Abstract
Phaeoacremonium species are important pathogens with a worldwide distribution and diverse host and are commonly associated with brown wood streaking, dieback and Esca disease of plants and phaeohyphomycosis of humans. In this study, three sexual taxa, representing two Phaeoacremonium species, were collected from dead wood from Guangdong and Guizhou Provinces in China. Based on the morphological characters and phylogenetic analyses of a combined ACT and TUB2 sequence data, they are identified as Phaeoacremonium fusiformostromum sp. nov., and P. croatiense, which is a new record from China with its first sexual morph. Detailed descriptions and illustrations of these two species are provided, and an updated phylogenetic tree for all Phaeoacremonium species is also given.
References
<p>Ajello, L., Georg, L.K., Steigbigel, R.T. & Wang, C.J.K. (1976) A case of Phaeohyphomycosis caused by a new species of <em>Phialophora</em>. <em>Mycologia</em> 66 (3): 490–498. https://doi.org/10.1080/00275514.1974.12019630</p>
<p>Ariyawansa, H.A., Hyde, K.D., Jayasiri, S.C., Buyck, B., Chethana, K.W.T., Dai, D.Q., Dai, Y.C., Daranagama, D.A., Jayawardena, R.S., Lücking, R., Ghobad-Nejhad, M., Niskanen, T., Thambugala, K.M., Voigt, K., Zhao, R.L., Li, G.-J., Doilom, M., Boonmee, S., Yang, Z.L., Cai, Q., Cui, Y.-Y., Bahkali, A.H., Chen, J., Cui, B.K., Chen, J.J., Dayarathne, M.C., Dissanayake, A.J., Ekanayaka, A.H., Hashimoto, A., Hongsanan, S., Jones, E.B.G., Larsson, E., Li, W.J., Li, Q.-R., Liu, J.K., Luo, Z.L., Maharachchikumbura, S.S.N., Mapook, A., McKenzie, E.H.C., Norphanphoun, C., Konta, S., Pang, K.L., Perera, R.H., Phookamsak, R., Phukhamsakda, C., Pinruan, U., Randrianjohany, E., Singtripop, C., Tanaka, K., Tian, C.M., Tibpromma, S., Abdel-Wahab, M.A., Wanasinghe, D.N., Wijayawardene, N.N., Zhang, J.-F., Zhang, H., Abdel-Aziz, F.A., Wedin, M., Westberg, M., Ammirati, J.F., Bulgakov, T.S., Lima, D.X., Callaghan, T.M., Callac, P., Chang, C.-H., Coca, L.F., Dal-Forno, M., Dollhofer, V., Fliegerová, K., Greiner, K., Griffith, G.W., Ho, H.-M., Hofstetter, V., Jeewon, R., Kang, J.C., Wen, T.-C., Kirk, P.M., Kytövuori, I., Lawrey, J.D., Xing, J., Li, H., Liu, Z.Y., Liu, X.Z., Liimatainen, K., Lumbsch, H.T., Matsumura, M., Moncada, B., Nuankaew, S., Parnmen, S., de Azevedo Santiago, A.L.C.M., Sommai, S., Song, Y., de Souza, C.A.F., de Souza-Motta, C.M., Su, H.Y., Suetrong, S., Wang, Y., Wei, S.-F., Wen, T.C., Yuan, H.S., Zhou, L.W., Réblová, M., Fournier, J., Camporesi, E., Luangsa-ard, J.J., Tasanathai, K., Khonsanit, A., Thanakitpipattana, D., Somrithipol, S., Diederich, P., Millanes, A.M., Common, R.S., Stadler, M., Yan, J.Y., Li, X., Lee, H.W., Nguyen, T.T.T., Lee, H.B., Battistin, E., Marsico, O., Vizzini, A., Vila, J., Ercole, E., Eberhardt, U., Simonini, G., Wen, H.-A., Chen, X.-H., Miettinen, O., Spirin, V. & Hernawati (2015) Fungal diversity notes 111–252—taxonomic and phylogenetic contributions to fungal taxa. <em>Fungal Diversity</em> 75 (1): 27–274. https://doi.org/10.1007/s13225-015-0346-5</p>
<p>Baddley, J.W., Mostert, L., Summerbell, R.C. & Moser, S.A. (2006) <em>Phaeoacremonium parasiticum</em> infections confirmed by beta-tubulin sequence analysis of case isolates. <em>Journal of Clinical Microbiology </em>44 (6): 2207–2211. https://doi.org/10.1128/JCM.00205-06</p>
<p>Belkin, A., Summerbell, R.C., Krajden, S. & Scott, J. (2020) The type isolate of a rare <em>Phaeoacremonium</em> species as a cause of human eumycetoma. <em>Medical Mycology Case Reports</em> 30: 5–7. https://doi.org/10.1016/j.mmcr.2020.08.002</p>
<p>Berlese, A.N. (1900) <em>Icones Fungorum. Pyrenomycetes. Sphaeriaceae. Allantosporae.</em> 3 (1–2): 1–52.</p>
<p>Calabon, M.S., Jones, E.B.G., Boonmee, S., Doilom, M., Lumyong, S. & Hyde, K.D. (2021) Five novel freshwater ascomycetes indicate high undiscovered diversity in lotic habitats in Thailand. <em>Journal of Fungi</em> 7 (2). https://doi.org/10.3390/jof7020117</p>
<p>Carbone, I. & Kohn, L.M. (1999) A Method for Designing Primer Sets for Speciation Studies in Filamentous Ascomycetes. <em>Mycologia</em> 91 (3): 553–556. https://doi.org/10.2307/3761358</p>
<p>Cloete, M., Fourie, P.H., Damm, U., Crous, P.W. & Mostert, L. (2011) Fungi associated with die-back symptoms of apple and pear trees, a possible inoculum source of grapevine trunk disease pathogens. <em>Phytopathol Mediterr</em> 50: S176-S190</p>
<p>Crous, P.W., Gams, W., Stalpers, J.A., Robert, V. & Stegehuis, G. (2004) MycoBank: an online initiative to launch mycology into the 21st century. <em>Studies in Mycology</em> (50): 19–22</p>
<p>Crous, P.W., Gams, W., Wingfield, M.J. & vanWyk, P.S. (1996) <em>Phaeoacremonium</em> gen nov associated with wilt and decline diseases of woody hosts and human infections. <em>Mycologia</em> 88 (5): 786–796. https://doi.org/10.1080/00275514.1996.12026716</p>
<p>Crous, P.W., Wingfield, M.J., Burgess, T.I., Hardy, G.E., Crane, C., Barrett, S., Cano-Lira, J.F., Le Roux, J.J., Thangavel, R., Guarro, J., Stchigel, A.M., Martin, M.P., Alfredo, D.S., Barber, P.A., Barreto, R.W., Baseia, I.G., Cano-Canals, J., Cheewangkoon, R., Ferreira, R.J., Gene, J., Lechat, C., Moreno, G., Roets, F., Shivas, R.G., Sousa, J.O., Tan, Y.P., Wiederhold, N.P., Abell, S.E., Accioly, T., Albizu, J.L., Alves, J.L., Antoniolli, Z.I., Aplin, N., Araujo, J., Arzanlou, M., Bezerra, J.D., Bouchara, J.P., Carlavilla, J.R., Castillo, A., Castroagudin, V.L., Ceresini, P.C., Claridge, G.F., Coelho, G., Coimbra, V.R., Costa, L.A., da Cunha, K.C., da Silva, S.S., Daniel, R., de Beer, Z.W., Duenas, M., Edwards, J., Enwistle, P., Fiuza, P.O., Fournier, J., Garcia, D., Gibertoni, T.B., Giraud, S., Guevara-Suarez, M., Gusmao, L.F., Haituk, S., Heykoop, M., Hirooka, Y., Hofmann, T.A., Houbraken, J., Hughes, D.P., Kautmanova, I., Koppel, O., Koukol, O., Larsson, E., Latha, K.P., Lee, D.H., Lisboa, D.O., Lisboa, W.S., Lopez-Villalba, A., Maciel, J.L., Manimohan, P., Manjon, J.L., Marincowitz, S., Marney, T.S., Meijer, M., Miller, A.N., Olariaga, I., Paiva, L.M., Piepenbring, M., Poveda-Molero, J.C., Raj, K.N., Raja, H.A., Rougeron, A., Salcedo, I., Samadi, R., Santos, T.A., Scarlett, K., Seifert, K.A., Shuttleworth, L.A., Silva, G.A., Silva, M., Siqueira, J.P., Souza-Motta, C.M., Stephenson, S.L., Sutton, D.A., Tamakeaw, N., Telleria, M.T., Valenzuela-Lopez, N., Viljoen, A., Visagie, C.M., Vizzini, A., Wartchow, F., Wingfield, B.D., Yurchenko, E., Zamora, J.C. & Groenewald, J.Z. (2016) Fungal Planet description sheets: 469–557. <em>Persoonia</em> 37: 218–403. https://doi.org/10.3767/003158516X694499</p>
<p>da Silva, M.A., Correia, K.C., Barbosa, M.A.G., Câmara, M.P.S., Gramaje, D. & Michereff, S.J. (2017) Characterization of <em>Phaeoacremonium</em> isolates associated with Petri disease of table grape in Northeastern Brazil, with description of <em>Phaeoacremonium nordesticola</em> <em>sp. nov.</em> <em>European Journal of Plant Pathology</em> 149 (3): 695–709. https://doi.org/10.1007/s10658-017-1219-4</p>
<p>Damm, U., Mostert, L., Crous, P.W. & Fourie, P.H. (2008) Novel <em>Phaeoacremonium </em>species associated with necrotic wood of Prunus trees. <em>Persoonia</em> 20: 87–102. https://doi.org/10.3767/003158508X324227</p>
<p>Dissanayake, A.J., Bhunjun, C.S., Maharachchikumbura, S.S.M. & Liu, J.K. (2020) Applied aspects of methods to infer phylogenetic relationships amongst fungi. <em>Mycosphere</em> 11 (1): 2652–2676. https://doi.org/10.5943/mycosphere/11/1/18</p>
<p>Dupont, J., Laloui, W., Magnin, S., Larignon, P. & Roquebert, M.-F. (2000) <em>Phaeoacremonium viticola</em>, a new species associated with Esca disease of grapevine in France. <em>Mycologia</em> 92 (3): 499–504. https://doi.org/10.1080/00275514.2000.12061185</p>
<p>Espargham, N., Mohammadi, H. & Gramaje, D. (2020) A Survey of Trunk Disease Pathogens within Citrus Trees in Iran. <em>Plants</em> 9 (6). https://doi.org/10.3390/plants9060754</p>
<p>Essakhi, S., Mugnai, L., Crous, P.W., Groenewald, J.Z. & Surico, G. (2008) Molecular and phenotypic characterisation of novel <em>Phaeoacremonium </em>species isolated from esca diseased grapevines. <em>Persoonia</em> 21: 119–134. https://doi.org/10.3767/003158508X374385</p>
<p>Glez-Pena, D., Gomez-Blanco, D., Reboiro-Jato, M., Fdez-Riverola, F. & Posada, D. (2010) ALTER: program-oriented conversion of DNA and protein alignments. <em>Nucleic Acids Research</em> 38 (Web Server issue): W14–W18. https://doi.org/10.1093/nar/gkq321</p>
<p>Graham, A.B., Johnston, P.R. & Weir, B.S. (2009) Three new <em>Phaeoacremonium</em> species on grapevines in New Zealand. <em>Australasian Plant Pathology</em> 38 (5): 505. https://doi.org/10.1071/AP09035</p>
<p>Gramaje, D., Agusti-Brisach, C., Perez-Sierra, A., Moralejo, E., Olmo, D., Mostert, L., Damm, U. & Armengol, J. (2012) Fungal trunk pathogens associated with wood decay of almond trees on Mallorca (Spain). <em>Persoonia</em> 28: 1–13. https://doi.org/10.3767/003158512X626155</p>
<p>Gramaje, D., Leon, M., Perez-Sierra, A., Burgess, T. & Armengol, J. (2014) New <em>Phaeoacremonium</em> species isolated from sandalwood trees in Western Australia. <em>IMA Fungus </em>5 (1): 67–77. https://doi.org/10.5598/imafungus.2014.05.01.08</p>
<p>Gramaje, D., Mostert, L., Groenewald, J.Z. & Crous, P.W. (2015) <em>Phaeoacremonium</em>: from esca disease to phaeohyphomycosis. <em>Fungal Biology</em> 119 (9): 759–783. https://doi.org/10.1016/j.funbio.2015.06.004</p>
<p>Groenewald, M., Kang, J.-C., Crous, P.W. & Gams, W. (2001) ITS and β-tubulin phylogeny of <em>Phaeoacremonium</em> and <em>Phaeomoniella</em> species. <em>Mycological Research</em> 105 (6): 651–657. https://doi.org/10.1017/S0953756201004282</p>
<p>Hall, T.A. (1999) BioEdit: a use-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. <em>Nucleic Acids Symposium Series</em> 41: 95–98</p>
<p>Huang, S.K., Jeewon, R., Hyde, K.D., Bhat, D.J., Putarak, C. & Wen, T.C. (2018) Beta-tubulin and Actin gene phylogeny supports <em>Phaeoacremonium</em> <em>ovale</em> as a new species from freshwater habitats in China. <em>MycoKeys</em> (41): 1–15. https://doi.org/10.3897/mycokeys.41.27536</p>
<p>Huelsenbeck, J.P. & Ronquist, F. (2001) MRBAYES: Bayesian inference of phylogenetic trees. <em>Bioinformatics</em> 17 (8): 754–755. https://doi.org/10.1093/bioinformatics/17.8.754</p>
<p>Hyde, K.D., Norphanphoun, C., Maharachchikumbura, S.S.N., Bhat, D.J., Jones, E.B.G., Bundhun, D., Chen, Y.J., Bao, D.F., Boonmee, S., Calabon, M.S., Chaiwan, N., Chethana, K.W.T., Dai, D.Q., Dayarathne, M.C., Devadatha, B., Dissanayake, A.J., Dissanayake, L.S., Doilom, M., Dong, W., Fan, X.L., Goonasekara, I.D., Hongsanan, S., Huang, S.K., Jayawardena, R.S., Jeewon, R., Karunarathna, A., Konta, S., Kumar, V., Lin, C.G., Liu, J.K., Liu, N.G., Luangsaard, J., Lumyong, S., Luo, Z.L., Marasinghe, D.S., McKenzie, E.H.C., Niego, A.G.T., Niranjan, M., Perera, R.H., Phukhamsakda, C., Rathnayaka, A.R., Samarakoon, M.C., Samarakoon, S.M.B.C., Sarma, V.V., Senanayake, I.C., Shang, Q.J., Stadler, M., Tibpromma, S., Wanasinghe, D.N., Wei, D.P., Wijayawardene, N.N., Xiao, Y.P., Yang, J., Zeng, X.Y., Zhang, S.N. & Xiang, M.M. (2020) Refined families of Sordariomycetes. <em>Mycosphere</em> 11 (1): 305–1059. https://doi.org/10.5943/mycosphere/11/1/7</p>
<p>Index Fungorum (2021) Available from: www.indexfungorum.org/Names/Names.asp (accessed 13 May 2021)</p>
<p>Jayasiri, S.C., Hyde, K.D., Ariyawansa, H.A., Bhat, J., Buyck, B., Cai, L., Dai, Y.-C., Abd-Elsalam, K.A., Ertz, D., Hidayat, I., Jeewon, R., Jones, E.B.G., Bahkali, A.H., Karunarathna, S.C., Liu, J.-K., Luangsa-ard, J.J., Lumbsch, H.T., Maharachchikumbura, S.S.N., McKenzie, E.H.C., Moncalvo, J.-M., Ghobad-Nejhad, M., Nilsson, H., Pang, K.-L., Pereira, O.L., Phillips, A.J.L., Raspé, O., Rollins, A.W., Romero, A.I., Etayo, J., Selçuk, F., Stephenson, S.L., Suetrong, S., Taylor, J.E., Tsui, C.K.M., Vizzini, A., Abdel-Wahab, M.A., Wen, T.-C., Boonmee, S., Dai, D.Q., Daranagama, D.A., Dissanayake, A.J., Ekanayaka, A.H., Fryar, S.C., Hongsanan, S., Jayawardena, R.S., Li, W.-J., Perera, R.H., Phookamsak, R., de Silva, N.I., Thambugala, K.M., Tian, Q., Wijayawardene, N.N., Zhao, R.-L., Zhao, Q., Kang, J.-C. & Promputtha, I. (2015) The Faces of Fungi database: fungal names linked with morphology, phylogeny and human impacts. <em>Fungal Diversity</em> 74 (1): 3–18. https://doi.org/10.1007/s13225-015-0351-8</p>
<p>Jayawardena, R.S., Hyde, K.D., Jeewon, R., Ghobad-Nejhad, M., Wanasinghe, D.N., Liu, N., Phillips, A.J.L., Oliveira-Filho, J.R.C., da Silva, G.A., Gibertoni, T.B., Abeywikrama, P., Carris, L.M., Chethana, K.W.T., Dissanayake, A.J., Hongsanan, S., Jayasiri, S.C., McTaggart, A.R., Perera, R.H., Phutthacharoen, K., Savchenko, K.G., Shivas, R.G., Thongklang, N., Dong, W., Wei, D., Wijayawardena, N.N. & Kang, J.-C. (2019) One stop shop II: taxonomic update with molecular phylogeny for important phytopathogenic genera: 26–50 (2019). <em>Fungal Diversity</em> 94 (1): 41–129. https://doi.org/10.1007/s13225-019-00418-5</p>
<p>Jeewon, R. & Hyde, K.D. (2016) Establishing species boundaries and new taxa among fungi: recommendations to resolve taxonomic ambiguities. <em>Mycosphere</em> 7 (11): 1669–1677. https://doi.org/10.5943/mycosphere/7/11/4</p>
<p>Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. <em>Molecular Biology and Evolution</em> 30 (4): 772–780. https://doi.org/10.1093/molbev/mst010</p>
<p>Liu, J.K., Chomnunti, P., Lei, C., Phookamsak, R., Chukeatirote, E., Jones, E.B.G., Moslem, M. & Hyde, K. (2010) Phylogeny and morphology of <em>Neodeightonia palmicola</em> <em>sp. nov.</em> from palms. <em>Sydowia</em> 62: 261–276</p>
<p>Marin-Felix, Y., Hernandez-Restrepo, M., Wingfield, M.J., Akulov, A., Carnegie, A.J., Cheewangkoon, R., Gramaje, D., Groenewald, J.Z., Guarnaccia, V., Halleen, F., Lombard, L., Luangsa-Ard, J., Marincowitz, S., Moslemi, A., Mostert, L., Quaedvlieg, W., Schumacher, R.K., Spies, C.F.J., Thangavel, R., Taylor, P.W.J., Wilson, A.M., Wingfield, B.D., Wood, A.R. & Crous, P.W. (2019) Genera of phytopathogenic fungi: GOPHY 2. <em>Studies in Mycology</em> 92: 47–133. https://doi.org/10.1016/j.simyco.2018.04.002</p>
<p>Mostert, L., Groenewald, J.Z., Summerbell, R.C., Gams, W. & Crous, P.W. (2006a) Taxonomy and pathology of <em>Togninia</em> (Diaporthales) and its <em>Phaeoacremonium</em> anamorphs. <em>Studies in Mycology</em> 54 (54): 1–113. https://doi.org/10.3114/sim.54.1.1</p>
<p>Mostert, L., Groenewald, J.Z., Summerbell, R.C., Robert, V., Sutton, D.A., Padhye, A.A. & Crous, P.W. (2005) Species of <em>Phaeoacremonium</em> associated with infections in humans and environmental reservoirs in infected woody plants. <em>Journal of Clinical Microbiology</em> 43 (4): 1752–1767. https://doi.org/10.1128/JCM.43.4.1752-1767.2005</p>
<p>Mostert, L., Halleen, F., Fourie, P. & Crous, P.W. (2006b) A review of <em>Phaeoacremonium </em>species involved in Petri disease and esca of grapevines. <em>Phytopathol Mediterr</em> 45: S12–S29. https://doi.org/10.14601/Phytopathol_Mediterr-1844</p>
<p>O’Donnell, K. & Cigelnik, E. (1997) Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus <em>Fusarium</em> are nonorthologous. <em>Molecular Phylogenetics and Evolution</em> 7 (1): 103–116. https://doi.org/10.1006/mpev.1996.0376</p>
<p>Raimondo, M.L., Lops, F. & Carlucci, A. (2014) <em>Phaeoacremonium italicum</em> <em>sp. nov.</em>, associated with esca of grapevine in southern Italy. <em>Mycologia</em> 106 (6): 1119–1126. https://doi.org/10.3852/14-080</p>
<p>Rambaut, A. (2012) FigTree, version 1.4. 2. University of Edinburgh, Edinburgh.</p>
<p>Rannala, B. & Yang, Z. (1996) Probability distribution of molecular evolutionary trees: a new method of phylogenetic inference. <em>Journal of Molecular Evolution</em> 43 (3): 304–311. https://doi.org/10.1007/BF02338839</p>
<p>Réblová, M. (2011) New insights into the systematics and phylogeny of the genus Jattaea and similar fungi of the Calosphaeriales. <em>Fungal Diversity</em> 49 (1): 167–198. https://doi.org/10.1007/s13225-011-0099-8</p>
<p>Réblová, M., Jaklitsch, W.M., Reblova, K. & Stepanek, V. (2015) Phylogenetic reconstruction of the Calosphaeriales and Togniniales using five genes and predicted RNA secondary structures of ITS, and <em>Flabellascus tenuirostris</em> <em>gen. et sp. nov</em>. <em>PLoS One</em> 10 (12): e0144616. https://doi.org/10.1371/journal.pone.0144616</p>
<p>Réblová, M., Miller, A.N., Rossman, A.Y., Seifert, K.A., Crous, P.W., Hawksworth, D.L., Abdel-Wahab, M.A., Cannon, P.F., Daranagama, D.A., De Beer, Z.W., Huang, S.K., Hyde, K.D., Jayawardena, R., Jaklitsch, W., Jones, E.B., Ju, Y.M., Judith, C., Maharachchikumbura, S.S., Pang, K.L., Petrini, L.E., Raja, H.A., Romero, A.I., Shearer, C., Senanayake, I.C., Voglmayr, H., Weir, B.S. & Wijayawarden, N.N. (2016) Recommendations for competing sexual-asexually typified generic names in Sordariomycetes (except Diaporthales, Hypocreales, and Magnaporthales). <em>IMA Fungus</em> 7 (1): 131–153. https://doi.org/10.5598/imafungus.2016.07.01.08</p>
<p>Senanayake, I.C., Rathnayaka, A.R., Marasinghe, D.S., Calabon, M.S., Gentekaki, E., Lee, H.B., Hurdeal, V.G., Pem, D., Dissanayake, L.S., Wijesinghe, S.N., Bundhun, D., Nguyen, T.T., Goonasekara, I.D., Abeywickrama, P.D., Bhunjun, C.S., Jayawardena, R.S., Wanasinghe, D.N., Jeewon, R., Bhat, D.J. & Xiang, M.M. (2020) Morphological approaches in studying fungi: collection, examination, isolation, sporulation and preservation. <em>Mycosphere</em> 11 (1): 2678–2754. https://doi.org/10.5943/mycosphere/11/1/20</p>
<p>Silvestro, D. & Michalak, I. (2012) raxmlGUI: a graphical front-end for RAxML. <em>Organisms Diversity & Evolution</em> 12 (4): 335–337. https://doi.org/10.1007/s13127-011-0056-0</p>
<p>Sohrabi, M., Mohammadi, H., Leon, M., Armengol, J. & Banihashemi, Z. (2020) Fungal pathogens associated with branch and trunk cankers of nut crops in Iran. <em>European Journal of Plant Pathology</em> 157 (2): 327–351. https://doi.org/10.1007/s10658-020-01996-w</p>
<p>Spies, C.F.J., Moyo, P., Halleen, F. & Mostert, L. (2018) <em>Phaeoacremonium</em> species diversity on woody hosts in the Western Cape Province of South Africa. <em>Persoonia</em> 40: 26–62. https://doi.org/10.3767/persoonia.2018.40.02</p>
<p>Swofford, D. (2002) <em>PAUP</em><em>∗</em><em> phylogenetic analysis using parsimony (and other methods)</em>, version 4. Sunderland, MA: Sinauer.</p>
<p>TreeBASE (2021) Available from: https://treebase.org/treebase-web/user/submissionList.html (accessed 13 May 2021)</p>
<p>Úrbez-Torres, J.R., Haag, P., Bowen, P. & O’Gorman, D.T. (2014) Grapevine trunk diseases in British Columbia: Incidence and characterization of the fungal pathogens associated with Esca and Petri Diseases of grapevine. <em>Plant Disease</em> 98 (4): 469–482. https://doi.org/10.1094/PDIS-05-13-0523-RE</p>
<p>Zhaxybayeva, O. & Gogarten, J.P. (2002) Bootstrap, Bayesian probability and maximum likelihood mapping: exploring new tools for comparative genome analyses. <em>BMC Genomics</em> 3: 4. https://doi.org/10.1186/1471-2164-3-4</p>
<p>Ariyawansa, H.A., Hyde, K.D., Jayasiri, S.C., Buyck, B., Chethana, K.W.T., Dai, D.Q., Dai, Y.C., Daranagama, D.A., Jayawardena, R.S., Lücking, R., Ghobad-Nejhad, M., Niskanen, T., Thambugala, K.M., Voigt, K., Zhao, R.L., Li, G.-J., Doilom, M., Boonmee, S., Yang, Z.L., Cai, Q., Cui, Y.-Y., Bahkali, A.H., Chen, J., Cui, B.K., Chen, J.J., Dayarathne, M.C., Dissanayake, A.J., Ekanayaka, A.H., Hashimoto, A., Hongsanan, S., Jones, E.B.G., Larsson, E., Li, W.J., Li, Q.-R., Liu, J.K., Luo, Z.L., Maharachchikumbura, S.S.N., Mapook, A., McKenzie, E.H.C., Norphanphoun, C., Konta, S., Pang, K.L., Perera, R.H., Phookamsak, R., Phukhamsakda, C., Pinruan, U., Randrianjohany, E., Singtripop, C., Tanaka, K., Tian, C.M., Tibpromma, S., Abdel-Wahab, M.A., Wanasinghe, D.N., Wijayawardene, N.N., Zhang, J.-F., Zhang, H., Abdel-Aziz, F.A., Wedin, M., Westberg, M., Ammirati, J.F., Bulgakov, T.S., Lima, D.X., Callaghan, T.M., Callac, P., Chang, C.-H., Coca, L.F., Dal-Forno, M., Dollhofer, V., Fliegerová, K., Greiner, K., Griffith, G.W., Ho, H.-M., Hofstetter, V., Jeewon, R., Kang, J.C., Wen, T.-C., Kirk, P.M., Kytövuori, I., Lawrey, J.D., Xing, J., Li, H., Liu, Z.Y., Liu, X.Z., Liimatainen, K., Lumbsch, H.T., Matsumura, M., Moncada, B., Nuankaew, S., Parnmen, S., de Azevedo Santiago, A.L.C.M., Sommai, S., Song, Y., de Souza, C.A.F., de Souza-Motta, C.M., Su, H.Y., Suetrong, S., Wang, Y., Wei, S.-F., Wen, T.C., Yuan, H.S., Zhou, L.W., Réblová, M., Fournier, J., Camporesi, E., Luangsa-ard, J.J., Tasanathai, K., Khonsanit, A., Thanakitpipattana, D., Somrithipol, S., Diederich, P., Millanes, A.M., Common, R.S., Stadler, M., Yan, J.Y., Li, X., Lee, H.W., Nguyen, T.T.T., Lee, H.B., Battistin, E., Marsico, O., Vizzini, A., Vila, J., Ercole, E., Eberhardt, U., Simonini, G., Wen, H.-A., Chen, X.-H., Miettinen, O., Spirin, V. & Hernawati (2015) Fungal diversity notes 111–252—taxonomic and phylogenetic contributions to fungal taxa. <em>Fungal Diversity</em> 75 (1): 27–274. https://doi.org/10.1007/s13225-015-0346-5</p>
<p>Baddley, J.W., Mostert, L., Summerbell, R.C. & Moser, S.A. (2006) <em>Phaeoacremonium parasiticum</em> infections confirmed by beta-tubulin sequence analysis of case isolates. <em>Journal of Clinical Microbiology </em>44 (6): 2207–2211. https://doi.org/10.1128/JCM.00205-06</p>
<p>Belkin, A., Summerbell, R.C., Krajden, S. & Scott, J. (2020) The type isolate of a rare <em>Phaeoacremonium</em> species as a cause of human eumycetoma. <em>Medical Mycology Case Reports</em> 30: 5–7. https://doi.org/10.1016/j.mmcr.2020.08.002</p>
<p>Berlese, A.N. (1900) <em>Icones Fungorum. Pyrenomycetes. Sphaeriaceae. Allantosporae.</em> 3 (1–2): 1–52.</p>
<p>Calabon, M.S., Jones, E.B.G., Boonmee, S., Doilom, M., Lumyong, S. & Hyde, K.D. (2021) Five novel freshwater ascomycetes indicate high undiscovered diversity in lotic habitats in Thailand. <em>Journal of Fungi</em> 7 (2). https://doi.org/10.3390/jof7020117</p>
<p>Carbone, I. & Kohn, L.M. (1999) A Method for Designing Primer Sets for Speciation Studies in Filamentous Ascomycetes. <em>Mycologia</em> 91 (3): 553–556. https://doi.org/10.2307/3761358</p>
<p>Cloete, M., Fourie, P.H., Damm, U., Crous, P.W. & Mostert, L. (2011) Fungi associated with die-back symptoms of apple and pear trees, a possible inoculum source of grapevine trunk disease pathogens. <em>Phytopathol Mediterr</em> 50: S176-S190</p>
<p>Crous, P.W., Gams, W., Stalpers, J.A., Robert, V. & Stegehuis, G. (2004) MycoBank: an online initiative to launch mycology into the 21st century. <em>Studies in Mycology</em> (50): 19–22</p>
<p>Crous, P.W., Gams, W., Wingfield, M.J. & vanWyk, P.S. (1996) <em>Phaeoacremonium</em> gen nov associated with wilt and decline diseases of woody hosts and human infections. <em>Mycologia</em> 88 (5): 786–796. https://doi.org/10.1080/00275514.1996.12026716</p>
<p>Crous, P.W., Wingfield, M.J., Burgess, T.I., Hardy, G.E., Crane, C., Barrett, S., Cano-Lira, J.F., Le Roux, J.J., Thangavel, R., Guarro, J., Stchigel, A.M., Martin, M.P., Alfredo, D.S., Barber, P.A., Barreto, R.W., Baseia, I.G., Cano-Canals, J., Cheewangkoon, R., Ferreira, R.J., Gene, J., Lechat, C., Moreno, G., Roets, F., Shivas, R.G., Sousa, J.O., Tan, Y.P., Wiederhold, N.P., Abell, S.E., Accioly, T., Albizu, J.L., Alves, J.L., Antoniolli, Z.I., Aplin, N., Araujo, J., Arzanlou, M., Bezerra, J.D., Bouchara, J.P., Carlavilla, J.R., Castillo, A., Castroagudin, V.L., Ceresini, P.C., Claridge, G.F., Coelho, G., Coimbra, V.R., Costa, L.A., da Cunha, K.C., da Silva, S.S., Daniel, R., de Beer, Z.W., Duenas, M., Edwards, J., Enwistle, P., Fiuza, P.O., Fournier, J., Garcia, D., Gibertoni, T.B., Giraud, S., Guevara-Suarez, M., Gusmao, L.F., Haituk, S., Heykoop, M., Hirooka, Y., Hofmann, T.A., Houbraken, J., Hughes, D.P., Kautmanova, I., Koppel, O., Koukol, O., Larsson, E., Latha, K.P., Lee, D.H., Lisboa, D.O., Lisboa, W.S., Lopez-Villalba, A., Maciel, J.L., Manimohan, P., Manjon, J.L., Marincowitz, S., Marney, T.S., Meijer, M., Miller, A.N., Olariaga, I., Paiva, L.M., Piepenbring, M., Poveda-Molero, J.C., Raj, K.N., Raja, H.A., Rougeron, A., Salcedo, I., Samadi, R., Santos, T.A., Scarlett, K., Seifert, K.A., Shuttleworth, L.A., Silva, G.A., Silva, M., Siqueira, J.P., Souza-Motta, C.M., Stephenson, S.L., Sutton, D.A., Tamakeaw, N., Telleria, M.T., Valenzuela-Lopez, N., Viljoen, A., Visagie, C.M., Vizzini, A., Wartchow, F., Wingfield, B.D., Yurchenko, E., Zamora, J.C. & Groenewald, J.Z. (2016) Fungal Planet description sheets: 469–557. <em>Persoonia</em> 37: 218–403. https://doi.org/10.3767/003158516X694499</p>
<p>da Silva, M.A., Correia, K.C., Barbosa, M.A.G., Câmara, M.P.S., Gramaje, D. & Michereff, S.J. (2017) Characterization of <em>Phaeoacremonium</em> isolates associated with Petri disease of table grape in Northeastern Brazil, with description of <em>Phaeoacremonium nordesticola</em> <em>sp. nov.</em> <em>European Journal of Plant Pathology</em> 149 (3): 695–709. https://doi.org/10.1007/s10658-017-1219-4</p>
<p>Damm, U., Mostert, L., Crous, P.W. & Fourie, P.H. (2008) Novel <em>Phaeoacremonium </em>species associated with necrotic wood of Prunus trees. <em>Persoonia</em> 20: 87–102. https://doi.org/10.3767/003158508X324227</p>
<p>Dissanayake, A.J., Bhunjun, C.S., Maharachchikumbura, S.S.M. & Liu, J.K. (2020) Applied aspects of methods to infer phylogenetic relationships amongst fungi. <em>Mycosphere</em> 11 (1): 2652–2676. https://doi.org/10.5943/mycosphere/11/1/18</p>
<p>Dupont, J., Laloui, W., Magnin, S., Larignon, P. & Roquebert, M.-F. (2000) <em>Phaeoacremonium viticola</em>, a new species associated with Esca disease of grapevine in France. <em>Mycologia</em> 92 (3): 499–504. https://doi.org/10.1080/00275514.2000.12061185</p>
<p>Espargham, N., Mohammadi, H. & Gramaje, D. (2020) A Survey of Trunk Disease Pathogens within Citrus Trees in Iran. <em>Plants</em> 9 (6). https://doi.org/10.3390/plants9060754</p>
<p>Essakhi, S., Mugnai, L., Crous, P.W., Groenewald, J.Z. & Surico, G. (2008) Molecular and phenotypic characterisation of novel <em>Phaeoacremonium </em>species isolated from esca diseased grapevines. <em>Persoonia</em> 21: 119–134. https://doi.org/10.3767/003158508X374385</p>
<p>Glez-Pena, D., Gomez-Blanco, D., Reboiro-Jato, M., Fdez-Riverola, F. & Posada, D. (2010) ALTER: program-oriented conversion of DNA and protein alignments. <em>Nucleic Acids Research</em> 38 (Web Server issue): W14–W18. https://doi.org/10.1093/nar/gkq321</p>
<p>Graham, A.B., Johnston, P.R. & Weir, B.S. (2009) Three new <em>Phaeoacremonium</em> species on grapevines in New Zealand. <em>Australasian Plant Pathology</em> 38 (5): 505. https://doi.org/10.1071/AP09035</p>
<p>Gramaje, D., Agusti-Brisach, C., Perez-Sierra, A., Moralejo, E., Olmo, D., Mostert, L., Damm, U. & Armengol, J. (2012) Fungal trunk pathogens associated with wood decay of almond trees on Mallorca (Spain). <em>Persoonia</em> 28: 1–13. https://doi.org/10.3767/003158512X626155</p>
<p>Gramaje, D., Leon, M., Perez-Sierra, A., Burgess, T. & Armengol, J. (2014) New <em>Phaeoacremonium</em> species isolated from sandalwood trees in Western Australia. <em>IMA Fungus </em>5 (1): 67–77. https://doi.org/10.5598/imafungus.2014.05.01.08</p>
<p>Gramaje, D., Mostert, L., Groenewald, J.Z. & Crous, P.W. (2015) <em>Phaeoacremonium</em>: from esca disease to phaeohyphomycosis. <em>Fungal Biology</em> 119 (9): 759–783. https://doi.org/10.1016/j.funbio.2015.06.004</p>
<p>Groenewald, M., Kang, J.-C., Crous, P.W. & Gams, W. (2001) ITS and β-tubulin phylogeny of <em>Phaeoacremonium</em> and <em>Phaeomoniella</em> species. <em>Mycological Research</em> 105 (6): 651–657. https://doi.org/10.1017/S0953756201004282</p>
<p>Hall, T.A. (1999) BioEdit: a use-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. <em>Nucleic Acids Symposium Series</em> 41: 95–98</p>
<p>Huang, S.K., Jeewon, R., Hyde, K.D., Bhat, D.J., Putarak, C. & Wen, T.C. (2018) Beta-tubulin and Actin gene phylogeny supports <em>Phaeoacremonium</em> <em>ovale</em> as a new species from freshwater habitats in China. <em>MycoKeys</em> (41): 1–15. https://doi.org/10.3897/mycokeys.41.27536</p>
<p>Huelsenbeck, J.P. & Ronquist, F. (2001) MRBAYES: Bayesian inference of phylogenetic trees. <em>Bioinformatics</em> 17 (8): 754–755. https://doi.org/10.1093/bioinformatics/17.8.754</p>
<p>Hyde, K.D., Norphanphoun, C., Maharachchikumbura, S.S.N., Bhat, D.J., Jones, E.B.G., Bundhun, D., Chen, Y.J., Bao, D.F., Boonmee, S., Calabon, M.S., Chaiwan, N., Chethana, K.W.T., Dai, D.Q., Dayarathne, M.C., Devadatha, B., Dissanayake, A.J., Dissanayake, L.S., Doilom, M., Dong, W., Fan, X.L., Goonasekara, I.D., Hongsanan, S., Huang, S.K., Jayawardena, R.S., Jeewon, R., Karunarathna, A., Konta, S., Kumar, V., Lin, C.G., Liu, J.K., Liu, N.G., Luangsaard, J., Lumyong, S., Luo, Z.L., Marasinghe, D.S., McKenzie, E.H.C., Niego, A.G.T., Niranjan, M., Perera, R.H., Phukhamsakda, C., Rathnayaka, A.R., Samarakoon, M.C., Samarakoon, S.M.B.C., Sarma, V.V., Senanayake, I.C., Shang, Q.J., Stadler, M., Tibpromma, S., Wanasinghe, D.N., Wei, D.P., Wijayawardene, N.N., Xiao, Y.P., Yang, J., Zeng, X.Y., Zhang, S.N. & Xiang, M.M. (2020) Refined families of Sordariomycetes. <em>Mycosphere</em> 11 (1): 305–1059. https://doi.org/10.5943/mycosphere/11/1/7</p>
<p>Index Fungorum (2021) Available from: www.indexfungorum.org/Names/Names.asp (accessed 13 May 2021)</p>
<p>Jayasiri, S.C., Hyde, K.D., Ariyawansa, H.A., Bhat, J., Buyck, B., Cai, L., Dai, Y.-C., Abd-Elsalam, K.A., Ertz, D., Hidayat, I., Jeewon, R., Jones, E.B.G., Bahkali, A.H., Karunarathna, S.C., Liu, J.-K., Luangsa-ard, J.J., Lumbsch, H.T., Maharachchikumbura, S.S.N., McKenzie, E.H.C., Moncalvo, J.-M., Ghobad-Nejhad, M., Nilsson, H., Pang, K.-L., Pereira, O.L., Phillips, A.J.L., Raspé, O., Rollins, A.W., Romero, A.I., Etayo, J., Selçuk, F., Stephenson, S.L., Suetrong, S., Taylor, J.E., Tsui, C.K.M., Vizzini, A., Abdel-Wahab, M.A., Wen, T.-C., Boonmee, S., Dai, D.Q., Daranagama, D.A., Dissanayake, A.J., Ekanayaka, A.H., Fryar, S.C., Hongsanan, S., Jayawardena, R.S., Li, W.-J., Perera, R.H., Phookamsak, R., de Silva, N.I., Thambugala, K.M., Tian, Q., Wijayawardene, N.N., Zhao, R.-L., Zhao, Q., Kang, J.-C. & Promputtha, I. (2015) The Faces of Fungi database: fungal names linked with morphology, phylogeny and human impacts. <em>Fungal Diversity</em> 74 (1): 3–18. https://doi.org/10.1007/s13225-015-0351-8</p>
<p>Jayawardena, R.S., Hyde, K.D., Jeewon, R., Ghobad-Nejhad, M., Wanasinghe, D.N., Liu, N., Phillips, A.J.L., Oliveira-Filho, J.R.C., da Silva, G.A., Gibertoni, T.B., Abeywikrama, P., Carris, L.M., Chethana, K.W.T., Dissanayake, A.J., Hongsanan, S., Jayasiri, S.C., McTaggart, A.R., Perera, R.H., Phutthacharoen, K., Savchenko, K.G., Shivas, R.G., Thongklang, N., Dong, W., Wei, D., Wijayawardena, N.N. & Kang, J.-C. (2019) One stop shop II: taxonomic update with molecular phylogeny for important phytopathogenic genera: 26–50 (2019). <em>Fungal Diversity</em> 94 (1): 41–129. https://doi.org/10.1007/s13225-019-00418-5</p>
<p>Jeewon, R. & Hyde, K.D. (2016) Establishing species boundaries and new taxa among fungi: recommendations to resolve taxonomic ambiguities. <em>Mycosphere</em> 7 (11): 1669–1677. https://doi.org/10.5943/mycosphere/7/11/4</p>
<p>Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. <em>Molecular Biology and Evolution</em> 30 (4): 772–780. https://doi.org/10.1093/molbev/mst010</p>
<p>Liu, J.K., Chomnunti, P., Lei, C., Phookamsak, R., Chukeatirote, E., Jones, E.B.G., Moslem, M. & Hyde, K. (2010) Phylogeny and morphology of <em>Neodeightonia palmicola</em> <em>sp. nov.</em> from palms. <em>Sydowia</em> 62: 261–276</p>
<p>Marin-Felix, Y., Hernandez-Restrepo, M., Wingfield, M.J., Akulov, A., Carnegie, A.J., Cheewangkoon, R., Gramaje, D., Groenewald, J.Z., Guarnaccia, V., Halleen, F., Lombard, L., Luangsa-Ard, J., Marincowitz, S., Moslemi, A., Mostert, L., Quaedvlieg, W., Schumacher, R.K., Spies, C.F.J., Thangavel, R., Taylor, P.W.J., Wilson, A.M., Wingfield, B.D., Wood, A.R. & Crous, P.W. (2019) Genera of phytopathogenic fungi: GOPHY 2. <em>Studies in Mycology</em> 92: 47–133. https://doi.org/10.1016/j.simyco.2018.04.002</p>
<p>Mostert, L., Groenewald, J.Z., Summerbell, R.C., Gams, W. & Crous, P.W. (2006a) Taxonomy and pathology of <em>Togninia</em> (Diaporthales) and its <em>Phaeoacremonium</em> anamorphs. <em>Studies in Mycology</em> 54 (54): 1–113. https://doi.org/10.3114/sim.54.1.1</p>
<p>Mostert, L., Groenewald, J.Z., Summerbell, R.C., Robert, V., Sutton, D.A., Padhye, A.A. & Crous, P.W. (2005) Species of <em>Phaeoacremonium</em> associated with infections in humans and environmental reservoirs in infected woody plants. <em>Journal of Clinical Microbiology</em> 43 (4): 1752–1767. https://doi.org/10.1128/JCM.43.4.1752-1767.2005</p>
<p>Mostert, L., Halleen, F., Fourie, P. & Crous, P.W. (2006b) A review of <em>Phaeoacremonium </em>species involved in Petri disease and esca of grapevines. <em>Phytopathol Mediterr</em> 45: S12–S29. https://doi.org/10.14601/Phytopathol_Mediterr-1844</p>
<p>O’Donnell, K. & Cigelnik, E. (1997) Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus <em>Fusarium</em> are nonorthologous. <em>Molecular Phylogenetics and Evolution</em> 7 (1): 103–116. https://doi.org/10.1006/mpev.1996.0376</p>
<p>Raimondo, M.L., Lops, F. & Carlucci, A. (2014) <em>Phaeoacremonium italicum</em> <em>sp. nov.</em>, associated with esca of grapevine in southern Italy. <em>Mycologia</em> 106 (6): 1119–1126. https://doi.org/10.3852/14-080</p>
<p>Rambaut, A. (2012) FigTree, version 1.4. 2. University of Edinburgh, Edinburgh.</p>
<p>Rannala, B. & Yang, Z. (1996) Probability distribution of molecular evolutionary trees: a new method of phylogenetic inference. <em>Journal of Molecular Evolution</em> 43 (3): 304–311. https://doi.org/10.1007/BF02338839</p>
<p>Réblová, M. (2011) New insights into the systematics and phylogeny of the genus Jattaea and similar fungi of the Calosphaeriales. <em>Fungal Diversity</em> 49 (1): 167–198. https://doi.org/10.1007/s13225-011-0099-8</p>
<p>Réblová, M., Jaklitsch, W.M., Reblova, K. & Stepanek, V. (2015) Phylogenetic reconstruction of the Calosphaeriales and Togniniales using five genes and predicted RNA secondary structures of ITS, and <em>Flabellascus tenuirostris</em> <em>gen. et sp. nov</em>. <em>PLoS One</em> 10 (12): e0144616. https://doi.org/10.1371/journal.pone.0144616</p>
<p>Réblová, M., Miller, A.N., Rossman, A.Y., Seifert, K.A., Crous, P.W., Hawksworth, D.L., Abdel-Wahab, M.A., Cannon, P.F., Daranagama, D.A., De Beer, Z.W., Huang, S.K., Hyde, K.D., Jayawardena, R., Jaklitsch, W., Jones, E.B., Ju, Y.M., Judith, C., Maharachchikumbura, S.S., Pang, K.L., Petrini, L.E., Raja, H.A., Romero, A.I., Shearer, C., Senanayake, I.C., Voglmayr, H., Weir, B.S. & Wijayawarden, N.N. (2016) Recommendations for competing sexual-asexually typified generic names in Sordariomycetes (except Diaporthales, Hypocreales, and Magnaporthales). <em>IMA Fungus</em> 7 (1): 131–153. https://doi.org/10.5598/imafungus.2016.07.01.08</p>
<p>Senanayake, I.C., Rathnayaka, A.R., Marasinghe, D.S., Calabon, M.S., Gentekaki, E., Lee, H.B., Hurdeal, V.G., Pem, D., Dissanayake, L.S., Wijesinghe, S.N., Bundhun, D., Nguyen, T.T., Goonasekara, I.D., Abeywickrama, P.D., Bhunjun, C.S., Jayawardena, R.S., Wanasinghe, D.N., Jeewon, R., Bhat, D.J. & Xiang, M.M. (2020) Morphological approaches in studying fungi: collection, examination, isolation, sporulation and preservation. <em>Mycosphere</em> 11 (1): 2678–2754. https://doi.org/10.5943/mycosphere/11/1/20</p>
<p>Silvestro, D. & Michalak, I. (2012) raxmlGUI: a graphical front-end for RAxML. <em>Organisms Diversity & Evolution</em> 12 (4): 335–337. https://doi.org/10.1007/s13127-011-0056-0</p>
<p>Sohrabi, M., Mohammadi, H., Leon, M., Armengol, J. & Banihashemi, Z. (2020) Fungal pathogens associated with branch and trunk cankers of nut crops in Iran. <em>European Journal of Plant Pathology</em> 157 (2): 327–351. https://doi.org/10.1007/s10658-020-01996-w</p>
<p>Spies, C.F.J., Moyo, P., Halleen, F. & Mostert, L. (2018) <em>Phaeoacremonium</em> species diversity on woody hosts in the Western Cape Province of South Africa. <em>Persoonia</em> 40: 26–62. https://doi.org/10.3767/persoonia.2018.40.02</p>
<p>Swofford, D. (2002) <em>PAUP</em><em>∗</em><em> phylogenetic analysis using parsimony (and other methods)</em>, version 4. Sunderland, MA: Sinauer.</p>
<p>TreeBASE (2021) Available from: https://treebase.org/treebase-web/user/submissionList.html (accessed 13 May 2021)</p>
<p>Úrbez-Torres, J.R., Haag, P., Bowen, P. & O’Gorman, D.T. (2014) Grapevine trunk diseases in British Columbia: Incidence and characterization of the fungal pathogens associated with Esca and Petri Diseases of grapevine. <em>Plant Disease</em> 98 (4): 469–482. https://doi.org/10.1094/PDIS-05-13-0523-RE</p>
<p>Zhaxybayeva, O. & Gogarten, J.P. (2002) Bootstrap, Bayesian probability and maximum likelihood mapping: exploring new tools for comparative genome analyses. <em>BMC Genomics</em> 3: 4. https://doi.org/10.1186/1471-2164-3-4</p>