Abstract
Volvariella clavocystidiata sp. nov., collected from Western Siberia, is described based on morphological characters and molecular data. The new species is characterized by silky fibrillose whitish pileus, clavate cheilo- and pleurocystidia, and basidiospores on average 6.6 × 4.8 µm and swamp habitat. Detailed morphological descriptions, drawings of microstructures and comparisons with closely related taxa are provided. Phylogenetic analyses inferred from the internal transcribed spacer (nrITS) and 28S nuclear ribosomal large subunit (nrLSU) showed that novel taxon formed independent phylogenetic lineage within the genus Volvariella.
References
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Fries, E.M. (1849) Summa vegetabilium Scandinaviae. II. Typographica Academica, Uppsala, pp. 259–572.
Gardes, M. & Bruns, T.D. (1993) ITS primers with enhanced specificity for basidiomycetes application to the identification of mycorrhizae and rusts. Molecular Ecology 2: 132–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
Grigoriev, I.V., Nikitin, R., Haridas, S., Kuo, A., Ohm, R., Otillar, R., Riley, R., Salamov, A., Zhao, X., Korzeniewski, F., Smirnova, T., Nordberg, H., Dubchak, I., Shabalov, I. (2014) MycoCosm portal: gearing up for 1000 fungal genomes. Nucleic Acids Research 42:D699–D704. https://doi.org/10.1093/nar/gkt1183
Justo, A., Vizzini, A., Minnis, A.M., Menolli, N.Jr., Capelari, M., Rodríguez, O., Malysheva, E., Contu, M., Chignone, S. & Hibbett, D.S. (2011a) Phylogeny of the Pluteaceae (Agaricales, Basidiomycota): taxonomy and character evolution. Fungal Biology 115 (1): 1–20. https://doi.org/10.1016/j.funbio.2010.09.012
Justo, A., Minnis, A.M., Ghignone, S., Menolli, N.Jr., Capelari, M., Rodríguez, O., Malysheva, E., Contu, M. & Vizzini, A. (2011b) Species recognition in Pluteus and Volvopluteus (Pluteaceae, Agaricales): morphology, geography and phylogeny. Mycological Progress 10 (4): 453–479. https://doi.org/10.1007/s11557-010-0716-z
Kotlaba, F. & Pouzar, Z. (1972) Taxonomic and nomenclatural notes on some Macromycetes. ?eská Mykologie 26 (4): 217–222.
Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. (2018) MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution 35: 1547–1549. https://doi.org/10.1093/molbev/msy096
Kummer, P. (1871) Der Führer in die Pilzkunde. Edn. 1. E. Luppe, Zerbst, 146 pp.
Malysheva, E., Malysheva, V., Alexandrova, A. & Morozova, O. (2019) Observations on Pluteaceae in Vietnam. 1. New species and new records of Volvariella. Phytotaxa 408 (4): 233–254. https://doi.org/10.11646/phytotaxa.408.4.1
Matheny, P.B., Curtis, J.M., Hofstetter, V., Aime, M.C., Moncalvo, J-M., Ge, Z.-W., Yang, Z.-L., Slot, J.C., Ammirati, J.F., Baroni, T.J., Bougher, N.L., Hughes, K.W., Lodge, D.J., Kerrigan, R.W., Seidl, M.T., Aanen, D.K., DeNitis, M., Daniele, G.M., Desjardin, D.E., Kropp, B.R., Norvell, L.L., Parker, A., Vellinga, E.C., Vilgalys, R. & Hibbett, D.S. (2006) Major clades of Agaricales: a multilocus phylogenetic overview. Mycologia 98: 982–995.
Moncalvo, J.M., Vilgalys, R., Redhead, S.A., Johnson, J.E., James, T.Y., Aime, M.C., Hofstetter, V., Verduin, S.J.W., Larsson, E., Baroni, T.J., Thorn, R.G., Jacobsson, S., Clémençon, H. & Miller, Jr. O.K. (2002) One hundred and seventeen clades of euagarics. Molecular Phylogenetics and Evolution 23: 357e400. https://doi.org/10.1016/S1055-7903(02)00027-1
Orton, P.D. (1986) Pluteaceae: Pluteus & Volvariella. In: Henderson, D.M., Orton, P.D. & Watling, R. (Eds.) British Fungus Flora: Agarics and Boleti, vol. 4. Edinburgh, Royal Botanic Garden, 157 pp.
Rambaut, A., Drummond, A.J., Xie, D., Baele, G. & Suchard, M.A. (2018) Posterior summarisation in Bayesian phylogenetics using Tracer 1.7. Systematic Biology 67 (5): 901–904. https://doi.org/10.1093/sysbio/syy032
Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Hohna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61: 539–542. https:// doi.org/10.1093/sysbio/sys029. PMID:22357727.
Shaffer, R.L. (1957) Volvariella in North America. Mycologia 49 (4): 545–579.
Spegazzini, C. (1898) Fungi Argentini novi vel critici. Anales del Museo Nacional de Historia Natural Buenos Aires 6: 81–288.
Varga, T., Krizsan, K., Foldi, C., Dima, B., Sanchez-Garcia, M., Sanchez-Ramirez, S., Szollosi, G.J., Szarkandi, J.G., Papp, V., Albert, L., Andreopoulos, W., Angelini, C., Antonin, V., Barry, K.W., Bougher, N.L., Buchanan, P., Buyck, B., Bense, V., Catcheside, P., Chovatia, M., Cooper, J., Damon, W., Desjardin, D., Finy, P., Geml, J., Haridas, S., Hughes, K., Justo, A., Karasinski, D., Kautmanova, I., Kiss, B., Kocsube, S., Kotiranta, H., LaButti, K.M., Lechner, B.E., Liimatainen, K., Lipzen, A., Lukacs, Z., Mihaltcheva, S., Morgado, L.N., Niskanen, T., Noordeloos, M.E., Ohm, R.A., Ortiz-Santana, B., Ovrebo, C., Racz, N., Riley, R., Savchenko, A., Shiryaev, A., Soop, K., Spirin, V., Szebenyi, C., Tomsovsky, M., Tulloss, R.E., Uehling, J., Grigoriev, I.V., Vagvolgyi, C., Papp, T., Martin, F.M., Miettinen, O., Hibbett, D.S., Nagy, L.G. (2019) Megaphylogeny resolves global patterns of mushroom evolution. Nature Ecology and Evolution 3: 668–678. https:// doi.org/10.1038/s41559-019-0834-1
Vellinga, E.C. (1988) Glossary. In: Bas, C., Kuyper, Th.W., Noordeloos, M.E. & Vellinga, E.C. (Eds.) Flora Agaricina Neerlandica, vol. 1. A.A. Balkema, Rotterdam, pp. 54–64.
Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4239–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
Vizzini, A., Contu, M. & Justo, A. (2011) Additional records of Volvariella dunensis (Basidiomycota, Agaricales): morphological and molecular characterization. Mycotaxon 117: 37–43. https://doi.org/10.5248/117.37
White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR Protocols: a guide to methods and applications. Academic Press, New York, pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
Bigelow, H.E. & Smith, A.H. (1973) Cantharocybe, a new genus of Agaricales. Mycologia 65 (2): 485–488. https://doi.org/10.1080/00275514.1973.12019458
Boekhout, T. (1990) Volvariella. In: Bas, C., Kuyper, Th.W., Noordeloos, M.E. & Vellinga, E.C. (Eds.) Flora Agaricina Neerlandica 2. A.A. Balkema, Rotterdam, pp. 56–64.
Clémençon, H. (2009) Methods for working with macrofungi. Laboratory cultivation and preparation of large fungi for light microscopy. IHW-Verlag, 88 pp.
Crous, P.W., Wingfield, M.J., Chooi, Y.H., Gilchrist, C.L.M., Lacey, E., Pitt, J.I., Roets, F., Swart, W.J., Cano-Lira, J.F., Valenzuela-Lopez, N., Hubka, V., Shivas, R.G., Stchigel, A.M., Holdom, D.G., Jurjevi?, Ž., Kachalkin, A.V., Lebel, T., Lock, C., Martín, M.P., Tan, Y.P., Tomashevskaya, M.A., Vitelli, J.S., Baseia, I.G., Bhatt, V.K., Brandrud, T.E., De Souza, J.T., Dima, B., Lacey, H.J., Lombard, L., Johnston, P.R., Morte, A., Papp, V., Rodríguez, A., Rodríguez-Andrade, E., Semwal, K.C., Tegart, L., Abad, Z.G., Akulov, A., Alvarado, P., Alves, A., Andrade, J.P., Arenas, F., Asenjo, C., Ballarà, J., Barrett, M.D., Berná, L.M., Berraf-Tebbal, A., Bianchinotti, M.V., Bransgrove, K., Burgess, T.I., Carmo, F.S., Chávez, R., ?moková, A., Dearnaley, J.D.W., Santiago, A.L.C.M. de A., Freitas-Neto, J.F., Denman, S., Douglas, B., Dovana, F., Eichmeier, A., Esteve-Raventós, F., Farid, A., Fedosova, A.G., Ferisin, G., Ferreira, R.J., Ferrer, A., Figueiredo, C.N., Figueiredo, Y.F., Reinoso-Fuentealba, C.G., Garrido-Benavent, I., Cañete-Gibas, C.F., Gil-Durán, C., Glushakova, A.M., Gonçalves, M.F.M., González, M., Gorczak, M., Gorton, C., Guard, F.E., Guarnizo, A.L., Guarro, J., Gutiérrez, M., Hamal, P., Hien, L.T., Hocking, A.D., Houbraken, J., Hunter, G.C., Inácio, C.A., Jourdan, M., Kapitonov, V.I., Kelly, L., Khanh, T.N., Kis?o, K., Kiss, L., Kiyashko, A., Kola?ík, M., Kruse, J., Kubátová, A., Ku?era, V., Ku?erová, I., Kušan, I., Lee, H.B., Levicán, G., Lewis, A., Liem, N.V., Liimatainen, K., Lim, H.J., Lyons, M.N., Maciá-Vicente, J.G., Magaña-Dueñas, V., Mahiques, R., Malysheva, E.F., Marbach, P.A.S., Marinho, P., Mato?ec, N., McTaggart, A.R., Meši?, A., Morin, L., Muñoz-Mohedano, J.M., Navarro-Ródenas, A., Nicolli, C.P., Oliveira, R.L., Otsing, E., Ovrebo, C.L., Pankratov, T.A., Paños, A., Paz-Conde, A., Pérez-Sierra, A., Phosri, C., Pintos, Á., Pošta, A., Prencipe, S., Rubio, E., Saitta, A., Sales, L.S., Sanhueza, L., Shuttleworth, L.A., Smith, J., Smith, M.E., Spadaro, D., Spetik, M., Sochor, M., Sochorová, Z., Sousa, J.O., Suwannasai, N., Tedersoo, L., Thanh, H.M., Thao, L.D., Tkal?ec, Z., Vaghefi, N., Venzhik, A.S., Verbeken, A., Vizzini, A., Voyron, S., Wainhouse, M., Whalley, A.J.S., Wrzosek, M., Zapata, M., Zeil-Rolfe, I. & Groenewald, J.Z. (2020) Fungal Planet description sheets: 1042–1111. Persoonia 44: 301–459. https://doi.org/10.3767/persoonia.2020.44.11
Fries, E.M. (1849) Summa vegetabilium Scandinaviae. II. Typographica Academica, Uppsala, pp. 259–572.
Gardes, M. & Bruns, T.D. (1993) ITS primers with enhanced specificity for basidiomycetes application to the identification of mycorrhizae and rusts. Molecular Ecology 2: 132–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
Grigoriev, I.V., Nikitin, R., Haridas, S., Kuo, A., Ohm, R., Otillar, R., Riley, R., Salamov, A., Zhao, X., Korzeniewski, F., Smirnova, T., Nordberg, H., Dubchak, I., Shabalov, I. (2014) MycoCosm portal: gearing up for 1000 fungal genomes. Nucleic Acids Research 42:D699–D704. https://doi.org/10.1093/nar/gkt1183
Justo, A., Vizzini, A., Minnis, A.M., Menolli, N.Jr., Capelari, M., Rodríguez, O., Malysheva, E., Contu, M., Chignone, S. & Hibbett, D.S. (2011a) Phylogeny of the Pluteaceae (Agaricales, Basidiomycota): taxonomy and character evolution. Fungal Biology 115 (1): 1–20. https://doi.org/10.1016/j.funbio.2010.09.012
Justo, A., Minnis, A.M., Ghignone, S., Menolli, N.Jr., Capelari, M., Rodríguez, O., Malysheva, E., Contu, M. & Vizzini, A. (2011b) Species recognition in Pluteus and Volvopluteus (Pluteaceae, Agaricales): morphology, geography and phylogeny. Mycological Progress 10 (4): 453–479. https://doi.org/10.1007/s11557-010-0716-z
Kotlaba, F. & Pouzar, Z. (1972) Taxonomic and nomenclatural notes on some Macromycetes. ?eská Mykologie 26 (4): 217–222.
Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. (2018) MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution 35: 1547–1549. https://doi.org/10.1093/molbev/msy096
Kummer, P. (1871) Der Führer in die Pilzkunde. Edn. 1. E. Luppe, Zerbst, 146 pp.
Malysheva, E., Malysheva, V., Alexandrova, A. & Morozova, O. (2019) Observations on Pluteaceae in Vietnam. 1. New species and new records of Volvariella. Phytotaxa 408 (4): 233–254. https://doi.org/10.11646/phytotaxa.408.4.1
Matheny, P.B., Curtis, J.M., Hofstetter, V., Aime, M.C., Moncalvo, J-M., Ge, Z.-W., Yang, Z.-L., Slot, J.C., Ammirati, J.F., Baroni, T.J., Bougher, N.L., Hughes, K.W., Lodge, D.J., Kerrigan, R.W., Seidl, M.T., Aanen, D.K., DeNitis, M., Daniele, G.M., Desjardin, D.E., Kropp, B.R., Norvell, L.L., Parker, A., Vellinga, E.C., Vilgalys, R. & Hibbett, D.S. (2006) Major clades of Agaricales: a multilocus phylogenetic overview. Mycologia 98: 982–995.
Moncalvo, J.M., Vilgalys, R., Redhead, S.A., Johnson, J.E., James, T.Y., Aime, M.C., Hofstetter, V., Verduin, S.J.W., Larsson, E., Baroni, T.J., Thorn, R.G., Jacobsson, S., Clémençon, H. & Miller, Jr. O.K. (2002) One hundred and seventeen clades of euagarics. Molecular Phylogenetics and Evolution 23: 357e400. https://doi.org/10.1016/S1055-7903(02)00027-1
Orton, P.D. (1986) Pluteaceae: Pluteus & Volvariella. In: Henderson, D.M., Orton, P.D. & Watling, R. (Eds.) British Fungus Flora: Agarics and Boleti, vol. 4. Edinburgh, Royal Botanic Garden, 157 pp.
Rambaut, A., Drummond, A.J., Xie, D., Baele, G. & Suchard, M.A. (2018) Posterior summarisation in Bayesian phylogenetics using Tracer 1.7. Systematic Biology 67 (5): 901–904. https://doi.org/10.1093/sysbio/syy032
Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Hohna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61: 539–542. https:// doi.org/10.1093/sysbio/sys029. PMID:22357727.
Shaffer, R.L. (1957) Volvariella in North America. Mycologia 49 (4): 545–579.
Spegazzini, C. (1898) Fungi Argentini novi vel critici. Anales del Museo Nacional de Historia Natural Buenos Aires 6: 81–288.
Varga, T., Krizsan, K., Foldi, C., Dima, B., Sanchez-Garcia, M., Sanchez-Ramirez, S., Szollosi, G.J., Szarkandi, J.G., Papp, V., Albert, L., Andreopoulos, W., Angelini, C., Antonin, V., Barry, K.W., Bougher, N.L., Buchanan, P., Buyck, B., Bense, V., Catcheside, P., Chovatia, M., Cooper, J., Damon, W., Desjardin, D., Finy, P., Geml, J., Haridas, S., Hughes, K., Justo, A., Karasinski, D., Kautmanova, I., Kiss, B., Kocsube, S., Kotiranta, H., LaButti, K.M., Lechner, B.E., Liimatainen, K., Lipzen, A., Lukacs, Z., Mihaltcheva, S., Morgado, L.N., Niskanen, T., Noordeloos, M.E., Ohm, R.A., Ortiz-Santana, B., Ovrebo, C., Racz, N., Riley, R., Savchenko, A., Shiryaev, A., Soop, K., Spirin, V., Szebenyi, C., Tomsovsky, M., Tulloss, R.E., Uehling, J., Grigoriev, I.V., Vagvolgyi, C., Papp, T., Martin, F.M., Miettinen, O., Hibbett, D.S., Nagy, L.G. (2019) Megaphylogeny resolves global patterns of mushroom evolution. Nature Ecology and Evolution 3: 668–678. https:// doi.org/10.1038/s41559-019-0834-1
Vellinga, E.C. (1988) Glossary. In: Bas, C., Kuyper, Th.W., Noordeloos, M.E. & Vellinga, E.C. (Eds.) Flora Agaricina Neerlandica, vol. 1. A.A. Balkema, Rotterdam, pp. 54–64.
Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4239–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
Vizzini, A., Contu, M. & Justo, A. (2011) Additional records of Volvariella dunensis (Basidiomycota, Agaricales): morphological and molecular characterization. Mycotaxon 117: 37–43. https://doi.org/10.5248/117.37
White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR Protocols: a guide to methods and applications. Academic Press, New York, pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1