Abstract
During an investigation of freshwater fungi from the Greater Mekong Subregion, a Bactrodesmium-like taxon was collected from a freshwater habitat in Yunnan Province, China. Phylogenetic analysis of combined LSU, SSU, ITS, TEF1-α and RPB2 sequence data placed the new isolate, Neoascotaiwania aquatica, in the genus Neoascotaiwania within Savoryellaceae. The new taxon is distinguished from other Neoascotaiwania species by its semi-macronematous, uniseptate, subhyaline to pale brown conidiophores and fusiform, 2-septate conidia.
References
Boonyuen, N., Chuaseeharonnachai, C., Suetrong, S., Sri-Indrasutdhi, V., Sivichai, S., Jones, E.B.G. & Pang, K.L. (2011) Savoryellales (Hypocreomycetidae, Sordariomycetes): a novel lineage of aquatic ascomycetes inferred from multiple-gene phylogenies of the genera Ascotaiwania, Ascothailandia and Savoryella. Mycologia 103 (6): 1351–1350. https://doi.org/10.3852/11-102
Cai, L., Jeewon, R. & Hyde, K.D. (2006) Phylogenetic investigations of Sordariaceae based on multiple gene sequences and morphology. Mycological Research 110: 137–150. https://doi.org/10.1016/j.mycres.2005.09.014
Chang, H.S., Hsieh, S.Y., Jones, E.B.G., Read, S.J. & Moss, S.T. (1998) New freshwater species of Ascotaiwania and Savoryella from Taiwan. Mycological Research 102 (6): 709?718. https://doi.org/10.1017/S0953756297005637
Chomnunti, P., Hongsanan, S., Aguirre-Hudson, B., Tian, Q., Peršoh, D., Dhami, M.K., Alias, A.S., Xu, J., Liu, X. & Stadler, M. (2014) The sooty moulds. Fungal Diversity 66: 1–36. https://doi.org/10.1007/s13225-014-0278-5
Dayarathne, M.C., Maharachchikumbura, S.S.N., Jones, E.B.G., Dong, W., Devadatha, B., Yang, J., Ekanayaka, A.H., Silva, W.D., Sarma, V.V., Al-Sadi, A.M., Khongphinitbunjong, K., Kitiphong, K., Hyde, K.D. & Zhao, R.L. (2019) Phylogenetic revision of Savoryellaceae and evidence for its ranking as a subclass. Frontiers in Microbiology 10: 1–26. https://doi.org/10.3389/fmicb.2019.00840
Dong, W., Wang, B., Hyde, K.D., McKenzie, E.H.C., Bhat, D.J., Raja, H.A., Tanaka, K., Abdel-Wahab, M.A., Abdel-Aziz, F.A., Doilom, M., Phookamsak, R., Hongsanan, S., Wanasinghe, D.N., Yu, X.D., Wang, G.N., Yang, H., Yang, J., Thambugala, A.N., Tian, Q., Luo, Z.L., Yang, J.B., Miller, A.N., Fournier, J., Boonmee, S., Hu, D.M., Nalumpang, S. & Zhang, H. (2020) Freshwater Dothideomycetes. Fungal Diversity 105: 319–575. https://doi.org/10.1007/s13225-020-00463-5
Eriksson, O.E. & Hawksworth, D.L. (1986) An alphabetical list of the generic names of ascomycetes. Systema Ascomycetum 5: 3–111.
Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.
Hernández-Restrepo, M., Gené, J., Castañeda-Ruiz, R.F., Mena-Portales, J., Crous, P.W. & Guarro, J. (2017) Phylogeny of saprobic microfungi from Southern Europe. Studies in Mycology 86: 53?97. https://doi.org/10.1016/j.simyco.2017.05.002
Index Fungorum (2021) Available online: http://www.indexfungorum.org/Names/Names.asp (accessed 30 August 2021)
Jaklitsch, W.M. & Réblová, M. (2015) Savoryellaceae Jaklitsch & Réblová. Index Fungorum 209: 1.
Jayasiri, S.C., Hyde, K.D., Ariyawansa, H.A., Bhat, D.J., Buyck, B., Cai, L., Dai, Y.C., Abd-Elsalam, K.A., Ertz, D., Hidayat, I., Jeewon, R., Jones, E.B.G., Bahkali, A.H., Karunarathna, S.C., Liu, J.K., Luangsa-ard, J.J., Lumbsch, H.T., Maharachchikumbura, S.S.N., McKenzie, E.H.C., Moncalvo, J.M., Ghobad-Nejhad, M., Nilsson, H., Pang, K.A., Pereira, O.L., Phillips, A.J.L., Raspé, O., Rollins, A.W., Romero, A.I., Etayo, J., Selçuk, F., Stephenson, S.L., Suetrong, S., Taylor, J.E., Tsui, C.K.M., Vizzini, A., Abdel-Wahab, M.A., Wen, T.C., Boonmee, S., Dai, D.Q., Daranagama, D.A., Dissanayake, A.J., Ekanayaka, A.H., Fryar, S.C., Hongsanan, S., Jayawardena, R.S., Li, W.J., Perera, R.H., Phookamsak, R., de Silva, N.I., Thambugala, K.M., Tian, Q., Wijayawardene, N.N., Zhao, R.L., Zhao, Q., Kang, J.C. & Promputtha, I. (2015) The faces of fungi database: fungal names linked with morphology, phylogeny and human impacts. Fungal Diversity 74: 3–18. https://doi.org/10.1007/s13225-015-0351-8
Jeewon, R. & Hyde, K.D. (2016) Establishing species boundaries and new taxa among fungi: recommendations to resolve taxonomic ambiguities. Mycosphere 7: 1669–1677. https://doi.org/10.5943/mycosphere/7/11/4
Jones, E.B.G. & Hyde, K.D. (1992) Taxonomic studies on Savoryella Jones et Eaton (Ascomycotina). Botanica Marina 35: 83–91. https://doi.org/10.1515/botm.1992.35.2.83
Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30: 772–780. https://doi.org/10.1093/molbev/mst010
Kohlmeyer, J. & Kohlmeyer, E. (1979) Marine Mycology: the higher fungi. Academic Press, New York, 690 pp.
Liu, J.K., Phookamsak, R., Doilom, M., Wikee, S., Li, Y.M., Ariyawansha, H., Boonmee, S., Chomnunti, P., Dai, D.Q., Bhat, D.J., Romero, A.I., Zhuang, W.Y., Monkai, J., Jones, E.B.G., Chukeatirote, E., Ko Ko, T.W., Zhao, Y.C., Wang, Y. & Hyde, K.D. (2012) Towards a natural classification of Botryosphaeriales. Fungal Diversity 57: 149–210. https://doi.org/10.1007/s13225-012-0207-4
Liu, Y.J., Whelen, S. & Hall, B.D. (1999) Phylogenetic relationships among ascomycetes: evidence from an RNA polymerase II subunit. Molecular Biology and Evolution 16: 1799–1808. https://doi.org/10.1093/oxfordjournals.molbev.a026092
Luo, Z.L., Hyde, K.D., Liu, J.K., Bhat, D.J., Bao, D.F., Li, W.L. & Su, H.Y. (2018) Lignicolous freshwater fungi from China II: Novel Distoseptispora (Distoseptisporaceae) species from northwestern Yunnan Province and a suggested unified method for studying lignicolous freshwater fungi. Mycosphere 9: 444–461. https://doi.org/10.5943/mycosphere/9/3/2
Luo, Z.L., Hyde, K.D., Liu, J.K., Maharachchikumbura, S.S.N., Jeewon, R., Bao, D.F., Bhat, D.J., Lin, C.G., Li, W.L., Yang, J., Liu, N.G., Lu, Y.Z., Jayawardena, R.S., Li, J.F. & Su, H.Y. (2019) Freshwater Sordariomycetes. Fungal Diversity 99: 451–660. https://doi.org/10.1007/s13225-019-00438-1
Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: Proceedings of the 2010 gateway Computing Environments Workshop (gCE), November 2010. New Orleans, Louisiana, pp. 1–8. https://doi.org/10.1109/GCE.2010.5676129
Nylander, J.A.A. (2004) MrModeltest 2.0. Program distributed by the author. Evolutionary Biology Centre, Uppsala University.
Rannala, B. & Yang, Z. (1996) Probability distribution of molecular evolutionary trees: a new method of phylogenetic inference. Journal of Molecular Evolution 43: 304–311. https://doi.org/10.1007/BF02338839
Read, S.J., Jones, E.B.G. & Moss, S.T. (1993) Taxonomic studies of marine Ascomycotina: ultrastructure of the asci, ascospores and appendages of Savoryella species. Canadian Journal of Botany 71: 273–283. https://doi.org/10.1139/b93-028
Réblová, M., Seifert, K.A., Fournier, J. & Št?pánek, V. (2016) Newly recognised lineages of perithecial ascomycetes: the new orders Conioscyphales and Pleurotheciales. Persoonia 37: 57–81. http://dx.doi.org/10.3767/003158516X689819
Réblová, M., Hernández-Restrepo, M. & Fournier, J. (2020) New insights into the systematics of Bactrodesmium and its allies and introducing new genera, species and morphological patterns in the Pleurotheciales and Savoryellales (Sordariomycetes). Studies in Mycology 95: 415–466. https://doi.org/10.1016/j.simyco.2020.02.002
Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572. https://doi.org/10.1093/bioinformatics/btg180
Stamatakis, A. (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22: 2688–2690. https://doi.org/10.1093/bioinformatics/btl446
Stamatakis, A., Hoover, P. & Rougemont, J. (2008) A rapid bootstrap algorithm for the RAxML web-servers. Systematic Biology 75: 758–771. https://doi.org/10.1080/10635150802429642
Vijaykrishna, D., Jeewon, R. & Hyde, K.D. (2006) Molecular taxonomy, origins and evolution of freshwater ascomycetes. Fungal Diversity 23: 351–390.
Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
Wei, M.J., Zhang, H., Dong, W., Boonmee, S. & Zhang, D. (2018) Introducing Dictyochaeta aquatica sp. nov. and two new species of Chloridium (Chaetosphaeriaceae, Sordariomycetes) from aquatic habitats. Phytotaxa 362: 187–199. https://doi.org/10.11646/phytotaxa.362.2.5
White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, G.M., Shinsky, D. & White, T. (Eds.) PCR protocols: a guide to methods and applications 18: 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
Yang, J., Maharachchikumbura, S.S.N., Bhat, D.J., Hyde, K.D., Mckenzie, E.H.C., Jones, E.B.G, Al-Sadi, A.M. & Lumyong, M. (2016) Fuscosporellales, a new order of aquatic and terrestrial Hypocreomycetidae (Sordariomycetes). Cryptogamie Mycologie 37: 449–475. https://doi.org/10.7872/crym/v37.iss4.2016.449
Zhang, H., Dong, W., Hyde, K.D., Maharachchikumbura, S.S.N., Hongsanan, S., Jayarama Bhat, D., Al-Sadi, A.M. & Zhang, D. (2017) Towards a natural classification of Annulatascaceae-like taxa: introducing Atractosporales ord. nov. and six new families. Fungal Diversity 85: 75–110. https://doi.org/10.1007/s13225-017-0387-z
Zhang, S.N., Abdel-Wahab, M.A., Jones, E.B.G., Hyde, K.D. & Liu, J.K. (2019) Additions to the genus Savoryella (Savoryellaceae), with the asexual morphs Savoryella nypae comb. nov. and S. sarushimana sp. nov. Phytotaxa 408: 195–207 https://doi.org/10.11646/phytotaxa.408.3.4
Cai, L., Jeewon, R. & Hyde, K.D. (2006) Phylogenetic investigations of Sordariaceae based on multiple gene sequences and morphology. Mycological Research 110: 137–150. https://doi.org/10.1016/j.mycres.2005.09.014
Chang, H.S., Hsieh, S.Y., Jones, E.B.G., Read, S.J. & Moss, S.T. (1998) New freshwater species of Ascotaiwania and Savoryella from Taiwan. Mycological Research 102 (6): 709?718. https://doi.org/10.1017/S0953756297005637
Chomnunti, P., Hongsanan, S., Aguirre-Hudson, B., Tian, Q., Peršoh, D., Dhami, M.K., Alias, A.S., Xu, J., Liu, X. & Stadler, M. (2014) The sooty moulds. Fungal Diversity 66: 1–36. https://doi.org/10.1007/s13225-014-0278-5
Dayarathne, M.C., Maharachchikumbura, S.S.N., Jones, E.B.G., Dong, W., Devadatha, B., Yang, J., Ekanayaka, A.H., Silva, W.D., Sarma, V.V., Al-Sadi, A.M., Khongphinitbunjong, K., Kitiphong, K., Hyde, K.D. & Zhao, R.L. (2019) Phylogenetic revision of Savoryellaceae and evidence for its ranking as a subclass. Frontiers in Microbiology 10: 1–26. https://doi.org/10.3389/fmicb.2019.00840
Dong, W., Wang, B., Hyde, K.D., McKenzie, E.H.C., Bhat, D.J., Raja, H.A., Tanaka, K., Abdel-Wahab, M.A., Abdel-Aziz, F.A., Doilom, M., Phookamsak, R., Hongsanan, S., Wanasinghe, D.N., Yu, X.D., Wang, G.N., Yang, H., Yang, J., Thambugala, A.N., Tian, Q., Luo, Z.L., Yang, J.B., Miller, A.N., Fournier, J., Boonmee, S., Hu, D.M., Nalumpang, S. & Zhang, H. (2020) Freshwater Dothideomycetes. Fungal Diversity 105: 319–575. https://doi.org/10.1007/s13225-020-00463-5
Eriksson, O.E. & Hawksworth, D.L. (1986) An alphabetical list of the generic names of ascomycetes. Systema Ascomycetum 5: 3–111.
Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.
Hernández-Restrepo, M., Gené, J., Castañeda-Ruiz, R.F., Mena-Portales, J., Crous, P.W. & Guarro, J. (2017) Phylogeny of saprobic microfungi from Southern Europe. Studies in Mycology 86: 53?97. https://doi.org/10.1016/j.simyco.2017.05.002
Index Fungorum (2021) Available online: http://www.indexfungorum.org/Names/Names.asp (accessed 30 August 2021)
Jaklitsch, W.M. & Réblová, M. (2015) Savoryellaceae Jaklitsch & Réblová. Index Fungorum 209: 1.
Jayasiri, S.C., Hyde, K.D., Ariyawansa, H.A., Bhat, D.J., Buyck, B., Cai, L., Dai, Y.C., Abd-Elsalam, K.A., Ertz, D., Hidayat, I., Jeewon, R., Jones, E.B.G., Bahkali, A.H., Karunarathna, S.C., Liu, J.K., Luangsa-ard, J.J., Lumbsch, H.T., Maharachchikumbura, S.S.N., McKenzie, E.H.C., Moncalvo, J.M., Ghobad-Nejhad, M., Nilsson, H., Pang, K.A., Pereira, O.L., Phillips, A.J.L., Raspé, O., Rollins, A.W., Romero, A.I., Etayo, J., Selçuk, F., Stephenson, S.L., Suetrong, S., Taylor, J.E., Tsui, C.K.M., Vizzini, A., Abdel-Wahab, M.A., Wen, T.C., Boonmee, S., Dai, D.Q., Daranagama, D.A., Dissanayake, A.J., Ekanayaka, A.H., Fryar, S.C., Hongsanan, S., Jayawardena, R.S., Li, W.J., Perera, R.H., Phookamsak, R., de Silva, N.I., Thambugala, K.M., Tian, Q., Wijayawardene, N.N., Zhao, R.L., Zhao, Q., Kang, J.C. & Promputtha, I. (2015) The faces of fungi database: fungal names linked with morphology, phylogeny and human impacts. Fungal Diversity 74: 3–18. https://doi.org/10.1007/s13225-015-0351-8
Jeewon, R. & Hyde, K.D. (2016) Establishing species boundaries and new taxa among fungi: recommendations to resolve taxonomic ambiguities. Mycosphere 7: 1669–1677. https://doi.org/10.5943/mycosphere/7/11/4
Jones, E.B.G. & Hyde, K.D. (1992) Taxonomic studies on Savoryella Jones et Eaton (Ascomycotina). Botanica Marina 35: 83–91. https://doi.org/10.1515/botm.1992.35.2.83
Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30: 772–780. https://doi.org/10.1093/molbev/mst010
Kohlmeyer, J. & Kohlmeyer, E. (1979) Marine Mycology: the higher fungi. Academic Press, New York, 690 pp.
Liu, J.K., Phookamsak, R., Doilom, M., Wikee, S., Li, Y.M., Ariyawansha, H., Boonmee, S., Chomnunti, P., Dai, D.Q., Bhat, D.J., Romero, A.I., Zhuang, W.Y., Monkai, J., Jones, E.B.G., Chukeatirote, E., Ko Ko, T.W., Zhao, Y.C., Wang, Y. & Hyde, K.D. (2012) Towards a natural classification of Botryosphaeriales. Fungal Diversity 57: 149–210. https://doi.org/10.1007/s13225-012-0207-4
Liu, Y.J., Whelen, S. & Hall, B.D. (1999) Phylogenetic relationships among ascomycetes: evidence from an RNA polymerase II subunit. Molecular Biology and Evolution 16: 1799–1808. https://doi.org/10.1093/oxfordjournals.molbev.a026092
Luo, Z.L., Hyde, K.D., Liu, J.K., Bhat, D.J., Bao, D.F., Li, W.L. & Su, H.Y. (2018) Lignicolous freshwater fungi from China II: Novel Distoseptispora (Distoseptisporaceae) species from northwestern Yunnan Province and a suggested unified method for studying lignicolous freshwater fungi. Mycosphere 9: 444–461. https://doi.org/10.5943/mycosphere/9/3/2
Luo, Z.L., Hyde, K.D., Liu, J.K., Maharachchikumbura, S.S.N., Jeewon, R., Bao, D.F., Bhat, D.J., Lin, C.G., Li, W.L., Yang, J., Liu, N.G., Lu, Y.Z., Jayawardena, R.S., Li, J.F. & Su, H.Y. (2019) Freshwater Sordariomycetes. Fungal Diversity 99: 451–660. https://doi.org/10.1007/s13225-019-00438-1
Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: Proceedings of the 2010 gateway Computing Environments Workshop (gCE), November 2010. New Orleans, Louisiana, pp. 1–8. https://doi.org/10.1109/GCE.2010.5676129
Nylander, J.A.A. (2004) MrModeltest 2.0. Program distributed by the author. Evolutionary Biology Centre, Uppsala University.
Rannala, B. & Yang, Z. (1996) Probability distribution of molecular evolutionary trees: a new method of phylogenetic inference. Journal of Molecular Evolution 43: 304–311. https://doi.org/10.1007/BF02338839
Read, S.J., Jones, E.B.G. & Moss, S.T. (1993) Taxonomic studies of marine Ascomycotina: ultrastructure of the asci, ascospores and appendages of Savoryella species. Canadian Journal of Botany 71: 273–283. https://doi.org/10.1139/b93-028
Réblová, M., Seifert, K.A., Fournier, J. & Št?pánek, V. (2016) Newly recognised lineages of perithecial ascomycetes: the new orders Conioscyphales and Pleurotheciales. Persoonia 37: 57–81. http://dx.doi.org/10.3767/003158516X689819
Réblová, M., Hernández-Restrepo, M. & Fournier, J. (2020) New insights into the systematics of Bactrodesmium and its allies and introducing new genera, species and morphological patterns in the Pleurotheciales and Savoryellales (Sordariomycetes). Studies in Mycology 95: 415–466. https://doi.org/10.1016/j.simyco.2020.02.002
Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572. https://doi.org/10.1093/bioinformatics/btg180
Stamatakis, A. (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22: 2688–2690. https://doi.org/10.1093/bioinformatics/btl446
Stamatakis, A., Hoover, P. & Rougemont, J. (2008) A rapid bootstrap algorithm for the RAxML web-servers. Systematic Biology 75: 758–771. https://doi.org/10.1080/10635150802429642
Vijaykrishna, D., Jeewon, R. & Hyde, K.D. (2006) Molecular taxonomy, origins and evolution of freshwater ascomycetes. Fungal Diversity 23: 351–390.
Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
Wei, M.J., Zhang, H., Dong, W., Boonmee, S. & Zhang, D. (2018) Introducing Dictyochaeta aquatica sp. nov. and two new species of Chloridium (Chaetosphaeriaceae, Sordariomycetes) from aquatic habitats. Phytotaxa 362: 187–199. https://doi.org/10.11646/phytotaxa.362.2.5
White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, G.M., Shinsky, D. & White, T. (Eds.) PCR protocols: a guide to methods and applications 18: 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
Yang, J., Maharachchikumbura, S.S.N., Bhat, D.J., Hyde, K.D., Mckenzie, E.H.C., Jones, E.B.G, Al-Sadi, A.M. & Lumyong, M. (2016) Fuscosporellales, a new order of aquatic and terrestrial Hypocreomycetidae (Sordariomycetes). Cryptogamie Mycologie 37: 449–475. https://doi.org/10.7872/crym/v37.iss4.2016.449
Zhang, H., Dong, W., Hyde, K.D., Maharachchikumbura, S.S.N., Hongsanan, S., Jayarama Bhat, D., Al-Sadi, A.M. & Zhang, D. (2017) Towards a natural classification of Annulatascaceae-like taxa: introducing Atractosporales ord. nov. and six new families. Fungal Diversity 85: 75–110. https://doi.org/10.1007/s13225-017-0387-z
Zhang, S.N., Abdel-Wahab, M.A., Jones, E.B.G., Hyde, K.D. & Liu, J.K. (2019) Additions to the genus Savoryella (Savoryellaceae), with the asexual morphs Savoryella nypae comb. nov. and S. sarushimana sp. nov. Phytotaxa 408: 195–207 https://doi.org/10.11646/phytotaxa.408.3.4