Abstract
The Krka River is located at Balkan Peninsula in the Dinaric region of Croatia and it is a typical groundwater-fed karst river characterized by an unusual phenomenon-tufa. Tufa designates porous CaCO3 deposits forming under specific physical and chemical conditions and hosting very diverse freshwater biota. Karst springs are considered one of the most diverse habitats for diatoms and are usually inhabited by specific diatom assemblages, which include the Krka spring zone. However, such habitats are particularly impacted by human activities, so previous studies have recently been conducted to raise awareness of the importance of karstic spring zones, especially because they are inhabited by specific and rare species. During the research at Krka River, samples were collected in the spring zone in May 2017, and the observations revealed the existence of a one Cymbopleura species characterized by slightly convex to almost linear valves, which are slightly protracted, broadly rounded and weakly dorsally bent at the apices. Based on detailed morphological analyses on light and scanning electron microscopy it appears that this population resembles two previously described taxa: C. rupicola var. minor Krammer and C. rupicola var. korana Krammer. Here we provide a more detailed description of the mentioned population under the new name Cymbopleura amicula stat. nov. et nom. nov. due to the significant differences from C. rupicola, together with a comparison with similar taxa in Cymbopleura.
References
Andersen, T., Baranov, V., Hagenlund, L.K., Ivkovi?, M., Kvifte, G.M. & Pavlek, M. (2016) Blind Flight? A New Troglobiotic Orthoclad (Diptera, Chironomidae) from the Lukina Jama - Trojama Cave in Croatia. PLOS ONE 11: e0152884. https://doi.org/10.1371/journal.pone.0152884
Bahls, L. (2014) New diatoms from the American West--A tribute to citizen science. Proceedings of the Academy of Natural Sciences of Philadelphia 163: 61–84. http://dx.doi.org/10.1635/053.163.0109
Bahls, L.L. (2019) Diatoms from Western North America. 2. Cymbellafalsa, Cymbopleura and Delicatophycus (Bacillariophyta)—Taxonomy, Ecology, Biogeography. The Montana Diatom Collection, Helena, Montana, 114 pp. [ISBN 978-1-7923-0988-5]
Bahls, L. & Luna, T. (2018) Cymbopleura laszlorum spec. nov. (Cymbellaceae, Bacillariophyceae), a glacial relic from a calcium-rich floodplain fen in southwestern Montana, USA. Phytotaxa 349: 47–53. https://doi.org/10.11646/phytotaxa.349.1.5
Bakalowicz, M. (2005) Karst groundwater: a challenge for new resources. Hydrogeology Journal 13: 148–160. https://doi.org/10.1007/s10040-004-0402-9
Bonacci, O. (1985) Hydrological investigations of Dinaric karst at the Krcic catchment and River Krka Springs (Yugoslavia). Journal of Hydrology 82: 317–326. https://doi.org/10.1016/0022-1694(85)90024-1
Bonacci, O. (1987) Karst hydrology with special reference to Dinaric karst. Springer, Verlag, Berlin, 184 pp.
Bonacci, O. (1995) Hidrologija malih voda Skradinskog buka na rijeci Krki. Hrvatska Vodoprivreda 4 (28): 22–26. [in Croatian]
Bonacci, O. & Jelin, J. (1988) Identification of a karst hydrological system in the Dinaric karst (Yugoslavia). Hydrological Sciences Journal 33: 483–497. https://doi.org/10.1080/02626668809491276
Bonacci, O. & Ljubenkov, I. (2005) Nove spoznaje o hidrologiji rijeke Krke. Hrvatske Vode 13 (52): 265–281. [in Croatian]
Bonacci, O., Juki?, D. & Ljubenkov, I. (2006) Definition of catchment area in karst: Case of the 2rivers Kr?i? and Krka, Croatia. Hydrological Sciences Journal 51 (4): 682–699. https://doi.org/10.1623/hysj.51.4.682
Bonacci, O., Željkovi?, I. & Gali?, A. (2012) Karst rivers’ particularity: An example from Dinaric karst (Croatia/Bosnia and Herzegovina). Environmental Earth Sciences 70: 963–974. https://doi.org/10.1007/s12665-012-2187-9
Cantonati, M., Füreder, L., Gerecke, R., Jüttner, I. & Cox, E. (2012a) Crenic habitats, hotspots for freshwater biodiversity conservation: Toward an understanding of their ecology. Journal of the North American Benthological Society 31: 463–480. https://doi.org/10.1899/11-111.1
Cantonati, M., Rott, E., Spitale, D., Angeli, N. & Komárek, J. (2012b) Are benthic algae related to spring types? Journal of the North American Benthological Society 31: 481–498. https://doi.org/10.1899/11-048.1
Cantonati, M., Segadelli, S., Ogata, K., Tran, H., Sanders, D., Gerecke, R., Rott, E., Filippini, M., Gargini, A. & Celico, F. (2016) A global review on ambient Limestone-Precipitating Springs (LPS): Hydrogeological setting, ecology, and conservation. Science of The Total Environment 568: 624–637. https://doi.org/10.1016/j.scitotenv.2016.02.105
Cantonati, M., Segadelli, S., Spitale, S., Gabrieli, J., Gerecke, G., Angeli, N., De Nardo, M.T., Ogata, K. & Wehr, J.D. (2020a) Geological and hydrochemical prerequisites of unexpectedly high biodiversity in spring ecosystems at the landscape level. Science of The Total Environment 740: 140157. https://doi.org/10.1016/j.scitotenv.2020.140157
Cantonati, M., Poikane, S., Pringle, C.M., Stevens, L.E., Turak, E., Heino, J., Richardson, J.S., Bolpagni, R., Borrini, A., Cid, N., ?tvrtlíková, M., Galassi, D.M.P., Hájek, M., Hawes, I., Levkov, Z., Naselli-Flores, L., Saber, A.A., Cicco, M. Di, Fiasca, B., Hamilton, P.B., Kube?ka, J., Segadelli, S. & Znachor, P. (2020b) Characteristics, Main Impacts, and Stewardship of Natural and Artificial Freshwater Environments: Consequences for Biodiversity Conservation. Water 12 (1): 260. http://dx.doi.org/10.3390/w12010260
Caput Mihali?, K., Gligora Udovi?, M., Galovi?, I., Stankovi?, I., Šušnjara, M., Žutini?, P., Kulaš, A., Špoljari?, I. & Levkov, Z. (2019) Tetramphora croatica sp. nov.—A new brackish-water species from Lake Vransko, Croatia. Phytotaxa 401 (4): 276–286. https://doi.org/10.11646/phytotaxa.401.4.5
Caput Mihali?, K., Galovi?, I., Wetzel, C., Ector, L., Ilijani?, N., Miko, S., Wishkerman, A., Hamilton, P. & Levkov, Z. (2020) Envekadea vranaensis sp. nov. a new diatom species (Bacillariophyta) from the lacustrine Holocene sediments of Lake Vrana, Croatia. Nova Hedwigia 110: 1–19. https://doi.org/10.1127/nova_hedwigia/2019/0561
Cukrov, N., Cmuk, P., Kwokal, Ž., Marguš, D., Mlakar, M., Omanovi?, D. (2007) Ecotoxic trace metals in waters of “Krka” National Park - Croatia. In: Naki?, Z. (Ed.) Proceedings of second international conference on waters in protected areas. Croatian Water Pollution Control Society, Dubrovnik, pp. 226–229.
Czerwik-Marcinkowska, J., Wroblewski, W., Gradzi?ski, M. & Uher, B. (2018) Diatom species diversity and their ecological patterns on different substrates in two karstic streams in the Slovak Karst. Journal of cave and karst studies the National Speleological Society bulletin 80: 1–16. https://doi.org/10.4311/2017MB0124
Darwall, W., Carrizo, S., Numa, C., Barrios, V., Freyhof, J. & Smith, K. (2014) Freshwater Key Biodiversity Areas in the Mediterranean Basin Hotspot: Informing species conservation and development planning in freshwater ecosystems. Cambridge, UK and Malaga, Spain, IUCN, 86 pp.
De Filippi, F.M., Iacurto, S., Grelle, G. & Sappa, G. (2021) Magnesium as Environmental Tracer for Karst Spring Baseflow/Overflow Assessment—A Case Study of the Pertuso Karst Spring (Latium Region, Italy). Water 13: 93. https://doi.org/10.3390/w13010093
Delgado, C., Ector, L., Novais, M.H., Blanco, S., Hoffmann, L. & Pardo, I. (2013) Epilithic diatoms of springs and spring-fed streams in Majorca Island (Spain) with the description of a new diatom species Cymbopleura margalefii sp. nov. Fottea 13: 87–104. https://doi.org/10.5507/fot.2013.009
EU-HD (European Union Habitat Directive), (1992) Council Directive 92/43/EEC of 21 May 1992 on the conservation of natural habitats and of wild fauna and flora (EC habitats directive). Official Journal of the European Communities L 206: 7–50 (22.7.1992). [Available from: https://eur-lex.europa.eu/eli/dir/1992/43/oj]
Falasco, E., Ector, L., Ciaccio, E., Hoffmann, L. & Bona, F. (2012) Alpine freshwater ecosystems in a protected area: A source of diatom diversity. Hydrobiologia 695: 233–251. https://doi.org/10.1007/s10750-012-1114-0
Gaston, K.J. & David, R. (1994) Hotspots across Europe. Biodiversity letters 2: 108–116.
Gerecke, R., Maiolini, B. & Cantonati, M. (2007) Collecting meio- and macrozoobenthos in springs. In: Cantonati, M., Bertuzzi, E. & Spitale, D. (Eds.) The spring habitat: biota and sampling methods. Monografie del Museo Tridentino di Scienze Naturali. Museo Tridentino di Scienze Naturali, Trento, Italy, pp. 265–274.
Gligora, M., Kralj, K., Plenkovi?-Moraj, A., Hinz, F., Acs, E., Grigorszky, I., Cocquyt, C. & Van de Vijver, B. (2009) Observations on the diatom Navicula hedinii Hustedt (Bacillariophyceae) and its transfer to a new genus Envekadea Van de Vijver et al. gen. nov. European Journal of Phycology 44: 123–138. https://doi.org/10.1080/09670260802389783
Gligora Udovi?, M., Žutini?, P., Kavre Piltaver, I., Kulaš, A., Ozimec, R. & Tofilovska, S. (2018) Gomphosphenia plenkoviciae sp. nov. A new species from Crveno jezero, Croatia. Phytotaxa 351: 229–238. https://doi.org/10.11646/phytotaxa.351.3.4
Griffiths, H., Krystufek, B. & Reed, J. (2004) Balkan Biodiversity: Pattern and Process in the European Hotspot. Springer Nature, 357 pp. https://doi.org/10.1007/978-1-4020-2854-0
Hofmann, G., Lange-Bertalot, H., Werum, M., Klee, R., (2018) Rote Liste und Gesamtartenliste der limnischen Kieselalgen (Bacillariophyta) Deutschlands, in: Metzing, D., Hofbauer, N., Ludwig, G., Matzke-Hajek, G. (Eds.) Rote Liste gefährdeter Tiere, Pflanzen und Pilze Deutschlands, Band 7: Pflanzen. Landwirtschaftsverlag Münster, Naturschutz und Biologische Vielfalt, Münster, pp, 601–708.
Ivkovi?, M., Plant, A. & Horvat, B. (2012) A new species of Wiedemannia (Diptera: Empididae: Clinocerinae) from Balkan Peninsula. Zootaxa 3478: 581–585. https://doi.org/10.11646/zootaxa.3478.1.46
Krammer, K. (1997) Die cymbelloiden Diatomeen. Eine Monographie der weltweit bekannten Taxa. Teil 2. Encyonema Part., Encyonopsis und Cymbellopsis. Bibliotheca Diatomologica 37: 1–469.
Krammer, K. (1999) Validierung von Cymbopleura nov. gen. In: Lange-Bertalot, H. (Ed.) Iconographia Diatomologica 6. Koeltz Botanical Books, Königstein, Germany, pp. 292.
Krammer, K. (2002) Cymbella. In: Lange-Bertalot, H. (Ed.) Diatoms of Europe: Diatoms of the European Inland Waters and Comparable Habitats 3. A.R.G. Gantner Verlag K.G., Ruggell. pp. 1–584.
Krammer, K. (2003) Cymbopleura, Delicata, Navicymbula, Gomphocymbellopsis, Afrocymbella. In: Lange-Bertalot, H. (Ed.) Diatoms of Europe: Diatoms of the European Inland Waters and Comparable Habitats 4. A.R.G. Gantner Verlag K.G., Ruggell. pp. 1–530.
Krammer, K. & Lange-Bertalot, H. (1985) Naviculaceae Neue und wenig bekannte Taxa, neue Kombinationen und Synonyme sowie Bemerkungen zu einigen Gattungen. Bibliotheca Diatomologica 9: 5–230.
Kobayasi, H. (1997) Comparative studies among four linear-lanceolate Achnanthidium species (Bacillariophyceae) with curved terminal raphe endings. Nova Hedwigia 65: 147–163. https://dx.doi.org/10.1127/0029-5035/2006/0082-0399
Kulaš, A., Gligora Udovi?, M., Ector, L. & Van de Vijver, B. (2020) Analysis of the type material of Achnanthes hauckiana Grunow (Achnanthaceae, Bacillariophyceae). Botany Letters 167 (4): 439–452. https://doi.org/10.1080/23818107.2020.1808527
Kützing, F.T. (1833) Synopsis Diatomacearum oder Versuch einer systematischen Zusammenstellung der Diatomeen. Vol. 8. Linnaea, 529–620. https://doi.org/10.5962/bhl.title.65634
Kvifte, G. & Ivkovi?, M. (2018) New species and records of the Pericoma trifasciata group from Croatia (Diptera: Psychodidae). Zootaxa 4486: 76–82. https://doi.org/10.11646/zootaxa.4486.1.5
Kvifte, G., Ivkovi?, M. & Klari?, A. (2013) New records of moth flies (Diptera: Psychodidae) from Croatia, with the description of Berdeniella keroveci sp.nov. Zootaxa 3737: 57–67. https://doi.org/10.11646/zootaxa.3737.1.4
Lai, G.G., Burato, S., Padedda, B.M., Zorza, R., Pizzul, E., Delgado, C., Lugliè, A. & Cantonati, M. (2019) Diatom Biodiversity in Karst Springs of Mediterranean Geographic Areas with Contrasting Characteristics: Islands vs Mainland. Water 11 (12): 2602. https://doi.org/10.3390/w11122602
Le Cohu, R., Lange-Bertalot, H. & Azémar, F. (2011) Cymbopleura pyrenaica sp. nov. (Bacillariophyceae) et d’autres Espèces du Même Genre Rarement Recensées Dans Quelques Lacs des Pyrénées Françaises. Cryptogamie Algologie 32: 337–349. https://doi.org/10.7872/crya.v32.iss4.2011.337
Levkov, Z., Krstic, S., Nakov, T. & Melovski, L. (2005) Diatom assemblages on Shara and Nidze Mountains, Macedonia. Nova Hedwigia 81: 501–538. https://doi.org/10.1127/0029-5035/2005/0081-0501
Lowe, R., Kociolek, P., Qingmin, Y., Wang, Q. & Stepanek, J. (2017) Diversity of the diatom genus Humidophila in karst areas of Guizhou, China. Phytotaxa 305: 269–284. https://doi.org/10.11646/phytotaxa.305.4.3
Mar?i?, Z., Buj, I., Dupli?, A., ?aleta, M., Mustafi?, P., Zanella, D., Zupan?i?, P. & Mrakov?i?, M. (2011) A new endemic cyprinid species from the Danube drainage. Journal of Fish Biology 79: 418–430. https://doi.org/https://doi.org/10.1111/j.1095-8649.2011.03038.x
Mogna, M., Cantonati, M., Andreucci, F., Angeli, N., Berta, G. & Miserere, L. (2015) Diatom communities and vegetation of springs in the south-western Alps. Acta Botanica Croatica 74 (2). https://doi.org/10.1515/botcro-2015-0024
Mooney, H.A. (1988) Lessons from Mediterranean-climate regions. In: Wilson, E.O. & Peter, F.M. (Eds.) Biodiversity. National Academies Press, Washington, USA, pp. 157–165.
Mustafi?, P., Mar?i?, Z., Dupli?, A., Mrakov?i?, M., ?aleta, M., Zanella, D., Buj, I., Podnar, M. & Dolenec, Z. (2008) A new loach species of the genus Cobitis in Croatia. Folia Zoologica -Praha 57 (1–2): 4–9.
Myers, N., Mittermeier, R.A., Mittermeier, C.G., da Fonseca, G.A.B. & Kent, J. (2000) Biodiversity hotspots for conservation priorities. Nature 403: 853–858. https://doi.org/10.1038/35002501
Oosterbroek, P. (1994) Biodiversity of the Mediterranean Region. In: Forey, P.L.; Humphries, C.J.; Vane-Wright, R.I. (Eds.) Systematics and Conservation Evaluation. Systematic Association Special Volume 50: 289–307.
Plenkovi?-Moraj, A. (1995) Diatoms (Bacillariophyceae) of the Croatian Freshwater. Acta Botanica Croatica 54: 23–33.
Pont, A.C. & Ivkovi?, M. (2013) The hunter-flies of Croatia (Diptera: Muscidae: genus Limnophora Robineau-Desvoidy). Journal of Natural History 47: 1069–1082. https://doi.org/10.1080/00222933.2012.750775
Previši?, A., Schnitzler, J., Ku?ini?, M., Wolfram, G., Ibrahimi, H., Kerovec, M. & Pauls, S. (2014a) Microscale Vicariance and Diversification of Western Balkan Caddisflies Linked to Karstification. Freshwater science 33: 250–262. https://doi.org/10.1086/674430
Previši?, A., Wolfram, G., Vitecek, S., Ku?ini?, M., Bálint, M., Keresztes, L., Pauls, S. & Waringer, J. (2014b) Cryptic diversity of caddisflies in the Balkans: The curious case of Ecclisopteryx species (Trichoptera: Limnephilidae). Arthropod Systematics and Phylogeny 72: 309–329.
Primc-Habdija, B. & Matoni?kin, R. (2005) A new freshwater folliculinid (Ciliophora) from the karstic region of Croatia. European Journal of Protistology 41: 37–43. https://doi.org/10.1016/j.ejop.2004.09.004
Rubini?, J., Goreta, G., Giljuši?, M., Marguš, D., Radiši?, M., Zwicker Kompar, G. & Kompar, D. (2017) Hidrologija Krke -utjecaji i promjene. In: Marguš, D. (Ed.) Zbornik Radova: Vizija i Izazovi Upravljanja Zašti?enim Podru?jima Prirode u Republici Hrvatskoj. Javna ustanova “Nacionalni park Krka”, Šibenik, Croatia, pp. 705–720.
Šiljeg, A., Mari?, I., Cukrov, N., Domazetovi?, F. & Roland, V. (2020) A Multiscale Framework for Sustainable Management of Tufa-Forming Watercourses: A Case Study of National Park “Krka”, Croatia. Water 12 (11): 3096. https://doi.org/10.3390/w12113096
Tamburini, A. & Menichetti, M. (2020) Groundwater Circulation in Fractured and Karstic Aquifers of the Umbria-Marche Apennine. Water 12 (4): 1039. https://doi.org/10.3390/w12041039
Taxböck, L., Linder, H. & Cantonati, M. (2017) To What Extent Are Swiss Springs Refugial Habitats for Sensitive and Endangered Diatom Taxa? Water 9: 967. https://doi.org/10.3390/w9120967
Tierno de Figueroa, J.M., López-Rodríguez, M.J., Fenoglio, S., Sánchez-Castillo, P. & Fochetti, R. (2013) Freshwater biodiversity in the rivers of the Mediterranean Basin. Hydrobiologia 719: 137–186. https://doi.org/10.1007/s10750-012-1281-z
Van de Vijver, B., Jüttner, I., Gurung, S., Sharma, C., Sharma, S., de Haan, M. & Cox, E. (2011) The genus Cymbopleura (Cymbellales, Bacillariophyta) from high altitude freshwater habitats, Everest National Park, Nepal, with the description of two new species. Fottea 11: 245–269. https://doi.org/10.5507/fot.2011.025
Vilenica, M., Gattolliat, J.-L., Ivkovi?, M., Ku?ini?, M., Mi?eti? Stankovi?, V., Mihaljevi?, Z. & Sartori, M. (2014) The Mayfly fauna (Insecta, Ephemeroptera) of the Plitvice Lakes National Park, Croatia. Natura Croatica 23: 349–363.
Vilenica, M., Mi?eti? Stankovi?, V., Sartori, M., Ku?ini?, M. & Mihaljevi?, Z. (2017) Environmental factors affecting mayfly assemblages in tufa-depositing habitats of the Dinaric Karst. Knowledge and Management of Aquatic Ecosystems 418 (14): 1–12. https://doi.org/10.1051/kmae/2017005
Vilenica, M., Bili?, M., Mi?eti? Stankovi?, V. & Ku?ini?, M. (2018a) Mayfly ecological traits in a European karst spring: Species, microhabitats and life histories. Community Ecology 19: 248–258. https://doi.org/10.1556/168.2018.19.3.6
Vilenica, M., Mi?eti? Stankovi?, V., Mihaljevi?, Z. & Ku?ini?, M. (2018b) Alderfly assemblages (Megaloptera, Sialidae) along an oligotrophic karst hydrosystem: spatial patterns and species-environment relationship. Biologia 73: 493–503. https://doi.org/10.2478/s11756-018-0056-z
Werum, M. & Lange-Bertalot, H. (2004) Diatoms in springs from Central Europe and elsewhere under the influence of hydrologeology and anthropogenic impacts. Iconographia Diatomologica 13: 3–417.
Žuljevi?, A., Kaleb, S., Peña, V., Despalatovi?, M., Cvitkovi?, I., De Clerck, O., Le Gall, L., Falace, A., Vita, F., Braga, J.C. & Antoli?, B. (2016) First freshwater coralline alga and the role of local features in a major biome transition. Scientific Reports 6: 19642. https://doi.org/10.1038/srep19642
Žutini?, P., Kulaš, A., Levkov, Z., Šušnjara, M., Orli?, S., Kuki?, S., Goreta, G., Vali?, D. & Gligora Udovi?, M. (2020) Ecological status assessment using periphytic diatom communities – case study Krka River. Macedonian Journal of Ecology and Environment 22 (1): 29–44.
