Abstract
Four species of Xylaria were collected from Guizhou and Hainan provinces in China. Morphological and phylogenetic analyses (based on β-tubulin, rpb2 and α-actin genes) revealed two new species (X. sylvatica sp. nov. and X. wuzhishanensis sp. nov.) and two known species (X. atrosphaerica and X. cubensis). Xylaria sylvatica is distinguished from X. spinulosa by lacking long thorns on the stromatal surface, and larger ascospores (23–28.5 × 7–9.5 μm vs. 19–23 × 6.5–8.5 μm) with a sigmoid germ slit. Xylaria wuzhishanensis differs from X. schweinitzii by its semi-globular or irregularly globose stromata, from X. atrosphaerica by its larger ascospores (23–30.5 × 7–9 μm vs. 17–25 × 6–10 μm). Their descriptions and illustrations are provided.
References
Callan, B.E. & Rogers, J.D. (1990) Teleomorph-anamorph connections and correlations in some Xylaria species. Mycotaxon 36: 343–369.
Cheng, M.J., Wu, M.D., Su, Y.S., Chan, H.Y., Hsieh, S.Y., Chen, Y.L., Chen, J.J., Chou, Y.T., Hsiao, Y.C. & Wu, H.S. (2019) Additional chemical constituents of an endophytic fungus Xylaria papulis. Chemistry of Natural Compounds 55: 340–342. http://doi.org/10.1007/s10600-019-02684-w
Cruz, K.S. & Cortez, V.G. (2015) Xylaria (Xylariaceae, Ascomycota) in the Parque Estadual de So Camilo, Paraná, Brazil. Acta Biológica Paranaense 44 (3–4): 129–144. http://doi.org/10.5380/abpr.v44i1-4.43846
Cui, B.K., Li, H.J., Ji, X., Zhou, J.L., Song, J., Si, J., Yang, Z.L. & Dai, Y.C. (2019) Species diversity, taxonomy and phylogeny of Polyporaceae (Basidiomycota) in China. Fungal Diversity 97: 137–392. http://doi.org/10.1007/s13225-019-00427-4
Dai, Y.C. (2012) Polypore diversity in China with an annotated checklist of Chinese polypores. Mycoscience 53: 49–80. http://doi.org/10.1007/s10267-011-0134-3
Dai, Y.C., Yang, Z.L., Cui, B.K., Yu, C.J. & Zhou, L.W. (2009) Species diversity and utilization of medicinal mushrooms and fungi in China. International Journal of Medicinal Mushrooms 11: 287–302. http://doi.org/10.1615/IntJMedMushr.v11.i3.80
Daranagama, D.A., Hyde, K.D., Sir, E.B., Thambugala, K.M., Tian, Q., Samarakoon, M.C., McKenzie, E.H.C., Jayasiri, S.C., Tibpromma, S., Bhat, J.D., Liu, X.Z. & Stadler, M. (2018) Towards a natural classification and backbone tree for Graphostromataceae, Hypoxylaceae, Lopadostomataceae and Xylariaceae. Fungal Diversity 88: 1–165. http://doi.org/10.1007/s13225-017-0388-y
Fournier, J., Flessa, F., Peršoh, D. & Stadler, M. (2011) Three new Xylaria species from southwestern Europe. Mycological Progress 10: 33–52. http://doi.org/10.1007/s11557-010-0671-8
Fournier, J., Lechat, C. & Courtecuisse, R. (2018) The genus Xylaria sensu lato (Xylariaceae) in Guadeloupe and Martinique (French West Indies) I. Taxa with penzigioid stromata. Ascomycete.org 10 (4): 131–176. http://doi.org/10.25664/ART-0239
Fournier, J., Lechat, C. & Courtecuisse, R. (2020) The genus Xylaria sensu lato (Xylariaceae) in Guadeloupe and Martinique (French West Indies) III. Taxa with slender upright stromata. Ascomycete.org 12 (3): 81–164. http://doi.org/10.25664/ART-0302
Gao, Q. & Yang, Z.L. (2016) Diversity and distribution patterns of root-associated fungi on herbaceous plants in alpine meadows of southwestern China. Mycologia 108: 281–291. http://doi.org/10.3852/14-324
Greville, R.K. (1824) Flora Edinensis: Or A Description of Plants Growing Near Edinburgh with a Concise Introduction to the Natural Orders of the Class Cryptogamia: Blackwood.
Hall, T.A. (2006) Bioedit. Department of Microbiology, North Carolina State University. [http://wwwmbioncsuedu/Bioedit/Bioedithtml]
Han, J. & Ma, J. (2005) Molecular and morphological investigation of the genus Daldinia in Northern Europe. Trace Elements Science 104: 275–280. https://doi.org/10.1017/S0953756299001719
Hande, D.V. & Hiwarale, S.V. (2013) Diversity of Xylaria Species from Amravati Region, Amravati, MS, India. International Research Journal of Biological Sciences 2 (1): 67–69.
Hashemi, S.A., Zare, R., Khodaparast, S.A. & Elahinia, S.A. (2015) A new Xylaria species from Iran. Mycologica Iranica 2 (1): 1–10. https://doi.org/10.22043/MI.2015.13603
Hashemi, S.A., Khodaparast, S.A., Zare, R. & Elahinia, S.A. (2014) Contribution to the identification of Xylaria species in Iran. Rostaniha 15 (2): 153–166. https://doi.org/10.22092/BOTANY.2014.101239
Himani, S. & Krishnappa, M. (2020) Xylaria oxyacanthae (Xylariaceae), a new record on Diospyros melanoxylon from India. Studies in Fungi 5 (1): 485–490. https://doi.org/10.5943/sif/5/1/29
Hsieh, H.M., Ju, Y.M. & Rogers, J.D. (2005) Molecular phylogeny of Hypoxylon and related genera. Mycologia 97 (4): 844–865. https://doi.org/10.3852/mycologia.97.4.844
Hsien, H.M., Lin, C.R., Fang, M.J., Rogers, J.D., Fourier, J., Lechat, C. & Ju, Y.M. (2010) Phylogenetic status of Xylaria subgenus Pseudoxylaria among taxa of the subfamily Xylarioideae (Xylariaceae) and phylogeny of the taxa involved in the subfamily. Molecular Phylogenetics and Evolution 54: 957–969. http://doi.org/10.1016/j.ympev.2009.12.015
Huang, G., Guo, L. & Liu, N. (2014) Two new species of Xylaria and X. diminuta new to China. Mycotaxon 129 (1): 149–152. http://doi.org/10.5248/129.149
Huang, G., Guo, L. & Liu, N. (2014) Xylaria byttneriae sp.nov.from yunnan province in China. Mycosystema 33 (3): 567–570. http://doi.org/10.13346/j.mycosystema.130259
Huang, G., Wang, R., Guo, L. & Liu, N. (2015) Three new species of Xylaria from China. Mycotaxon 130 (1): 299–304. http://doi.org/10.5248/130.299
Huang, Y., Yuan, X.H., He, X.S. & Song, H. (2016) Analysis of hplc fingerprint of Xylaria striata by different culture methods. Journal of Anhui Agricultural Sciences 44 (08): 134–137. http://doi.org/10.13989/j.cnki.0517-6611.2016.08.048
Husbands, D.R., Urbina, H., Lewis, S.M. & Aime, M.C. (2018) Xylaria karyophthora: a new seed-inhabiting fungus of Green heart from Guyana. Mycologia 110: 434–447. http://doi.org/10.1080/00275514.2018.1457349
Ibrahim, A., Tanney, J.B., Fei, F., Seifert, K.A., Cutler, G.C., Capretta, A., Mille, J.D. & Sumarah, M.K. (2020) Metabolomic-guided discovery of cyclic nonribosomal peptides from Xylaria ellisii sp. nov. a leaf and stem endophyte of Vaccinium angustifolium. Scientific Reports 10: 4599 https://doi.org/10.1038/s41598-020-61088-x
Johnston, P.R., Rogers, J.D., Park, D. & Martin, N.A. (2016) Entalbostroma erumpens gen. et sp. nov. (Xylariaceae) from Phormium in New Zealand. Mycotaxon 131: 765–771. http://doi.org/10.5248/131.765
Ju, Y.M. & Hsieh, H.M. (2007) Xylaria species associated with nests of Odontotermes formosanus in Taiwan. Mycologia 99: 936–957. http://doi.org/10.1080/15572536.2007.11832525
Ju, Y.M., Hsieh, H.M., Rogers, J.D., Fournier, J., Jaklitsch, W.M. & Courtecuisse, R. (2012) New and interesting penzigioid Xylaria species with small, soft stromata. Mycologia 104 (3): 766–776. http://doi.org/10.3852/11-313
Ju, Y.M., Hsieh, H.M., Vasilyeva, L. & Akulov, A. (2009) Three new Xylaria species from Russian Far East. Mycologia 101: 548–553. http://doi.org/10.3852/08-188
Ju, Y.M. & Rogers, J.D. (1999) The Xylariaceae of Taiwan (excluding Anthostomella). Mycotaxon 73: 343–440.
Ju, Y.M., Rogers, J.D. & Hsieh, H.M. (2018) Xylaria species associated with fallen fruits and seeds. Mycologia 110 (4): 726–749. http://doi.org/10.1080/00275514.2018.1469879
Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30: 772–780. http://doi.org/10.1093/molbev/mst010
Karun, N.C. & Sridhar, K.R. (2015) Xylaria complex in the South Western India. Plant Pathology & Quarantine 5 (2): 83–96. http://doi.org/10.5943/ppq/5/2/7
Kirk, P.M., Cannon, P.F., Minter, D.W. & Stalpers, J.A. (2008) Dictionary of the Fungi. Commonwealth Mycological Institute.
Konta, S., Hyde, K.D., Phookamsak, R., Xu, J.C. & Lu, Y.Z. (2020) Polyphyletic genera in Xylariaceae (Xylariales): Neoxylaria gen. nov. and Stilbohypoxylon. Mycosphere 11 (1): 2629–2651. http://doi.org/10.5943/mycosphere/11/1/17
Koyani, R.D., Patel, H.R., Vasava, A.M. & Rajput, K.S. (2016) Xylariaceae: Overview and addition to fungal diversity of Gujarat state. Studies in Fungi 1 (1): 69–79. http://doi.org/10.5943/sif/1/1/6
Lee, Y.S., Han, S.S. & Jeong, I.S. (2002) Taxonomical Characteristics of Xylaria spp. Collected from Malaysia. Mycobiology 30 (4): 193–196. https://doi.org/10.4489/MYCO.2002.30.4.193
Lee, J.S., Ko, K.S. & J, H.S. (2000) Phylogenetic analysis of Xylaria based on nuclear ribosomal ITS1-5.8S-ITS2 sequences. Fems Microbiology Letters 1: 89–93. http://doi.org/10.1016/S0378-1097(00)00181-6
Li, Q.R., Kang, J.C. & Hyde, K.D. (2015) Two new species of the genus Collodiscula (Xylariaceae) from China. Mycological Progress 14: 52. http://doi.org/10.1007/s11557-015-1075-6
Li, Q.R., Liu, L.L., Zhang, X., Shen, X.C. & Kang, J.C. (2017) Xylaria spinulosa sp. nov. and X. atrosphaerica from southern China. Mycosphere 8 (8): 1070–1079. http://doi.org/10.5943/mycosphere/8/8/8
Li, Y., Vasilyeva, L. & Ma, H.X. (2013) The genus Xylaria in the south of China-7. two penzigioid Xylaria species. Sydowia 65 (2): 329–335.
Liu, Y.L., Whelen, S. & Hall, B.D. (1999) Phylogenetic relationships among ascomycetes: evidence from an RNA polymerase II subunit. Molecular Biology and Evolution 16: 1799–1808. http://doi.org/10.1093/oxfordjournals.molbev.a026092
Lodge, D.J. & Cantrell, S.A. (1995) Fungal communities in wet tropical forests: variation in time and space. Canadian Journal of botany 73: 1391–1398. http://doi.org/10.1139/b95-402
Lodge, D.J., Læssøe, T., Aime, M.C. & Henkel, T.W. (2008) Montane and cloud forest specialists among neotropical Xylaria species. North American Fungi 3: 193–213. http://doi.org/10.2509/naf2008.003.00713
Ma, H.X. & Li, Y. (2017) Xylaria curta and X. partita (Xylariales) from Yunnan province. Austrian Journal of Mycology 26: 99–105.
Ma, H.X., Vasilyeva, L. & Li, Y. (2012) The genus Xylaria in the south of China–3. X. atroglobosa sp. nov. Mycotaxon 119 (1): 381–384. http://doi.org/10.5248/119.381
Ma, H.X., Vasilyeva, L. & Li, Y. (2012) The genus Xylaria in the south of China–5. Three new records in the China Mainland. Österreichische Zeitschrift für Pilzkunde 21: 61–67.
Ma, H.X., Vasilyeva, L. & Li, Y. (2013) The genus Xylaria in the south of China–4. X.hemisphaerica sp. nov. from yunnan province. Mycosystema 32 (4): 602–605.
Ma, H.X., Vasilyeva, L. & Li, Y. (2013) The genus Xylaria (Xylariaceae) in the south of China–6. a new Xylaria species based on morphological and molecular characters. Phytotaxa 147 (2): 48–54. http://dx.doi.org/10.11646/phytotaxa.147.2.2
Ma, H.X., Vasilyeva, L. & Li, Y. (2013) Xylaria in southern China–8. X. bannaensis sp nov and X. brunneovinosa new to the mainland. Mycotaxon 125 (1): 251–256. http://dx.doi.org/10.5248/125.251
Ma, H.X., Vasilyeva, L. & Li, Y. (2017) Xylaria nelumboniformis sp. nov. from tropical China. Mycotaxon 132 (2): 299–304. https://doi.org/10.5248/132.299
Ma, H.X., Qu, Z. & Li, Y. (2018) Three species of bambusicolous xylariaceous fungi from southern China. Journal of Fungal Research 16 (3): 182–190. https://doi.org/10.13341/j.jfr.2018.1225
Maharachchikumbura, S.S.N., Hyde, K.D., Jones, E.B.G., McKenzie, E.H.C., Bhat, J.D., Dayarathne, M.C., Huang, S.K., Norphanphoun, C., Senanayake, I.C., Perera, R.H., Shang, Q.J., Xiao, Y.P., D’souza, M.J., Hongsanan, S., Jayawardena, R.S., Daranagama, D.A., Goonasekara, I.D., Zhuang, W.Y., Jeewon, R., Phillips, A.J.L., Abdel-Wahab, M.A., Al-Sadi, A.M., Bahkali, A.H., Boonmee, S., Boonyuen, N., Cheewangkoon, R., Dissanayake, A.J., Kang, J.C., Li, Q.R., Liu, J.K., Liu, X.Z., Liu, Z.Y., Luangsa-ard, J.J., Pang, K.L., Phookamsak, R., Promputtha, I., Suetrong, S., Stadler, M., Wen, T.C. & Wijayawardene, N.N. (2016) Families of Sordariomycetes. Fungal Diversity 79: 1–31. https://doi.org/10.1007/s13225-016-0369-6
Nylander, J.A.A. (2004) MrModeltest v2.2. Program distributed by the author: 2. Evolutionary Biology Centre, Uppsala University: 1–2.
Peršoh, D., Melcher, M., Graf, K., Fournier, J., Stadler, M. & Rambold, G. (2009) Molecular and morphological evidence for the delimitation of Xylaria hypoxylon. Mycologia 101: 256–268. https://doi.org/10.3852/08–108
Peršoh, D., Melcher, M., Graf, K., Fournier, J., Stadler, M. & Rambold, G. (2017) Molecular and morphological evidence for the delimitation of Xylaria hypoxylon. Mycologia 101 (2): 256–268. https://doi.org/10.3852/08-108
Rambaut, A. (2006) FIGURETree. Tree FIGUREURE drawing tool version 1.4. 0. University of Edinburgh: Institute of Evolutionary Biology.
Rogers, J.D. (1979) The Xylariaceae: systematic, biological and evolutionary aspects. Mycologia 71: 1–42.
Rogers, J.D. (1983) Xylaria bulbosa, Xylaria curta, and Xylaria longipes in continental United States. Mycologia 75 (3): 457–467. https://doi.org/10.1080/00275514.1983.12023709
Rogers, J.D. (1984) Xylaria cubensis and its anamorph Xylocoremium flabelliforme, Xylaria allantoidea, and Xylaria poitei in continental United States. Mycologia 76: 912–923. https://doi.org/10.1080/00275514.1984.12023929
Rogers, J.D., Callan, B.E. & Samuels, G.J. (1987) The Xylariaceae of the rain forests of north Sulawesi (Indonesia). Mycotaxon 29: 113–172. https://doi.org/10.1007/BF00436682
Rogers, J.D. & Ju, Y.M. (1998) The genus Kretzschmaria. Mycotaxon 68: 369.
Rogers, J.D., Ju, Y.M. & Hemmes, D. (1992) Hypoxylon rectangulosporum sp. nov., Xylaria psidii sp. nov., and comments on taxa of Podosordaria and Stromatoneurospora. Mycologia 84: 166–172. https://doi.org/10.2307/3760247
Rogers, J.D., Ju, Y.M. & Ehmann, J.L. (2005) Some Xylaria species on termite nests. Mycologia 97: 914–923. https://doi.org/10.1080/15572536.2006.11832783
Rogers, J.D., Martín, F.S. & Ju, Y.M. (2002) A reassessment of the Xylaria on Liquidambar fruits and two new taxa on Magnolia fruits. Sydowia 54: 91–97.
San Martín, G.F. & Rogers, J.D. (1989) A preliminary account of Xylaria of Mexico. Mycotaxon 34 (2): 368.
Senanayake, I.C., Maharachchikumbura, S.S.N., Hyde, K.D., Bhat, D.J., Gareth, Jones, E.B., McKenzie, E.H.C., Dai, D.Q., Daranagama, D.A., Dayarathne, M., Goonasekara, I., Konta, S., Li, W.J., Shang, Q.J., Stadler, M., Wijayawardene, N.N., Xiao, Y.P., Norphanphoun, C, Li, Q.R., Liu, X.Z., Bahkali, A., Kang, J.C., Wang, Y., Wen, T.C., Wendt, L., Xu, J.C. & Erio, C. (2015) Towards unraveling relationships in Xylariomycetidae (Sordariomycetes). Fungal Diversity 73: 73–144. https://doi.org/10.1007/s13225-015-0340-y
Sodngam, S., Sawadsitang, S., Suwannasai, N. & Mongkolthanaruk, W. (2014) Chemical constituents, and their cytotoxicity, of the rare wood decaying fungus Xylaria humosa. Natural Product Communications 2 (9): 157–158. https://doi.org/10.1021/np5001208
Stadler, M., Kuhnert, E., Persoh, D. & Fournier, J. (2013) The Xylariaceae as model example for a unified nomenclature following the “One Fungus-One Name” (1F1N) concept. Mycology 4: 5–21. http:doi.org/10.1080/21501203.2013.782478
Stadler, M., Wollweber, H., Jäger, W., Briegert, M., Venturella, G., Castro, J.M. & Tichy, H.V. (2004) Cryptic species related to Daldinia concentrica and D. eschscholzii, with notes on D. bakeri. Mycological Research 108: 257–273.
Tanaka, K., Hirayama, K., Yonezawa, H., Hatakeyama, S., Harada, Y., Sano, T., Shirouzu, T. & Hosoya, T. (2009) Molecular taxonomy of bambusicolous fungi: Tetraplosphaeriaceae, a new pleosporalean family with Tetraploa-like anamorphs. Studies in Mycology 64: 175–209. https://doi.org/10.3114/sim.2009.64.10
Tapia, F., Ju, Y.M., Chacón, S. & Barillas, E.U. (2017) Five penzigioid Xylaria species from Veracruz (Mexico). Mycotaxon 132 (1): 19–27. http://dx.doi.org/10.5248/132.19
Trierveiler-Pereira, L., Romero, A.I., Baltazar, J.M. & Loguercio-Leite, C. (2009) Addition to the knowledge of Xylaria (Xylariaceae, Ascomycota) in santa catarina, southern brazil. Mycotaxon 107 (1): 139–156. https://doi.org/10.5248/107.139
Voglmayr, H., Friebes, G., Gardiennet, A. & Jaklitsch, W.M. (2018) Barrmaelia and Entosordaria in Barrmaeliaceae (fam. nov., Xylariales) and critical notes on Anthostomella-like genera based on multigene phylogenies. Mycological Progress 17: 155–177. https://doi.org/10.1007/s11557-017-1329-6
Vu, D., Groenewald, M., de Vries, M., Gehrmann, T., Stielow, B., Eberhardt, U., Al-Hatmi, A., Groenewald, J.Z., Cardinali, G., Houbraken, J., Boekhout, T., Crous, P.W., Robert, V. & Verkley, G.J.M. (2019) Large-scale generation and analysis of filamentous fungal DNA barcodes boosts coverage for kingdom fungi and reveals thresholds for fungal species and higher taxon delimitation. Studies in Mycology 92: 135–154. https://doi.org/10.1016/j.simyco.2018.05.001
Wendt, L., Sir, E.B., Kuhnert, E., Heitkämper, S., Lambert, C., Hladki, A.I., Romero, A.I., Luangsa-ard, J.J., Srikitikulchai, P., Peršoh, D. & Stadler, M. (2018) Resurrection and emendation of the Hypoxylaceae, recognised from a multigene phylogeny of the Xylariales. Mycological Progress 17: 115–154. https://doi.org/10.13119/100010665
Whalley, A.J.S. (1996) The Xylariaceous way of life. Mycologocal Research 100: 897–922. https://doi.org/10.1016/S0953-7562(96)80042-6
White, T.J., Bruns, T., Lee, S., Taylor, F., Lee, S.H., Taylor, L. & Shawe-Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocols: a guide to methods and applications. Academic Press, San Diego, California, pp. 315–322.
Wijayawardene, N.N., Dissanayake, L.S., Li, Q.R., Dai, D.Q., Xiao, Y.P., Wen, T.C., Karunarathna, S.C., Wu, H.X., Zhang, H., Tibpromma, S., Kang, J.C., Wang, Y., Shen, X.C., Tang, L.Z., Deng, C.Y., Liu, Y.X. & Kang, Y.Q. (2021) Yunnan–Guizhou Plateau: a mycological hotspot. Phytotaxa 523 (1): 1–31. https://doi.org/10.11646/phytotaxa.523.1.1
Winter, G. (1887) Fungi novi Brasilienses. Grevillea 15 (75): 89.
Yang, X.L., Liu, J.K., Luo, D.Q. & Zhang, S. (2011) Chemical constituents of Xylaria nigripe. Natural Product Research & Development 5: 846–849. https://doi.org/10.16333/j.1001-6880.2011.05.012
Zhu, Y.F. & Guo, L. (2011) Xylaria hainanensis sp. nov. (Xylariaceae) from China. Mycosystema 30 (4): 526–528. https://doi.org/10.13346/j.mycosystema.2011.04.014
