Abstract
A new species, Leucoagaricus cupresseoides, from a pine forest in Castelfusano, Rome, Italy, is described based on both morphological and molecular analyses. Updated morphological descriptions of Leucoagaricus aurantiovergens and L. pseudopilatianus are presented due to new collections from Italy and confirmed by nrITS sequence analysis of the type specimens of the two species. Based on our morphological and molecular analyses, L. pseudopilatianus var. roseodiffractus and L. paracupresseus are synonyms of L. aurantiovergens; and L. pseudopilatianus var. rugosoreticulatus is not molecularly distinct from L. pseudopilatianus. Leucoagaricus cupresseus, L. cupresseoides, L. aurantiovergens and L. pseudopilatianus form the L. cupresseus complex for which a comparative table is provided.
References
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Priou, J.P., Bodin, M. & Guinberteau, J. (1995) Une espèce méconnue: Leucoagaricus gaillardii Bon & Boiffard – nouvelles données morphologiques, écologiques, chorologiques. Documents mycologiques 25: 349–358.
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Singer, R. (1973) Diagnoses fungorum novorum Agaricalium III. Beihefte zur Sydowia 7: 1–106.
Ullah, Z., Jabeen, S., Faisal, M., Ahmad, H. & Khalid, A.N. (2019) Leucoagaricus brunneus sp. nov. from Khyber Pakhtunkhwa, Pakistan. Mycotaxon 134: 601–611. https://doi.org/10.5248/134.601
Vellinga, E.C. (1988) Glossary. In: Bas, C., Kuyper, T.W., Noordeloos, M.E. & Vellinga, E.C. (Eds.) Flora Agaricina Neerlandica. Critical monographs on families of agarics and boleti occurring in the Netherlands, vol 1. A.A. Balkema, Rotterdam, pp. 54–64.
Vellinga, E.C. (2001) Leucoagaricus Locq. ex Singer. In: Noordeloos, M.E., Kuyper, T.W. & Vellinga, E.C. (Eds.) Flora Agaricina Neerlandica. Critical monographs on families of agarics and boleti occurring in the Netherlands, vol 5. A.A. Balkema, Lisse, pp. 85–108.
Vellinga, E.C. (2004) Genera in the family Agaricaceae: evidence from nrITS and nrLSU sequences. Mycological Research 108: 354–377. https://doi.org/10.1017/S0953756204009700
Vellinga, E.C. (2010) Lepiotaceous fungi in California, USA. Leucoagaricus sect. Piloselli. Mycotaxon 112: 393–444. https://doi.org/10.5248/112.393
Vellinga, E.C., Contu, M. & Vizzini, A. (2010) Leucoagaricus decipiens and La. erythrophaeus, a new species pair in sect. Piloselli. Mycologia 102: 447–454. https://doi.org/10.3852/09-164
Vellinga, E.C., Sysouphanthong, P. & Hyde, K.D. (2011) The family Agaricaceae: phylogenies and two new white-spored genera. Mycologia, 103: 494–509. https://doi.org/10.3852/10-204
Vizzini, A., Lezzi, T., Tatti, A., Iannootti, M., Filippa, M. & Dovana, F. (2017) Molecular confirmation of Leucoagaricus idae-fragum (Agaricales, Agaricaceae), and notes on its morphological variability. Phytotaxa 332: 157–171. https://doi.org/10.11646/phytotaxa.332.2.3
White, T.J., Bruns, T., Lee, S. & Taylor, J.W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR protocols: a guide to methods and applications. Academic Press, New York, pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
Asif, M., Niazi, A.R., Izhar, A., Khalid, A.N. & Bashir, H. (2021) Leucoagaricus fragilis sp. nov. (Agaricaceae) from Punjab, Pakistan. Phytotaxa 501: 140–150. https://doi.org/10.11646/phytotaxa.501.1.5
Boisselet, P. & Guinberteau, J. (2001) Leucoagaricus cupresseus (Burlingham) Boisselet & Guinberteau comb. nov., une lépiote cupressicole d’origine américaine récolltée en France. Bulletin de la Féderation des Associations mycologiques méditerranéennes, n.s. 19: 33–42.
Bon, M. & Boiffard, J. (1974) Lépiotes de Vendée et de la côte Atlantique française. Bulletin de la Société Mycologique de France 90: 287–306.
Bon, M. (1976) Novitates. Documents mycologiques 6: 41–46.
Bon, M. (1993) Flore mycologique d’Europe, 3. Les Lépiotes. Lepiotaceae Roze. Documents mycologiques, Mémoire hors série 3: 1–153.
Consiglio, G., Setti, L. & Vizzini, A. (2021) A new species of Leucoagaricus (Agaricaceae, Agaricales) found in Italy. Micologia e Vegetazione Mediterranea 36: 201–216.
Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature Methods 9: 772. https://doi.org/10.1038/nmeth.2109
Dovana, F., Contu, M., Angeli, P., Brandi, A. & Mucciarelli, M. (2017) Leucoagaricus ariminensis sp. nov., a lilac species from Italy. Mycotaxon 132: 205–216. https://doi.org/10.5248/132.205
Dutta, A.K., Stallman, J.K., Bera, S., Hoque, E., Paloi, S. & Acharya, K. (2021) Lepiotaceous fungi of West Bengal, India: two new species of Leucoagaricus. Mycological Progress 20: 493–507. https://doi.org/10.1007/s11557-021-01685-w
Forin, N., Nigris, S., Voyron, S., Girlanda, M., Vizzini, A., Casadoro, G. & Baldan, B. (2018) Next generation sequencing of ancient fungal specimens: the case of the Saccardo mycological herbarium. Frontiers in Ecology and Evolution 6: 129. https://doi.org/10.3389/fevo.2018.00129
Gardes, M. & Bruns, T.D. (1993) ITS primers with enhanced specificity for Basidiomycetes–application to the identification of mycorrhizae and rusts. Molecular Ecology 2: 113–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
Gliozzi, V. (1999) Una nuova entitaÌ della sezione Piloselli: Leucoagaricus aurantiovergens sp. nov. Rivista di Micologia 41: 291–300.
Gennari, A., Migliozzi, V. & Siquier Virgós, J.L. (1995) Tre interessanti Leucocoprinae sabulicole. Leucoagaricus gaillardii Bon & Boiffard, Sericeomyces subvolvatus (Malençon & Bertault) Contu fo. subvolvatus e S. subvolvatus fo. pictus (Malençon & Bertault) Bon. Micologia e Vegetazione Mediterranea 10: 9–21.
Justo, A., Angelini, C. & Bizzi, A. (2021) The genera Leucoagaricus and Leucocoprinus in the Dominican Republic. Mycologia 113: 348–389. https://doi.org/10.1080/00275514.2020.1819142
Katoh, K., Misawa, K., Kuma, K. & Miyata, T. (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Research 30: 3059–3066. https://doi.org/10.1093/nar/gkf436
Kozlov, A.M., Darriba, D., Flouri, T., Morel, B. & Stamatakis, A. (2019) RAxML-NG: A fast, scalable and user-friendly tool for max-imum likelihood phylogenetic inference. Bioinformatics 35: 4453–4455. https://doi.org/10.1093/bioinformatics/btz305
Latha, K.P.D., Raj, K.N.A. & Manimohan, P. (2020) Leucoagaricus callainitinctus—a new species of Leucoagaricus section Piloselli (Agaricaceae) from tropical India. Phytotaxa 442: 111–120. https://doi.org/10.11646/phytotaxa.442.2.6
Liang, J.F., Yang, Z.L., Xu, J. & Ge, Z.W. (2010) Two new unusual Leucoagaricus species (Agaricaceae) from tropical China with blue-green staining reactions. Mycologia 102: 1141–1152. https://doi.org/10.3852/09-021
Maddison, W.P. & Maddison, D.R. (2019) Mesquite: a modular system for evolutionary analysis. Available from: http://www.mesquiteproject.org (accessed 23 February 2022)
Migliozzi, V. & Perrone, L. (1992) Sulle Lepiotee - 8° contributo. Descrizione di Leucoagaricus brunnescens (Peck) Bon e creazione della sottosezione Pilatianei Migliozzi et Perrone. Bollettino dell’associazione micologica ed ecologica romana 26: 3–9.
Migliozzi, V. & Resta, G. (2001) Note sulla sottosezione Pilatiani del genere Leucoagaricus. Due nuove varietà: Leucoagaricus pseudopilatianus var. rugosoreticulatus e Leucoagaricus pseudopilatianus var. roseodiffractus. Micologia e Vegetazione Meditteranea 15: 129–156.
Migliozzi, V., Rocabruna, A. & Tabarés, M. (2001) Leucoagaricus pseudopilatianus, una nueva especie de la sección Piloselli. Revista Catalana de Micologia 23: 67–74.
Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: Proceedings of the Gateway Computing Environments Workshop (GCE). New Orleans, LA, pp. 1–8. https://doi.org/10.1109/GCE.2010.5676129
Muñoz, G., Caballero, A., Contu, M. & Vizzini, A. (2012) A new Leucoagaricus species of section Piloselli (Agaricales, Agaricaceae) from Spain. IMA Fungus 3: 117–123. https://doi.org/10.5598/imafungus.2012.03.02.03
Muñoz, G., Caballero, A., Contu, M., Ercole, E. & Vizzini, A. (2014) Leucoagaricus croceobasis (Agaricales, Agaricaceae), a new species of section Piloselli from Spain. Mycological Progress 13: 649–655. https://doi.org/10.1007/s11557-013-0947-x
Patouillard, N.T. (1888) Quelques points de la classification des Agaricinées. Journal de Botanique (Morot) 2: 12–16.
Priou, J.P., Bodin, M. & Guinberteau, J. (1995) Une espèce méconnue: Leucoagaricus gaillardii Bon & Boiffard – nouvelles données morphologiques, écologiques, chorologiques. Documents mycologiques 25: 349–358.
Redhead, S.A. (2016) Nomenclatural novelties. Index Fungorum 315: 1.
Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61: 539–542. https://doi.org/10.1093/sysbio/sys029
Saccardo, P.A. (1887) Sylloge Hymenomycetum, Vol. I. Agaricineae. Sylloge Fungorum 5: 1–1146.
Salom, L.C., Siquier, J.L., Planas, J. & Espinosa, J. (2021a) Leucoagaricus paracupresseus Salom, Siquier, Planas & Espinosa, sp. nov. Index Fungorum 485: 1.
Salom, L.C., Siquier, J.L., Planas, J. & Espinosa, J. (2021b) Contribuciòn al conoscimiento de Lepiota s.l. en las Illes Baleares (España) VII. Leucoagaricus paracupresseus Salom, Siquier, Planas & Espinosa. Erratori 18: 148–157.
Schindelin, J., Arganda-Carreras, I., Frise, E., Kaynig, V., Longair, M., Pietzsch, T., Preibisch, S., Rueden, C., Saalfeld, S., Schmid, B., Tinevez, J.-Y., White, D.J., Hartenstein, V., Eliceiri, K., Tomancak, P. & Cardona, A. (2012) Fiji: an open-source platform for biological-image analysis. Nature methods 9: 676–682. https://doi.org/10.1038/nmeth.2019
Singer, R. (1948) Diagnoses fungorum novarum Agaricalium. Sydowia 2: 26–42.
Singer, R. (1973) Diagnoses fungorum novorum Agaricalium III. Beihefte zur Sydowia 7: 1–106.
Ullah, Z., Jabeen, S., Faisal, M., Ahmad, H. & Khalid, A.N. (2019) Leucoagaricus brunneus sp. nov. from Khyber Pakhtunkhwa, Pakistan. Mycotaxon 134: 601–611. https://doi.org/10.5248/134.601
Vellinga, E.C. (1988) Glossary. In: Bas, C., Kuyper, T.W., Noordeloos, M.E. & Vellinga, E.C. (Eds.) Flora Agaricina Neerlandica. Critical monographs on families of agarics and boleti occurring in the Netherlands, vol 1. A.A. Balkema, Rotterdam, pp. 54–64.
Vellinga, E.C. (2001) Leucoagaricus Locq. ex Singer. In: Noordeloos, M.E., Kuyper, T.W. & Vellinga, E.C. (Eds.) Flora Agaricina Neerlandica. Critical monographs on families of agarics and boleti occurring in the Netherlands, vol 5. A.A. Balkema, Lisse, pp. 85–108.
Vellinga, E.C. (2004) Genera in the family Agaricaceae: evidence from nrITS and nrLSU sequences. Mycological Research 108: 354–377. https://doi.org/10.1017/S0953756204009700
Vellinga, E.C. (2010) Lepiotaceous fungi in California, USA. Leucoagaricus sect. Piloselli. Mycotaxon 112: 393–444. https://doi.org/10.5248/112.393
Vellinga, E.C., Contu, M. & Vizzini, A. (2010) Leucoagaricus decipiens and La. erythrophaeus, a new species pair in sect. Piloselli. Mycologia 102: 447–454. https://doi.org/10.3852/09-164
Vellinga, E.C., Sysouphanthong, P. & Hyde, K.D. (2011) The family Agaricaceae: phylogenies and two new white-spored genera. Mycologia, 103: 494–509. https://doi.org/10.3852/10-204
Vizzini, A., Lezzi, T., Tatti, A., Iannootti, M., Filippa, M. & Dovana, F. (2017) Molecular confirmation of Leucoagaricus idae-fragum (Agaricales, Agaricaceae), and notes on its morphological variability. Phytotaxa 332: 157–171. https://doi.org/10.11646/phytotaxa.332.2.3
White, T.J., Bruns, T., Lee, S. & Taylor, J.W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR protocols: a guide to methods and applications. Academic Press, New York, pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1