Abstract
A fungal strain designated KNU16-007, belonging to the family Botryosphaeriaceae, was isolated from soil in Daegu, Korea. Phylogenetic analyses based on the concatenated nucleotide sequences of the ITS and TEF-1α regions, showed that the isolate resides in a clade together with Diplodia species but occupies a distinct phylogenetic position. Conidial dimensions of strain KNU16-007 (22.7–29.3 μm × 8.9–10.9 μm) or its conidial length:width ratio were significantly differed from those of the closely related D. sapinea, D. intermedia, D. scrobiculata, D. seriata, D. crataegicola, D. rosacearum, and D. citricarpa clearly indicating morphological differences from these species. Detailed descriptions, illustrations, and discussions regarding the morphological and phylogenetic analyses of the closely related species are provided to support the novelty of the isolated species. The results of phylogenetic analysis and morphological observations indicate that strain KNU16-007 represents a novel species in the genus Diplodia, for which the name Diplodia parva sp. nov. is proposed.
References
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Fitch, W.M. (1971) Toward defining the course of evolution: minimum change for a specific tree topology. Systematic Zoology 20 (4): 406–416. https://doi.org/10.2307/2412116
Giambra, S., Piazza, G., Alves, A., Mondello, V., Berbegal, M., Armengol, J. & Burruano, S. (2016) Botryosphaeriaceae species associated with diseased loquat trees in Italy and description of Diplodia rosacearum sp. nov. Mycosphere 7 (7): 978–989. https://doi.org/10.5943/mycosphere/si/1b/9
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Kim, G.Y., Jeon, J.S. & Kim, J.K. (2016) Isolation frequency characteristics of Candida species from clinical specimens. Mycobiology 44 (2): 99–104. https://doi.org/10.5941/MYCO.2016.44.2.99
Kimura, M. (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Molecular Biology and Evolution 16 (2): 111–120. https://doi.org/10.1007/bf01731581
Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33 (7): 1870–1874. https://doi.org/10.1093/molbev/msw054
Lallemand, B., Masi, M., Maddau, L., De Lorenzi, M., Dam, R., Cimmino, A., Moreno Y Banuls, L., Andolfi, A., Kiss, R., Mathieu, V. & Evidente, A. (2012) Evaluation of in vitro anticancer activity of sphaeropsidins A–C, fungal rearranged pimarane diterpenes, and semisynthetic derivatives. Phytochemistry Letters 5 (4): 770–775. https://doi.org/10.1016/j.phytol.2012.08.011
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Lazzizera, C., Frisullo, S., Alves, A., Lopes, J.3 & Phillips, A.J.L. (2008) Phylogeny and morphology of Diplodia species on olives in southern Italy and description of Diplodia olivarum sp. nov. Fungal Diversity 31 (NA): 63–71.
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