Abstract
Xenoastrosphaeriella aquatica sp. nov. was discovered during an investigation of lignicolous freshwater fungi in northwestern Yunnan Province, China. Evidence of morphological characters and sequence data indicated that the new collection has affiliation to Xenoastrosphaeriella. Phylogenetic analysis of combined LSU, SSU and tef1-α sequence data was carried out to determine the phylogenetic placement of this species, which showed that X. aquatica clustered with X. tornata and X. trochus, but these three species are phylogenetically distinct. Xenoastrosphaeriella aquatica differs from other species in having dark olive to brown and larger ascospores. A detailed description and illustration of the new species are provided, as well as the comparisons with similar taxa.
References
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Nylander, J.A.A. (2004) MrModeltest 2.0. Program distributed by the author. Evolutionary Biology Centre, Uppsala University.
Phookamsak, R., Norphanphoun, C., Tanaka, K., Dai, D.Q., Luo, Z.L., Liu, J.K., Su, H.Y., Bhat, D.J., Bahkali, A.H., Mortimer, P.E., Xu, J.C. & Hyde, K.D. (2015) Towards a natural classification of Astrosphaeriella-like species; introducing Astrosphaeriellaceae and Pseudoastrosphaeriellaceae fam. nov. and Astrosphaeriellopsis, gen. nov. Fungal Diversity 74: 143–197. https://doi.org/ 10.1007/s13225-015-0352-7
Rambaut, A. (2012) FigTree version 1.4.0. Available at http://tree.bio.ed.ac.uk/software/figtree (accessed 28 April 2022)
Rehner, S.A. & Buckley, E. (2005) A Beauveria phylogeny inferred from nuclear ITS and EF1-? sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 97: 84–98. http://dx.doi.org/10.1080/15572536.2006.11832842
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Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
Wanasinghe, D.N., Jeewon, R., Jones, E.B.G., Boonmee, S., Kaewchai, S., Manawasinghe, I.S., Lumyong, S. & Hyde, K.D. (2018) Novel palmicolous taxa within Pleosporales: multigene phylogeny and taxonomic circumscription. Mycological Progress 17: 571–590. https://doi.org/10.1007/s11557-018-1379-4
White, T.J., Bruns, T.D., Lee, S.B. & Taylor, J.W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols: A Guide to Methods and Applications: 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
Wijayawardene, N.N., Hyde, K.D., Lumbsch, H.T., Liu, J.K., Maharachchikumbura, S.S.N., Ekanayaka, A.H., Tian, Q. & Phookamsak, R. (2018) Outline of Ascomycota: 2017. Fungal Diversity 88: 167–263. https://doi.org/10.1007/s13225-018-0394-8
Zhang, Y., Crous, P.W., Schoch, C.L. & Hyde, K.D. (2012) Pleosporales. Fungal Diversity 53: 1–221. https://doi.org/10.1007/s13225-011-0117-x
Boise, J.R. (1984) New and interesting fungi (Loculoascomycetes) from the Amazon. Acta Amazonica 14: 49–53. https://doi.org/10.1590/1809-43921984145053
Dissanayake, A.J., Bhunjun, C.S., Maharachchikumbura, S.S.N. & Liu, J.K. (2020) Applied aspects of methods to infer phylogenetic relationships amongst fungi. Mycosphere 11: 2653–2677. https://doi.org/10.5943/mycosphere/11/1/18
Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.
Hawksworth, D.L. (1981) Astrosphaeriella Sydow, a misunderstood genus of melanommataceous Pyrenomycetes. Botanical Journal of the Linnean Society 82: 35–59.
Hawksworth, D. & Boise, J. (1985) Some additional species of Astrosphaeriella, with a key to the members of the genus. Sydowia 38: 114–124.
Hongsanan, S., Hyde, K.D., Phookamsak, R., Wanasinghe, D.N., McKenzie, E.H.C., Sarma, V.V., Boonmee, S., Lücking, R., Pem, D., Bhat, J.D., Liu, N.G., Tennakoon, D.S., Karunarathna, A., Jiang, S.H., Jones, E.B.G., Phillips, A.J.L., Manawasinghe, I., Tibpromma, S., Jayasiri, S.C., Sandamali, D., Jayawardena, R.S., Wijayawardene, N.N., Ekanayaka, A.H., Jeewon, R., Lu, Y.Z., Dissanayake, A.J., Luo, Z.L., Tian, Q., Phukhamsakda, C., Thambugala, K.M., Dai, D.Q., Chethana, T.K.W., Ertz, D., Doilom, M., Liu, J.K., Pérez-Ortega, S., Suija, A., Senwanna, C., Wijesinghe, S.N., Konta, S., Niranjan, M., Zhang, S.N., Ariyawansa, H.A., Jiang, H.B., Zhang, J.F., de Silva, N.I., Thiyagaraja, V., Zhang, H., Bezerra, J.D.P., Miranda-Gonzáles, R., Aptroot, A., Kashiwadani, H., Harishchandra, D., Aluthmuhandiram, J.V.S., Abeywickrama, P.D., Bao, D.F., Devadatha, B., Wu, H.X., Moon, K.H., Gueidan, C., Schumm, F., Bundhun, D., Mapook, A., Monkai, J., Chomnunti, P., Samarakoon, M.C., Suetrong, S., Chaiwan, N., Dayarathne, M.C., Yang, J., Rathnayaka, A.R., Bhunjun, C.S., Xu, J.C., Zheng, J.S., Liu, G., Feng, Y. & Xie, N. (2020) Refined families of Dothideomycetes: Dothideomycetidae and Pleosporomycetidae. Mycosphere 11: 1553–2107. https://doi.org/10.5943/mycosphere/11/1/13
Hyde, K.D. & Fröhlich, J. (1998) Fungi from palms XXXVII. The genus Astrosphaeriella, including ten new species. Sydowia 50: 81–132.
Jayasiri, S.C., Hyde, K.D., Abd-Elsalam, K.A., Abdel-Wahab, M.A., Ariyawansa, H.A., Bhat, J.D., Buyck, B., Dai, Y.C., Ertz, D., Hidayat, I., Jeewon, R., Jones, E.B.G., Karunarathna, S.C., Kirk, P., Lei, C., Liu, J.K., Maharachchikumbura, S.S.N., McKenzie, E., Ghobad-Nejhad, M., Nilsson, H., Pang, K.L., Phookamsak, R., Rollins, A.W., Romero, A.I., Stephenson, S., Suetrong, S., Tsui, C.K.M., Vizzini, A., Wen, T.C., De Silva, N.I., Promputtha, I. & Kang, J.C. (2015) The Facesoffungi database: fungal names linked with morphology, molecular and human attributes. Fungal Diversity 74: 3–18. https://doi.org/10.1007/s13225-015-0351-8
Jayasiri, S.C., Hyde, K.D., Jones, E.B.G., McKenzie, E.H.C., Jeewon, R., Phillips, A.J.L., Bhat, D.J., Wanasinghe, D.N., Liu, J.K., Lu, Y.Z., Kang, J.C., Xu, J.C. & Karunarathna, S.C. (2019) Diversity, morphology and molecular phylogeny of Dothideomycetes on decaying wild seed pods and fruits. Mycosphere 10: 1–186 https://doi.org/10.5943/mycosphere/10/1/1
Katoh, K. & Standley, D.M. (2016) A simple method to control over-alignment in the MAFFT multiple sequence alignment program. Bioinformatics 32: 1933–1942. https://doi.org/10.1093/bioinformatics/btw108
Larsson, A. (2014) AliView: a fast and lightweight alignment viewer and editor for large data sets. Bioinformatics 30: 3276–3278. https://doi.org/10.1093/bioinformatics/btu531
Liu, L.L., Long, Q.D., Kang, J.C., Zhang, X., Hyde, K.D., Shen, X.C. & Li, Q.R. (2018) Morphology and phylogeny of Mycopepon. Mycosphere 9: 779–789. https://doi.org/10.5943/mycosphere/9/4/6
Luo, Z.L., Hyde, K.D., Liu, J.K., Bhat, D.J., Bao, D.F., Li, W.L., Su, X.J., Yang, X.Y. & Su, H.Y. (2018) Lignicolous freshwater fungi from China II: novel Distoseptispora (Distoseptisporaceae) species from northwestern Yunnan Province and a suggested unified method for studying lignicolous freshwater fungi. Mycosphere 9: 444–461. https://doi: 10.5943/mycosphere/9/3/2
Nylander, J.A.A. (2004) MrModeltest 2.0. Program distributed by the author. Evolutionary Biology Centre, Uppsala University.
Phookamsak, R., Norphanphoun, C., Tanaka, K., Dai, D.Q., Luo, Z.L., Liu, J.K., Su, H.Y., Bhat, D.J., Bahkali, A.H., Mortimer, P.E., Xu, J.C. & Hyde, K.D. (2015) Towards a natural classification of Astrosphaeriella-like species; introducing Astrosphaeriellaceae and Pseudoastrosphaeriellaceae fam. nov. and Astrosphaeriellopsis, gen. nov. Fungal Diversity 74: 143–197. https://doi.org/ 10.1007/s13225-015-0352-7
Rambaut, A. (2012) FigTree version 1.4.0. Available at http://tree.bio.ed.ac.uk/software/figtree (accessed 28 April 2022)
Rehner, S.A. & Buckley, E. (2005) A Beauveria phylogeny inferred from nuclear ITS and EF1-? sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 97: 84–98. http://dx.doi.org/10.1080/15572536.2006.11832842
Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61 (3): 539–542. https://dx.doi.org/10.1093/sysbio/sys029
Senanayake, I.C., Rathnayaka, A.R., Marasinghe, D.S., Calabon, M.S., Gentekaki, E., Wanasinghe, D.N., Lee, H.B., Hurdeal, V.G., Pem, D., Dissanayake, L.S., Wijesinghe, S.N., Bundhun, D., Nguyen, T.T., Goonasekara, I.D., Abeywickrama, P.D., Bhunjun, C.S., Chomnunti, P., Boonmee, S., Jayawardena, R.S., Wijayawardene, N.N., Doilom, M., Jeewon, R., Bhat, J.D., Zhang, H.X. & Xie, N. (2020) Morphological approaches in studying fungi: collection, examination, isolation, sporulation and preservation. Mycosphere 11: 2678–2754. https://doi10.5943/mycosphere/11/1/20
Silvestro, D. & Michalak, I. (2012) RaxmlGUI: a graphical front-end for RAxML. Organisms Diversity & Evolution 12: 335–337. https://doi.org/10.1007/s13127-011-0056-0
Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution 30: 2725–2729. https://doi.org/10.1093/molbev/mst197
Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
Wanasinghe, D.N., Jeewon, R., Jones, E.B.G., Boonmee, S., Kaewchai, S., Manawasinghe, I.S., Lumyong, S. & Hyde, K.D. (2018) Novel palmicolous taxa within Pleosporales: multigene phylogeny and taxonomic circumscription. Mycological Progress 17: 571–590. https://doi.org/10.1007/s11557-018-1379-4
White, T.J., Bruns, T.D., Lee, S.B. & Taylor, J.W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols: A Guide to Methods and Applications: 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
Wijayawardene, N.N., Hyde, K.D., Lumbsch, H.T., Liu, J.K., Maharachchikumbura, S.S.N., Ekanayaka, A.H., Tian, Q. & Phookamsak, R. (2018) Outline of Ascomycota: 2017. Fungal Diversity 88: 167–263. https://doi.org/10.1007/s13225-018-0394-8
Zhang, Y., Crous, P.W., Schoch, C.L. & Hyde, K.D. (2012) Pleosporales. Fungal Diversity 53: 1–221. https://doi.org/10.1007/s13225-011-0117-x