, M., Keshavarzi, M. & Noormohammadi, Z. (2018b) Species identification and population structure analysis in <em>Geranium </em>Subg. <em>Geranium </em>(Geraniaceae). <em>Hacquetia </em>17/2: 235–246. https://doi.org/10.1515/hacq-2017-0007</p>
<p>Esfandani Bozchaloyi, S., Sheidai, M., Keshavarzi, M. & Noormohammadi, Z. (2018c) Morphometric and ISSR-analysis of local populations of <em>Geranium molle </em>L. from the southern coast of the Caspian Sea. <em>Cytology and Genetics </em>52 (4): 309–321. https://doi.org/10.3103/S0095452718040102</p>
<p>Evanno, G., Regnaut, S. & Goudet, J. (2005) Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. <em>Molecular Ecology </em>14: 2611–2620. https://doi.org/10.1111/j.1365-294X.2005.02553.x</p>
<p>Freeland, J.R., Kirk, H. & Peterson, S.D. (2011) <em>Molecular Ecology</em> 2<sup>nd</sup> ed. Wiley-Blackwell, UK, 449 pp. https://doi.org/10.1002/9780470979365</p>
<p>Gholamin, R. & Khayatnezhad, M. (2020a) Assessment of the Correlation between Chlorophyll Content and Drought Resistance in Corn Cultivars (<em>Zea Mays</em>). <em>Helix</em> 10 (05): 93–97. https://doi.org/10.29042/2020-10-5-93-97</p>
<p>Gholamin, R. & Khayatnezhad, M. (2020b) The effect of dry season stretch on Chlorophyll Content and RWC of Wheat Genotypes (<em>Triticum Durum</em> L.). <em>Bioscience Biotechnology Research Communications</em> 13 (4): 1833–1829. https://doi.org/10.21786/bbrc/13.4/28</p>
<p>Gholamin, R. & Khayatnezhad, M. (2020c) Study of Bread Wheat Genotype Physiological and Biochemical Responses to Drought Stress. <em>Helix</em> 10 (05): 87–92. https://doi.org/10.29042/2020-10-5-87-92</p>
<p>Guo, L.-N., She, C., Kong, D.-B., Yan, S.-L., Xu, P., Khayatnezhad, M. & Gholinia, F. (2021) Prediction of the effects of climate change on hydroelectric generation, electricity demand, and emissions of greenhouse gases under climatic scenarios and optimized ANN model. <em>Energy Reports</em> 7: 5431–5445. https://doi.org/10.1016/j.egyr.2021.08.134</p>
<p>Gray, S.F. (1821) A <em>natural arrangement of British plants</em>. vol. 2. London.</p>
<p>Hong, De-Y. (1986) Biosystematic observation on 5 species of <em>Consolida </em>(Ranunculaceae). <em>Acta Botanica Sinica</em> 28: 1–10.</p>
<p>Hou, R., Li, S., Wu, M., Ren, G., Gao, W., Khayatnezhad, M. & Gholinia, F. (2021) Assessing of impact climate parameters on the gap between hydropower supply and electricity demand by RCPs scenarios and optimized ANN by the improved Pathfinder (IPF) algorithm. <em>Energy</em> 237: 601–621. https://doi.org/10.1016/j.energy.2021.121621</p>
<p>Huang, D., Wang, J. & Khayatnezhad, M. (2021) Estimation of Actual Evapotranspiration Using Soil Moisture Balance and Remote Sensing. <em>Iranian Journal of Science and Technology, Transactions of Civil Engineering </em>45: 2779–2786. https://doi.org/10.1007/s40996-020-00575-7</p>
<p>Hammer, Ø., Harper, Dat. & Ryan, P.D. (2001) PAST: Paleontological Statistics software package for education and data analysis<em>. Palaeontologia Electronica</em> 4: 1−9.</p>
<p>Iranshahr, M., Rechinger f., K.H. & Riedl, H. (1992) <em>Delphinium </em>L. (Ranunculaceae).<em> In: </em>Rechinger, K.H. (Ed.) <em>Flora Iranica.</em> no. 171: Akademische Druckund-Verlagsanstalt, Graz, pp. 89–114.</p>
<p>Jia, Y., Khayatnezhad, M. & Mehri, S. (2020) Population differentiation and gene flow in Rrodium cicutarium: A potential medicinal plant. <em>Genetika </em>52 (3): 1127–1144. https://doi.org/10.2298/GENSR2003127J</p>
<p>Jabbour, F. & Renner, S.S. (2011) Consolida and Aconitella are an annual clade of Delphinium (Ranunculaceae) that diversified in the Mediterranean basin and the Irano-Turanian region. <em>Taxon</em> 60: 1029–1040. https://doi.org/10.1002/tax.604007</p>
<p>Jabbour, F. & Renner, S. (2012) A phylogeny of Delphinieae (Ranunculaceae) shows that <em>Aconitum</em> is nested within Delphinium and that Late Miocene transitions to long life cycles in the Himalayas and Southwest China coincide with bursts in diversification. <em>Molecular Phylogenetic Evolution </em>62: 928–942. https://doi.org/10.1016/j.ympev.2011.12.005</p>
<p>Karasakal, A., Khayatnezhad, M. & Gholamin, R. (2020a) The Durum Wheat Gene Sequence Response Assessment of Triticum durum for Dehydration Situations Utilizing Different Indicators of Water Deficiency. <em>Bioscience Biotechnology Research Communications</em> 13 (4): 2050–2057. https://doi.org/10.21786/bbrc/13.4/62</p>
<p>Karasakal, A., Khayatnezhad, M. & Gholamin, R. (2020b) The Effect of Saline, Drought, and Presowing Salt Stress on Nitrate Reductase Activity in Varieties of Eleusine coracana (Gaertn). <em>Bioscience Biotechnology Research Communications</em> 13 (4): 2087–2091. https://doi.org/10.21786/bbrc/13.4/68</p>
<p>Khayatnezhad, M. & Gholamin, R. (2020) Study of Durum Wheat Genotypes’ Response to Drought Stress Conditions. <em>Helix</em> 10 (05): 98–103. https://doi.org/10.29042/2020-10-5-98-103</p>
<p>Khayatnezhad, M. & Gholamin, R. (2021a) The Effect of Drought Stress on the Superoxide Dismutase and Chlorophyll Content in Durum Wheat Genotypes<em>. Advancements in Life Sciences </em>8 (2): 119–123.</p>
<p>Khayatnezhad, M. & Gholamin, R. (2021b) Impacts of Drought Stress on Corn Cultivars (Zea mays L.) At the Germination Stage. <em>Bioscience Research</em> 18 (1): 409–414.</p>
<p>Leimu, R., Vergeer, P., Angeloni, F. & Ouborg, N.J. (2010) Habitat fragmentation, climate change and inbreeding in plants. <em>Annals</em> of the <em>New York Academy</em> of <em>Sciences</em> 1195: 84–98. https://doi.org/10.1111/j.1749-6632.2010.05450.x</p>
<p>Ma, S., Khayatnezhad, M. & Minaeifar, A.A. (2021b) Genetic diversity and relationships among Hypericum L. species by ISSR Markers: A high value medicinal plant from Northern of Iran. <em>Caryologia</em> 74 (1): 97–107. https://doi.org/10.36253/caryologia-968</p>
<p>Nybom, H. (2004) Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants. <em>Molecular and Ecology</em> 13: 1143–1155. https://doi.org/10.1111/j.1365-294X.2004.02141.x</p>
<p>Orellana, M.R., Blanché, C., Simon, J. & Bosch, M. (2009) Genetic diversity within and among disjunct populations of the Mediterranean Island endemic Delphinium pictum and D. requienii (Ranunculaceae). <em>Folia Geobotanic</em> 44: 47–63. https://doi.org/10.1007/s12224-009-9028-y</p>
<p>Potts, G.R., Ewald, J.A. & Aebischer, N.J. (2010a) Long-term changes in the flora of the cereal ecosystem on the Sussex Downs, England, focusing on the years 1968–2005. <em>Journal of Applied Ecology</em> 47: 215–226. https://doi.org/10.1111/j.1365-2664.2009.01742.x</p>
<p>Peakall, R. & Smouse, P.E. (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. <em>Molecular Ecology Notes</em> 6: 288–295. https://doi.org/10.1111/j.1471-8286.2005.01155.x</p>
<p>Peng, X., Khayatnezhad, M. & Ghezeljehmeidan, L. (2021) Rapd profiling in detecting genetic variation in stellaria l. (caryophyllaceae).<em> Genetika</em> 53 (1): 349–362. https://doi.org/10.2298/GENSR2101349P</p>
<p>Podani, J. (2000) <em>Introduction to the Exploration of Multivariate Data English translation. </em>Backhuyes Publisher, Leide, 407 pp.</p>
<p>Ren, J. & Khayatnezhad, M. (2021) Evaluating the stormwater management model to improve urban water allocation system in drought conditions. <em>Water Supply </em>21 (4): 1514–1524. https://doi.org/10.2166/ws.2021.027</p>
<p>Si, X., Gao, L. Song, Y. Khayatnezhad, M. & Minaeifar, A.A. (2020) Understanding population differentiation using geographical, morphological and genetic characterization in Erodium cicunium. <em>Indian Journal of Genetic </em>80 (4): 459–467. https://doi.org/10.31742/IJGPB.80.4.12</p>
<p>Sun, Q., Lin, D., Khayatnezhad, M. & Taghavi, M. (2021) Investigation of phosphoric acid fuel cell, linear Fresnel solar reflector and Organic Rankine Cycle polygeneration energy system in different climatic conditions. <em>Process Safety and Environmental Protection</em> 147: 993–1008. https://doi.org/10.1016/j.psep.2021.01.035</p>
<p>Sun, X. & Khayatnezhad, M. (2021) Fuzzy-probabilistic modeling the flood characteristics using bivariate frequency analysis and α-cut decomposition. <em>Water Supply</em> 21 (8): 4391–4403. https://doi.org/10.2166/ws.2021.186</p>
<p>Svensson, R. & Wigren, M. (1986) History and biology of Consolida regalis in Sweden. <em>Svensk Botanisk Tidskrift</em> 80: 31–53.</p>
<p>Sivaprakash, K.R., Prasanth, S.R., Mohanty, B.P. & Parida, A. (2004) Genetic diversity of black gram landraces as evaluated by AFLP markers. <em>Current Science </em>86: 1411–1415.</p>
<p>Turabekova, M.A., Rasulev, B.F., Dzhakhangirov, F.N., Leszczynska, D., Leszczynski, J., (2010) Aconitum and Delphinium alkaloids of curare-like activity. QSAR analysis and molecular docking of alkaloids into AChBP. <em>European Journal of Medicinal Chemistry</em> 45: 3885–3894. https://doi.org/10.1016/j.ejmech.2010.05.042</p>
<p>Tamura, M.<strong> (</strong>1966) Morphology, ecology and phylogeny of the Ranunculaceae VI. <em>Science Reports</em> South <em>College</em>, <em>North</em> <em>College</em>, <em>Osaka University</em> 15: 13–35.</p>
<p>Trifonova, VI.<strong> (</strong>1990) Comparative biomorphological study of the taxonomy and phylogeny of the genera <em>Consolida </em>(DC.) S.F. Gray and <em>Aconitella </em>Spach. <em>Collect Bot (Barcelona) </em>19: 97–110. https://doi.org/10.3989/collectbot.1990.v19.119</p>
<p>Tams, S.H., Melchinger, A.E. & Bauer, E. (2005) Genetic similarity among European winter triticale elite germplasms assessed with AFLP and comparisons with SSR and pedigree data. <em>Plant Breeding </em>124: 154–160. https://doi.org/10.1111/j.1439-0523.2004.01047.x</p>
<p>Wang, C., Shang, Y. & Khayatnezhad, M. (2021) Fuzzy Stress-based Modeling for Probabilistic Irrigation Planning Using Copula-NSPSO. <em>Water Resources Management </em>35 (14): 4943–4959.<em> h</em>ttps://doi.org/10.1007/s11269-021-02981-6</p>
<p>Yin, J., Khayatnezhad, M. & Shakoor, A. (2021) Evaluation Of Genetic Diversity In Geranium (Geraniaceae) Using Rapd Marker. <em>Genetika</em> 53 (1): 363–378. https://doi.org/10.2298/GENSR2101363Y</p>
<p>Zheng, R., Zhao, S. Khayyatnezhad, M. & Afzal Shah, S. (2021) Comparative study and genetic diversity in Salvia (Lamiaceae) using RAPD Molecular Markers. <em>Caryologia</em> 74 (2): 45–56. https://doi.org/10.36253/caryologia-1236</p>
<p>Zhu, K., Liu, L. Li, S. Li, B., Khayatnezhad, M. & Shakoor, A. (2021) Morphological method and molecular marker determine genetic diversity and population structure in Allochrusa. <em>Caryologia</em> 74 (2): 121–130. https://doi.org/10.36253/caryologia-958</p>