Abstract
Tylopilus yangzhuliangii (Boletaceae, Boletales) is described as a new species, based on morphological and phylogenetic evidence, from Hainan Province, a tropical region of southern China. A detailed description, color photos of fresh basidiomes, and line-drawings of microstructures of the new species are presented as well as comparisons with related species.
References
Baroni, T.J. & Both, E.E. (1998) Tylopilus violatinctus, a new species of Tylopilus for North America, with comments on other violaceous colored Tylopilus taxa. Bulletin of the Buffalo Society of Natural Sciences 36: 261–264.
Bau, T., Bao, H.Y. & Li, Y. (2014) A revised checklist of poisonous mushrooms in China. Mycosystema 33 (3): 517–548. https://doi.org/10.13346/j.mycosystema.130256
Binder, M. & Besl, H. (2000) 28S rDNA sequence data and chemotaxonomical analyses on the generic concept of Leccinum (Boletales). Mycologia 75–86.
Binder, M. & Hibbett, D.S. (2006) Molecular systematics and biological diversification of Boletales. Mycologia 98: 971–981. https://doi.org/10.1080/15572536.2006.11832626
Bulliard, J.B.F. (1788) Herbier de la France 8. Privately published, Paris. pp. 337–384.
Castresana, J. (2000) Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Molecular Biology and Evolution 17: 540–552. https://doi.org/10.1093/oxfordjournals.molbev.a026334
Chai, H., Liang, Z.Q., Xue, R., Jiang, S., Luo, S.H., Wang, Y., Wu, L.L., Tang, L.P., Chen, Y., Hong, D. & Zeng, N.K. (2019) New and noteworthy boletes from subtropical and tropical China. Mycokeys 46: 55–96. https://doi.org/10.3897/mycokeys.46.31740
Chakraborty, D., Vizzini, A. & Das, K. (2018) Two new species and one new record of the genus Tylopilus (Boletaceae) from Indian Himalaya with morphological details and phylogenetic estimations. MycoKeys 33: 103–124. https://dx.doi.org/10.3897%2Fmycokeys.33.23703
Corner, E.J.H. (1972) Boletus in Malaysia. Government Printer, Singapore, 263 pp.
Corrales, A., Turner, B.L., Tedersoo, L., Anslan, S. & Dalling, J.M. (2017) Nitrogen addition alters ectomycorrhizal fungal communities and soil enzyme activities in a tropical montane forest. Fungal Ecology 27: 14–23. https://doi.org/10.1016/j.funeco.2017.02.00
Dentinger, B.T., Ammirati, J.F., Both, E.E., Desjardin, D.E., Halling, R.E., Henkel, T.W., Moreau, P.A., Nagasawa, E., Soytong, K., Taylor, A.F., Watling, R., Moncalvo, J.M. & McLaughlin, D.J. (2010) Molecular phylogenetics of porcini mushrooms (Boletus section Boletus). Molecular Phylogenetics and Evolution 57: 1276–1292. https://dx.doi.org/10.1016/j.ympev.2010.10.00
Edgar, R.C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32: 1792–1797. https://doi.org/10.1093/nar/gkh340
Fu, S.Z., Wang, Q.B. & Yao, Y.J. (2006) Tylopilus microsporus, a new species from Southwest China. Mycotaxon 96: 41–46.
Fulgenzi, T.D., Henkel, T.W. & Halling, R.E. (2007) Tylopilus orsonianus sp. nov. and Tylopilus eximius from Guyana. Mycologia 99: 622–627. https://dx.doi.org/10.1080/15572536.2007.11832556
Gelardi, M., Angelini, C., Costanzo, F., Dovana, F., Santana, B.O. & Vizzini, A. (2019) Tylopilus griseiolivaceus sp. nov. and T. leucomycelinus (Boletaceae) revisited from the Dominican Republic within a comprehensive phylogeny of Tylopilus s. str. Mycological Progress 18: 1039–1056.
Gelardi, M., Vizzini, A., Ercole, E., Taneyama, Y., Li, T.H., Zhang, M., Yan, W.J. & Wang, W.J. (2015) New collection, iconography and molecular evidence for Tylopilus neofelleus (Boletaceae, Boletoideae) from southwestern China and the taxonomic status of T. plumbeoviolaceoides and T. microsporus. Mycoscience 56: 373–386. https://dx.doi.org/10.1016/j.myc.2014.12.001
Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98. http://doi.org/10.14601/Phytopathol_Mediterr-14998u1.29
Halling, R.E., Nuhn, M., Osmundson, T., Fechner, N., Trappe, J.M., Soytong, K., Arora, D., Hibbett, D.S. & Binder, M. (2012) Affinities of the Boletus chromapes group to Royoungia and the description of two new genera, Harrya and Australopilus. Australian Systematic Botany 25: 418–431. https://doi.org/10.1071/SB12028
Halling, R.E., Osmundson, T.W. & Neves, M.A. (2008) Pacific boletes: implications for biogeographic relationships. Mycological Research 112: 437–447. https://doi.org/10.1016/j.mycres.2007.11.021
Henkel, T.W. (1999) New taxa and distribution records for Tylopilus from Dicymbe forests of Guyana. Mycologia 91: 655–665. https://dx.doi.org/10.2307/3761252
Hongo, T. (1964) Notulae mycologicae (3). Memoirs of Faculty Education of Shiga University, Natural Sciences 14: 43–47.
Hongo, T. (1966) Notulae mycologicae (5). Memoirs of the Faculty of Education of Shiga University, Natural Sciences 16: 57–62.
Hongo, T. (1967) Notes on Japanese larger fungi (19). Journal of Japanese Botany 42: 151–159.
Horak, E. (2011) Revision of Malaysian Species of Boletales s.l. (Basidiomycota) Described by E.J.H. Corner (1972, 1974). Malayan Forest Records 51: 1–283.
Huelsenbeck, J.P. & Ronquist, F. (2005) Bayesian analysis of molecular evolution using MrBayes. In: Nielsen, R. (Ed.) Statistical methods in molecular evolution. Springer, New York, pp. 183–226. https://doi.org/10.1007/0-387-27733-1_7
James, T.Y., Kauff, F., Schoch, C., Matheny, P.B., Hofstetter, V., Cox, C., Celio, G., Gueidan, C., Fraker, E., Miadlikowska, J., Lumbsch, H.T., Rauhut, A., Reeb, V., Arnold, A.E., Amtoft, A., Stajich, J.E., Hosaka, K., Sung, G.H., Johnson, D., O’Rourke, B., Crockett, M., Binder, M., Curtis, J.M., Slot, J.C., Wang, Z., Wilson, A.W., Schüßler, A., Longcore, J.E., O’Donnell, K., Mozley-Standridge, S., Porter, D., Letcher, P.M., Powell, M.J., Taylor, J.W., White, M.M., Griffith, G.W., Davies, D.R., Humber, R.A., Morton, J.B., Sugiyama, J., Rossman, A.Y., Rogers, J.D., Pfister, D.H., Hewitt, D., Hansen, K., Hambleton, S., Shoemaker, R.A., Kohlmeyer, J., Volkmann-Kohlmeyer, B., Spotts, R.A., Serdani, M., Crous, P.W., Hughes, K.W., Matsuura, K., Langer, E., Langer, G., Untereiner, W.A., Lücking, R., Büdel, B., Geiser, D.M., Aptroot, A., Diederich, P., Schmitt, I., Schultz, M., Yahr, R., Hibbett, D., Lutzoni, F., McLaughlin, D., Spatafora, J. & Vilgalys, R. (2006) Reconstructing the early evolution of the fungi using a six gene phylogeny. Nature 443: 818–822. http://dx.doi.org/10.1038/nature05110
Jiang, H.S., Chen, B.L., Zhou, Y.D., Wang, C.D., Fang, L. & Luo, Y.K. (2013) Biodiversity and conservation of Hainan Yinggeling Nature Reserve. China Forestry Press, Beijing, 629 pp.
Karsten, P.A. (1881) Enumeratio boletinearum et polyporearum fennicarum, systemate novo dispositarum. Revue Mycologique 3: 16–19.
Kornerup, A. & Wanscher, J.H. (1981) Taschenlexikon der Farben. 3. Aufl. Muster-Schmidt Verlag, Göttingen, 242 pp.
Kuntze, O. (1898) Revisio generum plantarum 3. Arthur Felix, Dulau & Co., Leipzig, 576 pp.
Lee, S.J., Yun, B.S., Cho, D.H. & Yoo, I.D. (1999) Tylopeptins A and B, new antibiotic peptides from Tylopilus neofelleus. Journal of Bacteriology 52: 998–1006. https://doi.org/10.7164/antibiotics.52.998
Li, T.H., Song, B. & Shen, Y.H. (2002) A new species of Tylopilus from Guangdong. Mycosystema 21: 3–5.
Li, Y.C., Feng, B. & Yang, Z.L. (2011) Zangia, a new genus of Boletaceae supported by molecular and morphological evidence. Fungal Diversity 49: 125–143.
Li, Y.C. & Yang, Z.L. (2021) The boletes of China: Tylopilus s.l. Springer, Berlin, 418 pp. https://doi.org/10.1007/978-981-16-2986-0
Liang, Z.Q., Su, M.S., Jiang, S., Hong, D. & Zeng, N.K. (2018) Tylopilus callainus, a new species with a sea-green color change of hymenophore and context from the south of China. Phytotaxa 343: 269–276. https://doi.org/10.11646/phytotaxa.343.3.7
Magnago, A.C., Reck, M.A., Dentinger, B.T.M., Moncalvo, J.M., Neves, M.A. & Silveira, R.M.B.D. (2017) Two new Tylopilus species (Boletaceae) from Northeastern Atlantic Forest, Brazil. Phytotaxa 316: 250–260. https://dx.doi.org/10.11646/phytotaxa.316.3.4
Mazzer, S.J. & Smith, A.H. (1967) New and interesting boletes from Michigan. The Michigan Botanist 6: 57–67.
Nagy, L.G., Kocsubé, S., Csanádi, Z., Kovács, G.M., Petkovits, T., Vágvölgyi, C. & Papp, T. (2012) Re-mind the gap! Insertion–deletion data reveal neglected phylogenetic potential of the nuclear ribosomal internal transcribed spacer (ITS) of fungi. PLoS ONE 7: e49794. https://doi.org/10.1371/journal.pone.0049794
Nuhn, M.E., Binder, M., Taylor, A.F.S., Halling, R.E. & Hibbett, D.S. (2013) Phylogenetic overview of the Boletineae. Fungal Biology 117: 479–511. https://doi.org/10.1016/j.funbio.2013.04.008
Nylander, J.A.A. (2004) MrModeltest 2.3. program distributed by the author. Evolutionary Biology Center, Uppsala University.
Osmundson, T. & Halling, R.E. (2010) Tylopilus oradivensis sp. nov.: a newly described member of the Tylopilus balloui complex from Costa Rica. Mycotaxon 113: 475–483. https://doi.org/10.5248/113.475
Osmundson, T.W., Bougher, N.L., Robinson, R.M. & Halling, R.E. (2021) Rubinoboletus phaseolisporus (Boletaceae) from Western Australia is a Tylopilus with bean-shaped spores. Nuytsia 32: 87–97.
Pegler, D.N. & Young, T.W.K. (1981) A natural arrangement of the Boletales, with reference to spore morphology. Transactions of the British Mycological Society 76: 103–146. https://doi.org/10.1016/S0007-1536(81)80013-7
Singer, R. (1947) The Boletoideae of Florida. The Boletineae of Florida with notes on extralimital species III. The American Midland Naturalist 37: 1–135.
Singer, R. (1986) The Agaricales in modern taxonomy, 4th edn. Koeltz Scientific Books, Koenigstein, 981 pp.
Smith, S.A. & Dunn, C.W. (2008) Phyutility: a phyloinformatics tool for trees, alignments and molecular data. Bioinformation 24: 715–716. https://doi.org/10.1093/bioinformatics/btm619
Smith, M.E., Henkel, T.W., Aime, M.C., Fremier, A.K. & Vilgalys, R. (2011) Ectomycorrhizal fungal diversity and community structure on three co-occurring leguminous canopy tree species in a neotropical rainforest. New Phytologist 192: 699–712. https://doi.org/10.1111/j.1469-8137.2011.03844.
Stamatakis, A. (2006) RAxML-VI-HPC: maximum likelihood based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22: 2688–2690. https://doi.org/10.1093/bioinformatics/btl446
Terashima, Y., Takahashi, H. & Taneyama, Y. (2016) The fungal flora in Southwestern Japan: agarics and boletes. Tokai University Press, Tokyo, 303 pp.
Tolgor, B., Bao, H.Y. & Li, Y. (2014) A revised checklist of poisonous mushrooms in China. Mycosystema 33 (3): 517–548. https://doi.org/10.1080/15572536.2006.11832626
Vadthanarat, S., Lumyong, S. & Raspé, O. (2019) Cacaoporus, a new Boletaceae genus, with two new species from Thailand. MycoKeys 54: 1–29. https://dx.doi.org/10.3897%2Fmycokeys.54.35018
Vadthanarat, S., Raspé, O. & Lumyong, S. (2018) Phylogenetic affinities of the sequestrate genus Rhodactina (Boletaceae), with a new species, R. rostratispora from Thailand. MycoKeys 29: 63–80. https://dx.doi.org/10.3897%2Fmycokeys.29.22572
Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.199
Watling, R. (2008) A manual and source book of the boletes and their allies. Synopsis Fungorum, vol 24. Fungiflora, Oslo, 250 pp.
Watling, R. & Turnbull, E. (1994) Boletes from South & East Central Africa - II. Edinburgh Journal of Botany 51: 331–353.
White, T.J., Bruns, T., Lee, S. & Taylor, J.W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenies. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR protocols: a guide to methods and applications. Academic Press, New York, pp. 315–322.
Wu, G., Feng, B., Xu, J., Zhu, X.T., Li, Y.C., Zeng, N.K., Hosen, M.I. & Yang, Z.L. (2014) Molecular phylogenetic analyses redefine seven major clades and reveal 22 new generic clades in the fungal family Boletaceae. Fungal Diversity 69: 93–115. https://dx.doi.org/10.1007/s13225-014-0283-8
Wu, G., Li, Y.C., Zhu, X.T., Zhao, K., Han, L.H., Cui, Y.Y., Li, F., Xu, J.P. & Yang, Z.L. (2016) One hundred noteworthy boletes from China. Fungal Diversity 81: 25–188. https://doi.org/10.1007/s13225-016-0375-8
Wu, L.L., Liang, Z.Q., Su, M.S., Fan, Y.G., Zhang, P., Jiang, S., Chen, L.Y., Hao, J.Y. & Zeng, N.K. (2021) Updated taxonomy of Chinese Phylloporus (Boletaceae, Boletales): six new taxa and four redescribed species. Mycological Progress 20: 1243–1273. https://doi.org/10.1007/s11557-021-01722-8
Zeng, N.K., Tang, L.P., Li, C.Y., Tolgor, B., Zhu, X.T., Zhao, Q. & Yang, Z.L. (2013) The genus Phylloporus (Boletaceae, Boletales) from China: morphological and multilocus DNA sequence analyses. Fungal Diversity 58: 73–101. https://dx.doi.org/10.1007/s13225-012-0184-7
Zeng, N.K. & Jiang, S. (2020) Atlas of macrofungi from Yinggeling of Hainan, China. Nanhai Publishing House, Hainan.
Zhang, R., Xing, F., Siu, L., Ye, Y., Ng, S., Chen, H. & Wang, F. (2007) Spermatophyte flora of Yinggeling Mountain, Hainan. Biodiversity Science 15: 382–392. https://doi.org/10.1360/biodiv.060278
Zhao, K., Zhang, F.M., Zeng, Q.Q., Han, L.H. & Li, Y.C. (2020) Tylopilus jiangxiensis, a new species of Tylopilus s. str. from China. Phytotaxa 434: 281–291. https://doi.org/10.11646/phytotaxa.434.3.6
Bau, T., Bao, H.Y. & Li, Y. (2014) A revised checklist of poisonous mushrooms in China. Mycosystema 33 (3): 517–548. https://doi.org/10.13346/j.mycosystema.130256
Binder, M. & Besl, H. (2000) 28S rDNA sequence data and chemotaxonomical analyses on the generic concept of Leccinum (Boletales). Mycologia 75–86.
Binder, M. & Hibbett, D.S. (2006) Molecular systematics and biological diversification of Boletales. Mycologia 98: 971–981. https://doi.org/10.1080/15572536.2006.11832626
Bulliard, J.B.F. (1788) Herbier de la France 8. Privately published, Paris. pp. 337–384.
Castresana, J. (2000) Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Molecular Biology and Evolution 17: 540–552. https://doi.org/10.1093/oxfordjournals.molbev.a026334
Chai, H., Liang, Z.Q., Xue, R., Jiang, S., Luo, S.H., Wang, Y., Wu, L.L., Tang, L.P., Chen, Y., Hong, D. & Zeng, N.K. (2019) New and noteworthy boletes from subtropical and tropical China. Mycokeys 46: 55–96. https://doi.org/10.3897/mycokeys.46.31740
Chakraborty, D., Vizzini, A. & Das, K. (2018) Two new species and one new record of the genus Tylopilus (Boletaceae) from Indian Himalaya with morphological details and phylogenetic estimations. MycoKeys 33: 103–124. https://dx.doi.org/10.3897%2Fmycokeys.33.23703
Corner, E.J.H. (1972) Boletus in Malaysia. Government Printer, Singapore, 263 pp.
Corrales, A., Turner, B.L., Tedersoo, L., Anslan, S. & Dalling, J.M. (2017) Nitrogen addition alters ectomycorrhizal fungal communities and soil enzyme activities in a tropical montane forest. Fungal Ecology 27: 14–23. https://doi.org/10.1016/j.funeco.2017.02.00
Dentinger, B.T., Ammirati, J.F., Both, E.E., Desjardin, D.E., Halling, R.E., Henkel, T.W., Moreau, P.A., Nagasawa, E., Soytong, K., Taylor, A.F., Watling, R., Moncalvo, J.M. & McLaughlin, D.J. (2010) Molecular phylogenetics of porcini mushrooms (Boletus section Boletus). Molecular Phylogenetics and Evolution 57: 1276–1292. https://dx.doi.org/10.1016/j.ympev.2010.10.00
Edgar, R.C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32: 1792–1797. https://doi.org/10.1093/nar/gkh340
Fu, S.Z., Wang, Q.B. & Yao, Y.J. (2006) Tylopilus microsporus, a new species from Southwest China. Mycotaxon 96: 41–46.
Fulgenzi, T.D., Henkel, T.W. & Halling, R.E. (2007) Tylopilus orsonianus sp. nov. and Tylopilus eximius from Guyana. Mycologia 99: 622–627. https://dx.doi.org/10.1080/15572536.2007.11832556
Gelardi, M., Angelini, C., Costanzo, F., Dovana, F., Santana, B.O. & Vizzini, A. (2019) Tylopilus griseiolivaceus sp. nov. and T. leucomycelinus (Boletaceae) revisited from the Dominican Republic within a comprehensive phylogeny of Tylopilus s. str. Mycological Progress 18: 1039–1056.
Gelardi, M., Vizzini, A., Ercole, E., Taneyama, Y., Li, T.H., Zhang, M., Yan, W.J. & Wang, W.J. (2015) New collection, iconography and molecular evidence for Tylopilus neofelleus (Boletaceae, Boletoideae) from southwestern China and the taxonomic status of T. plumbeoviolaceoides and T. microsporus. Mycoscience 56: 373–386. https://dx.doi.org/10.1016/j.myc.2014.12.001
Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98. http://doi.org/10.14601/Phytopathol_Mediterr-14998u1.29
Halling, R.E., Nuhn, M., Osmundson, T., Fechner, N., Trappe, J.M., Soytong, K., Arora, D., Hibbett, D.S. & Binder, M. (2012) Affinities of the Boletus chromapes group to Royoungia and the description of two new genera, Harrya and Australopilus. Australian Systematic Botany 25: 418–431. https://doi.org/10.1071/SB12028
Halling, R.E., Osmundson, T.W. & Neves, M.A. (2008) Pacific boletes: implications for biogeographic relationships. Mycological Research 112: 437–447. https://doi.org/10.1016/j.mycres.2007.11.021
Henkel, T.W. (1999) New taxa and distribution records for Tylopilus from Dicymbe forests of Guyana. Mycologia 91: 655–665. https://dx.doi.org/10.2307/3761252
Hongo, T. (1964) Notulae mycologicae (3). Memoirs of Faculty Education of Shiga University, Natural Sciences 14: 43–47.
Hongo, T. (1966) Notulae mycologicae (5). Memoirs of the Faculty of Education of Shiga University, Natural Sciences 16: 57–62.
Hongo, T. (1967) Notes on Japanese larger fungi (19). Journal of Japanese Botany 42: 151–159.
Horak, E. (2011) Revision of Malaysian Species of Boletales s.l. (Basidiomycota) Described by E.J.H. Corner (1972, 1974). Malayan Forest Records 51: 1–283.
Huelsenbeck, J.P. & Ronquist, F. (2005) Bayesian analysis of molecular evolution using MrBayes. In: Nielsen, R. (Ed.) Statistical methods in molecular evolution. Springer, New York, pp. 183–226. https://doi.org/10.1007/0-387-27733-1_7
James, T.Y., Kauff, F., Schoch, C., Matheny, P.B., Hofstetter, V., Cox, C., Celio, G., Gueidan, C., Fraker, E., Miadlikowska, J., Lumbsch, H.T., Rauhut, A., Reeb, V., Arnold, A.E., Amtoft, A., Stajich, J.E., Hosaka, K., Sung, G.H., Johnson, D., O’Rourke, B., Crockett, M., Binder, M., Curtis, J.M., Slot, J.C., Wang, Z., Wilson, A.W., Schüßler, A., Longcore, J.E., O’Donnell, K., Mozley-Standridge, S., Porter, D., Letcher, P.M., Powell, M.J., Taylor, J.W., White, M.M., Griffith, G.W., Davies, D.R., Humber, R.A., Morton, J.B., Sugiyama, J., Rossman, A.Y., Rogers, J.D., Pfister, D.H., Hewitt, D., Hansen, K., Hambleton, S., Shoemaker, R.A., Kohlmeyer, J., Volkmann-Kohlmeyer, B., Spotts, R.A., Serdani, M., Crous, P.W., Hughes, K.W., Matsuura, K., Langer, E., Langer, G., Untereiner, W.A., Lücking, R., Büdel, B., Geiser, D.M., Aptroot, A., Diederich, P., Schmitt, I., Schultz, M., Yahr, R., Hibbett, D., Lutzoni, F., McLaughlin, D., Spatafora, J. & Vilgalys, R. (2006) Reconstructing the early evolution of the fungi using a six gene phylogeny. Nature 443: 818–822. http://dx.doi.org/10.1038/nature05110
Jiang, H.S., Chen, B.L., Zhou, Y.D., Wang, C.D., Fang, L. & Luo, Y.K. (2013) Biodiversity and conservation of Hainan Yinggeling Nature Reserve. China Forestry Press, Beijing, 629 pp.
Karsten, P.A. (1881) Enumeratio boletinearum et polyporearum fennicarum, systemate novo dispositarum. Revue Mycologique 3: 16–19.
Kornerup, A. & Wanscher, J.H. (1981) Taschenlexikon der Farben. 3. Aufl. Muster-Schmidt Verlag, Göttingen, 242 pp.
Kuntze, O. (1898) Revisio generum plantarum 3. Arthur Felix, Dulau & Co., Leipzig, 576 pp.
Lee, S.J., Yun, B.S., Cho, D.H. & Yoo, I.D. (1999) Tylopeptins A and B, new antibiotic peptides from Tylopilus neofelleus. Journal of Bacteriology 52: 998–1006. https://doi.org/10.7164/antibiotics.52.998
Li, T.H., Song, B. & Shen, Y.H. (2002) A new species of Tylopilus from Guangdong. Mycosystema 21: 3–5.
Li, Y.C., Feng, B. & Yang, Z.L. (2011) Zangia, a new genus of Boletaceae supported by molecular and morphological evidence. Fungal Diversity 49: 125–143.
Li, Y.C. & Yang, Z.L. (2021) The boletes of China: Tylopilus s.l. Springer, Berlin, 418 pp. https://doi.org/10.1007/978-981-16-2986-0
Liang, Z.Q., Su, M.S., Jiang, S., Hong, D. & Zeng, N.K. (2018) Tylopilus callainus, a new species with a sea-green color change of hymenophore and context from the south of China. Phytotaxa 343: 269–276. https://doi.org/10.11646/phytotaxa.343.3.7
Magnago, A.C., Reck, M.A., Dentinger, B.T.M., Moncalvo, J.M., Neves, M.A. & Silveira, R.M.B.D. (2017) Two new Tylopilus species (Boletaceae) from Northeastern Atlantic Forest, Brazil. Phytotaxa 316: 250–260. https://dx.doi.org/10.11646/phytotaxa.316.3.4
Mazzer, S.J. & Smith, A.H. (1967) New and interesting boletes from Michigan. The Michigan Botanist 6: 57–67.
Nagy, L.G., Kocsubé, S., Csanádi, Z., Kovács, G.M., Petkovits, T., Vágvölgyi, C. & Papp, T. (2012) Re-mind the gap! Insertion–deletion data reveal neglected phylogenetic potential of the nuclear ribosomal internal transcribed spacer (ITS) of fungi. PLoS ONE 7: e49794. https://doi.org/10.1371/journal.pone.0049794
Nuhn, M.E., Binder, M., Taylor, A.F.S., Halling, R.E. & Hibbett, D.S. (2013) Phylogenetic overview of the Boletineae. Fungal Biology 117: 479–511. https://doi.org/10.1016/j.funbio.2013.04.008
Nylander, J.A.A. (2004) MrModeltest 2.3. program distributed by the author. Evolutionary Biology Center, Uppsala University.
Osmundson, T. & Halling, R.E. (2010) Tylopilus oradivensis sp. nov.: a newly described member of the Tylopilus balloui complex from Costa Rica. Mycotaxon 113: 475–483. https://doi.org/10.5248/113.475
Osmundson, T.W., Bougher, N.L., Robinson, R.M. & Halling, R.E. (2021) Rubinoboletus phaseolisporus (Boletaceae) from Western Australia is a Tylopilus with bean-shaped spores. Nuytsia 32: 87–97.
Pegler, D.N. & Young, T.W.K. (1981) A natural arrangement of the Boletales, with reference to spore morphology. Transactions of the British Mycological Society 76: 103–146. https://doi.org/10.1016/S0007-1536(81)80013-7
Singer, R. (1947) The Boletoideae of Florida. The Boletineae of Florida with notes on extralimital species III. The American Midland Naturalist 37: 1–135.
Singer, R. (1986) The Agaricales in modern taxonomy, 4th edn. Koeltz Scientific Books, Koenigstein, 981 pp.
Smith, S.A. & Dunn, C.W. (2008) Phyutility: a phyloinformatics tool for trees, alignments and molecular data. Bioinformation 24: 715–716. https://doi.org/10.1093/bioinformatics/btm619
Smith, M.E., Henkel, T.W., Aime, M.C., Fremier, A.K. & Vilgalys, R. (2011) Ectomycorrhizal fungal diversity and community structure on three co-occurring leguminous canopy tree species in a neotropical rainforest. New Phytologist 192: 699–712. https://doi.org/10.1111/j.1469-8137.2011.03844.
Stamatakis, A. (2006) RAxML-VI-HPC: maximum likelihood based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22: 2688–2690. https://doi.org/10.1093/bioinformatics/btl446
Terashima, Y., Takahashi, H. & Taneyama, Y. (2016) The fungal flora in Southwestern Japan: agarics and boletes. Tokai University Press, Tokyo, 303 pp.
Tolgor, B., Bao, H.Y. & Li, Y. (2014) A revised checklist of poisonous mushrooms in China. Mycosystema 33 (3): 517–548. https://doi.org/10.1080/15572536.2006.11832626
Vadthanarat, S., Lumyong, S. & Raspé, O. (2019) Cacaoporus, a new Boletaceae genus, with two new species from Thailand. MycoKeys 54: 1–29. https://dx.doi.org/10.3897%2Fmycokeys.54.35018
Vadthanarat, S., Raspé, O. & Lumyong, S. (2018) Phylogenetic affinities of the sequestrate genus Rhodactina (Boletaceae), with a new species, R. rostratispora from Thailand. MycoKeys 29: 63–80. https://dx.doi.org/10.3897%2Fmycokeys.29.22572
Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.199
Watling, R. (2008) A manual and source book of the boletes and their allies. Synopsis Fungorum, vol 24. Fungiflora, Oslo, 250 pp.
Watling, R. & Turnbull, E. (1994) Boletes from South & East Central Africa - II. Edinburgh Journal of Botany 51: 331–353.
White, T.J., Bruns, T., Lee, S. & Taylor, J.W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenies. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR protocols: a guide to methods and applications. Academic Press, New York, pp. 315–322.
Wu, G., Feng, B., Xu, J., Zhu, X.T., Li, Y.C., Zeng, N.K., Hosen, M.I. & Yang, Z.L. (2014) Molecular phylogenetic analyses redefine seven major clades and reveal 22 new generic clades in the fungal family Boletaceae. Fungal Diversity 69: 93–115. https://dx.doi.org/10.1007/s13225-014-0283-8
Wu, G., Li, Y.C., Zhu, X.T., Zhao, K., Han, L.H., Cui, Y.Y., Li, F., Xu, J.P. & Yang, Z.L. (2016) One hundred noteworthy boletes from China. Fungal Diversity 81: 25–188. https://doi.org/10.1007/s13225-016-0375-8
Wu, L.L., Liang, Z.Q., Su, M.S., Fan, Y.G., Zhang, P., Jiang, S., Chen, L.Y., Hao, J.Y. & Zeng, N.K. (2021) Updated taxonomy of Chinese Phylloporus (Boletaceae, Boletales): six new taxa and four redescribed species. Mycological Progress 20: 1243–1273. https://doi.org/10.1007/s11557-021-01722-8
Zeng, N.K., Tang, L.P., Li, C.Y., Tolgor, B., Zhu, X.T., Zhao, Q. & Yang, Z.L. (2013) The genus Phylloporus (Boletaceae, Boletales) from China: morphological and multilocus DNA sequence analyses. Fungal Diversity 58: 73–101. https://dx.doi.org/10.1007/s13225-012-0184-7
Zeng, N.K. & Jiang, S. (2020) Atlas of macrofungi from Yinggeling of Hainan, China. Nanhai Publishing House, Hainan.
Zhang, R., Xing, F., Siu, L., Ye, Y., Ng, S., Chen, H. & Wang, F. (2007) Spermatophyte flora of Yinggeling Mountain, Hainan. Biodiversity Science 15: 382–392. https://doi.org/10.1360/biodiv.060278
Zhao, K., Zhang, F.M., Zeng, Q.Q., Han, L.H. & Li, Y.C. (2020) Tylopilus jiangxiensis, a new species of Tylopilus s. str. from China. Phytotaxa 434: 281–291. https://doi.org/10.11646/phytotaxa.434.3.6