Abstract
Species of Boletellus Section Chrysenteroidei are widely distributed in the world, especially in the subtropical to tropical regions. By means of molecular techniques, the species diversity of this section has become clearer and new taxa have been uncovered. In this study, a new species of this section from the southeast Yunnan of China, namely Boletellus wenshanensis, was identified based on molecular and morphological evidence. This species can be distinguised by the combination of morphological characters: a quick dark blue reaction on all parts of the basidiome when bruised, a brown to brownish orange pileus uncracking with age, a scaly stipe covered with brownish punctate squamules, yellow hymenophore, interwoven pileipellis, ellipsoid to broadly fusoid basidiospores ornamented with longitudinal ridges, and a hyphohymeniform stipitipellis. The new species is presented with illustrated description and comparisons with allied species.
References
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<p>Snell, W.H. (1941) The genera of the Boletaceae. <em>Mycologia</em> 33 (4): 415–423. https://doi.org/10.2307/3754896</p>
<p>Stamatakis, A. (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. <em>Bioinformatics</em> 30 (9): 1312–1313. https://doi.org/10.1093/bioinformatics/btu033</p>
<p>Thiers, B. (2020) Index Herbariorum: A global directory of public herbaria and associated staff. New York Botanical Garden’s Virtual Herbarium. Available from: http://sweetgumnybgorg/science/ih/ (accessed 18 May 2022)</p>
<p>Vadthanarat, S., Raspé, O. & Lumyong, S. (2018) Phylogenetic affinities of the sequestrate genus <em>Rhodactina</em> (Boletaceae), with a new species, <em>R. rostratispora</em> from Thailand. <em>Mycokeys</em> 29: 63–80. https://doi.org/10.3897/mycokeys.29.22572</p>
<p>Wen, H.-A. (1985) New species and new records of genus <em>Boletellus</em> from China. <em>Acta Mycologica Sinica</em> 4 (4): 222–226.</p>
<p>Wu, G., Feng, B., Xu, J.-P., Zhu, X.-T., Li, Y.-C., Zeng, N.-K., Hosen, M.I. & Yang, Z.-L. (2014) Molecular phylogenetic analyses redefine seven major clades and reveal 22 new generic clades in the fungal family Boletaceae. <em>Fungal Diversity</em> 69: 93–115. https://doi.org/10.1007/s13225-014-0283-8</p>
<p>Wu, G., Li, Y.-C., Zhu, X.-T., Zhao, K., Han, L.-H., Cui, Y.-Y., Li, F., Xu, J.-P. & Yang, Z.-L. (2016) One hundred noteworthy boletes from China. <em>Fungal Diversity</em> 81: 25–188. https://doi.org/10.1007/s13225-016-0375-8</p>
<p>Zang, M. (1985) Notes on the Boletales from eastern Himalayas and adjacent of China. <em>Acta Botanica Yunnanica</em> 7 (4): 383–401.</p>
<p>Zang, M., Chen, S.M. & Sittigul, C. (1999) Some new and interesting taxa of Boletales from tropical Asia. <em>Fungal Science</em> 14 (1–2): 19–25.</p>
<p>Zhang, W., Wang, D., Yang, S.-F., Long, C.-H., Wang, L., Zhu, W., Jiang, S.-W. & Yan, Z.-H. (2011) The characteristics of weather and climate in Wenshan and the focus of severe weather forecast (in Chinese). <em>Yunnan Geographic Environment Research</em> 23: 77–81.</p>
<p>Fulgenzi, T.D., Mayor, J.R., Henkel, T.W. & Halling, R.E. (2008) New species of <em>Boletellus</em> from Guyana. <em>Mycologia</em> 100 (3): 490–495. https://doi.org/10.3852/07-170R</p>
<p>Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. <em>Nucleic Acids Symposium Series</em> 41: 95–98.</p>
<p>Hongo, T. & Izawa, M. (1994) <em>Yama-Kei Field Book on Mushrooms. </em>Yama-Kei Publishers Co. Ltd., Tokyo.</p>
<p>Horak, E. (1977) <em>Boletellus</em> and <em>Porphyrellus</em> in Papua New Guinea. <em>Kew Bulletin</em> 31 (3): 645–652. https://doi.org/10.2307/4119416</p>
<p>Katoh, K. & Standley, D.M. (2013) MAFFT Multiple sequence alignment software version 7: Improvements in performance and usability. <em>Molecular Biology and Evolution</em> 30 (4): 772–780. https://doi.org/10.1093/molbev/mst010</p>
<p>Kornerup, A. & Wanscher, J.H. (1981) <em>Taschenlexikon der Farben, 3rd edn</em>. Aufl. Göttingen: Muster-Schmidt Verlag.</p>
<p>Lanfear, R., Frandsen, P.B., Wright, A.M., Senfeld, T. & Calcott, B. (2017) PartitionFinder 2: New methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses. <em>Molecular Biology & Evolution</em> 34 (3): 772–773. https://doi.org/10.1093/molbev/msw260</p>
<p>Li, Y.-C., Yang, Z.-L. & Bau, T. (2009) Phylogenetic and biogeographic relationships of <em>Chroogomphus</em> species as inferred from molecular and morphological data. <em>Fungal Diversity </em>38: 85–104.</p>
<p>Mayor, J.R., Fulgenzi, T.D., Henkel, T.W. & Halling, R.E. (2008) <em>Boletellus piakaii </em>sp. nov. and a new distribution record for <em>Boletellus ananas</em> var. <em>ananas</em> from Guyana. <em>Mycotaxon</em> 105: 387–398.</p>
<p>Nuhn, M.E., Binder, M., Taylor, A.F.S., Halling, R.E. & Hibbett, D.S. (2013) Phylogenetic overview of the Boletineae. <em>Fungal Biology</em> 117 (7–8): 479–511. https://doi.org/10.1016/j.funbio.2013.04.008</p>
<p>Ortiz-Santana, B., Lodge, D.J., Baroni, T.J. & Both, E.E. (2007) Boletes from Belize and the Dominican Republic. <em>Fungal Diversity</em> 27: 247–416.</p>
<p>Parihar, A., Hembrom, M.E., Vizzini, A. & Das, K. (2018) A new species of <em>Boletellus</em> (Boletaceae, Basidiomycota) from tropical India. <em>Nordic Journal of Botany</em> 36. https://doi.org/10.1111/njb.02089</p>
<p>Ronquist, F., Teslenko, M., Van Der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. <em>Systematic Biology</em> 61 (3): 539–542. https://doi.org/10.1093/sysbio/sys029</p>
<p>Singer, R. (1945) The <em>Boletineae </em>of Florida with notes on extralimital species. I. Strobilomycetaceae. <em>Farlowia</em> 2 (1): 97–141. https://doi.org/10.5962/p.316006</p>
<p>Smith, A.H. & Thiers, H.D. (1971) <em>The boletes of Michigan.</em> University of Michigan Press, Ann Arbor.</p>
<p>Smith, S.A. & Dunn, C.W. (2008) Phyutility: a phyloinformatics tool for trees, alignments and molecular data. <em>Bioinformatics</em> 24 (5): 715–716. https://doi.org/10.1093/bioinformatics/btm619</p>
<p>Snell, W.H. (1936) Notes on boletes. V. <em>Mycologia</em> 28 (5): 463–475. https://doi.org/10.1080/00275514.1936.12017161</p>
<p>Snell, W.H. (1941) The genera of the Boletaceae. <em>Mycologia</em> 33 (4): 415–423. https://doi.org/10.2307/3754896</p>
<p>Stamatakis, A. (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. <em>Bioinformatics</em> 30 (9): 1312–1313. https://doi.org/10.1093/bioinformatics/btu033</p>
<p>Thiers, B. (2020) Index Herbariorum: A global directory of public herbaria and associated staff. New York Botanical Garden’s Virtual Herbarium. Available from: http://sweetgumnybgorg/science/ih/ (accessed 18 May 2022)</p>
<p>Vadthanarat, S., Raspé, O. & Lumyong, S. (2018) Phylogenetic affinities of the sequestrate genus <em>Rhodactina</em> (Boletaceae), with a new species, <em>R. rostratispora</em> from Thailand. <em>Mycokeys</em> 29: 63–80. https://doi.org/10.3897/mycokeys.29.22572</p>
<p>Wen, H.-A. (1985) New species and new records of genus <em>Boletellus</em> from China. <em>Acta Mycologica Sinica</em> 4 (4): 222–226.</p>
<p>Wu, G., Feng, B., Xu, J.-P., Zhu, X.-T., Li, Y.-C., Zeng, N.-K., Hosen, M.I. & Yang, Z.-L. (2014) Molecular phylogenetic analyses redefine seven major clades and reveal 22 new generic clades in the fungal family Boletaceae. <em>Fungal Diversity</em> 69: 93–115. https://doi.org/10.1007/s13225-014-0283-8</p>
<p>Wu, G., Li, Y.-C., Zhu, X.-T., Zhao, K., Han, L.-H., Cui, Y.-Y., Li, F., Xu, J.-P. & Yang, Z.-L. (2016) One hundred noteworthy boletes from China. <em>Fungal Diversity</em> 81: 25–188. https://doi.org/10.1007/s13225-016-0375-8</p>
<p>Zang, M. (1985) Notes on the Boletales from eastern Himalayas and adjacent of China. <em>Acta Botanica Yunnanica</em> 7 (4): 383–401.</p>
<p>Zang, M., Chen, S.M. & Sittigul, C. (1999) Some new and interesting taxa of Boletales from tropical Asia. <em>Fungal Science</em> 14 (1–2): 19–25.</p>
<p>Zhang, W., Wang, D., Yang, S.-F., Long, C.-H., Wang, L., Zhu, W., Jiang, S.-W. & Yan, Z.-H. (2011) The characteristics of weather and climate in Wenshan and the focus of severe weather forecast (in Chinese). <em>Yunnan Geographic Environment Research</em> 23: 77–81.</p>