Abstract
A new subspecies of Zingiber ultralimitale Ardiyani & A.D.Poulsen was found at Matarombeo, South-East Sulawesi. Zingiber ultralimitale subsp. matarombeoense Ardiyani & Ardi, was described, illustrated and barcoded using four barcoding loci (rbcL, trnH-psbA, Internal Transcribed Spacer-ITS, and matK). Provisional conservation status assessment indicates a Critical Endangered (CR) status. The taxonomic placement into section was determined based on morphology, molecular, and pollen SEM data.
References
<p>Ardiyani, M., Newman, M.F. & Poulsen, A.D. (2017) A new species of <em>Zingiber</em> (Zingiberaceae) east of Wallace’s Line. <em>Gardens Bulletin Singapore</em> 69 (2): 189–199. https://doi.org/10.26492/gbs69(2).2017-04</p>
<p>Baker, J.G. (1894) Scitamineae.<em> In: </em>Hooker, J.D. (ed.) <em>Flora of British India</em>, vol. 6. L Reeve & Co., London, pp. 198–264.</p>
<p>Bentham, G. & Hooker, J.D. fil. (1883) <em>Genera Plantarum</em>, vol. 3 (2). L. Reeve & Co. & Williams & Norgate, London, 811 pp.</p>
<p>Goldstein, J.I., Newbury, D.E., Echlin, P., Joy, D.C., Romig, A.D., Lyman, C.E., Fiori, C. & Lifshin, E. (1992) <em>Scanning Electron Microscopy and X-ray Microanalysis: A text for Biologist, Materials Scientists and Cytologists.</em> <em>2<sup>nd</sup> ed</em>. Plemun Press, New York, 820 pp. https://doi.org/10.1007/978-1-4613-0491-3</p>
<p>Horaninov, P. (1862) <em>Prodromus monographiae Scitaminearum</em>. Academia Cesareae Scientiarum, St. Petersburg, 45 pp.</p>
<p>IUCN. (2012) <em>IUCN Red List Categories and Criteria: Version 3.1</em>. Second edition. Gland, Switzerland and Cambridge, UK: IUCN. iv+ 32 pp.</p>
<p>Kress, W.J. & Erickson, D.L. (2007) A two-locus global DNA barcode for land plants: The coding <em>rbc</em>L gene complements the non-coding <em>trn</em>H-<em>psb</em>A spacer region. <em>PLoS ONE</em> 2: e508. https://doi.org/10.1371/journal.pone.0000508</p>
<p>Kress, W.J., Prince, L.M. & Williams, K.J. (2002) The phylogeny and a new classification of the gingers (Zingiberaceae): evidence from molecular data. <em>American Journal of Botany</em> 89: 1682–1696. https://doi.org/10.3732/ajb.89.10.1682</p>
<p>Linnaeus, C.V., Wildenow, K.L, Link, H.F. & Dietrich, A. (1831) <em>Species plantarum, exhibentes plantas rite cognitas ad genera relatas cum differentiis specificis, nominibus trivialibus, synonymis selectis, locis natalibus secundum systema sexuale digestas. </em>G.C. Nauck, Berlin. https://doi.org/10.5962/bhl.title.7081</p>
<p>Miller, P. (1754) <em>The Gardeners Dictionary</em>. ed. 4. vol. 3. Printed for the author, London.</p>
<p>Roscoe, W. (1807) A New Arrangement of the Plants of the Monandrian Class usually called Scitaminae. <em>Trans. Linn. Soc. London</em> 8: 348. https://doi.org/10.1111/j.1096-3642.1807.tb00321.x</p>
<p>Schumann, K. (1904) Zingiberaceae. <em>In</em>: Engler, H.G.A. (ed.) <em>Das Pflanzenreich</em>, IV(46), fas. 20. pp. 1–458. [Leipzig: W. Engelmann]</p>
<p>Smith, J.E. (1831) <em>Exotic Botany: Consisting of Coloured Figures, and Scientific Descriptions of such New, Beautiful, or Rare Plants as are Worthy of Cultivation in the Gardens of Britain; with Remarks on Their Qualities, History, and Requisite Modes of Treatment. </em>R. Taylor & Co, London.</p>
<p>Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. <em>Molecular Biology and Evolution</em> 30: 2725–2729. https://doi.org/10.1093/molbev/mst197</p>
<p>Theerakulpisut, P., Triboun, P., Mahakham, W., Maensiri, D., Khampila, J. & Chantaranothai, P. (2012) Phylogeny of the genus <em>Zingiber </em>(Zingiberaceae) based on nuclear ITS sequence data. <em>Kew Bulletin</em> 67: 389–395. https://doi.org/10.1007/s12225-012-9368-2</p>
<p>Theilade, I., Mærsk-Møller, M.L., Theilade, J. & Larsen, K. (1993) Pollen morphology and structure of <em>Zingiber </em>(Zingiberaceae). <em>Grana </em>32: 338–342. https://doi.org/10.1080/00173139309428961</p>
<p>Thomas, D.C., Bour, A. & Ardi, W.H. (2018) <em>Begonia </em>of the Matarombeo karst, Southeast Sulawesi, Indonesia, including two new species. <em>Gardens’ Bulletin Singapore </em>70 (1): 161–174. https://doi.org/10.26492/gbs70(1).2018-15</p>
<p>Wallich, N. (1830) <em>Plantae Asiaticae rariores, or, Descriptions and figures of a select number of unpublished East Indian plants, vol. 1</em>. London: Treuttel and Würtz. https://doi.org/10.5962/bhl.title.468</p>
<p>Zingiberaceae Resource Centre (2017) Royal Botanic Garden Edinburgh. Available from: http://padme.rbge.org.uk/ZRC/welcome (accessed 26 March 2020)</p>
<p>Baker, J.G. (1894) Scitamineae.<em> In: </em>Hooker, J.D. (ed.) <em>Flora of British India</em>, vol. 6. L Reeve & Co., London, pp. 198–264.</p>
<p>Bentham, G. & Hooker, J.D. fil. (1883) <em>Genera Plantarum</em>, vol. 3 (2). L. Reeve & Co. & Williams & Norgate, London, 811 pp.</p>
<p>Goldstein, J.I., Newbury, D.E., Echlin, P., Joy, D.C., Romig, A.D., Lyman, C.E., Fiori, C. & Lifshin, E. (1992) <em>Scanning Electron Microscopy and X-ray Microanalysis: A text for Biologist, Materials Scientists and Cytologists.</em> <em>2<sup>nd</sup> ed</em>. Plemun Press, New York, 820 pp. https://doi.org/10.1007/978-1-4613-0491-3</p>
<p>Horaninov, P. (1862) <em>Prodromus monographiae Scitaminearum</em>. Academia Cesareae Scientiarum, St. Petersburg, 45 pp.</p>
<p>IUCN. (2012) <em>IUCN Red List Categories and Criteria: Version 3.1</em>. Second edition. Gland, Switzerland and Cambridge, UK: IUCN. iv+ 32 pp.</p>
<p>Kress, W.J. & Erickson, D.L. (2007) A two-locus global DNA barcode for land plants: The coding <em>rbc</em>L gene complements the non-coding <em>trn</em>H-<em>psb</em>A spacer region. <em>PLoS ONE</em> 2: e508. https://doi.org/10.1371/journal.pone.0000508</p>
<p>Kress, W.J., Prince, L.M. & Williams, K.J. (2002) The phylogeny and a new classification of the gingers (Zingiberaceae): evidence from molecular data. <em>American Journal of Botany</em> 89: 1682–1696. https://doi.org/10.3732/ajb.89.10.1682</p>
<p>Linnaeus, C.V., Wildenow, K.L, Link, H.F. & Dietrich, A. (1831) <em>Species plantarum, exhibentes plantas rite cognitas ad genera relatas cum differentiis specificis, nominibus trivialibus, synonymis selectis, locis natalibus secundum systema sexuale digestas. </em>G.C. Nauck, Berlin. https://doi.org/10.5962/bhl.title.7081</p>
<p>Miller, P. (1754) <em>The Gardeners Dictionary</em>. ed. 4. vol. 3. Printed for the author, London.</p>
<p>Roscoe, W. (1807) A New Arrangement of the Plants of the Monandrian Class usually called Scitaminae. <em>Trans. Linn. Soc. London</em> 8: 348. https://doi.org/10.1111/j.1096-3642.1807.tb00321.x</p>
<p>Schumann, K. (1904) Zingiberaceae. <em>In</em>: Engler, H.G.A. (ed.) <em>Das Pflanzenreich</em>, IV(46), fas. 20. pp. 1–458. [Leipzig: W. Engelmann]</p>
<p>Smith, J.E. (1831) <em>Exotic Botany: Consisting of Coloured Figures, and Scientific Descriptions of such New, Beautiful, or Rare Plants as are Worthy of Cultivation in the Gardens of Britain; with Remarks on Their Qualities, History, and Requisite Modes of Treatment. </em>R. Taylor & Co, London.</p>
<p>Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. <em>Molecular Biology and Evolution</em> 30: 2725–2729. https://doi.org/10.1093/molbev/mst197</p>
<p>Theerakulpisut, P., Triboun, P., Mahakham, W., Maensiri, D., Khampila, J. & Chantaranothai, P. (2012) Phylogeny of the genus <em>Zingiber </em>(Zingiberaceae) based on nuclear ITS sequence data. <em>Kew Bulletin</em> 67: 389–395. https://doi.org/10.1007/s12225-012-9368-2</p>
<p>Theilade, I., Mærsk-Møller, M.L., Theilade, J. & Larsen, K. (1993) Pollen morphology and structure of <em>Zingiber </em>(Zingiberaceae). <em>Grana </em>32: 338–342. https://doi.org/10.1080/00173139309428961</p>
<p>Thomas, D.C., Bour, A. & Ardi, W.H. (2018) <em>Begonia </em>of the Matarombeo karst, Southeast Sulawesi, Indonesia, including two new species. <em>Gardens’ Bulletin Singapore </em>70 (1): 161–174. https://doi.org/10.26492/gbs70(1).2018-15</p>
<p>Wallich, N. (1830) <em>Plantae Asiaticae rariores, or, Descriptions and figures of a select number of unpublished East Indian plants, vol. 1</em>. London: Treuttel and Würtz. https://doi.org/10.5962/bhl.title.468</p>
<p>Zingiberaceae Resource Centre (2017) Royal Botanic Garden Edinburgh. Available from: http://padme.rbge.org.uk/ZRC/welcome (accessed 26 March 2020)</p>