Abstract
Collection of three sexual morphic fungal species occurring on the leaf surface of three plant species (Dalbergia obtusifolia, Syzygium fluviatile and Ziziphus mauritiana) collected in Yunnan Province, China, have shown that they are morphologically distinct. Phylogenetic analyses of partial sequences of internal transcribed spacer region (ITS) and large subunit rDNA (LSU) placed the three species in Trichomerium (Trichomeriaceae) but are sufficiently different from all known species. Therefore, three new species, Trichomerium multisetosum, T. xishuangbannaense and T. yunnanense are described with detailed, illustrated descriptions and notes.
References
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Crous, P.W., Gams, W., Stalpers, J.A., Robert, V. & Stegehuis, G. (2004) MycoBank: an online initiative to launch mycology into the 21st century. Studies in Mycology 50: 19–22.
Crous, P.W., Wingfield, M.J, Schumacher, R.K., Summerell, B.A., Giraldo, A., Gené, J., Guarro, J., Wanasinghe, D.N., Hyde, K.D., Camporesi, E., Gareth Jones, E.B., Thambugala, K.M., Malysheva, E.F., Malysheva, V.F., Acharya, K., Álvarez, J., Alvarado, P., Assefa, A., Barnes, C.W., Bartlett, J.S., Blanchette, R.A., Burgess, T.I., Carlavilla, J.R., Coetzee, M.P.A., Damm, U., Decock, C.A. den Breeÿen, A., de Vries, B., Dutta, A.K., Holdom, D.G., Latham, S.R., Manjón, J.L., Marincowitz, S., Mirabolfathy, M., Moreno, G., Nakashima, C., Papizadeh, M., Fazeli, S.A.S., Amoozegar, M.A., Romberg, M.K., Shivas, R.G., Stalpers, J.A., Stielow, B., Stukely, M.J.C., Swart, W.J., Tan, Y.P., van der Bank, M., Wood, A.R., Zhang, Y. & Groenewald, J.Z. (2015) Fungal Planet Description Sheets: 281–319. Persoonia 33: 212?289. https://doi.org/10.3767/003158514X685680
Crous, P.W., Wingfield, M.J., Burgess, T.I. et al. (2017) Fungal Planet description sheets: 625–715. Persoonia 39: 270–467. https://doi.org/10.3767/persoonia.2017.39.11
Dissanayake, a.J., Bhunjun, C.S., Maharachchikumbura, S.S.N. & Liu, J.K. (2020) Applied aspects of methods to infer phylogenetic relationships amongst fungi. Mycosphere 11: 2653–2677. https://doi.org/10.5943/mycosphere/11/1/18
Felsenstein, J. (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x
Gueidan, C., Aptroot, A., da Silva, M.E., Badali, H. & Stenroos, S. (2014) A reappraisal of orders and families within the subclass Chaetothyriomycetidae (Eurotiomycetes, Ascomycota). Mycological Progress 13: 1027–1039. https://doi.org/10.1007/s11557-014-0990-2
Hongsanan, S., Tian, Q., Hyde, K.D. & Hu, D.M. (2016) The asexual morph of Trichomerium gloeosporum. Mycosphere 7: 1473–1479. https://doi.org/10.5943/mycosphere/7/9/18
Hyde, K.D., Hongsanan, S., Jeewon, R., et al. (2016) Fungal diversity notes 367–490: taxonomic and phylogenetic contributions to fungal taxa. Fungal Diversity 80: 1–270. https://doi.org/10.1007/s13225-016-0373-x
Index Fungorum (2021) Available from: http://www.indexfungorum.org/Names/Names.asp. (Accessed July 2021)
Kirk, P.M., Cannon, P.F., Minter, D.W. & Stalpers, J.A. (2008) Dictionary of the Fungi. (10thedn). Wallingford, UK.
Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33: 1870–1874. https://doi.org/10.1093/molbev/msw054
Liu, X.Y., Udayanga, D., Luo, Z.L., Chen, L.J., Zhou, D.Q., Su, H.Y. & Hyde, K.D. (2015) Backbone tree for Chaetothyriales with four new species of Minimelanolocus from aquatic habitats. Fungal Biology 119: 1046–1062. https://doi.org/10.1016/j.funbio.2015.08.005
Miller, M.A., Pfeiffer, W. & Schwartz, T. (2012) The CIPRES science gateway: enabling high-impact science for phylogenetics researchers with limited resources. In: Proceedings of the 1st Conference of the Extreme Science and Engineering Discovery Environment: Bridging from the extreme to the campus and beyond. pp. 1–8. https://doi.org/10.1145/2335755.2335836
Nascimento, M.M.F., Selbmannc, L., Sharifyniab, S., Al-Hatmib, A.M.S., Voglmayr, H., Vicente, V.A., Deng, S.W., Kargl, A., Moussa, T.A.A., Al-Zahrani, H.S., Almaghrabi, O.A. & de Hoog, G.S. (2016) Arthrocladium, an unexpected human opportunist in Trichomeriaceae (Chaetothyriales). Fungal Biology 120: 207–218. https://doi.org/10.1016/j.funbio.2015.08.018
Maharachchikumbura, S.S.N., Haituk, S., Pakdeeniti, P., Al-Sadi, A.M., Hongsanan, S., Chomnunti, P. & Cheewangkoon, R. (2018) Phaeosaccardinula coffeicola and Trichomerium chiangmaiensis, two new species of Chaetothyriales (Eurotiomycetes) from Thailand. Mycosphere 9: 769–778. https://doi.org/10.5943/mycosphere/9/4/5
Phillips, A.J.L., Alves, A., Pennycook, S.R., Johnston, P.R., Ramaley, A., Akulov, A. & Crous, P.W. (2008) Resolving the phylogenetic and taxonomic status of dark-spored teleomorph genera in the Botryosphaeriaceae. Persoonia 21: 29–55. https://doi.org/10.3767/003158508X340742
Réblová, M., Untereiner, W.A. & Réblová, K. (2013) Novel evolutionary lineages revealed in the Chaetothyriales (Fungi) based on multi-gene phylogenetic analyses and comparison of ITS secondary structure. PLoS ONE 8: e63547. https://doi.org/10.1371/journal.pone.0063547
Spegazzini, C. (1918) Notas micológicas. Physis Revista de la Sociedad Argentina de Ciencias Naturales 4: 281–295.
Stamatakis, A. (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30: 1312–1313. https://doi.org/10.1093/bioinformatics/btu033
Sun, W., Su, L., Yang, S., Sun, J., Liu, B., Fu, R., Wu, B., Liu, X., Cai, L., Guo, L. & Xiang, M. (2020) Unveiling the Hidden Diversity of Rock-Inhabiting Fungi: Chaetothyriales from China. Journal of Fungi 6: 187. https://doi.org/10.3390/jof6040187
Swofford, D.L. (2002) PAUP: Phylogenetic analysis using parsimony, version 4.0b10. Sinauer Associates, Sunderland.
Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand, D.H., Sninsky, J.J. & White, T.J. (eds.) PCR protocols: a guide to methods and applications. San Diego: Academic Press, pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
Wijayawardene, N.N., Hyde, K.D., Al-Ani, L.K.T., Tedersoo, L., Rajeshkumar, K.C., Zhao, R.L., Aptroot, A., Leontyev, D.V., Saxena, R.K., Tokarev, Y.S., Dai, D.Q., Letcher, P.M., Stephenson, S.L., Ertz, D., Lumbsch, H.T., Haelewaters, D., Bensch, K., Madrid, H., Kukwa, M., Issi, I.V., Lateef, A.A., Phillips, A.J.L., Selbmann, L., Pfiegler, W.P., Horváth, E., Pereira, O.L., Kirk, P.M., Kola?íková, Z., Raja, H.A., Radek, R., Papp, V., Dima, B., Ma, J., Malosso, E., Takamatsu, S., Rambold, G., Gannibal, P.B., Triebel, D., Gautam, A.K., Avasthi, S., Suetrong, S., Timdal, E., Fryar, S.C., Delgado, G., Réblová, M., Doilom, M., Dolatabadi, S., Paw?owska, J., Humber, R.A., Kodsueb, R., Sánchez-Castro, I., Goto, B.T., Silva, D.K.A., de Souza, F.A., Oehl, F., Santos da Silva, G.A., Silva, I.R., B?aszkowski, J., Jobim, K., Maia, L.C., Barbosa, F.R., Fiuza, P.O., Divakar, P.K., Shenoy, B.D., Castañeda-Ruiz, R.F., Somrithipo, S., Karunarathna, S.C., Tibpromma, S., Mortimer, P.E., Wanasinghe, D.N., Phookamsak, R., Xu, J.C., Wang, Y., Fenghua, T., Alvarado, P., Li, D.W., Kušan, I., Mato?ec, N., Maharachchikumbura, S.S.N., Papizadeh, M., Heredia, G., Wartchow, F., Bakhshi, M., Boehm, E., Youssef, N., Hustad, V.P., Lawrey, J.D., Santiago, ALCMA, Bezerra, J.D.P., Souza-Motta, C.M., Firmino, A.L., Tian, Q., Houbraken, J., Hongsanan, S., Tanaka, K., Dissanayake, A.J., Monteiro, J.S., Grossart, H.P., Suija, A., Weerakoon, G., Etayo, J., Tsurykau, A., Kuhnert, E., Vázquez, V., Mungai, P., Damm, U., Li, Q.R., Zhang, H., Boonmee, S., Lu, Y.Z., Becerra, A.G., Kendrick, B., Brearley, F.Q., Motiej?naitë, J., Sharma, B., Khare, R., Gaikwad, S., Wijesundara, D.S.A., Tang, L.Z., He, M.Q., Flakus, A., RodriguezFlakus, P., Zhurbenko, M.P., McKenzie, E.H.C., Stadler, M., Bhat, D.J., Liu, J.K., Raza, M., Jeewon, R., Nassonova, E.S., Prieto, M., Jayalal, R.G.U., Yurkov, A., Schnittler, M., Shchepin, O.N., Novozhilov, Y.K., Liu, P., Cavender, J.C., Kang, Y.Q., Mohammad, S., Zhang, L.F., Xu, R.F., Li, Y.M., Dayarathne, M.C., Ekanayaka, A.H., Wen, T.C., Deng, C.Y. & Navathe, S. (2020) Outline of Fungi and fungus-like taxa. Mycosphere 11: 1060–1456. https://doi.org/10.5943/mycosphere/11/1/8