Abstract
A new endemic slipper orchid in the family Orchidaceae from Northern Thailand was described according to morphological and molecular analyses. Morphological information specifies the new slipper orchid Paphiopedilum charlesworthii var. lannaense to be similar to Paphiopedilum charlesworthii (Rolfe) Pfitzer 1895, with the exception of its staminode being obovate-obcordate with yellow color, glittery and rough surface. Molecular analysis by AFLP technique indicates that Paphiopedilum charlesworthii var. lannaense can be included as a member of section Paphiopedilum and is closely related to Paphiopedilum coccineum Perner, H. & Herrmann, R. (2000) (syn. Paphiopedilum barbigerum var. coccineum), from which it differs morphologically by dorsal sepal and petal characters. The morphological and molecular evidence supported that Paphiopedilum charlesworthii var. lannaense is a new slipper orchid in the genus Paphiopedilum (Cypripedioideae, Orchidaceae).
References
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<p>Wang, M., Lan, S.R. & Liu, Z.J. (2017) <em>Paphiopedilum notatisepalum</em>, a new species of slipper orchid (Cypripedioideae, Orchidaceae) from China based on morphological and DNA evidence. <em>Phytotaxa</em> 302 (2): 156–164. https://doi.org/10.11646/phytotaxa.302.2.5</p>
<p>Braem, G.J. (2016) <em>The Genus Paphiopedilum. </em>2<sup>nd</sup>. M/S Bishen Singh Mahendra Pal Singh.Dehradun, Uttarakhand, 651 pp.</p>
<p>Chen, L.J., Liu, Z.J., Li, Y.Y. & Li, L.Q. (2010) A new orchid <em>Paphiopedilum</em> <em>guangdongense</em> and its molecular evidence. <em>Systematic and Evolution</em> 48 (5): 350–355. https://doi.org/10.1111/j.1759-6831.2010.00092.x</p>
<p>Chochai, A., Leith, I.J., Ingrouille, M.J. & Fay, M.F. (2012) Molecular phylogenetics of <em>Paphiopedilum</em> (Cypripedioideae; Orchidaceae) based on nuclear ribosomal ITS and plastid sequences. <em>Botanical journal of the Linnean</em> 170: 176–196. https://doi.org/10.1111/j.1095-8339.2012.01293.x</p>
<p>Cox, A.V., Pridgeon, A.M., Albert, V.A. & Chase, M.W. (1997) Phylogenetics of the slipper orchids (Cypripedioideae, Orchidaceae): nuclear rDNA ITS sequences. <em>Plant Systematics and Evolution</em> 208: 197–223. https://doi.org/10.1007/BF00985442</p>
<p>Cribb, P.J. (1998) <em>The Genus Paphiopedilum. </em>2<sup>nd</sup>. Natural History Publication. Kota Kinabalu, Borneo, 427 pp.</p>
<p>Doyle, J.J. & Doyle, J.L. (1990) Isolation of plant DNA from fresh tissue. <em>Focus</em> 12 (1): 13–15.</p>
<p>Gruss, O. & Roellke, L (2003) <em>Paphiopedilum vejvarutianum</em>. <em>Die</em> <em>Orchidee (Hamburg)</em> 54 (1): 59.</p>
<p>Gruss, O. & Rohal, H. (2010) Breeding with recently described Vietnamense <em>Paphiopediums</em> Part III- <em>Paphiopedilum barbigerum</em> var. <em>coccineum</em>, <em>P. helenae</em>, <em>P. herrmannii</em>, <em>P. tranlienianum</em>, and <em>P. trantuanhii</em>. <em>Orchid Digest</em> 74 (3): 156–159.</p>
<p>Lee, Y.I., Chung, M.C., Sydara, K., Souliya, O. & Luang Aphay, S. (2017) Taxonomic placement of <em>Paphiopedilum rungsuriyanum</em> (Cypripedioideae; Orchidaceae) based on morphological, cytological and molecular analyses. <em>Botanical Student</em> 58: 16. https://doi.org/10.1186/s40529-017-0170-1</p>
<p>Liu, Z.J., Chen, S.C., Chen, L.J. & Lei, S.P. (2009) <em>The Genus Paphiopedilum in China</em>. Science Press. Beijing, 10 pp.</p>
<p>Min, A.A. & Tan, S.T. (1996) RAPD analysis of DNA polymorphisms among three slipper orchid species, <em>Paphiopedilum barbatum</em>, <em>Paphiopedilum callosum</em>, and <em>Paphiopedilum niveum</em>. <em>Asia-Pacific Journal of Molecular Biology and Biotechnology</em> 4 (3): 196–202.</p>
<p>Oakeley, H. & Braem, G.J. (2011) <em>Paphiopedilum</em> species the Essential Guide. Henry Oakeley Publishing. London, 86 pp.</p>
<p>Perner, H. & Herrmann, R. (2000) <em>Paphiopedilum coccineum</em> Perner et Herrmann, ein enuer Frauenschuh aus Vietnam. <em>Die Orchidee</em>. 51 (5): 622–624.</p>
<p>Salas, M.R., Ranilla, J.B. & Espinoza, J.R. (2007) Genetic relationships of <em>Phragmipedium </em>species (Orchidaceae) using amplified fragment lenght polymorphism (AFLP) analysis. <em>Lankesteriana</em> 7 (3): 493–496. [https://www.redalyc.org/articulo.oa?id=44339814004]</p>
<p>Schuiteman, A., Luang Aphay, S. & Iio, S. (2018) Paphiopedilum papilio-laoticus (Orchidaceae), a new species from Laos. <em>Orchideen Journal</em> (internet) 6 (4): 3–5. [http://orchideen-journal.de/permalink/OrchideenJournal_Vol6_4.pdf]</p>
<p>Vos, P., Hogers, R., Bleeker, M., Reijans, M., van de Lee, T., Hornes, M., Frijters, A., Pot, J., Peleman, J., Kuiper, M. & Zabeau, M. (1995) AFLP: a new technique for DNA fingerprinting. <em>Nucleic Acids Research</em> 11: 4407–4414.</p>
<p>Wang, M., Lan, S.R. & Liu, Z.J. (2017) <em>Paphiopedilum notatisepalum</em>, a new species of slipper orchid (Cypripedioideae, Orchidaceae) from China based on morphological and DNA evidence. <em>Phytotaxa</em> 302 (2): 156–164. https://doi.org/10.11646/phytotaxa.302.2.5</p>